红圆蚧3种寄生蜂的寄生作用研究

研究了红圆蚧（Ａｏｎｉｄｉｅｌｌａａｕｒａｎｔｉｉ）３种寄生蜂（盾蚧长缨蚜小蜂Ｅｎｃａｒｓｉａｃｉｔｒｉ－ｎａ,岭南黄蚜小蜂Ａｐｈｙｔｉｓｌｉｎｇｎａｎｅｓｉｓ,印巴黄蚜小峰Ａｐｈｙｔｉｓｍｅｌｉｎｕｓ）在不同温度、不同寄生物密度、不同寄主密度组合下的功能反应．攻击率印巴蜂最大,岭南蜂次之,长缨蚜小蜂蜂最小．３种寄生蜂在不同温度下的攻击率都近于正态分布．建立了含干扰效应、温度效应的功能反应模型,两个模型的各参数均有明确的生物学意义,模拟效果好．     

红圆蚧三种寄生蜂的相互竞争研究

本文研究了红圆蚧（Ａｏｎｉｄｉｅｌｌａａｕｒａｎｔｉｉ）三种寄生蜂：盾蚧长缨蚜小峰（Ｅｎｃａｒｓｉａｃｉｔｒｉｎａ,ＥＣ）、岭南蚜小蜂（Ａｐｈｙｔｉｓｌｉｎｇｎａｎｅｓｉｓ,ＡＬ）和印巴黄蚜小蜂（Ａｐｈｙｔｉｓｍｅｌｉｎｕｉｓ,ＡＭ）的相互竞争。结果表明,ＥＣ蜂寄生过的寄主对ＡＭ蜂和ＡＬ蜂没影响,ＡＭ蜂能辩认被ＡＬ蜂寄生过的寄主,反之亦然,幼虫竞争能力ＡＬ蜂最强,其次ＡＭ蜂,最差为ＥＣ蜂。不同的寄主龄期产生不同的竞争结果：当ＡＭ蜂和ＡＬ蜂竞争时,若只供给３龄寄主则ＡＬ蜂获胜,若供给只有２龄寄主则ＡＭ蜂获胜;而ＡＭ或ＡＬ蜂与ＥＣ竞争时。若大多数寄主为２龄则ＥＣ蜂获胜,若大多数寄主为３龄则ＡＭ或ＡＬ蜂获胜。     

安徽虫瘟霉菌株的强毒杀蚜应与侵染速率

报道安徽虫瘟霉（Ｚｏｏｐｈｔｈｏｒａ ａｎｈｕｉｅｎｓｉｓ）菌株Ｆ９７０２８对桃蚜（Ｍｙｚｕｓ ｐｅｒｓｉｃａｅ）的强毒杀蚜活性。以７个孢子剂量（０．４￣１０．４个孢子／ｍｍ＾２）接种２￣３龄若蚜（５２￣８６头／剂量）,连续观察７ｄ,所获数据经时间－剂量－死亡率模型模拟分析,接种后第３￣７ｄ的ＬＤ５０分别为３４．８、８７、１．５、０．７和０．４个孢子／ｍｍ＾２;在所有剂量范围内ＬＴ５０为２．９￣６     

银杏叶片磷脂酰甘油脂肪酸分子种组成及其位置分布

用高效液相色谱法和酶解的方法检测了银杏叶片磷脂酰甘油（ＰＧ）脂肪酸的分子种组成和位置分布,确定银杏叶片ＰＧ主要分子种的脂肪酸组成（ｓｎ１／ｓｎ２）是１８∶３／１６∶１（３ｔ）,１８∶３／１６∶０,１８∶２／１６∶１（３ｔ）,１８∶２／１６∶０,１８∶１／１６∶１（３ｔ）,１６∶０／１６∶１（３ｔ）,１８∶１／１８∶１,１８∶１／１６∶０和１６∶０／１６∶０。银杏叶片ＰＧ脂肪酸组成和位置分布的分析结果表明,Ｃ１８脂肪酸主要位于ｓｎ１位,１６∶１（３ｔ）只分布于ｓｎ２位,１６∶０在ｓｎ１位和ｓｎ２位上均有发现。ｓｎ１位上的不饱和度∑ｕ大于ｓｎ２位上的∑ｕ。     

