高原肺水肿患者血浆ET-1、TXB2及6-Keto-PGF1a水平的变化

内皮素 (ＥＴ 1 )、血栓素Ａ2 (ＴｘＡ2 )、前列环素 (ＰＧＩ2 )与心、肺疾病发病的关系受到了广泛重视 ,但在高原肺水肿 (ＨＡＰＥ)发病中的作用国内报道尚少。为此本研究测定了 8例ＨＡＰＥ患者的血浆ＥＴ 1含量以及ＴｘＡ2 、ＰＧＩ2 代谢产物血栓素Ｂ2 (ＴｘＢ2 )6 酮 前列腺素Ｆ1ａ(6 ｋｅｔｏ ＰＧＦ1ａ)含量变化 ,探讨其在ＨＡＰＥ发病中的变化及意义。1　材料与方法(1 )受试对象　 8例ＨＡＰＥ患者均来自海拔 430 0ｍ以上边防哨卡和新藏公路沿线民工 ,汉族男性 ,年龄 2 0～ 45岁 ,平均30 6岁 ,平原出生 ,进驻高原 3～ 5ｄ发病…     

双链杂交分子“胰岛素－类胰岛素生长因子－I

以去Ｂ链Ｃ端八肽胰岛素（ＤＯＩ）和化学合成的ＩＧＦ－Ｉ的２２～２９（８肽）及２２－３２（１１肽）为底物,用酶促半合成方法制备了杂交分子“胰岛素－类胰岛素生长因子－Ｉ,Ｉｎｓ／ＩＧＦ－Ｉ（８）和Ｉｎｓ／ＩＧＦ－Ｉ（１１）。研究了它们的胰岛素生物活性,结果表明,猪胰岛素Ｂ链Ｃ端Ｂ２７的Ｔｈｒ被Ａｓｎ取代,Ｂ３０的Ａｌａ被Ｔｈｒ取代同时Ｂ２５～Ｂ２６及Ｂ２８－Ｂ２ｋ９氨基酸顺序颠倒以及在Ｂ链Ｃ末端延长３肽（Ｇｌｙ－Ｔｙｒ－Ｇｌｙ）都不影响胰岛素的生物活力。     

用酵母双杂交系统研究胰岛素样生长因子—1突变体与其结合蛋白 …

为研究胰岛素样生长因子－１（ＩＧＦ１）及其突变体与ＩＧＦ结合蛋白－３（ＩＧＦＢＰ３）的相互作用,针对ＩＧＦ１的第３、４、１５、１６位氨基酸残基,采用定点突变的方法构建了「Ｙ１５Ｌ１６」ＩＧＦ１和「Ｑ３Ａ４Ｙ１５Ｌ１６」ＩＧＦ１。然后分别将ＩＧＦ１／ＩＧＦ１突变体和ＩＧＦＢＰ３ ｃＤＮＡ克隆至酵母表达载体ｐＧＢＴ９和ｐＡＣＴ２中,利用用酵母双杂交技术检测ＩＧＦ１／ＩＧＦ１突变体和ＩＧＦＢＰ３之间的相     

双链杂交分子“胰岛素—类胰岛素生长因子—I”

以去Ｂ链Ｃ端八肽胰岛素和化学合成的ＩＧＦ－Ｉ的２２－２９及２２－３２为底物,用酶促半合成方法制备了杂交分子“胰岛素－类胰岛素茵子－Ｉ”,Ｉｎｓ／ＩＧＦ－Ｉ和Ｉｎｓ－ＩＧＦ－Ｉ。研究了它们的胰岛中生物活性。结果表明,猪胰岛素Ｂ链Ｃ端Ｂ２７的Ｔｈｒ被Ａｓｎ取代,Ｂ３０的Ａｌａ被Ａｌａ被Ｔｈｒ取代同时Ｂ２５－Ｂ２６及Ｂ２８－Ｂ２９氨基酸顺序颠紧及在Ｂ链Ｃ末端延长３肽都不影响胰岛素的生物活力。     

