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1.
Blood biochemical polymorphisms as markers for genetic characteristics of wild Spanish and domestic rabbits 总被引:1,自引:0,他引:1
Seventeen blood proteins were studied in a sample of 412 Spanish wild rabbits and in 598 domestic rabbits belonging to various breeds. The wild rabbit populations showed a high level of genetic polymorphism. Six loci were monomorphic, while the remaining ten loci were segregating for at least two alleles. Two of the loci that were polymorphic in the wild rabbits were monomorphic in the domestic ones. Wright's inbreeding coefficient in the total Spanish wild rabbit population was F=5.66, indicating subdivision of the total population. Inbreeding coefficients, estimated by Kidd et al.'s method (Anim. Blood Grps, Biochem. Genet. 11: 21–38), differed significantly from zero, being 15.62%, in wild rabbits and 6–12% in domestic breeds, indicating consanguinity.Genetic distances between wild rabbit populations showed that factors other than geographic distance (e.g., bottlenecks, barriers such as rivers, mountains, etc.) may explain the result that a northern population forms a cluster with two central populations whereas the northeastern populations form a different cluster with another central population. Populations of the first cluster are more closely related to the captive populations than others.There are three population clusters of domestic rabbits, namely (1) New Zealand White and a hybrid combination; (2) Spanish Common, Butterfly, Burgundy, and Californian; and (3) Spanish Giant. 相似文献
2.
Summary. Genetic polymorphism of serum albumin was demonstrated by isoelectric focusing in wild rabbit populations from Portugal and England. Gene frequencies were estimated to be (1) ALB*1 = 0.47, ALB*2 = 0.49, ALB *3 = 0.04, in Portugal, and (2) ALB*1 = 0.60 and ALB*2 = 0.40, in England. One hundred Portuguese domestic rabbits of mixed breeds were all of ALB 1 type. 相似文献
3.
兔黑素皮质素受体4(MC4R)基因多态性及其与体重及屠体性状的关联研究 总被引:3,自引:0,他引:3
以哈尔滨白兔、天府黑兔、比利时兔、齐卡巨型兔以及加利福尼亚兔5个肉兔品种共220个个体为研究对象, 采用PCR-SSCP技术和克隆测序技术进行SNPs检测和基因型的分析。结果发现兔MC4R基因237位发生转换突变(A→G), 5个品种内的AA基因型和A等位基因频率均分别高于AG基因型和G等位基因, 而哈尔滨白兔、比利时兔以及齐卡巨型兔3个品种兔的AG基因型频率和G等位基因频率明显高于天府黑兔和加利福尼亚兔。单位点基因型与屠体性状、饲料转化率以及熟肉率的最小二乘分析表明; AG基因型与兔的体重、全净膛重以及饲料转化率有显著的关系(P<0.05), 而与熟肉率的关系不显著(P>0.05)。结果表明, MC4R基因可以作为影响和控制兔体重及屠体性状的候选基因。 相似文献
4.
Carneiro M Afonso S Geraldes A Garreau H Bolet G Boucher S Tircazes A Queney G Nachman MW Ferrand N 《Molecular biology and evolution》2011,28(6):1801-1816
Understanding the genetic structure of domestic species provides a window into the process of domestication and motivates the design of studies aimed at making links between genotype and phenotype. Rabbits exhibit exceptional phenotypic diversity, are of great commercial value, and serve as important animal models in biomedical research. Here, we provide the first comprehensive survey of nucleotide polymorphism and linkage disequilibrium (LD) within and among rabbit breeds. We resequenced 16 genomic regions in population samples of both wild and domestic rabbits and additional 35 fragments in 150 rabbits representing six commonly used breeds. Patterns of genetic variation suggest a single origin of domestication in wild populations from France, supporting historical records that place rabbit domestication in French monasteries. Levels of nucleotide diversity both within and among breeds were ~0.2%, but only 60% of the diversity present in wild populations from France was captured by domestic rabbits. Despite the recent origin of most breeds, levels of population differentiation were high (F(ST) = 17.9%), but the majority of polymorphisms were shared and thus transferable among breeds. Coalescent simulations suggest that domestication began with a small founding population of less than 1,200 individuals. Taking into account the complex demographic history of domestication with two successive bottlenecks, two loci showed deviations that were consistent with artificial selection, including GPC4, which is known to be associated with growth rates in humans. Levels of diversity were not significantly different between autosomal and X-linked loci, providing no evidence for differential contributions of males and females to the domesticated gene pool. The structure of LD differed substantially within and among breeds. Within breeds, LD extends over large genomic distances. Markers separated by 400 kb typically showed r(2) higher than 0.2, and some LD extended up to 3,200 kb. Much less LD was found among breeds. This advantageous LD structure holds great promise for reducing the interval of association in future mapping studies. 相似文献
5.