刺槐宽叶和四倍体无性系的组织培养

１植物名称刺槐（Ｒｏｂｉｎｉａｐｓｅｕｄｏａｃａｃｉａ）优良无性系：Ｔｅｔｒａｐｌｏｉｄｌｏｃｕｓｔ、Ｇｌｇａｓｔｙｐｅｌｏｃｕｓｔ。２材料类别带腋芽的茎段。３培养条件（１）启动培养基：ＭＳ＋６－ＢＡ０．２５ｍｇ·Ｌ－１（单位下同）＋ＮＡＡ０．０５。（２）分化培养基和继代培养基：ＭＳ＋６－ＢＡ０．５＋ＮＡＡ０．１＋ＡｇＮＯ３１０,ＭＳ＋６ＢＡ０．５＋ＮＡＡ０．１。上述培养基均添加３％蔗糖、０．６％琼脂。（３）生根培养基：１／２ＭＳ＋ＩＢＡ０．２＋ＮＡＡ０．２,添加２％蔗糖０．６％琼脂。培养基ｐＨ…     

竹类害虫的六种寄生蜂及二新种记述（膜翅目：跳小峰科）

本文报导与竹类害虫有关的６种跳小蜂。长缘刷盾跳小蜂Ｃｈｅｉｌｏｎｅｕｒｕｓｃｌａｖｉｇｅｒ及优赛跳小蜂Ｅｕｓｅｍｉｏｎｃｏｒｎｉｇｅｒｕｍ,寄生竹巢粉蚧Ｎｅｓｔｉｃｏｃｃｕｓｓｉｎｅｎｓｉｓ的初寄生者花翅跳小蜂Ｍｉｃｒｏｔｅｒｙｓｓｐｐ．;瓢虫隐尾跳小蜂Ｈｏｍａｌｏｔｙｌｕｓｆｌａｍｉｎｉｕｓ,寄生黑缘光瓢虫Ｅｘｏｃｈｏｍｕｓ（Ｘａｎｔｈｏｃｏｒｎｓ）ｎｉｇｒｏｍａｒｇｉｎａｔｕｓ;白胫短缘跳小蜂Ｚａｏｍｍａｅｒｉｏｃｏｃｉ（Ｔａｃｈｉｋａｗａ）中国新记录,寄生竹巢粉蚧的初寄生者花翅跳小蜂;四斑长索跳小蜂ＡｎａｇｙｒｕｓｑｕａｄｒｉｍａｃｕｌａｔｕｓＸｕｅｔＨｅ,ｓｐ．ｎｏｖ,寄生竹绒粉蚧Ｅｒｉｏｃｏｃｃｕｓｔｒａｎｓｖｅｒｓｕｓ;长脉卵跳小蜂ＯｏｅｎｃｙｒｔｕｓｌｏｎｇｉｖｅｎｏｓｕｓＸｕｅｔＨｅ,ｓｐ．ｎｏｖ,寄生竹卵圆蝽Ｈｉｐｐｏｔｉｓｃｕｓｄｏｒｓａｌｉｓ。标本及模式标本保存在浙江农业大学寄生蜂标本室。     

