共查询到20条相似文献,搜索用时 31 毫秒
1.
I. Eujayl M. Baum W. Powell W. Erskine E. Pehu 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,97(1-2):83-89
A genetic linkage map of Lens sp. was constructed with 177 markers (89 RAPD, 79 AFLP, six RFLP and three morphological markers) using 86 recombinant inbred
lines (F6:8) obtained from a partially interspecific cross. The map covered 1073 cM of the lentil genome with an average distance of
6.0 cM between adjacent markers. Previously mapped RFLP markers were used as anchor probes. The morphological markers, pod
indehiscence, seed-coat pattern and flower-color loci were mapped. Out of the total linked loci, 8.4% showed segregation distortion.
More than one-fourth of the distorted loci were clustered in one linkage group. AFLP markers showed more segregation distortion
than the RAPD markers. The AFLP and RAPD markers were intermingled and clustering of AFLPs was seldom observed. This is the
most extensive genetic linkage map of lentil to-date. The marker density of this map could be used for the identification
of markers linked to quantitative trait loci in this population.
Received: 6 November 1997 / Accepted: 10 February 1998 相似文献
2.
Towards a saturated sorghum map using RFLP and AFLP markers 总被引:20,自引:3,他引:17
K. Boivin M. Deu J.-F. Rami G. Trouche P. Hamon 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,98(2):320-328
A near-saturated sorghum genetic linkage map was produced using RFLP, AFLP and morphological markers. First a composite,
essentially RFLP-based genetic linkage map was obtained from analyses of two recombinant inbred populations. This map includes
343 loci for 11 linkage groups spanning 1352 cM. Since this map was constructed with many previously mapped heterologous probes,
it offers a good basis for synteny studies. Separately, an AFLP map was obtained from the analysis of 168 bands revealed from
12 primer pair combinations. It includes 137 loci for 11 linkage groups spanning 849 cM. Taking into account the different
data sets, we constructed a combined genetic linkage map including 443 loci spanning 1899 cM. Two main features are to be
noted: (1) the distribution of AFLPs along the genome is not uniform; (2) an important stretching of the former core map is
induced after adding the AFLPs.
Received: 10 May 1998 / Accepted: 13 July 1998 相似文献
3.
L. Gentzbittel E. Mestries S. Mouzeyar F. Mazeyrat S. Badaoui F. Vear D. Tourvieille de Labrouhe P. Nicolas 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,99(1-2):218-234
A map of the sunflower genome, based on expressed sequences and consisting of 273 loci, was constructed. The map incorporates
data from seven F2 populations, for a total of 1115 individuals. Two hundred and fourty five loci corresponding to 170 anonymous cDNA markers
and four loci for morphological markers were mapped. We also mapped 18 loci corresponding to previously described genes or
to sequences obtained through homology cloning. The unit maps vary from 774 cM to 1060 cM, with an average value of 14 major
linkage groups. The integrated map is arranged in 17 major linkage groups including 238 loci, plus four small segments with
2–5 marker loci; and covers 1573 cM with an overall average marker interval of 7 cM. Thirty five percent of the markers were
dominant in nature and 30% showed inter-linkage group duplication without any indication of homoeologous linkage groups. Evidence
is provided for the independence of two distinct fertility restoration genes, for the presence of two loosely linked branching
loci, and for marker tightly linked to the Rf1 restoration locus. This map provides an efficient tool in breeding applications such as disease-resistance mapping, QTL analyses
and marker-assisted selection.
Received: 27 August 1998 / Accepted: 28 December 1998 相似文献
4.
Genetic linkage map in sour cherry using RFLP markers 总被引:6,自引:0,他引:6
D. Wang R. Karle T. S. Brettin A. F. Iezzoni 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,97(8):1217-1224
Restriction fragment length polymorphism (RFLP) linkage maps of two tetraploid sour cherry (Prunus cerasus L., 2n=4x=32) cultivars, Rheinische Schattenmorelle (RS) and Erdi Botermo (EB), were constructed from 86 progeny from the cross RS×EB.
