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1.
Synaptonemal complexes of spermatocytes I obtained from C57BL/6j male mice treated with inactivated bacterial vaccines were spread over the hypotonic phase and then were investigated using light microscope. The slides of synaptonemal complexes of mice treated with cyclophosphamide were used as positive control. It is shown possible in principle to reveal synaptonemal complex abnormalities by means of light microscopy. These abnormalities were not more frequent in vaccinated animals than in intact ones. Cyclophosphamide at doses of 100-200 mg/kg induced synaptonemal complex damage practically in 100% of cells 96 hours after the injection. 相似文献
2.
L. D. Safronova E. G. Sergeev M. A. Monakhova 《Moscow University Biological Sciences Bulletin》2012,67(3-4):107-110
We present an electronic microscopy (EM) analysis of synaptonemal complexes (SC) spermatocytes of male silver fox Vulpes fulvus at the pachytene stage. The SC-karyotypes of pachetene cells were made and described. Knowledge of normal SC-karyotype is necessary to reveal synaptic abnormalities of autosome and sex bivalents during the pachytene. It was indicated that EM analysis of SC-spermatocytes—the study of synaptonemal complexes in agricultural animals—is a very good instrument for comparative analysis of normal SC-karyotype of foxes that carry chromosomal abnormalities and in fur-bearing animals as well. 相似文献
3.
The results of light and electron microscopic (EM) studies of meiosis in Microtus arvalis males of the karyoform "arvalis" (2n = 46, NFa = 80), in hybrids between the chromosomal forms arvalis and obscurus (2n = 46, NFa = 68), in M. rossiaemeridionalis voles (2n = 54, NFa = 54), and in a hybrid between the species M. rossiaemeridionalis and M. kermanensis (2n = 54, NFa = 54) are presented. SC (synaptonemal complex) karyotypes of the parental forms and the hybrids were constructed on the basis of measurements of the length ofautosomal SCs revealed by the EM analysis in spermatocytes at the stage of middle pachytene. The SC karyotypes of M. arvalis and the hybrids female obscurus x male arvalis consist of 22 synaptonemal complexes of autosomal bivalents and the axial elements of the synaptonemal complexes of the sex chromosomes X and Y. The SC karyotypes of M. rossiaemeridionalis and the hybrid M. rossiaemeridionalis x M. kermanensis consist of 26 synaptonemal complexes of autosomal bivalents and a sex bivalent; they differ only in the length of the Y chromosome axis (Y chromosome in the hybrid was inherited from M. kermanensis). Asynaptic configurations of the autosomal SCs were not observed in the hybrids. The SC axial elements of the X and Y chromosomes in the parental forms and in the hybrids were located close to each other throughout pachytene, but they did not form a synaptic region. The normal synapsis in sterile hybrids (M. rossiaemeridionalis x M. kermanensis) and the behavior of the sex chromosomes in meiosis in fertile and sterile hybrids are discussed in the context of specific features of meiosis and reproductive isolation. 相似文献
4.
The karyotype with C-, G- and NOR-banding of Arctocephalus australis is reported for the first time. The chromosomal number is 2n = 36. The X chromosome, identified in G-banded metaphases from males, is metacentric and the Y chromosome is a minute chromosome, also metacentric. Pachytene spermatocytes were used for synaptonemal complexes analysis with a surface spreading technique. A total of 17 autosomal synaptonemal complexes are observed plus the XY pair. During early pachytene, the X and Y axes are thickened and remain unpaired. As pachytene advances, a short SC is formed between the gonosomes, as it is common among eutherian mammals. The particular asymmetrical appearance of the synaptonemal complex in the sex pair is described and compared to other cases among mammals. 相似文献
5.
