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Contour-Ansel D Torres-Franklin ML Cruz DE Carvalho MH D'Arcy-Lameta A Zuily-Fodil Y 《Annals of botany》2006,98(6):1279-1287
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Torres-Franklin ML Contour-Ansel D Zuily-Fodil Y Pham-Thi AT 《Journal of plant physiology》2008,165(5):514-521
Two cDNAs of the enzyme glutathione reductase (GR; EC 1.6.4.2) encoding a dual-targeted isoform (dtGR) and a cytosolic isoform (cGR), were cloned from leaves of common bean (Phaseolus vulgaris L.). Moderate drought stress (Psi w=-1.5MPa) followed by re-watering was applied to common bean cultivars, one tolerant to drought (IPA), the other susceptible (Carioca) and to cowpea (Vigna unguiculata L. Walp) cultivars, one tolerant to drought (EPACE-1), and the other susceptible (1183). mRNA levels were much higher for PvcGR than for PvdtGR in all cases. Moderate drought stress induced an up-regulation of the expression of PvcGR in the susceptible cultivars. On the contrary, PvdtGR expression decreased. In the tolerant cowpea EPACE-1, GR gene expression remained stable under drought. During recovery from drought, an up-regulation of the two GR isoforms occurred, with a peak at 6-10h after re-hydration. This suggests that moderate drought stress may lead to a hardening process and acclimation tolerance. The role of GR isoforms in plant tolerance and capacity to recover from drought stress is discussed. 相似文献
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Torres-Franklin ML Gigon A de Melo DF Zuily-Fodil Y Pham-Thi AT 《Physiologia plantarum》2007,131(2):201-210
Membranes are main targets of drought, and there is growing evidence for the involvement of membrane lipid in plant adaptation to such an environmental stress. Biosynthesis of the galactosylglycerolipids, monogalactosyl-diacylglycerol (MGDG) and digalactosyl-diacylglycerol (DGDG), which are the main components of chloroplast envelope and thylakoid membranes, could be important for plant tolerance to water deficit and for recovery after rehydration. In this study, galactolipid (GL) biosynthesis in cowpea ( Vigna unguiculata L. Walp) leaves was analysed during drought stress and subsequent rewatering. Comparison of two cowpea cutivars, one drought tolerant and the other drought susceptible submitted to moderate drought stress, revealed patterns associated with water-deficit tolerance: increase in DGDG leaf content, stimulation of DGDG biosynthesis in terms of 14 C-acetate incorporation and messenger accumulation corresponding to four genes coding for GL synthases ( MDG1 , MGD2 , DGD1 and DGD2 ). Similar to phosphate starvation, lack of water enhanced DGDG biosynthesis and it was hypothesized that the drought-induced DGDG accumulated in extrachloroplastic membranes, and thus contributes to plant tolerance to arid environments. 相似文献
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Maria-Lucia Torres-Franklin Anne Repellin Van-Biet Huynh Agnès d’Arcy-Lameta Yasmine Zuily-Fodil Anh-Thu Pham-Thi 《Environmental and Experimental Botany》2009,65(2-3):162-169
Membrane polyunsaturated fatty acids (PUFA) and particularly linolenic acid (18:3, LA) are known to be implicated in plant tolerance to low temperature. Their role in resistance to drought is much less investigated. In this work, three full-length cDNAs corresponding to omega-3 fatty acid desaturases: fad3 (endoplasmic reticulum), fad7 and fad8 (chloroplastic) were isolated from Vigna unguiculata leaves. Two cowpea cultivars, one drought-tolerant, EPACE-1, and one drought-susceptible, 1183, were compared in terms of fad isoform gene expression and leaf LA contents in plants submitted to water stress followed by rehydration. In EPACE-1, LA content in the main leaf polar lipids increased in response to mild water deficit. Severe water deficits induced a decrease in MGDG LA content while those of PC and DGDG continued to increase. Variations in FAD gene expression, matched those in LA contents. In 1183, LA contents decreased in all lipid classes in response to water stress, as did FAD3 and FAD8 gene expression levels. Rehydration after a moderate water stress induced stimulation mostly in FAD3 gene expression in both cvs. LA contents were equivalent to control levels in EPACE-1. In 1183, they were back to control levels in PC shortly after rehydration but remained low in galactolipids. These results suggested that omega-3 FAD activities were involved in the increase in leaf membrane unsaturation, in the drought tolerant plants whereas the sensitive plants lost PUFAs in response to the treatment. The significance of this discrepancy between the two cvs. in terms of adaptation to drought is discussed. 相似文献