The RS linkage map consists of 126 single-dose restriction fragment (SDRF, Wu et al. 1992) markers assigned to 19 linkage
groups covering 461.6 cM. The EB linkage map has 95 SDRF markers assigned to 16 linkage groups covering 279.2 cM. Fifty three
markers mapped in both parents were used as bridges between both maps and 13 sets of homologous linkage groups were identified.
Homoeologous relationships among the sour cherry linkage groups could not be determined because only 15 probes identified
duplicate loci. Fifty nine of the markers on the linkage maps were detected with probes used in other Prunus genetic linkage maps. Four of the sour cherry linkage groups may be homologous with four of the eight genetic linkage groups
identified in peach and almond. Twenty one fragments expected to segregate in a 1 : 1 ratio segregated in a 2 : 1 ratio. Three
of these fragments were used in the final map construction because they all mapped to the same linkage group. Six fragments
exhibited segregation consistent with the expectations of intergenomic pairing and/or recombination.
Received: 1 April 1998 / Accepted: 9 June 1998 相似文献
5.
K. Richter J. Schondelmaier C. Jung 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,97(8):1225-1234
Resistance loci for seedling-stage resistance to net blotch disease (Drechslera teres) in barley were mapped with molecular markers in an F2 population derived from a cross between the susceptible barley cultivar ‘Arena’ and the resistant Ethiopian landrace ‘Hor
9088’. Disease reactions were scored with first and second leaves of 2-week-old plants 7 and 9 days after inoculation with
a single spore-derived isolate. For linkage analysis, 22 RFLP markers and 284 AFLP markers were used. The seven linkage groups
covered 1153.3 cM with an average marker interval of 3.76 cM. The resistance was determined to be inherited in a quantitative
manner. Altogether, 12 QTLs were mapped with positions depending on the leaf used for testing and the time period after infection.
Heritability in the broad sense ranged between 0.21 and 0.37.
Received: 26 May 1998 / Accepted: 9 June 1998 相似文献
6.
C. Taylor K. W. Shepherd P. Langridge 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,97(5-6):1000-1012
A genetic map of the long arm of chromosome 6R of rye was constructed using eight homoeologous group-6 RFLP clones and five
PCR markers derived from the rye-specific dispersed repetitive DNA family, R173. The map was developed using a novel test-cross
F1 (TC-F1) population segregating for resistance to the cereal cyst nematode. Comparisons were made between the map generated with
other rye and wheat group-6 chromosome maps by the inclusion of RFLP clones previously mapped in those species. Co-linearity
was observed for common loci. This comparison confirmed a dramatic reduction in recombination for chromosome 6R in the TC-F1 population. The CreR locus was included in the linkage map via progeny testing of informative TC-F1 individuals. CreR mapped 3.7 cM distal from the RFLP locus, XksuF37. Comparative mapping should allow the identification of additional RFLP markers more closely linked to the CreR locus.
Received: 14 April 1998 / Accepted: 29 April 1998 相似文献
7.
A genetic linkage map of durum wheat 总被引:20,自引:6,他引:14
A. Blanco M. P. Bellomo A. Cenci C. De Giovanni R. D’Ovidio E. Iacono B. Laddomada M. A. Pagnotta E. Porceddu A. Sciancalepore R. Simeone O. A. Tanzarella 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,97(5-6):721-728
A genetic linkage map of tetraploid wheat [Triticum turgidum (L.) Thell.] was constructed using segregation data from a population of 65 recombinant inbred lines (RILs) derived from
a cross between the durum wheat cultivar Messapia and accession MG4343 of T. turgidum (L.) Thell. ssp dicoccoides (Korn.) Thell. A total of 259 loci were analysed, including 244 restriction fragment length polymorphisms (RFLPs), one PCR
(polymerase chain reaction) marker (a sequence coding for a LMW (low-molecular-weight) glutenin subunit gene located at the
Glu-B3 locus), seven biochemical (six seed-storage protein loci and one isozyme locus) and seven morphological markers. A total
of 213 loci were mapped at a LOD≥3 on all 14 chromosomes of the A and B genomes. The total length of the map is 1352 cM and
the average distance between adjacent markers is 6.3 cM. Forty six loci could not be mapped at a LOD≥3. A fraction (18.6%)
of the markers deviated significantly from the expected Mendelian ratios; clusters of loci showing distorted segregation were
found on chromosomes 1B, 3AL, 4AL, 6AL and 7AL. The durum wheat map was compared with the published maps of bread wheat using
several common RFLP markers and general features are discussed. The markers detected the known structural rearrangements involving
chromosomes 4A, 5A and 7B as well as the translocation between 2B-6B, but not the deletion on 2BS. This map provides a useful
tool for analysing and breeding economically important quantitative traits and for marker-assisted selection, as well as for
studies of genome organisation in small grain cereal species.
