Robert Earle Buchanan: an unappreciated scientist

Robert Earle Buchanan (1883-1973), 19th President of the Society of American Bacteriologists (later American Society for Microbiology), was one of the more important 20th century microbiologists. He was a dominant force in creating the field of bacterial systematics and made significant contributions to microbial physiology. He also numbered a number of influential textbooks. A reasonable conclusion is that Buchanan was a major cultivator of modern microbiology. To justify that assertion, I have four major objectives in this essay: i) a brief biographical review of Buchanan's early life; ii) a brief review of his scientific contributions, many of which go beyond his recognized contributions to bacterial systematics; iii) Buchanan was an important academic administrator who created the microbiology program and fostered a strong graduate education program at Iowa State, iv)finally, I close the essay with a focus on Buchanan's "moral character."     

Alice C. Evans: breaking barriers

Despite severe and persistent criticism of her research, Alice Evans persevered in her pioneering work on the bacterial contamination of milk, identifying the organism that caused undulant fever and demonstrating that drinking unpasteurized cow's milk could transmit the disease, undulant fever, to humans. The opprobrium that Alice Evans endured was unrelenting, even after her election as the first President of the Society of American Bacteriologists, (now the American Society for Microbiology), but she remained undeterred, a true heroine of American microbiology and a magnificent public health worker.     

The Society for Applied Bacteriology The First Fifty Years

The major part of this history, the formative years, was compiled by the Society's Archivist entirely from original materials. As such it complements an earlier account of the Society's development (S.B. Thomas 1963 Journal of Applied Bacteriology 26 , 66–68). The other part of this article, the recent past, was compiled by the Honorary General Secretary from the minutes of the last 10 years of committee meetings. This bipartite approach gives emphasis to those features that have had a persistent influence on the evolution of the Society , the needs and interests of young microbiologists, an awareness that publications and meetings have to be tailored to meet the needs and aspirations of the membership, a respect for those who have given much to the Society, and a perception of trends, both national and international, in the development of applied bacteriology within the broader context of microbiology. The names of those who are or have been honorary officers and honorary members of the Society are given in the Appendix.     

The American Society for Neurochemistry (ASN): Antecedents, Founding, and Early Years

Events leading to and the influences on the founding of the American Society for Neurochemistry are recounted, with emphasis on early activities of neurochemists in the United States, as well as the international activities, that led to the founding of both the International and American societies (in 1965 and 1969, respectively). The founding of the American Society for Neurochemistry in the period 1968-1969 and its first annual meeting in 1970 are described, together with significant developments during the early years of the Society.     

The 21st SEM National Congress (Seville, 17-20 September, 2007).

Almost 700 scientists met in the historic city of Seville, September 17-20, 2007, to attend the 21st National Congress of the Spanish Society for Microbiology (SEM). The attendees--from Spain and other European and American countries--had an opportunity to share the newest discoveries in their fields of research and to learn of the progress being made in numerous areas of microbiology through 64 symposia lectures, 1 workshop, 50 oral communications, and more than 400 posters.     

16S rRNA gene-based oligonucleotide microarray for environmental monitoring of the betaproteobacterial order "Rhodocyclales"

For simultaneous identification of members of the betaproteobacterial order "Rhodocyclales" in environmental samples, a 16S rRNA gene-targeted oligonucleotide microarray (RHC-PhyloChip) consisting of 79 probes was developed. Probe design was based on phylogenetic analysis of available 16S rRNA sequences from all cultured and as yet uncultured members of the "Rhodocyclales." The multiple nested probe set was evaluated for microarray hybridization with 16S rRNA gene PCR amplicons from 29 reference organisms. Subsequently, the RHC-PhyloChip was successfully used for cultivation-independent "Rhodocyclales" diversity analysis in activated sludge from an industrial wastewater treatment plant. The implementation of a newly designed "Rhodocyclales"-selective PCR amplification system prior to microarray hybridization greatly enhanced the sensitivity of the RHC-PhyloChip and thus enabled the detection of "Rhodocyclales" populations with relative abundances of less than 1% of all bacteria (as determined by fluorescence in situ hybridization) in the activated sludge. The presence of as yet uncultured Zoogloea-, Ferribacterium/Dechloromonas-, and Sterolibacterium-related bacteria in the industrial activated sludge, as indicated by the RHC-PhyloChip analysis, was confirmed by retrieval of their 16S rRNA gene sequences and subsequent phylogenetic analysis, demonstrating the suitability of the RHC-PhyloChip as a novel monitoring tool for environmental microbiology.     