大鼠酰胺化酶的信号肽引导的昆虫细胞表达外泌系统

将大鼠酰胺化酶的信号肽及前导肽编码序列引入昆虫核多角体病毒转移表达载体,构建ＰＡＢＣｈＧＲＦ（Ｇｌｙ）、ＰＡＢＣＩＧＦＩ融合基因的昆虫细胞分泌表达质粒ｐＢａｃＰＡＧ２、ｐＢａｃＰＡＩ,并与经修饰的银纹夜蛾核多角体病毒ＢａｃＰＡＫ６线性化ＤＮＡ共转染秋粘虫细胞Ｓｆ２１,通过同源重组、筛选和鉴定,得到它们的重组病毒ＢａｃＰＡＧ、ＢａｃＰＡＩ。将重组病毒感染Ｓｆ２１细胞,ＰＡＢＣｈＧＲＦ（Ｇｌｙ）和ＰＡＢＣＩＧＦＩ均得到有效外泌表达,表达产物通过ＩｇＧＳｅｐｈａｒｏｓｅ柱可获得快速纯化。     

人胰岛素样生长因子Ⅰ在大肠杆菌中的表达研究

胰岛素样生长因子Ⅰ（ＩＧＦ－Ⅰ）是一种多功能细胞增殖调控因子,它对许多疾病有较好的治疗作用．为了获得大量的ＩＧＦ－Ⅰ产品,在测定了ＩＧＦ－Ⅰ全长序列的基础上,构建了ＩＧＦ－Ⅰ表达载体ｐＢＶＩＧＦ,经热诱导表达后,ＳＤＳ－ＰＡＧＥ分析表明：含有重组表达质粒的菌株可表达出７．６ｋＤ的蛋白．研究了不同菌株对ＩＧＦ－Ⅰ表达的影响．ＩＧＦ－Ⅰ的表达水平可达１５ｍｇ／Ｌ．重组蛋白主要以包涵体形式存在,进行了初步纯化和复性研究,Ｗｅｓｔｅｒｎ印迹表明重组蛋白具有ＩＧＦ－Ⅰ的抗原性．并初步建立了ＩＧＦ－Ⅰ生物活性测定方法．     

大疱性类天疱疮IgG型抗基底膜带抗体的靶抗原初步分析

目的　研究大疱性类天疱疮 Ｉｇ Ｇ 型抗基底膜带抗体的靶抗原。方法　应用 Ｄ Ｉ Ｆ与 Ｉ Ｉ Ｆ检测的 Ｉｇ Ｇ型抗体阳性 Ｂ Ｐ 血清进行 Ｗ ｅｓｔｅｒｎ 免疫印迹分析。结果　初步研究发现 Ｉｇ Ｇ 阳性的 Ｂ Ｐ 血清能结合抗原１８０ Ｋ Ｄ,１６０ Ｋ Ｄ 以及 １０５ Ｋ Ｄ 与 ７７ Ｋ Ｄ 的多肽。结论　 Ｉｇ Ｇ 型 Ｂ Ｐ自身抗体可能靶向基底膜带不同层出现的不同抗原并提示该病的发生可能涉及不同的免疫机制。     

尖吻蝮蛇毒内一种新抗凝血因子ACF II 的纯化及特性

利用阴离子交换层析、凝胶过滤及阳离子交换层析三步方法, 从皖南尖吻蝮蛇毒中分离纯化到一个新的抗凝血因子ＡＣＦＩＩ（ａｎｔｉｃｏａｇｕｌａｔｉｏｎ ｆａｃｔｏｒＩＩ） 。纯化的ＡＣＦＩＩ在ＰＡＧＥ、ＳＤＳＰＡＧＥ和ＩＥＦＰＡＧＥ图谱上均呈单一区带。ＡＣＦＩＩ由两条分子量为１４．６ ｋＤ 的肽链通过二硫键连接在一起, 其等电点为７．０。ＡＣＦＩＩ具有显著的抗凝血活性, 在体外延长ＰＰＴ 时间的最终浓度为０ ．４ ｍｇ／Ｌ。ＡＣＦＩＩ不具有类凝血酶活性、磷脂酶Ａ２ 活性和纤溶活性,也没有出血活性和毒性, 是一种潜在的高效抗凝药物。     