Received: 5 January 1998 / Accepted: 31 March 1998 相似文献
8.
B. C. Kang S. H. Nahm J. H. Huh H. S. Yoo J. W. Yu M. H. Lee B.-D. Kim 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2001,102(4):531-539
We have constructed a molecular linkage map of pepper (Capsicum spp.) in an interspecific F2 population of 107 plants with 150 RFLP and 430 AFLP markers. The resulting linkage map consists of 11 large (206–60.3 cM)
and 5 small (32.6–10.3 cM) linkage groups covering 1,320 cM with an average map distance between framework markers of 7.5
cM. Most (80%) of the RFLP markers were pepper-derived clones, and these markers were evenly distributed across the genome.
By using 30 primer combinations, we were able to generate 444 AFLP markers in the F2 population. The majority of the AFLP markers clustered in each linkage group, although PstI/MseI markers were more evenly distributed than EcoRI/MseI markers within the linkage groups. Genes for the biosynthesis of carotenoids and capsaicinoids were mapped on our linkage
map. This map will provide the basis of studying secondary metabolites in pepper.
Received: 20 October 1999 / Accepted: 3 July 2000 相似文献
9.
Identification of quantitative trait loci contributing to Fusarium wilt resistance on an AFLP linkage map of flax (Linum usitatissimum) 总被引:3,自引:0,他引:3
W. Spielmeyer A. G. Green D. Bittisnich N. Mendham E. S. Lagudah 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,97(4):633-641
An AFLP genetic linkage map of flax (Linum usitatissimum) was used to identify two quantitative trait loci (QTLs) on independent linkage groups with a major effect on resistance
to Fusarium wilt, a serious disease caused by the soil pathogen Fusarium oxysporum (lini). The linkage map was constructed using a mapping population from doubled-haploid (DH) lines. The DH lines were derived
from the haploid component of F2 haploid-diploid twin seed originating from a cross between a polyembryonic, low-linolenic-acid genotype (CRZY8/RA91) and
the Australian cultivar ‘Glenelg’. The AFLP technique was employed to generate 213 marker loci covering approximately 1400 cM
of the flax genome (n=15) with an average spacing of 10 cM and comprising 18 linkage groups. Sixty AFLP markers (28%) deviated
significantly (P<0.05) from the expected segregation ratio. The map incorporated RFLP markers tightly linked to flax rust (Melamspora lini) resistance genes and markers detected by disease resistance gene-like sequences. The study illustrates the potential of
the AFLP technique as a robust and rapid method to generate moderately saturated linkage maps, thereby allowing the molecular
analysis of traits, such as resistance to Fusarium wilt, that show oligogenic patterns of inheritance.
Received: 8 December 1997 / Accepted: 7 April 1998 相似文献
10.
Ulloa M Meredith WR Shappley ZW Kahler AL 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2002,104(2-3):200-208
An RFLP genetic linkage joinmap was constructed from four different mapping populations of cotton (Gossypium hirsutum L.). Genetic maps from two of the four populations have been previously reported. The third genetic map was constructed from
199 bulk-sampled plots of an F2.3 (HQ95–6×’MD51ne’) population. The map comprises 83 loci mapped to 24 linkage groups with an average distance between markers
of 10.0 centiMorgan (cM), covering 830.1 cM or approximately 18% of the genome. The fourth genetic map was developed from
155 bulk-sampled plots of an F2.3 (119– 5 sub-okra×’MD51ne’) population. This map comprises 56 loci mapped to 16 linkage groups with an average distance between
markers of 9.3 cM, covering 520.4 cM or approximately 11% of the cotton genome. A core of 104 cDNA probes was shared between
populations, yielding 111 RFLP loci. The constructed genetic linkage joinmap from the above four populations comprises 284
loci mapped to 47 linkage groups with the average distance between markers of 5.3 cM, covering 1,502.6 cM or approximately
31% of the total recombinational length of the cotton genome. The linkage groups contained from 2 to 54 loci each and ranged
in distance from 1.0 to 142.6 cM. The joinmap provided further knowledge of competitive chromosome arrangement, parental relationships,
gene order, and increased the potential to map genes for the improvement of the cotton crop. This is the first genetic linkage
joinmap assembled in G. hirsutum with a core of RFLP markers assayed on different genetic backgrounds of cotton populations (Acala, Delta, and Texas plain).