Hans Zinsser: a tale of two cultures

Hans Zinsser, president of the Society of American Bacteriologists in 1926, was known as much for his literary and textbook writing as for his scientific contributions. He was a widely known scientist and person of letters. His early interests in poetry and other forms of literature were maintained and developed during his career as a microbiologist, and his most enduring legacy is based on his writing about microbiology for a general readership as well as his reflective and philosophical autobiography.     

What initiates actin polymerization?

For those working on the actin cytoskeleton, a major theme of the 39th annual meeting of the American Society for Cell Biology [] (Washington DC, December 11-15, 1999) was the elucidation of how actin polymerization is initiated. The emphasis was on the regulation and localization of the Arp2/3 complex, which over the last two years has been shown to stimulate actin nucleation, and on the identification of additional proteins that interact with actin and Arp2/3 in a variety of organisms.     

实施“三高”教学,创建“微生物学实验”课程新体系

深化微生物实验教学改革,实施“高起点、高效率、高质量”教学,从调整教学内容,改革教学方法,建立和完善考评机制入手,创建“以基础知识和基本技能为基础性内容,以创新能力培养为核心内容”的微生物学实验课程新体系,激发了学生对微生物学实验的兴趣,培养了学生独立思考、创新思维和综合动手的能力。     

Dissin' "the Standard": Ebonics as Guerrilla Warfare at Capital High

This article analyzes the discourse styles, including the linguistic practices, of a group of African American high school students and offers a twofold conclusion: (1) Ebonics or Black English is the norm against which all other speech practices are evaluated by the students at the research site and (2) "the standard"—that is, the standard English dialect—is constructed as a vernacular. As a vernacular, this discourse is not privileged; indeed, it is "dissed" (disrespected) and is only "leased" by the students on a daily basis from nine to three. This linguistic practice is centrally implicated in the postulated guerrilla warfare at the school. With data from a predominantly African American high school in Washington, D.C., the effects of this practice on African American academic achievement are documented. Several policy implications are also noted.     

Genetic evidence of "American" and "European" type symbiotic algae of Paramecium bursaria Ehrenberg

Paramecium bursaria is composed of a "host" ciliate and a "symbiont" green alga. Based upon physiology, DNA hybridization and virus infection, two types of symbionts, called "American" type and "European" type, have been reported to date. Here, we determined the 18S rDNA and internal transcribed spacer 2 (ITS2) regions for both "American" and "European" types. Sequence features clearly separated into two lineages; NC64A (USA), Syngen 2-3 (USA), Cs2 (Chinese), MRBG1 (Australian), and Japanese strains belong to the "American", whereas PB-SW1 (German) and CCAP 1660/11 (British) strains belong to the "European". In "American" 18S rDNA, three introns were inserted in the same positions as for previously described Japanese symbionts. In "European" 18S rDNA, a single intron occurred in a different position than in the "American". Between the types, sequence differences were seven or eight nucleotides (0.39 %) in the 18S rDNA exon, and more than 48 nucleotides (19.2 %) in ITS2 regions. We subsequently sequenced the host 18S rDNA. As a result, two groups: Cs2, MRBG1, and Japanese strains, and PB-SW1 and CCAP 1660/11 strains, were separated (with 23 substitutions and 4 insertions or deletions between the groups). The congruent separations between hosts and symbionts may imply that the type of symbiont depends on the host type.     

Practice advisory on liposuction

COMMITTEE STATEMENT: At the 69th annual meeting of the American Society of Plastic Surgeons (ASPS) in October of 2000, the ASPS Board of Directors convened the Task Force on Patient Safety in Office-Based Surgery Facilities. The task force was assembled in the wake of several highly publicized patient deaths involving plastic surgery and increasing state legislative and regulatory activity of office-based surgery facilities. In response to the increased scrutiny of the office-based surgery setting, the task force produced two practice advisories: "Procedures in the Office-Based Surgery Setting" and "Patient Selection in the Office-Based Surgery Setting." Since the task force's inception, professional and public awareness of patient safety issues has continued to grow. This heightened interest resulted in an increased need for plastic surgeons to communicate their views on the topic. To meet this challenge, the task force evolved into the Committee on Patient Safety, allowing the committee to address topics affecting the safety and welfare of plastic surgery patients, regardless of the facility setting. The "Practice Advisory on Liposuction" is the first advisory developed since the committee was formed. It was a lengthy and painstaking process for the committee, which included representatives from related plastic surgery organizations as well as the American Society of Anesthesiologists (ASA). Committee members included Ronald E. Iverson, M.D., chair; Jeffery L. Apfelbaum, M.D., ASA representative; Bruce L. Cunningham, M.D., ASPS/Plastic Surgery Educational Foundation (PSEF) Joint Outcomes Task Force representative; Richard A. D'Amico, M.D., ASPS representative; Victor L. Lewis, Jr., M.D., ASPS Health Policy Analysis Committee representative; Dennis J. Lynch, M.D., ASPS representative; Noel B. McDevitt, M.D., ASPS Deep Vein Thrombosis Task Force representative; Michael F. McGuire, M.D., The American Society for Aesthetic Plastic Surgery (ASAPS) representative; Louis Morales, Jr., M.D., American Society of Maxillofacial Surgeons representative; Calvin R. Peters, M.D., Florida Ad Hoc Commission on Patient Safety representative; Robert Singer, M.D., American Association for Accreditation of Ambulatory Surgery Facilities representative; Thomas Ray Stevenson, M.D., American College of Surgeons representative; Rebecca S. Twersky, M.D., ASA representative; Ronald H. Wender, M.D., ASA representative; and James A. Yates, ASAPS representative. The authors thank members of the committee for the insights they brought to this process. The final document represents their significant contributions to these efforts. They would also like to recognize DeLaine Schmitz and Pat Farrell of the ASPS staff for their work on and support of this project.     