Stage-specific damage to synaptonemal complexes and metaphase chromosomes induced by X rays in male mouse germ cells 总被引:1,自引:0,他引:1
Synaptonemal complexes reveal mutagen-induced effects in germ cell meiotic chromosomes. This study was aimed at characterizing relationships between damage to synaptonemal complexes and metaphase I chromosomes following radiation exposure at various stages of spermatogenesis. Male mice were irradiated with doses of 0, 2, or 4 Gy, and spermatocytes were harvested at times consistent with earlier exposures as spermatogonial stem cells, preleptotene cells (premeiotic DNA synthesis), or meiotic prophase cells. After stem-cell exposure, twice as many rearrangements were observed in synaptonemal complexes as in metaphase I chromosomes. Irradiation during premeiotic DNA synthesis resulted in dose-related increases in synaptonemal complex breakage and rearrangements (including novel forms) and in metaphase chromosomal aberrations. Following prophase exposure, various types and levels of damage to synaptonemal complexes and metaphase chromosomes were observed. Irradiation of zygotene cells led to high frequencies of chromosome multivalents in metaphase I without a correspondingly high level of damage in preceding prophase synaptonemal complexes. Thus irradiation of premeiotic and meiotic cells results in variable relationships between damage to synaptonemal complexes and metaphase chromosomes. Interpretations of these relationships are based upon what is known about both radiation clastogenesis and the structural/temporal relationships between synaptonemal complexes at prophase and chromosomes at metaphase I of meiosis. 相似文献
6.
L. D. Safronova F. N. Golenishchev E. V. Cherepanova M. I. Baskevich 《Russian Journal of Genetics》2011,47(7):857-863
The results of light and electron microscopic (EM) studies of meiosis in Microtus arvalis males of the karyoform “arvalis” (2n = 46, NFa = 80), in hybrids between the chromosomal forms arvalis and obscurus (2n = 46, NFa = 68), in M. rossiaemeridionalis voles (2n = 54, NFa = 54), and in a hybrid between the species M. rossiaemeridionalis and kermanensis (2n = 54, NFa = 54) are presented. SC (synaptonemal complex) karyotypes of the parental forms and the hybrids were constructed
on the basis of measurements of the length of autosomal SCs revealed by the EM analysis in spermatocytes at the stage of middle
pachytene. The SC karyotypes of M. arvalis and the hybrids ♀ obscurus × ♂ arvalis consist of 22 synaptonemal complexes of autosomal bivalents and the axial elements
of the synaptonemal complexes of the sex chromosomes X and Y. The SC karyotypes of M. rossiaemeridionalis and the hybrid M. rossiaemeridionalis × M. kermanensis consist of 26 synaptonemal complexes of autosomal bivalents and a sex bivalent; they differ only in the length of the Y chromosome
axis (Y chromosome in the hybrid was inherited from M. kermanensis). Asynaptic configurations of the autosomal SCs were not observed in the hybrids. The SC axial elements of the X and Y chromosomes
in the parental forms and in the hybrids were located close to each other throughout pachytene, but they did not form a synaptic
region. The normal synapsis in sterile hybrids (M. rossiaemeridionalis × M. kermanensis) and the behavior of the sex chromosomes in meiosis in fertile and sterile hybrids are discussed in the context of specific
features of meiosis and reproductive isolation. 相似文献
7.
The synaptonemal complex (SC) was analyzed in four F1 hybrids of Bos taurus taurus and B. taurus indicus including Gyr-Simmental (G-S), Nelore Simmental (N-S), Gyr-Holstein-Friesian (G-H) and Nelore-Piemontese (N-P). We analysed the frequency of various types of SC abnormalities and the frequency of cells with SC abnormalities. The results were compared with similar observations made on purebred animals. All the animals studied possessed 29 autosomal and one sex bivalent. The frequency of cells with abnormalities in the hybrids were 28.0% in the N-P, 29.1% in the G-S, 33.3% in the N-S and 40.0% in the G-H. The frequency of cells with abnormalities in the four hybrids was 31.5%; 57.9% of these abnormalities occurred in zygotene and 42.0% occurred in pachytene. The comparisons among the hybrids and among the hybrids and their parental breeds showed that the only significant difference was between Gyr and Gyr-Holstein-Friesian animals. Some aspects of the relationship between the frequency of cells with anomalies and the fertility of hybrids are discussed. 相似文献
8.