Research is ongoing for the identification of quantitative trait loci for agronomic, physiological and fiber quality traits
on these maps, and the identification of RFLP loci lineage for G. hirsutum from its diploid progenitors (the A and D genomes).
Received: 23 February 2001 / Accepted: 8 June 2001 相似文献
11.
12.
S. Fukuoka H. Namai K. Okuno 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,97(3):446-449
Complementary recessive genes hwd1 and hwd2 controlling hybrid breakdown (weakness of F2 and later generations) were mapped in rice using RFLP markers. These genes produce a plant that is shorter and has fewer
tillers than normal plants when the two loci have only one or no dominant allele at both loci. A cultivar with two dominant
alleles at the hwd1 locus and a cultivar with two dominant alleles at the hwd2 locus were crossed with a double recessive tester line. Linkage analysis was carried out for each gene independently in two
F2 populations derived from these crosses. hwd1 was mapped on the distal region of rice genetic linkage map for chromosome 10, flanked by RFLP markers C701 and R2309 at
a distance of 0.9 centiMorgans (cM) and 0.6 cM, respectively. hwd2 was mapped in the central region of rice genetic linkage map for chromosome 7, tightly linked with 4 RFLP markers without
detectable recombination. The usefulness of RFLP mapping and map information for the genes controlling reproductive barriers
are discussed in the context of breeding using diverse rice germplasm, especially gene introduction by marker-aided selection. 相似文献
13.
R. Freyre P. W. Skroch V. Geffroy A.-F. Adam-Blondon A. Shirmohamadali W. C. Johnson V. Llaca R. O. Nodari P. A. Pereira S.-M. Tsai J. Tohme M. Dron J. Nienhuis C. E. Vallejos P. Gepts 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,97(5-6):847-856
Three RFLP maps, as well as several RAPD maps have been developed in common bean (Phaseolus vulgaris L.). In order to align these maps, a core linkage map was established in the recombinant inbred population BAT93×Jalo EEP558
(BJ). This map has a total length of 1226 cM and comprises 563 markers, including some 120 RFLP and 430 RAPD markers, in addition
to a few isozyme and phenotypic marker loci. Among the RFLPs mapped were markers from the University of California, Davis
(established in the F2 of the BJ cross), University of Paris-Orsay, and University of Florida maps. These shared markers allowed us to establish
a correspondence between the linkage groups of these three RFLP linkage maps. In total, the general map location (i.e., the
linkage group membership and approximate location within linkage groups) has been determined for some 1070 markers. Approaches
to align this core map with other current or future maps are discussed.
Received: 10 March 1998 / Accepted: 22 April 1998 相似文献
14.
A genetic map of Asparagus officinalis based on integrated RFLP, RAPD and AFLP molecular markers 总被引:6,自引:0,他引:6
A. Spada E. Caporali G. Marziani P. Portaluppi F. M. Restivo F. Tassi A. Falavigna 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,97(7):1083-1089
An integrated genetic map of the dioecious species Asparagus officinalis L. has been constructed on the basis of RFLP, RAPD, AFLP and isoenzyme markers. The segregation analysis of the polymorphic
markers was carried out on the progeny of five different crosses between male and female doubled-haploid clones generated
by anther culture. A total of 274 markers have been organized to ten linkage groups spanning 721.4 cM. Since the haploid chromosome
number of asparagus is ten, the established linkage groups probably represent the different chromosomes; however, the only
group associated with a specific chromosome is the one which includes sex, whose determinant genes have been located on chromosome
5. A total of 33 molecular markers (13 RFLPs, 18 AFLPs, 2 RAPDs and 1 isoenzyme) have been located on this chromosome. The
closest marker to the sex determinant is the AFLP SV marker at 3.2 cM.