Current topics for teaching skeletal muscle physiology

Contractions of skeletal muscles provide the stability and power for all body movements. Consequently, any impairment in skeletal muscle function results in some degree of instability or immobility. Factors that influence skeletal muscle structure and function are therefore of great interest both scientifically and clinically. Injury, disease, and old age are among the factors that commonly contribute to impairment in skeletal muscle function. The goal of this article is to update current concepts of skeletal muscle physiology. Particular emphasis is placed on mechanisms of injury, repair, and adaptation in skeletal muscle as well as mechanisms underlying the declining skeletal muscle structure and function associated with aging. For additional materials please refer to the "Skeletal Muscle Physiology" presentation located on the American Physiological Society Archive of Teaching Resources Web site (http://www.apsarchive.org).     

2009 Society for Medical Anthropology Conference: Changing Anthropology,Changing Society

Fifty years after the founding of the field of medical anthropology, the Society for Medical Anthropology of the American Anthropological Association held its first independent meeting on September 24-27, 2009, at Yale University.Fifty years after the founding of the field of medical anthropology, the Society for Medical Anthropology of the American Anthropological Association held its first independent meeting on September 24-27, 2009, at Yale University in New Haven, Connecticut. The conference, Medical Anthropology at the Intersections, drew an international audience of more than 1,000 scholars.In her opening remarks, program Chair Marcia Inhorn noted that medical anthropology has been interdisciplinary since its inception. This assertion was supported at a roundtable discussion, Founding Medical Anthropology and the Society for Medical Anthropology, which featured four of the field’s founders.Asked to identify the factors that led to the development of medical anthropology, the panelists emphasized the role of changes in the practice and landscape of medicine in the late 1950s and early 1960s in the United States. According to Hazel Weidman, who helped spearhead the Society for Medical Anthropology, medical personnel sought social scientists’ guidance in the new clinical environments created by the increasing involvement of U.S. physicians in global development work and by the community-oriented approach to mental health encouraged by the Community Mental Health Act of 1963. The novel inclusion of lifestyle as a determinant of health at this time also played a role, according to Clifford Barnett. Norman Scotch, author of a 1963 review that had helped define medical anthropology as a field, noted that physicians at the time were very interested in the possible applications of the social sciences to medicine [1,2]. Joan Ablon recalled that this emphasis on application led some academic anthropologists to dismiss the medical anthropologist as a “handmaiden to the doctors.” Despite such resistance, interest in medical anthropology as a sub-field was clearly growing among anthropologists. When Weidman helped organize the first gathering of medical anthropologists at an anthropology conference in 1967, attendance was twice what was expected. Panel organizer Alan Harwood noted that the Society for Medical Anthropology transformed its newsletter into a professional journal, Medical Anthropology Quarterly, in 1983. According to Inhorn, the society has 1,300 members today.For the panelists, medical anthropology’s potential for application makes it a compelling scholarly pursuit. As Barnett stated in explaining his decision to work in anthropology: “If you know how a society works, you can change it.”     

