低强度HeNe激光辐照人体外周血对淋巴细胞染色体影响的研究

本文通过低强度Ｈｅ－Ｎｅ激光以能量密度分别为１４．３１Ｊ／ｃｍ＾２（辐照５’）、２８．６２Ｊ／ｃｍ＾２（辐照１０）、５７．２４Ｊ／ｃｍ＾２（辐照２０）１１４．５２Ｊ／ｃｍ＾２（辐照４０）燠夫体外周血后,检测其淋染色畸变率（ＣＡ）,激光照射血样（能量密度由低到高）未照射血样ＣＡ分别平均为４．２９‰、３．９６‰、３．８１‰、３．５９０‰、４．１９‰、Ｘ＾检测无显著差异,说明低强度的Ｈｅ－Ｎｅ激光辐照人     

激光对尾草履虫分裂繁殖影响的研究

采用Ｈｅ－Ｎｅ激光辐照经单克隆培养的尾草履虫（Ｐａｒａｍｅｃｉｕｍｃａｕａｔｕｍ）无性繁殖系,观察并分析激光对尾草履虫分裂繁殖产生的影响,结果表明,在本实验条件下,当辐照的能量密度处于５０．９－１０１．９（Ｊ／ｃｍ＾２）范围内,激光对尾草履虫的分裂繁殖产生促进作用,当辐照的能量密度为１０１．９Ｊ／ｃｍ＾２时,草履虫分裂繁殖的个体平均数达最大值。草履虫对激光辐照有很大的耐受范围和很强的耐受能力。本实     

激光照射茶树花药培养及其过氧化物酶同工酶的分析

Ｈｅ－Ｎｅ和ＣＯ２激光对茶树花药培养的药裂率和出愈率的影响,既有促进作用,也有抑制作用。按出愈率和愈伤组织生长状况的序列：Ｈｅ－Ｎｅ：２０Ｊ／ｃｍ＾２〉ＣＫ〉Ｈｅ－Ｎｅ：３０Ｊ／ｃｍ＾２〉ＣＯ２：３５．４Ｊ／ｃｍ＾２。对Ｈｅ－Ｎｅ激光照射花药及其愈伤组织的过氧化物酶同工酶分析,主要表现在弱酶带的变化上,无规律差异。     

He—Ne激光照射离体鼠巨噬细胞对线粒体跨膜电势的影响

目的：研究Ｈｅ－Ｎｅ激光照射鼠巨噬细胞对线粒体跨膜电势的影响,及其与激光剂量的关系。方法：用亲脂性阳离子荧光染料Ｒｈｏｄａｍｉｎｅ１２３对鼠巨噬细胞线粒体作荧光标记,以不同的激光剂量照射,采用图像分析系统（ＩＡＳ）和荧光显微镜观察线粒体跨膜电势荧光强度的变化。结果：低功率Ｈｅ－Ｎｅ激光照射５,１０,１５ｍｉｎ,激光剂量分别为０．６４９,１．３８８和２．０８２Ｊ／ｃｍ＾２,巨噬细胞线粒体跨膜电势荧光     

不同剂量的He—Ne激光对Raji细胞膜表面SH的影响

本文以离体培养的Ｒａｊｉ细胞为材料,采用ＥＬＬＭＡＮ方法检测了不同剂量的Ｈｅ－Ｎｅ激光对Ｒａｊｉ细胞膜表面ＳＨ含量的影响。发现０．５Ｊ／ｃｍ＾２Ｈｅ－Ｎｅ激光能量可明显增加膜表面ＳＨ含量（Ｐ〈０．０５）。大于或小于此剂量的Ｈｅ－Ｎｅ激光对膜表面ＳＨ含量的影响均不明显（Ｐ〉０．０５）。提示０．５Ｊ／ｃｍ＾２的激光能量对膜有刺激作用。     