Received: 26 March 1998 / Accepted: 30 April 1998 相似文献
15.
F. Perez A. Menendez P. Dehal C. F. Quiros 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,98(8):1183-1193
A genetic map was constructed from an F2 population of 76 individuals for the purpose of comparing the arrangement of loci in the A and E Solanum genomes. This progeny was derived from an interspecific cross between the species Solanum palustre×Solanum etuberosum, both of which are E-genome species. Two hundred and eighty one probes previously mapped in tomato and potato (A-genome,
as postulated for diploid cultivated potato species by Matsubayashi 1991) disclosed 109 segregating loci in this population.
Of these, 80 loci were linked in 19 linkage groups covering a total of 720.4 cM, with an average of 9 cM between markers.
Although the genetic map of the E-genome showed conservation for most linkage groups with those of tomato and the A-genome,
various translocations and possible inversions and transpositions were detected. It is evident that the accumulation of these
structural changes in the E-genome is sufficient to cause the observed hybrid sterility. The major rearrangements in the E-genome
included multiple translocations involving mosly linkage groups 2 and 8. Also a transposition was detected on group 9, with
the same group-10 inversion distinguishing potato from tomato. Definitively groups 2, 8, 9 and 10, and possibly groups 1,
4 and 12, in the E-genome are structurally different from their homologues in the A-genome. In general, recombination values
were larger in the E- than in the A-genome. The extensive structural differentiation of the E-genome with respect to that
of potato and tomato justifies its present designation as a different genome, which is supported by previous chromosome-pairing
studies. The difficult introgression of desirable traits from the Etuberosum species into potato can be explained by these structural differences.
Received: 1 February 1998 / Accepted: 8 October 1998 相似文献
16.
《Genomics》1995,29(3)
Three sets of linkage maps (index, comprehensive microsatellite, and unified) have been constructed for human chromosome 14 based on genotypes from the CEPH reference pedigrees. The index maps consist of 18 microsatellite markers, with heterozygosities of at least 68% and intermarker spacing no greater than 11 cM. The sex-average comprehensive microsatellite map is 125 cM in length and includes 115 markers with 54 loci uniquely placed with odds for marker order of at least 1000:1. The sex-average index map length is 121 cM, and the female- and male-specific maps are 143 and 101 cM, respectively. A unified map was also constructed from 147 loci (162 marker systems), which includes 32 RFLP markers in addition to the 115 microsatellites. The sex-average length of the unified map is 128 cM with 69 loci uniquely placed. Our maps are anchored by a microsatellite telomere marker sCAW1 (D14S826), developed from a telomere YAC clone TYAC196, which extends the linkage map to the physical terminus of the long arm of chromosome 14. Furthermore, we have also physically mapped seven of the loci by fluorescencein situhybridization of cosmid clones orAlu-PCR products amplified from YACs containing the marker sequences. Together with previously established cytogenetic map designations for other loci, our maps display links between genetic markers for 10 of 13 cytogenetic bands of chromosome 14 at the 550 genome band resolution. 相似文献
17.
A genetic linkage map of cowpea (Vigna unguiculata) developed from a cross between two inbred, domesticated lines 总被引:1,自引:0,他引:1
C. M. Menéndez A. E. Hall P. Gepts 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1997,95(8):1210-1217
We have constructed a genetic linkage map within the cultivated gene pool of cowpea (2n=2x=22) from an F8 recombinant inbred population (94 individuals) derived from a cross between the inbreds IT84S-2049 and 524B. These breeding
lines, developed in Nigeria and California, show contrasting reactions against several pests and diseases and differ in several
morphological traits. Parental lines were screened with 332 random RAPD decamers, 74 RFLP probes (bean, cowpea and mung bean
genomic DNA clones), and 17 AFLP primer combinations. RAPD primers were twice as efficient as AFLP primers and RFLP probes
in detecting polymorphisms in this cross. The map consists of 181 loci, comprising 133 RAPDs, 19 RFLPs, 25 AFLPs, three morphological/classical
markers, and a biochemical marker (dehydrin). These markers identified 12 linkage groups spanning 972 cM with an average distance
of 6.4 cM between markers. Linkage groups ranged from 3 to 257 cM in length and included from 2 to 41 markers, respectively.