镰孢属几种植物病原真菌分子核型的研究

利用脉冲电场凝胶电泳（ｐｕｌｓｅｄ－ｆｉｅｌｄｇｅｌｅｌｅｃｔｒｏｐｈｏｒｅｓｉｓ,ＰＦＧＥ）,研究了４株串珠镰孢（Ｆｕｓａｒｉｕｍ　ｍｏｎｉｌｉｆｏｒｍｅ）、１株尖镰孢（F．ｏｘｙｓｐｏｒｕｍ）、１株茄镰孢（Ｆ．ｓｏｌａｎｉ）和１株Ｆｕｓａｒｉｕｍｓｐ．的分子核型以及不同地域和寄主来源的串珠镰孢种内菌株间的分子核型差异。以凝胶包埋法（不破除分生孢子细胞壁）制备供试菌株电泳样本,采用３组条件组合进行电泳,分离出供试串珠镰孢完整染色体ＤＮＡ１０～１３条,分子量分布范围０．７Ｍｂ～６．９Ｍｂ,基因组大小为４２．２６Ｍｂ～４７．７５Ｍｂ;尖镰孢８条,分子量分布范围１．２Ｍｂ～６．７Ｍｂ,基因组大小为３２．２５Ｍｂ;茄镰孢６条,分子量分布范围２．４Ｍｂ～６．３Ｍｂ,基因组大小为２５．２Ｍｂ;Ｆｕｓａｒｉｕｍｓｐ．９条,分子量分布范围０．８Ｍｂ～６．８Ｍｂ,基因组大小为３６．４５Ｍｂ。结果表明,供试４种镰孢菌染色体数目、ＤＮＡ分子量及基因组大小都有较大不同,分子核型差异较大。不同来源的串珠镰孢种内菌株间分子核型亦有明显差异。     

福建海产鱼类寄生吸虫二新种（ 吸虫纲：复殖目：隐殖科）

从福建沿海的鲈鱼肠道里查获双巢亚科的厦门双巢吸虫,新种Ｂｉｏｖａｒｉｕｍｘｉａｍｅｎｅｎｓｅｓｐ．ｎｏｖ,虫体大小２．５４～４．２０ｍｍ×０．３０～０．４０ｍｍ（２．９０３×０．３６９ｍｍ）,虫卵１８～２２μｍ×１０～１４μｍ（２０．４×１１μｍ）;从勒氏菌鲷的小肠里检获新隙亚科的厦门拟隐殖吸虫,新种Ｐａｒａｃｒｙｐｔｏｇｏｎｉｍｕｓｘｉａｍｅｎｅｎｓｉｓｓｐｎｏｖ,虫体大小０．８００～０．１６７２ｍｍ×０．４８４～０．８４８ｍｍ（１．２４７×０．６８５ｍｍ）,虫卵１９～３０μｍ×１１～１４μｍ（２４×１２μｍ）,头棘５５枚。     

镰孢属几种植物病原真菌分子核型的研究*

利用脉冲电场凝胶电泳（ｐｕｌｓｅｄ－ｆｉｅｌｄｇｅｌｅｌｅｃｔｒｏｐｈｏｒｅｓｉｓ,ＰＦＧＥ）,研究了４株串珠镰孢（Ｆｕｓａｒｉｕｍ　ｍｏｎｉｌｉｆｏｒｍｅ）、１株尖镰孢（F．ｏｘｙｓｐｏｒｕｍ）、１株茄镰孢（Ｆ．ｓｏｌａｎｉ）和１株Ｆｕｓａｒｉｕｍｓｐ．的分子核型以及不同地域和寄主来源的串珠镰孢种内菌株间的分子核型差异。以凝胶包埋法（不破除分生孢子细胞壁）制备供试菌株电泳样本,采用３组条件组合进行电泳,分离出供试串珠镰孢完整染色体ＤＮＡ１０～１３条,分子量分布范围０．７Ｍｂ～６．９Ｍｂ,基因组大小为４２．２６Ｍｂ～４７．７５Ｍｂ;尖镰孢８条,分子量分布范围１．２Ｍｂ～６．７Ｍｂ,基因组大小为３２．２５Ｍｂ;茄镰孢６条,分子量分布范围２．４Ｍｂ～６．３Ｍｂ,基因组大小为２５．２Ｍｂ;Ｆｕｓａｒｉｕｍｓｐ．９条,分子量分布范围０．８Ｍｂ～６．８Ｍｂ,基因组大小为３６．４５Ｍｂ。结果表明,供试４种镰孢菌染色体数目、ＤＮＡ分子量及基因组大小都有较大不同,分子核型差异较大。不同来源的串珠镰孢种内菌株间分子核型亦有明显差异。     

松突圆蚧黑蚜小蜂的产卵分布

采用m*－m方法分析了黑蚜小蜂的产卵频数分布,在25种密度组合下,产卵平均拥挤度m*的值从0．09（1：80）增加到2．96（9：5）,并建立了m*与蜂蚧比、m*与平均密度m的回归模型。结果表明,松突圆蚧黑蚜小蜂产复寄生卵的程度与寄生蜂密度呈正相关,与寄主密度呈负相关,产卵呈随机分布。     