6.
R Ramchandani X Shen C L Elmsley W T Ambrosius S J Gunst R S Tepper 《Journal of applied physiology》2000,89(4):1310-1316
Our laboratory has previously demonstrated that maximal bronchoconstriction produces a greater degree of airway narrowing in immature than in mature rabbit lungs (33). To determine whether these maturational differences could be related to airway structure, we compared the fraction of the airway wall occupied by airway smooth muscle (ASM) and cartilage, the proportion of wall area internal to ASM, and the number of alveolar attachments to the airways, from mature and immature (6-mo- and 4-wk-old, respectively) rabbit lungs that were formalin fixed at total lung capacity. The results demonstrate that the airway walls of immature rabbits had a greater percentage of smooth muscle, a lower percentage of cartilage, and fewer alveolar attachments compared with mature rabbit airways; however, we did not find maturational differences in the airway wall thickness relative to airway size. We conclude that structural differences in the airway wall may contribute to the greater airway narrowing observed in immature rabbits during bronchoconstriction. 相似文献
7.
A. M. Almeida A. Campos R. Francisco S. Van Harten L. A. Cardoso A. V. Coelho 《Animal genetics》2010,41(3):260-272
The study of changes within the key agents regulating metabolism during genetic upgrading because of selection can contribute to an improved understanding of genomic and physiological relationships. This may lead to increased efficiencies in animal production. These changes, regarding energy and protein metabolic saving mechanisms, can be highlighted during food restriction periods. In this study, a 20% weight reduction was induced in two rabbit breeds: New Zealand white, a selected meat producer (Oryctolagus cuniculus cuniculus), and Iberian wild rabbit (Oryctolagus cuniculus algirus), with the aim of determining differential protein expression in the gastrocnemius muscle within control (ad libitum) and restricted diet experimental animal groups, using techniques of two‐dimensional gel electrophoresis and peptide mass fingerprinting. Results show that l ‐lactate dehydrogenase, adenylate kinase, β enolase and α enolase, fructose bisphosphate aldolase A and glyceraldehyde 3‐phosphate dehydrogenase, which are enzymes involved in energy metabolism, are differentially expressed in restricted diet experimental animal groups. These enzymes are available to be further tested as relevant biomarkers of weight loss and putative objects of manipulation as a selection tool towards increasing tolerance to weight loss. Similar reasoning could be applied to 2D gel electrophoresis spots corresponding to the important structural proteins tropomyosin β chain and troponin I. Finally, a spot identified as mitochondrial import stimulation factor seems of special interest as a marker of undernutrition, and it may be the object of further studies aiming to better understand its physiological role. 相似文献
8.
B Lund A von der Decken 《Comparative biochemistry and physiology. B, Comparative biochemistry》1984,78(2):343-350
Skeletal muscle was dissected from the forelimbs of animals and stored at -80 degrees C. Protein synthesis activity was determined in cell-free systems. Incorporation of amino acids into protein was expressed per milligramme of ribosomal RNA, per gramme wet weight of skeletal muscle and per milligramme of DNA. Species related differences in activity were observed in skeletal muscle obtained from pig, horse, lamb, sheep, calf and young steer. Intraspecies differences were seen in cattle of different breeds. The protein composition of the muscle tissue showed quantitative differences between the species. Using biopsy specimens, the system could be used to monitor the protein nutrition status of animals and the production of animal proteins. 相似文献
9.
Abundances of the parasitic nematodes Trichostrongylus retortaeformis and Passalurus ambiguus, and 8 Eimeria species were estimated by fecal egg and oocyst output in 12 discrete free-ranging populations of wild rabbits (Oryctolagus cuniculus) in southwestern Australia. Comparisons of parasite egg and oocyst counts were made between those rabbits known to have survived at least 2 mo after fecal samples were collected and those rabbits that did not survive. There were significant negative relationships between parasite egg and oocyst counts and survival when all age groups and collection periods were pooled for several species of coccidia and for T. retortaeformis. However, when the same comparisons were made within rabbit age groups and within collection periods, there were very few significant differences even where sample sizes were quite large. The differences indicated by the pooled analysis for coccidia were most likely due to an uneven host age distribution with respect to survival, combined with an uneven distribution of the oocyst counts with rabbit age. The result for T. retortaeformis was similarly affected but by a seasonal pattern. Parasitism by nematodes and coccidia did not appear to be an important mortality factor in these rabbit populations, at least at the range of host densities we examined. This suggests that other factors must have been responsible for the observed pattern of density-dependent regulation in these rabbits. 相似文献
10.