多叶猴耳环──猴耳环属一新种

多叶猴耳环──猴耳环属一新种文和群（广西植物研究所分类研究室,桂林５４１００６）ＰＩＴＨＥＣＥＬＬＯＢＩＵＭＭＵＬＴＩＦＯＬＩＯＬＡＴＵＭ－ＡＮＥＷＳＰＥＣＩＥＳＯＦＰＩＴＨＥＣＥＬＬＯＢＩＵＭＦＲＯＭＧＵＡＮＧＸＩ,ＣＨＩＮＡＷｅｎＨｅｑｕｎ（Ｇｕ...     

高效融合表达中载体蛋白对hIGF－Ⅰ免疫原性结构形成的影响

利用人工全合成人胰岛素样生长因子工（ｈＩＧＦ－Ｉ）基因,构建了分别以β－半乳糖苷醇（β－ｇａｌ）的三种即含５９０、３１０、２８０个氨基酸序列片段和一种以ＰｒｏｔｅｉｎＡ的Ｂ、Ｃ结构域（ＰＡＢＣ）为载体蛋白的融合型表达质粒,并在大肠杆菌中高效表达了以上各种ｈＩＧＦ－Ｉ的融合蛋白产物。通过对羟胺裂解前后的ｈＩＧＦ－Ｉ产物进行放射免疫结合测定分析,结果显示以β－Ｇａｌ６９０、３１０、２８０为载体蛋白,均严重影响与其融合的ｈＩＧＦ－Ｉ的免疫原性结构形成,但在ＰＡＢＣ－ｈＩＧＦ－Ｉ融合蛋白中,载体蛋白ＰＡＢＣ无明显的影响作用。这表明在高融合表达低分子量蛋白或肽段中,需选择适宜的载体蛋白,以利于目的产物的功能性结构形成。     

Investigation of the mechanism of temperature sensitivity of the electroreceptors of ampullae of lorenzini

In experiments on Black Sea skates (Raja clavata), the potential of the receptor epithelium of the ampullae of Lorenzini and spike activity of single nerve fibers connected to them were investigated during electrical and temperature stimulation. Usually the potential within the canal was between 0 and –2 mV, and the input resistance of the ampulla 250–400 k. Heating of the region of the receptor epithelium was accompanied by a negative wave of potential, an increase in input resistance, and inhibition of spike activity. With worsening of the animal's condition the transepithelial potential became positive (up to +10 mV) but the input resistance of the ampulla during stimulation with a positive current was nonlinear in some cases: a regenerative spike of positive polarity appeared in the channel. During heating, the spike response was sometimes reversed in sign. It is suggested that fluctuations of the transepithelial potential and spike responses to temperature stimulation reflect changes in the potential difference on the basal membrane of the receptor cells, which is described by a relationship of the Nernst's or Goldman's equation type.I. P. Pavlov Institute of Physiology, Academy of Sciences of the USSR, Leningrad. I. M. Sechenov, Institute of Evolutionary Physiology and Biochemistry, Academy of Sciences of the USSR, Leningrad. Pacific Institute of Oceanology, Far Eastern Scientific Center, Academy of Sciences of the USSR, Vladivostok. Translated from Neirofiziologiya, Vol. 12, No. 1, pp. 67–74, January–February, 1980.     

Principles of organization and evolution of systems of regulation of functions

Evolution of living organisms is closely connected with evolution of structure of the system of regulations and its mechanisms. The functional ground of regulations is chemical signalization. As early as in unicellular organisms there is a set of signal mechanisms providing their life activity and orientation in space and time. Subsequent evolution of ways of chemical signalization followed the way of development of delivery pathways of chemical signal and development of mechanisms of its regulation. The mechanism of chemical regulation of the signal interaction is discussed by the example of the specialized system of transduction of signal from neuron to neuron, of effect of hormone on the epithelial cell and modulation of this effect. These mechanisms are considered as the most important ways of the fine and precise adaptation of chemical signalization underlying functioning of physiological systems and organs of the living organism