He—Ne激光照射对成纤维细胞内[Ca^2＋]i浓度影响的流式细胞计测定

目的：Ｈｅ－Ｎｅ激光照射治疗的机理不明,激光照射引起细胞内Ｃａ＾２＋水平变化,为治疗机理提供理论依据。方法：Ｈｅ－Ｎｅ激光照射引起鼠成纤维细胞Ｌ９２９内［Ｃａ＾２＋］ｉ的变化,用ＨＯ３４２对细胞ＤＮＡ活性染色,Ｆｌｕｏ－３ＡＭ对细胞内Ｃａ＾２＋染色,利用ＦＣＭ同时定量分析细胞ＤＮＡ和细胞内Ｃａ＾２＋的变化。结果：激光照射１５ｍｉｎ（光剂量１１．８１Ｊ／ｃｍ＾２后,ＦＣＭ分析可见ＤＮＡ分布直方图右移     

弱激光对脂质体介导的血管平滑肌细胞基因转染的影响

本研究采用阳离子脂质体介导外源基因转染体外培养的兔血管平滑肌细胞（ＳＭＣ）,在基因转染过程中给予激光照射,用细胞化学染色方法测定基因转染阳性率。结果显示：用５１０．６ｎｍ激光于基因转染前,以功率密度１ｍｗ／ｃｍ２,能量密度２、４、６Ｊ／ｃｍ２和５ｍＷ／ｃｍ２,４、６Ｊ／ｃｍ２;及１０ｍＷ／ｃｍ２,２Ｊ／ｃｍ２进行照射均能显著提高基因转染率（ｐ＜０．０５）;于基因转染后即刻以功率密度１ｍＷ／ｃｍ２、能量密度２Ｊ／ｃｍ２和５ｍＷ／ｃｍ２、６Ｊ／ｃｍ２照射也能提高基因转染率（ｐ＜０．０５）。而用６２７．８ｎｍ激光照射对基因转染率无显著影响     

低强度He-Ne激光照射人外周血淋巴细胞两组试验结果的分析

本文采用染色体畸变 (chromosomalaberration CA)试验和微核 (micronucleus)试验两种方法对低强度He Ne激光辐照育龄妇女外周血淋巴细胞。激光能量密度分别为 14.31J cm2 (辐照 5′)、2 8.6 2J cm2 (辐照 10′) ,5 7.2 4J cm2(辐照 2 0′) ,114.5 2J cm2 (辐照 40′)。照射血样后 ,染色体畸变试验检测其淋巴细胞染色体畸变率 ,激光照射及空白对照组 ,血样染色体畸变率分别为 4.2 9‰、3.96‰、3.81、3.5 9‰和 4.19‰ ,X2 检验无显著意义 (P >0 .0 5 )。阳性对照丝裂霉素MMC处理的血样淋巴细胞CA率平均为 14.41‰ ,明显高于激光照射和空白对照组 ,X2 检验有显著差异(P <0 .0 1)。微核试验检测结果 ,微核染色体分别为 1.0 2‰ ,1.17‰ ,1.18‰ ,1.31‰和 1.19‰对照 ,经统计分析激光照射各组与对照组微核率均在 2‰以下 (P >0 .0 5 ) ,属正常人体微核范围内。结果显示两组试验监测诱变均具有一致性。证明He Ne激光辐照人体细胞对染色体无致畸效应。且表明He Ne激光在治疗范围内应用安全、有效、不会对不体造成危害。     

510.6nm激光照射对兔血管平滑肌细胞增殖的影响

本文用５１０．６ｎｍ 波长激光以功率密度１、５、１０ ｍ Ｗ／ｃｍ ２ 和能量密度２、４、６Ｊ／ｃｍ ２ 照射体外培养的兔血管平滑肌细胞（ＳＭＣ）,通过３Ｈ－ ＴｄＲ掺入率和细胞生长曲线测定细胞增殖率。结果显示,上述激光照射量均能抑制细胞增殖率,其中以１０ｍ Ｗ／ｃｍ ２ 组的作用最为显著     