The ontogeny of haemolytic complement in rabbit serum and the genetic differences of the activity in five strains of adult rabbits were investigated by single radial haemolysis in gel and a microtiter method with purified complement components and the appropriate haemolytic intermediate cells. The haemolytic complement activity was found as early as the 15th day of foetal life, and increased with age reaching approximately adult level by the 120th day of life. Marked strain differences in both total haemolytic activity and C3 levels of adult rabbit sera were observed. The repeatability of haemolytic activity for an individual serum taken at different times was higher than that for C3 level was observed. An inherited complement deficiency, due to the lack of C6, was found in a strain of Angora rabbits. The genetic studies confirmed that this complement defect was transmitted as an autosomal recessive trait. 相似文献
11.
Little information is available on the role of genetic factors and heredity in normal ligament behaviour and their ability to heal. Assessing these factors is challenging because of the lack of suitable animal models. Therefore, the purpose of this study was to develop a porcine model in order to evaluate and compare the biomechanical differences of normal medial collateral ligaments (MCLs) between Yorkshire (YK) and red Duroc (RD) breeds. It was hypothesized that biomechanical differences would not exist between normal YK and RD MCLs. Comparisons between porcine and human MCL were also made. A biomechanical testing apparatus and protocol specific to pig MCL were developed. Ligaments were subjected to cyclic and static creep tests and then elongated to failure. Pig MCL morphology, geometry, and low- and high-load mechanical behaviour were assessed. The custom-designed apparatus and protocol were sufficiently sensitive to detect mechanical property differences between breeds as well as inter-leg differences. The results reveal that porcine MCL is comparable in both shape and size to human MCL and exhibits similar structural and material failure properties, thus making it a feasible model. Comparisons between RD and YK breeds revealed that age-matched RD pigs weigh more, have larger MCL cross-sectional area, and have lower MCL failure stress than YK pigs. The effect of weight may have influenced MCL geometrical and biomechanical properties, and consequently, the differences observed may be due to breed type and/or animal weight. In conclusion, the pig serves as a suitable large animal model for genetic-related connective tissue studies. 相似文献
12.
The activities of several enzymes functioning in different areas of fuel catabolism were measured under standardized conditions, using crude homogenates of sartorius and ventricular muscle from outbred guinea-pigs and rabbits indigenous to high or low altitude. The activities of sartorius and myocardium were found to reflect the metabolic patterns known to be associated with white and red muscle. Both species had right ventricular hypertrophy at high altitude. The enzyme activities in the high altitude guinea-pig were not significantly different from those in the sea level animals. In the high altitude rabbit, compared with the low altitude rabbit, the activities of glyceraldehyde-3-phosphate deydrogenase and phosphofructokinase were greater in both the sartorius and myocardium. In addition, mitochondrial glycerol-3-phosphate dehydrogenase activity was greater in the sartorius at high altitude, while aspartate aminotransferase and beta-hydroxyacylcoenzyme A dehydrogenase activities were greater in the myocardium at high altitude. Succinate dehydrogenase activity was comparable at the two altitudes for both tissues. There was a greater proportion of skeletal muscle type lactate dehydrogenase in the high altitude rabbit myocardium but no difference was found with the guinea-pig. 相似文献
13.