Presidential address 2002. Parasitology in the 21st century

During the past several weeks, I, like many of my predecessors, have read many speeches previously delivered at the annual meeting of the American Society of Parasitologists (ASP). These are wonderful, exciting papers, and I recommend them to you. The history of our Society and much of parasitological research and philosophy are contained in these speeches. An understanding of parasitology and specifically of the ASP in the 21st century necessitates an understanding of our roots. Just as proteomics, genomics, DNA, and Mendel are a continuum, so is our progression as a science and as a Society. This thought is not original with me. Several presidents have discussed this point in detail: Eloise Cram in 1956, "Stepping Stones in the History of the American Society of Parasitologists" (Cram, 1956); Martin Ulmer in 1978, "What's Past is Prologue" (Ulmer, 1978); Harry Hoogstraal in 1984, "ASP: Its Historic Role and Modern Opportunities" (Hoogstraal, 1985); and Mike Kemp in 1988, "Parasitology a Degenerate Discipline, Populated by Degenerate Scientists, Studying Degenerate Organisms?" (Kemp, 1989).     

Development of soil microbiology methods: from respirometry to molecular approaches

This review deals with techniques and methods used in the study of the function and development of microorganisms occurring in soil with emphasis on the contributions of Czech Academician Ivan Málek and his coworkers or fellows (Jiří Macura, František Kunc) to the development of basic techniques used in soil microbiology. Early studies, including batch cultivation and respirometric techniques, as well as later developments of percolation and continuous-flow methods of cultivation of soil microorganisms are discussed. Recent developments in the application of analytical chemistry (HPLC or GC) and of molecular biological techniques to ecological questions that have revolutionized concepts in soil microbiology and microbial ecology are also briefly mentioned, including denaturing gradient gel electrophoresis (DGGE), temperature gradient gel electrophoresis (TGGE), phospholipid fatty acid analysis (PLFA) and others. The shift of soil microbiology from the study of individual microorganisms to entire microbial communities, including nonculturable species, is briefly discussed.     

Ubiquitin branches out

In recent years, there has been a marked expansion of recognized roles for ubiquitin (Ub) in processes other than proteasome-dependent proteolysis, as well as a proliferation of Ub-like proteins that also function through covalent attachment to other proteins. The full diversity of functions for these proteins was on display at the American Society for Cell Biology (ASCB) meeting on "Non-Traditional Functions of Ubiquitin and Ubiquitin-Like Proteins" held at Colorado College in Colorado Springs, Colorado (August 11-14th, 2002).     

Value of recN sequences for species identification and as a phylogenetic marker within the family "Leuconostocaceae"

The genera Leuconostoc, Oenococcus, and Weissella (family "Leuconostocaceae") constitute a group of lactic acid bacteria of great interest in food microbiology. From the taxonomic point of view, they are considered phylogenetically coherent according to their 16S rRNA gene sequences and other macromolecules. These three genera were the focus of the present study; specifically, the resolution and discriminatory power of recN (encoding a DNA repair and genetic recombination protein) as a molecular marker at the species level were investigated. For this purpose, partial sequences (about 1200 nt) were obtained from 23 type strains and from several additional strains following direct amplification of recN and subsequent sequencing. Phylogeny was evaluated according to different treeing methods (neighbor joining, maximum likelihood, and maximum parsimony) and the inclusion of variability filters. The results showed that recN, used either alone or in combination with 16S rRNA data, can serve as a phylogenetic marker as well as a tool for species identification.     

Initiating and promoting the aquatic ecosystem health concept: the Society and the Journal

Since the 1970's the management of aquatic habitats has changed from piecemeal monitoring to the ecosystem approach; this was initiated in the North American Great Lakes, comprising social, economic, and environmental aspects. The information included in this paper is based on the presentation made at the Seminar On Ecosystem Approach To Water Management held in Oslo, Norway during 1991. Recently, the multidisciplinary, holistic, and integrated concept of ecosystem health has emerged, and is being advanced for the implementation of an ecosystem approach to environmental management, which has resulted in the formation of an international society (Aquatic Ecosystem Health & Management Society) and the publication of a primary journal (Journal of Aquatic Ecosystem Health). The information has been updated to incorporate new developments and recent progress about the Society and the journal since the Oslo Seminar.     

A Conversation About Health Care Reform

Professor Victor R. Fuchs is the Henry J. Kaiser Jr Professor at Stanford (California) University, where he applies economic analysis to social problems of national concern, with special emphasis on health and medical care. He holds joint appointments in the Economics Department and the School of Medicine''s Department of Health Research and Policy. Professor Fuchs is a Distinguished Fellow of the American Economic Association and a member of the American Philosophical Society, the American Academy of Arts and Sciences, and the Institute of Medicine of the National Academy of Sciences. He was the first economist to receive the Distinguished Investigator Award of the Association for Health Services Research and has also received the Baxter Foundation Health Services Research Prize. Professor Fuchs is president-elect of the American Economic Association. His latest book, The Future of Health Policy, was published by Harvard University Press in 1993.The following edited conversation between Professor Fuchs and Linda Hawes Clever, MD, Editor of the journal, took place on April 8, 1994.