The ability of two inactivated bacterial vaccines, from Proteus vulgaris and Klebsiella pneumoniae, to injure synaptonemal complexes (SCs) was studied by means of electron microscopy. The preparations were given intraperitoneally to C57BL/6J male mice during five successive days and testes were fixed 24 h after the last injection. Cyclophosphamide was used for positive control. The vaccine from Klebsiella given at subtoxic dose, which was about 200 times higher that that given to people during vaccinations, induced the 10-fold rise in the frequency of SC abnormalities in murine 1-storger spermatocytes. Breaks of SCs and of single lateral elements of SCs predominated over other types of vaccine-induced anomalies. According to the preliminary data, vaccine from Proteus at subtoxic dose showed no damaging SC activity. The results of the given study are discussed in connection with the negative data obtained earlier when genotoxicity of these two vaccines had been studied in the Ames test, in routine investigations of bone marrow metaphases of vaccinated mice as well as under light microscopy of their SCs. 相似文献
9.
Electron microscopic analysis of synaptonemal complexes and analysis of chiasmata distribution in male mice heterozygous for Robertsonian translocation T(16; 17)7Bnr - (Rb7), for synaptonemal reciprocal translocation T(16;17)43H - (T43), in double heterozygotes for these translocations and in males with partial trisomy of the proximal region of chromosome 17 was carried out. Synaptic disturbances around the breakpoints of the translocations, such as asynapsis of homologous regions of partners and non-homologous synapsis of centromeric regions of acrocentric chromosomes, were revealed. Synaptic regularity in the proximal part of the chromosome 17 appeared to be affected by no t12 haplotype. Good coincidence between sizes of mitotic chromosomes and corresponding lateral elements of synaptonemal complexes was found for all chromosomes, with the exception of Rb7 in trisomics. In the latter karyotype, the proximal part of chromosome 17 involved in Robertsonian fusion seems to be shortened in the course of zygotene and never synapted with homologous segment of neither the acrocentric chromosome 17 nor large product of reciprocal translocation. Drastic increase in chiasmata frequency in the proximal part of chromosome 17 was revealed in heterozygotes for T43H and in trisomics, as compared with the double heterozygotes Rb7/T43. The latter finding was explained by the existence of two independent pairing segments in the former karyotypes. 相似文献
10.
Synaptonemal complex abnormalities are frequent in reconstructed meiotic prophase nuclei of Neurospora crassa and Neurospora intermedia. Three kinds of synaptonemal complex anomalies were seen: lateral component splits, lateral component junctions, and multiple complexes. The anomalies apparently are formed during or after the pairing process, as they were not seen in the largely unpaired early zygotene chromosomes. Their presence at all the other substages from mid-zygotene to late pachytene indicates that they are not eliminated before the synaptonemal complex decomposes at diplotene. Abnormal synaptonemal complexes were seen in all 19 crosses of N. crassa and N. intermedia that were examined, including matings between standard laboratory strains, inversions, Spore killers, and strains collected from nature. The frequency of affected nuclei and degree of abnormality within a nucleus varied in different matings. No abnormalities were present in the homothallic species Neurospora africana and Neurospora terricola. Structural chromosome aberrations, introgression, and heterozygosity have been eliminated as causes for pairing disorder. The abnormal synaptonemal complexes seemingly do not interfere with normal ascus development and ascospore formation. The affected nuclei are not aborted during meiotic prophase, nor are they eliminated by abortion of mature asci. The abnormal meiocytes do not lead to aneuploidy, as judged by the low frequency of white ascospores in crosses between wild type strains that have many abnormalities. Thus, the abnormal synatonemal complexes do not appear to prevent chiasma formation between homologues. 相似文献
11.
Spermatogenesis is inhibited in Balb/C mice as a result of oxyradical insult. However, mammalian spermatocytes and synaptonemal complexes retain their structure and function after oxyradical insult due to protection afforded by the antioxidant vitamin E. Control groups were compared with experimental groups which were fed various vitamin E-deficient diets and subjected to varying times in an humidified 100% oxygen (hyperoxia) chamber. Measurements were made of sex body volume (SBV), nuclear envelope aberrations (NEA), and synaptonemal complex structure in spermatocytes during pachytene of meiosis prophase I. Changes in the volume of the sex body were positively correlated with increased oxyradical insult. The structure of the synaptonemal complex was not altered in any of the experimental groups which is a significant observation. It is suggested that vitamin E affords antioxidant protection and inhibits the alteration of membranes and sex chromosomes in mice during meiosis. 相似文献
12.