W F Colebrook J L Black G H Brown J B Donnelly 《Australian journal of biological sciences》1988,41(2):201-214
The relative importance of cell number and cell size in determining the mass of 16 organs and tissues in mature rams of six different breeds was studied through estimation of organ deoxyribonucleic acid (DNA) content. The mean fleece-free empty body weight (FFEBW) ranged from 54.6 +/- 0.3 kg for Camden Park Merinos to 76.7 +/- 1.6 kg for Strong Wool Merinos. For all organs, mass increased with FFEBW, but the relationship was significant across all sheep for only eight organs (blood, kidney, liver, abomasum, vastus lateralis muscle, skin, perirenal fat and triceps muscle). There were significant differences between breeds in the mass of 11 organs. With four (heart, rumen reticulum, small intestine and testicular fat) this difference was independent of breed differences in FFEBW, whereas with another four (kidney, abomasum, vastus lateralis muscle and skin), it was closely related to FFEBW. Breed differences in the mass of the remaining three organs (blood, liver and perirenal fat) were partly related to FFEBW and partly breed specific. Blood mass increased with FFEBW across all animals, but, within a breed, it declined as FFEBW increased. The increase in the mass of perirenal fat with FFEBW was significantly greater within a breed than between breeds. Cell number increased significantly with the mass of all organs except blood and brain. There were between-breed differences in the number of cells in seven organs (liver, heart, rumen reticulum, abomasum, small intestine, vastus lateralis muscle and skin), which, except for heart, were attributable to between-breed differences in organ mass. With heart, the increase in cell number with organ mass within a breed was greater than across all breeds. Cell size was significantly related to organ mass only with vastus lateralis muscle, spleen, perirenal fat and liver. The relationship for vastus lateralis muscle and spleen was negative, indicating that cells were smaller in larger organs. There were differences between breeds in cell size for heart, vastus lateralis and triceps muscles. These differences for heart and triceps muscle were breed specific, whereas for vastus lateralis muscle it was attributed to breed differences in organ weight. There was a 30-fold range in mean cell size across organs, with adipose tissue having the largest cells, muscle tissue intermediate and visceral tissues the smallest. In general, organ mass is positively related to FFEBW. Cell number, not cell size, is largely responsible for differences in organ mass between mature sheep of different breeds. 相似文献
14.
L Andersson S Bengtsson U Hellman I K?llman C Ranje 《Animal blood groups and biochemical genetics》1985,16(1):41-50
Previous studies on the amino acid sequences and on the amino acid composition of peptides revealed genetic polymorphism both of the haemoglobin alpha-chain (Hb alpha) and beta-chain (Hb beta) in rabbits. In this study, rabbit haemolysates were analysed by isoelectric focusing in a narrow pH range (6.7-7.7) and by reversed-phase chromatography. Two variants were found for both Hb alpha and Hb beta. The two methods detected the same variants in this material. Inheritance data were consistent with the hypothesis that the observed Hb alpha and Hb beta variants were each controlled by two codominant, autosomal alleles. Haemoglobin polymorphism appears to be frequent in domestic rabbits since both variants of each chain were observed in all the three breeds studied. 相似文献
15.
The ontogeny of haemolytic complement in rabbit serum and the genetic differences of the activity in five strains of adult rabbits were investigated by single radial haemolysis in gel and a microtiter method with purified complement components and the appropriate haemolytic intermediate cells. The haemolytic complement activity was found as early as the 15th day of foetal life, and increased with age reaching approximately adult level by the 120th day of life. Marked strain differences in both total haemolytic activity and C3 levels of adult rabbit sera were observed. The repeatability of haemolytic activity for an individual serum taken at different times was higher than that for C3 levels and no significant correlation between haemolytic activity and C3 level was observed. An inherited complement deficiency, due to the lack of C6, was found in a strain of Angora rabbits. The genetic studies confirmed that this complement defect was transmitted as an autosomal recessive trait. 相似文献
16.
兔成纤维细胞生长因子5(FGF5)基因SNP及其与产毛量的相关分析 总被引:5,自引:1,他引:5
通过PCR产物直接测序的方法, 对荥经长毛兔、天府黑兔以及加利福尼亚兔的FGF5基因外显子1和外显子3进行单核苷酸多态性分析。在外显子1的217位(位点A)检测到由TCT三碱基插入引起的移码突变, 在外显子3的59位(位点B)和3位(位点C)分别发生了错义突变由T→C和同义突变由T→C。通过计算发现各位点不同的基因型和等位基因频率在3个兔品种中存在较大的差异, 位点A、B在长毛兔和肉兔中均有各自的优势基因型和等位基因。各位点基因型与产毛量的最小二乘分析表明, 位点A各基因型的个体在产毛量上差异不显著(P>0.05), 位点B各基因型个体产毛量的差异极显著(P<0.01), 位点C各基因型个体产毛量的差异显著(P<0.05)。初步推断FGF5基因可能是影响长毛兔产毛量潜在的主效基因或者与主效基因连锁, 可作为长毛兔产毛性状连锁分析的候选遗传标记。 相似文献
17.
I Syrovy 《Physiologia Bohemoslovaca》1978,27(5):445-448
1. Ca2+-ATPase of myosin and electrophoretic pattern of light chains of myosin were investigated in cardiac muscles of 22-day-old rabbit embryos, new-born and adult rabbits. 2. Ca2+-ATPase activity was found to decrease during development and in contrast to that of adult rabbit, cardiac myosin prepared from 22-day-old embryos, is stable on exposure to pH 9.5. 3. Myosin from the cardiac muscle of rabbit embryos reveals light chains of both fast and slow types, that from adult animals, however, reveals light chains of the slow type only. 4. These studies suggest that unlike the cardiac muscle of adult rabbit, cardiac muscle of rabbit embryos contains both fast and slow types of myosin. 相似文献
18.