A gene for earliness was mapped on linkage group 2. Seed weight showed a significant association with a RAPD marker on linkage
group 5. This map should facilitate the identification of markers that “tag” genes for pest and disease resistance and other
traits in the cultivated gene pool of cowpea.
Received: 16 September 1996 / Accepted: 25 April 1997 相似文献
18.
V. Laucou K. Haurogné N. Ellis C. Rameau 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,97(5-6):905-915
A genetic linkage map of Pisum sativum L. was constructed based primarily on RAPD markers that were carefully selected for their reproducibility and scored in a
population of 139 recombinant inbred lines (RILs). The mapping population was derived from a cross between a protein-rich
dry-seed cultivar ‘Térèse’ and an increased branching mutant (K586) obtained from the pea cultivar ‘Torsdag’. The map currently
comprises nine linkage groups with two groups comprising only 6 markers (n=7 in pea) and covers 1139 cM. This RAPD-based map has been aligned with the map based on the (JI281×JI399) RILs population
that currently includes 355 markers in seven linkage groups covering 1881 cM. The difference in map lengths is discussed.
For this alignment 7 RFLPs, 23 RAPD markers, the morphological marker le and the PCR marker corresponding to the gene Uni were used as common markers and scored in both populations.
Received: 13 March 1998 / Accepted: 29 April 1998 相似文献
19.
V. Laporte D. Merdinoglu P. Saumitou-Laprade G. Butterlin P. Vernet J. Cuguen 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,96(6-7):989-996
The present study shows that the recently described mitochondrial H haplotype is associated with cytoplasmic male-sterility
(CMS). This new source of CMS appears to be different from the mitotype E-associated CMS most frequently found in natural
populations. A mitotype H progeny with a sexual phenotype segregation was used to identify a gene restoring male fertility
(R1H ). Using bulk segregant analysis (BSA), nine RAPD markers linked to this restorer locus were detected and mapped. The comparison
with other Beta genetic maps shows that the closest RAPD marker, distant from R1H by 5.2 cM, belongs to the same linkage group as the monogermy locus. In order to determine the position of R1H more precisely, four RFLP loci within this linkage group were mapped in the segregating progeny. It thus became possible
to construct a linkage map of the region containing the RFLP, RAPD and R1H loci. The closest RFLP marker was located 1.7 cM away from R1H. However, a nuclear gene restoring the ‘Owen’ CMS which is currently used in sugar beet breeding is reportedly linked to
the monogermy locus, raising the question of a possible identity between the new CMS system and the ‘Owen’ CMS.
Received: 15 September 1997 / Accepted: 1 December 1997 相似文献
20.
A linkage map of the pea (Pisum sativum L.) genome containing cloned sequences of known function and expressed sequence tags (ESTs) 总被引:3,自引:0,他引:3
B. J. Gilpin J. A. McCallum T. J. Frew G. M. Timmerman-Vaughan 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1997,95(8):1289-1299
A linkage map of the pea (Pisum sativum L.) genome is presented which is based on F2 plants produced by crossing the marrowfat cultivar ‘Primo’ and the blue-pea breeding line ‘OSU442-15’. This linkage map consists
of 209 markers and covers 1330 cM (Kosambi units) and includes RFLP, RAPD and AFLP markers. By mapping a number of anchor
loci, the ‘Primo’בOSU442-15’ map has been related to other pea linkage maps. A feature of the map is the incorporation of
29 loci representing genes of known function, obtained from other laboratories. The map also contains RFLP loci detected using
sequence-characterized cDNA clones developed in our laboratory. The putative identities of 38 of these cDNA clones were assigned
by examining public-sequence databases for protein or nucleotide-sequence similarities. The conversion of sequence-characterized
pea cDNAs into PCR-amplifiable and polymorphic sequence-tagged sites (STSs) was investigated using 18 pairs of primers designed
for single-copy sequences. Eleven polymorphic STSs were developed.
Received: 18 June 1997 / Accepted: 11 August 1997 相似文献