J Vilardell M D Coll E Querol J Egozcue 《Cellular and molecular biology, including cyto-enzymology》1989,35(2):207-214
The presence of a stoechiometric electrophoresis pattern of histones in carefully isolated synaptonemal complexes is reported. The use of this pattern is suggested as an internal standard of synaptonemal complex purification, in addition to the more generally used electron micrographs. This is especially useful in experiments leading to the characterization of the protein components of SCs. The use of mice of an age at which pachytenes predominate (90%) in the prophase-cell population is also advantageous to improve the final yield in synaptonemal complexes. 相似文献
13.
C. B. Gillies 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1987,74(4):430-438
Summary Chromosome pairing at zygotene-pachytene was studied in Triticum aestivum × T. kotschyi hybrids (2n=5x=35, genomic constitution ABDCUSv) by electron microscopy of synaptonemal complexes in spread microsporocyte nuclei. Hybrids carrying either the Ph allele or the ph allele, which differ markedly in metaphase I pairing, are both capable of greater than 90% pachytene pairing, although pairing in the Ph hybrids appeared slower or less synchronised. In both genotypes branched synaptonemal complexes were formed by intra-and interchromosomal pairing. The Ph gene control on homoeologous pairing does not act on the ability to pair into synaptonemal complexes. It may act on the rate of pairing or the time of crossing over. 相似文献
14.
Synaptonemal complexes in Gerbillidae: probable role of intercalated heterochromatin in gonosome-autosome translocations 总被引:1,自引:0,他引:1
C Ratomponirina E Viegas-Péquignot B Dutrillaux F Petter Y Rumpler 《Cytogenetics and cell genetics》1986,43(3-4):161-167
A study of sex chromosomes and synaptonemal complexes in male specimens of Gerbillus chiesmani, G. nigeriae, G. hoogstrali, and Taterillus pygargus is reported. In each of these Gerbillidae species there are two or three translocations of autosomes with X and Y chromosomes. Analysis of mitotic chromosomes consistently shows the presence of constitutive heterochromatin on the der t(X;autosome) at the X-autosome junction and on the der t(Y;autosome). Analysis of the synaptonemal complexes shows the existence of an unusual structure, lightly stained, at the X-autosome junction and at the Y-autosome junction, which is probably heterochromatic in nature, thus corresponding to the mitotic patterns. This heterochromatin separates the autosomal and gonosomal segments, which behave independently and normally. By analogy with findings from humans and other mammals, a general hypothesis is proposed on the role of intercalated heterochromatin between translocated gonosomes and autosomes. This hypothesis explains why the pathological consequences of these translocations may be very different in males and females. The role of intercalated heterochromatin would be to avoid the pathological consequences of gonosome-autosome translocations resulting from inactivation of the sex chromosomes in female somatic cells and male germinal cells. 相似文献
15.
The pattern of chromosomal pairing was analyzed in male deer mice (Peromyscus maniculatus and Peromyscus sitkensis) heterozygous for the presence of heterochromatic short arms. G- and C-banding of somatic metaphases indicated that the presence of heterochromatic short arms increased the length of chromosome 4 by 15% in P. sitkensis and that of chromosome 8 by 9% in P. maniculatus. Analysis of silver-stained late zygotene and early pachytene nuclei revealed a low frequency of unequal axial lengths in the synaptonemal complexes corresponding to the heteromorphic bivalents. All mid- and late pachytene nuclei, however, exhibited fully paired synaptonemal complexes with equalized axial lengths. These observations suggest the existence of an adjustment mechanism which functions to equalize the lengths of the two axes of the heteromorphic synaptonemal complex. 相似文献
16.
Identification of two major components of the lateral elements of synaptonemal complexes of the rat 总被引:12,自引:0,他引:12
C Heyting P B Moens W van Raamsdonk A J Dietrich A C Vink E J Redeker 《European journal of cell biology》1987,43(1):148-154
This paper describes the identification of two major components of the lateral elements of synaptonemal complexes of the rat by immunocytochemical techniques. We prepared monoclonal antibodies against synaptonemal complexes (SCs) by immunization of mice with purified SCs. One of these antibodies, II52F10, reacts with a 30 and a 33 kDa polypeptide, which are major components of purified SCs. Using this antibody, we studied the localization of its antigens light microscopically, by means of the indirect immunoperoxidase technique, as well as ultrastructurally, by means of the immunogold labeling technique. The immunolocalization was carried out on whole-mount preparations of lysed spermatocytes. The antibody reacts with paired as well as unpaired segments of zygotene, pachytene and diplotene SCs. In light microscopic preparations, the attachment plaques, particularly those of late pachytene and diplotene SCs, also appear to react strongly. In electron micrographs the lateral elements in paired as well as unpaired segments could be seen to react. No reaction was observed in the attachment plaques; however, in late pachytene and diplotene SCs the swollen terminal segments of the lateral elements did react with the antibody. Thus, we conclude that a 30 and a 33 kDa polypeptide make part of the lateral elements of synaptonemal complexes of the rat. 相似文献
17.