Host selection and egg allocation behaviour by Aphytis melinus and A.lingnanensis: comparison of two facultatively gregarious parasitoids

Abstract. 1. The ovipositional and egg allocation behaviour of individual females of Aphytis melinus DeBach and A.lingnunensis Compere were compared.
2. Both Aphytis species exhibit the same behavioural sequence during oviposition.
3. Aphytis melinus laid most of its female eggs on the dorsum of a scale-insect beneath its cover, and most of its male eggs under the scale-insect's body. Aphytis lingnanensis also oviposited both dorsally and ventrally on scale-insect hosts, but female and male progeny arose with equal frequency from eggs laid in both locations.
4. Both A.melinus and A. lingnanensis are facultatively gregarious parasitoids. The degree of gregariousness depends on host size, i.e. the larger the host, the more the Iikelihood that several eggs will be deposited at each visit by the parasitoid.
5. When two eggs were laid during the same host visit, both A.melinus and A.lingnanensis laid one female and one male egg more often than would be expected under an assumption of random allocation of sexes.
6. Because A.melinus successfulIy utilize smaller hosts than A.lingnanensis to produce progeny, these parasitoids should not be considered ecological homo-logues, as suggested by DeBach & Sundby (1963).     

一种松突圆蚧寄生蜂Aphytis sp.的寿命与产卵研究

在19～32℃内,不供给蜂蜜时,Aphytis sp.寿命是0.8～3.1天,供给蜂蜜时,寿命可延长到3.6～15.2天.Aphytis sp.在25℃和28℃产卵量最多,分别为64.2/♀和63.0/♀,19℃和32℃分别为26.7/♀和27.8/♀.每天每雌产卵2.67～5.35个,产卵集中前5天,占总产卵量的57.6～87.0%.一般第2～3天为产卵高峰日。使用两种方法计算了 Aphytis sp.的内禀增长率,建立了世代产卵量与温度模型和逐日产卵量模型.     

REVISION OF THE GENUS APHYTIS (HYMENOPTERA: APHELINIDAE) WITH DESCRIPTIONS OF TWO NEW SPECIES FROM EGYPT

Abstract  The Egyptian species of the aphelinid genus Aphytis Howard are reviewed. Aphytis azai Abd-Rabou and Aphytis matruhi Abd-Rabou are described as new species from Egypt. Each species is briefly diagnosed and known information on hosts and distributed is given. A. azai sp. nov. is similar to A. melinus but different in the measurement of antennal segments. A. matruhi sp. nov. differs from A. lepidosaphes by the relative length of propodeum, metanotum and scutellum. A key to the Egyptian species of Aphytis is provided.     

埃及Aphytis黄蚜小蜂属记述附二新种描述（膜翅目：蚜小蜂科）

对埃及的黄蚜小蜂属进行了综述,并描述了2个新种,即Aphytis azai Abd—Rabou和Aphytis matruhi Abd—Rabou。文章对产自埃及的17个种做了简要描述,还提供了寄主和分布信息,以及埃及黄蚜小蜂属的分种检索表。新种A．azai sp．nov．与A．melinus相似,但区别在于触角节的大小。新种A．matruhi sp．nov．区别于A．lepidosaphes之处在于间节、后胸腹板以及小盾片的相对长度不同。     

中国黄蚜小蜂属二新种(膜翅目:蚜小蜂科)

本文记述了在园林害虫天敌普查中从蚧虫内饲养出的我国黄蚜小蜂属Aphytis Howard两新种。模式标本存广东省昆虫研究所。 1、樟雪蚧黄蚜小蜂Aphytis chionaspis Ren,新种(图1—8) 雌:长0.75—1.05毫米。体黄色。头部复眼紫红色。触角柄节淡黄色,鞭节微暗色。头、胸、腹部刚毛淡褐色。小盾片后端缘中部黑褐色。胸部腹板暗色、叉状纵干黑褐色。翅透明,前翅翅基片下方一短的横条骨片褐色。缘脉与亚缘脉连接处之下方暗色,后缘褶淡褐色。前足跗节暗色;中、后足胫节、跗节暗色;后足腿节腹缘淡色。产卵管淡褐色。     