V. J. Kontsiotis D. E. Bakaloudis P. Xofis N. Konstantaras N. Petrakis A. Tsiompanoudis 《Ecological Research》2013,28(2):317-325
The European rabbit (Oryctolagus cuniculus) is a widely distributed mammal with an often contradictory ecological role, imposing the need for population management. Sound management requires an in-depth understanding of the complex species–habitat relationships. In this study, CART analysis was employed to identify the most important environmental and anthropogenic factors affecting the spatial distribution of wild rabbit on Lemnos Island, northeastern Aegean Sea in Greece. On Lemnos, this species is considered an agricultural pest due to its overabundance resulting from the long-term absence of viral diseases, limited predation pressure, and lack of effective management. The study was carried out during the summer of 2008 by surveying rabbit densities in 181 2 × 2-km squares. Seven environmental and 14 anthropogenic variables, measured at two spatial scales, were used as explanatory variables. Soil hardness was the most influential variable, dividing the island into two distinct areas, namely the rabbit-poor areas with hard rocky soils and the rabbit-rich areas where soft soils prevail. In the former, the presence of a sharp relief can lead to complete absence of the species, while a combination of gentle relief, low altitudes, and low presence of arable land can lead to moderate rabbit density. In the latter, human-caused disturbance can reduce the number of rabbits, while a high density of ecotones and streams and a high presence of riparian vegetation can increase population densities to its highest levels observed. Our findings can formulate a scientific basis for the development of an effective management strategy for its population control. 相似文献
19.
The macro and trace mineral composition of vertebrae and muscle in wild and cultured haddock Melanogrammus aeglefinus was determined. Results indicated that haddock deposit c . 10% higher amounts of mineral than salmonids. Based on distribution between the analysed tissues as well as concentration differences, the minerals in wild and cultured haddock were divided into four categories: (1) As, Mn, Na, S and Zn concentration did not show any significant ( P > 0·05) variation in both muscle and vertebrae; (2) Cr, Co, Mg, Mo and Si concentrations in wild and culture stocks muscle did not vary but vertebrae concentration showed significant differences ( P < 0·05); (3) Ca and Cu concentration varied in muscle while remaining the same in vertebrae; (4) Al, Ba, Fe, P, K and Sr concentration showed a significant difference in both muscle and vertebrae. Vertebrae P content of cultured haddock was lower than that of wild fish whereas muscle P content was relatively high in cultured fish. Vertebrae and muscle Fe content of cultured haddock was significantly higher than in wild fish. The Mg, K, Co and Cr concentrations in vertebrae were significantly higher in wild haddock than in cultured fish, whereas Mo and Al levels were low. These differences in trace element composition of wild and cultured haddock were probably related to differences in food source and therefore the mineral composition of the diet. The nutritional significance of these findings is discussed. 相似文献
20.
An experimental study of translocation success and habitat improvement in wild rabbits 总被引:1,自引:0,他引:1
The translocation of wild animals is a strategy frequently used in the conservation and management of natural populations. The aim of this study was to evaluate the impact of translocation (population supplementation) and habitat improvement on the abundance of European wild rabbit Oryctolagus cuniculus . We used eight open plots with different habitat treatments: two with increased shelter, two with increased food, two with increased shelter and food and two without habitat treatment (control plots). We translocated wild rabbits during 3 consecutive years, each year in four of the eight plots, with the remaining plots serving as control for the translocation treatment. Rabbit abundance (translocated plus native rabbits) was calculated by means of pellet counts, and the results were evaluated mainly by generalized linear mixed models. We found that rabbit abundance was determined primarily by habitat improvement. Rabbits were more abundant in treated than in control plots, and most abundant in the plots where food availability was increased. This effect persisted throughout the year. Translocation also increased abundance, but this effect was the strongest where shelter and food had also been improved and declined and disappeared after breeding. These findings suggest that the habitat is an important factor for rabbit abundance, with food availability being the prime factor regulating densities. Moreover, translocations on their own are only effective in the short term in situations in which factors limiting population growth (e.g. disease and predation) have not been corrected. Translocations should be carried out in conjunction with improvements in release habitat as rabbit densities will depend on the maximum carrying capacity of the habitat. 相似文献