中国人精母细胞和卵母细胞联会复合体的电镜观察 总被引:4,自引:0,他引:4
以微铺展技术结合硝酸银染色,对中国人精母细胞和流产胎儿卵巢联会复合体的形态和行为作了电镜观察。列出中国人的SC核型和模式图。根据减数分裂前期XY的复杂形态变化,XY的配对可分为5种类型。对XY短臂之间形成的SC和XY长臂顶端的次级联合以及XY配对的性质和机理作了描述和讨论。本文还报道了一个罕见的三倍体精母细胞,对三倍体精母细胞中SC的配对行为以及和人类染色体疾病病因的可能关系作了分析和讨论。 相似文献
18.
Analysis of the chromosome behaviour at pachytene has been performed by means of the silver staining technique visualizing the synaptonemal complexes (SCs) in male mice heterozygous for the male-sterile translocations T(5;12)31H, T(16;17)43H and T(7;19)145H, respectively. The T(9;17)138Ca male heterozygotes and T43H/T43H homozygous males were used as fertile controls. The sterile mice displayed a high frequency (about 60%) of pachytene spermatocytes with autosomal translocation configuration located in close vicinity of the XY pair. The dense round body (XAB), normally located near the X-chromosome axis in fertile males, exhibited abnormal affinity to translocation configuration in the sterile translocation heterozygotes. The incomplete synapsis of autosomes involved in translocation configuration was observed in more than 70% of the pachytene spermatocytes with the male-sterile translocations but in less than 20% of the cells from T138Ca fertile male.s. A hypothesis relating the spermatogenic arrest of carriers of male-sterile rearrangements to the presumed interference with X chromosome inactivation in male meiosis is discussed. 相似文献
19.
Synaptonemal complexes of the repopulating spermatocytes of male rats were analyzed day by day using silver-stained surface spread nuclei between 8 and 25 days after restricting the germ cell population to spermatogonia by treatment of gossypol acetic acid at 30 mg/kg body weight/day for 70 days. The method allowed sequential analysis of male meiotic prophase on successive days after the last day of treatment. The leptotene cells appeared on day 11 and were characterized by a network of lateral elements and large nucleolar bodies in a diffuse mass. On day 13 the unpaired lateral elements and short stretches of synaptonemal complexes characteristic for zygotene could be seen. Pachytene nuclei showing 20 autosomal synaptonemal complexes and XY axes appeared on day 15. The diplotene cells were defined on day 22 by the loss of a complete synaptonemal complex set and by the appearance of disjoined lateral elements and persistent segments of synaptonemal complexes. 相似文献
20.
We describe the behaviour of synaptonemal complexes (SCs) in Syrian hamster and mouse oocytes. InMesocricetus auratus, synaptonemal complexes can be observed from birth up to 7 days of life. In foetuses fromMus musculus, synaptonemal complexes can be observed from the 14th day of gestation up to the first day post-partum, when the cells enter
the dictyotene stage. In both species, synaptonemal complexes show, in general, the same morphology described in male cells
by light and electron microscopy, with the exception that the axes of the sex bivalent are not identifiable. The leptotene
stage can be identified although it is probably of short duration. Only one type of zygotene (zygote ne II of Dietrich and
Mulder(Chromosoma 88: 377), 1983) has been observed. In the hamster we also describe a desynaptic diplotene stage previous
to the desintegration of the SCs. In oocytes from both species late pairing (or precocious separation) of a single bivalent
can be seen in a few cells. Interlocking of some bivalents with delayed pairing of the affected region is rather frequent.
Furthermore, hamster oocytes may show heterosynapsis of the telomeres of autosomal bivalents by pachytene. 相似文献