韩国黄蚜小蜂属分类研究(膜翅目:蚜小蜂科)

本文报道采自韩国的黄蚜小蜂属5种,包括1新种（Aphytis albus,sp．nov．)及4个韩国新记录种(Adiaspidis,A.japonicus,Aproclia和Avandenboschi),编制了雌成虫检索表。新种模式标本保存在韩国国立树木园和东北林业大学昆虫标本室。     

Adult feeding and lifetime reproductive success in the parasitoid Aphytis melinus

The diet of adult females of the parasitoid Aphytis melinus DeBach (Hymenoptera: Aphelinidae) includes host insects and sugar-rich foods such as nectar and honeydew. We compared the contributions of host feeding to longevity and fecundity in A. melinus females in the presence and in the absence of honey meals. First, we assessed the longevity of females that were not allowed to oviposit. While the longevity of females fed honey was significantly increased by host feeding (median ages were 30.5 days for host-fed females and 17 days for females not allowed to host feed), the lifespan of parasitoids not fed honey did not exceed 3 days for any individual and there was no effect of host feeding on longevity in this group. In the second set of experiments, we assessed the fecundity and longevity of females allowed to oviposit. We conducted two experiments, one in which honey was continuously available, and one in which honey was not available. In both experiments, daily observations were made of females that were either allowed to host feed or manually prevented from host feeding. In the presence of honey, host feeding significantly increased both fecundity and longevity, and in the absence of honey, parasitoids died within 2 days and host feeding had no significant effect on either fecundity or longevity. The lifetime fecundity of females fed honey but not hosts exceeded the initial egg complement by 60% on average. Approximately one host per day was used for host feeding whether honey was supplied or not, and each host-feeding meal contributed approximately 3.9 eggs to the lifetime fecundity of honey-fed females. In the last experiment, we compared the rate of egg resorption over a 36-h period in A. melinus females that were deprived of hosts and either fed honey or starved. While no egg resorption was detected in honey-fed females over this time period, starved females resorbed approximately 9 eggs. Thus, the availability of a sugar-rich food interacts strongly with host feeding in influencing longevity and fecundity and has a strong direct effect on egg resorption.     

Primary parasitoids of red scale (Aonidiella aurantii) in Australia and a review of their introductions from Asia

We used morphological and molecular differences to confirm the identities of red scale (Aonidiella aurantii) and yellow scale (A. citrina), and their primary parasitoids, in Australia. An extension to the distribution of yellow scale was confirmed. Six primary parasitoids of red scale were identified: Aphytis chrysomphali, A. lingnanensis, A. melinus, Comperiella bifasciata, Encarsia citrina, and E. perniciosi. With the exception of A. lingnanensis, these parasitoids, and a species of Aphelinus, were detected in association with red scale during studies in citrus orchards in coastal New South Wales between 2009 and 2012. Two races of A. melinus were recorded: one from the Indian Subcontinent, the other previously only recorded in China. The studies, and reviews of historical records, led us to conclude that 4 parasitoids, A. lingnanensis, C. bifasciata, and both species of Encarsia, were present in Australia before successful or unsuccessful formal introductions between 1902 and 1970. The A. melinus race previously recorded in China may also have been present before the Indian Subcontinent race was formally introduced in 1961. We suggest the possibility that the natural distribution of some of the parasitoids may include East and Southeast Asia, and parts of Australasia. We found no reports of native armored scales being recorded on species and hybrids of Citrus introduced to Australia, and no reports of introduced armored scales being recorded on native Rutaceae, including 6 species of Citrus. However, we subsequently recorded yellow scale on Geijera parviflora, a native rutaceous tree.     

中国黄蚜小蜂属一新记录种及其蛹的个体发育

记述了中国黄蚜小蜂属一新记录种—桑氏黄蚜小蜂 Aphytis sankarani Rosen et DeBach，描述了桑氏黄蚜小蜂的形态特征，观察了蛹的个体发育，并提供了重要鉴别特征的照片。