Biogenic amine content of rat gastric tissues during reduced gastric circulation

The content of biogenic amines in gastric tissues was studied in rats with celiac trunk stenosis 3 hours, one day, 2 and 7 days after operation. Chronic ischemia of the gastric wall was found to lead to the development of dystrophic changes in the gastric mucosa, accompanied by the decreased catecholamine content and elevated levels of histamine and serotonin.     

The effects of the pathogenic influence of botulin toxin on different types of spinal motor neurons]

The motor neuron cells of the lumbosacral region were investigated in the spinal cord of cat with the local botulin paralysis. Development of this paralysis was followed by reduction of the membrane potential, of the amplitude of antidromic AP, mono- and polysynaptic EPSP, a fall of the input resistance and by an increase in the level of the critical depolarization of the somatic membrane of the phasic motor neurons of the damaged segments in the spinal cord. Excitation of the tonic motor neurons was not greatly altered in the dynamics of local botulism.     

Biogenic amine levels change in the brains of stressed honeybees

Three different stress treatments, CO2 anesthesia, chilling anesthesia, and vertical spin, were applied to test whether honeybee (Apis mellifera) workers express stress responses in rewarding behaviors. In the present work, we defined the rewarding behaviors as the bees flying between the hive and feeder. The results from behavioral observation show that the flight time interval of the rewarding behavior of bee workers, flying between hive and feeder, was longer when they were stressed, suggesting that the stress treatments affected the workers' rewarding behavior. The biogenic amine levels in the workers' brains were measured to examine the rapid biochemical brain response to the stressors. After the chilling anesthesia, the dopamine (DA) and octopamine (OA) levels were significantly decreased; with the CO2 anesthesia for durations of both 2 min and 4 min, only DA showed a significant decrease. In the non-anesthesia treatments, the vertical spin with a velocity of 50 and 60 rpm for 90 s, the DA and OA levels were significantly decreased. Our results suggest that when the bees were under stress, the brain levels of OA and DA were depressed, and this may have caused latency in the rewarding behavior. The serotonin (5-HT) levels under these stress treatments were not changed.     

Occurrence of the allene oxide cyclase in different organs and tissues of Arabidopsis thaliana

Occurrence of an essential enzyme in jasmonate (JA) biosynthesis, the allene oxide cyclase, (AOC) was analyzed in different developmental stages and various organs of Arabidopsis thaliana plants by immuno blot analysis and immunocytological approaches. Levels of AOC and of the two preceding enzymes in JA biosynthesis increased during seedling development accompanied by increased levels of JA and 12-oxophytodienoic acid levels after 4 and 8 weeks. Most tissues including all vascular bundles and that of flower buds contain AOC protein. Flowers shortly before opening, however, contain AOC protein preferentially in ovules, stigma cells and vascular bundles, whereas in anthers and pollen AOC could not be detected. The putative roles of AOC and JA in development are discussed.     

Tocopherol content in tissues of mammals of different ecogenesis

Concentration of vitamin E (α-tocopherol) was studied in liver, kidney, heart, and skeletal muscle of 12 mammalian species, of which nine inhabited under natural conditions, while three were farm-bred. In the majority of the species the highest tocopherol content has been found as a rule in the animal liver and kidney. Essential differences have been revealed in distribution and level of α-tocopherol including those in taxonomically close species. These differences seem to be due to ecological peculiarities of the animals. The obtained results allow considering α-tocopherol as one of factors of maintenance of the natural antioxidant system at the steady level.     

不同品种苦荞麦不同器官总黄酮含量的比较分析

Total flavonoids content in stems,leaves and seeds of seven cultivars of Fagopyrum tataricum(L.) Gaertn.and a cultivar of F.esculentum Moench were determined by UV-visible spectrophotometry.The results show that total flavonoids content in leaves is higher.Total flavonoids content among different cultivars of F.tataricum has a great difference.Among seven cultivars of F.tataricum,total flavonoids content is the highest in leaves and seeds of ’Jiande Tartary Buckwheat’ with 106.16 and 24.09 mg·g-1,respectively,and in stems of ’Dingbian Tartary Buckwheat’ with 34.65 mg·g-1.The comprehensive analysis result indicates that ’Jiande Tartary Buckwheat’ is the best germplasm resources of F.tataricum and worth to further extension.     

Biogenic amine uptake by nerve cords from the American cockroach and the influence of amidines on amine uptake and release

Uptake of 5-hydroxytryptamine (5-HT) by isolated whole nerve cords of the American cockroach, Periplaneta americana (L.), involved a dual component system, with one component consisting of rapid active uptake and the other of passive diffusion. Using high performance liquid chromatography with electrochemical detection, it was shown that nerve cords contained 5-HT levels of about 350 ng/g and an equivalent amount of 5-hydroxyindoleacetic acid (5-HIAA), a 5-HT metabolite not previously reported in cockroach nerve cords. Amidines had no discernable effect on uptake of 5-HT or octopamine by nerve cords or on endogenous levels of 5-HT and 5-HIAA in cords.