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Satoshi Iuchi Kazuko Yamaguchi-Shinozaki Takeshi Urao Kazuo Shinozaki 《Journal of plant research》1996,109(4):415-424
Ten cDNAs for drought-inducible genes were isolated using differential screening of a cDNA library prepared from 10-hr dehydrated
cowpea plants,Vigna unguiculata (S. Iuchi, K. Yamaguchi-Shinozaki, T. Urao, T. Terao, K. Shinozaki; Plant Cell Physiology, 1996 in press). Two of the cDNA
clones, designated CPRD12 and CPRD46, were sequenced and characterized. The CPRD12 and CPRD46 cDNAs encode putative proteins
related to nonmetallo-short-chain alcohol dehydrogenase (CPRD12) and chloroplastic lipoxygenase (CPRD46). Northern blot analysis
revealed that these genes are induced by high-salinity stress and exogenous abscisic acid, but not by cold stress. The CPRD46
gene is also responsive to heat stress and methyl jasmonate and salicylic acid. Genomic Southern blot analysis suggested that
CPRD12 constitutes a small gene family, but that CPRD46 is a single copy gene. We discuss the possible functions of these
two CPRD gene products under drought stress. 相似文献
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Oxygen-free radicals are thought to play an essential role in senescence. Therefore, the expression patterns of the small
gene family encoding the H2O2 scavenging enzymes ascorbate peroxidase (APX; EC 1.11.1.11) were analyzed during senescence of Arabidopsis thaliana (L.) Heinh. Applying real-time RT-PCR, the mRNA levels were quantified for three cytosolic (APX1, APX2, APX6), two chloroplastic
types (stromal sAPX, thylakoid tAPX), and three microsomal (APX3, APX4, APX5) isoforms identified in the genome of Arabidopsis. The genes of chloroplastic thylakoid-bound tAPX and the microsomal APX4 exhibit a strong age-related decrease of mRNA level
in leaves derived from one rosette as well as in leaves derived from plants of different ages. In contrast to the tAPX, the
mRNA of sAPX was only reduced in old leaves of old plants. The microsomal APX3 and APX5, and the cytosolic APX1, APX2, and
APX6 did not show remarkable age-related changes in mRNA levels. The data show that expression of the individual APX genes
is differentially regulated during senescence indicating possible functional specialization of respective isoenzymes. The
hydrogen peroxide levels seem to be controlled very precisely in different cell compartments during plant development. 相似文献
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Narendra S Venkataramani S Shen G Wang J Pasapula V Lin Y Kornyeyev D Holaday AS Zhang H 《Journal of experimental botany》2006,57(12):3033-3042
Ascorbate peroxidase (APX) exists as several isoforms that are found in various compartments in plant cells. The cytosolic and chloroplast APXs appear to play important roles in antioxidation metabolism in plant cells, yet the function of peroxisomal APX is not well studied. In this study, the localization of a putative peroxisomal membrane-bound ascorbate peroxidase, APX3 from Arabidopsis, was confirmed by studying the green fluorescent protein (GFP)-APX3 fusion protein in transgenic plants. GFP-APX3 was found to co-localize with a reporter protein that was targeted to peroxisomes by the peroxisomal targeting signal 1. The function of APX3 in Arabidopsis was investigated by analysing an APX3 knockout mutant under normal and several stress conditions. It was found that loss of function in APX3 does not affect Arabidopsis growth and development, suggesting that APX3 may not be an important antioxidant enzyme in Arabidopsis, at least under the conditions that were tested, or the function of APX3 could be compensated by other antioxidant enzymes in plant cells. 相似文献
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