不同剂量的He—Ne激光对Raji细胞膜脂流动性的影响

本文采用荧光偏振法检测了不同剂量的Ｈｅ－Ｎｅ激光照射法对Ｒａｊｉ细胞膜流动性的影响,发现了０．０１Ｊ／ｃｍ＾２和０．５１Ｊ／ｃｍ＾２两剂量可使膜脂微粘度降低（Ｐ〈０．０５）。即其膜脂流动性升高;１．０Ｊ／ｃｍ＾２和２．０Ｊ／ｃｍ＾２的剂量脂微粘度无明显影响,即其膜流动性无明显化（Ｐ〉０．０５）,而６．０２Ｊ／ｃｍ＾２的剂量可使膜微粘度明显上升（Ｐ〈０．０５）。即其膜流动性明显下降。     

六种藓类植物茎的比较解剖学研究

通过对6种藓类植物,即褶叶青藓(Brachythecium salebrosum(Web.et Mohr.)B.S.G.)、湿地匐灯藓(Plagiomnium acutum(Lindb.)Kop.)、侧枝匐灯藓(Plagiomnium maximoviczii(Lindb.)Kop.)、大凤尾藓(Fissidensnobilis Griff.)、大羽藓(Thuidium cymbifolium(Doz.et Molk.)B.S.G.)和大灰藓(Hypnum plumaeforme Wils.)嫩茎和老茎的石蜡切片和显微观察发现,同一藓类植株的嫩茎和老茎,茎结构稳定,不同种藓类植物茎横切面具有不同特征.植物体茎横切面形状、表层细胞的层数、细胞大小和细胞壁厚薄、皮层细胞大小和形状、中轴的有无以及比例等特征可以作为藓类植物的分科分类依据之一.     

Cause of Senescence of Nine Sorts of Flowers

The levels of endogenous phytohormones and respiratory rate in nine sorts of flowers such as Cymbidium faberi Rolfe, Nopalxochia ackermannii Kunth and others were investigated both at full bloom and senescence and meanwhile the effect of exogenous phytohormones on prolonging the blossoms and promoting ethylene production were tested. There is a high content of endogenous ethylene in all the long-lived flowere, about 3–16 folds higer than the short-lived ones. There is a high level of ABA at full blooming flowers of short-lived flowers, in which there is no or only some cytokinins in it, but the ratio of CTK (6BA+zeatin)/ABA is smaller(l.7). The endogenous ABA reached a much higher level at senescence in all nine sorts of flowers, so it is reasonable to consider that it is ABA which plays an important role of regulation in controlling flower's senescence. There is a much higher level of GA3 and zeatin in the long-lived flowers which is not demonstrated in the shortlived ones. The respiratory rate is one of the factors controtling the longevity of flowers, but it does not play a decided role. Application of 6BA and zeatin prolongs distinctly orchid’s longevity, however exogenous IAA through the promotive action on ethylene production, evidently extends the longevity of the flowers of the Nopalxochia ackermannii Kunth.     

龙胆科药用植物化学成分的研究现状

龙胆科植物在我国的分布范围很广,且多数为药用植物,其多数种属的药用植物,至今其化学成分尚未被系统研究。综述了目前龙胆科药用植物的化学成分的研究现状及一般提取方法,对近年来发现的环烯醚萜及裂环烯醚萜类化合物进行了总结,为本科药用植物的更深入研究提供了参考。     

真菌类遗传学分析的知识结构教学

本文以认知结构理论为指导,讨论了真菌类遗传分析与高等动植物遗传分析的内在联系,认为利用这种内在联系进行教学可收到好的效果并说明了作者的具体教学过程。 Abstract:In the paper, the relationship between genetic analysis of Fungi and genetic analysis of high animal and plant was discussed.A good results were obtained when we adopted this method in the teaching.     

The effects of glutamine of the maintenance of embryogenic cultures of Cryptomeria japonica

Summary Embryogenic tissues of sugi (Cryptomeria japonica) were induced on a modified Campbell and Durzan (CD) medium containing 1 μM 2,4-dichlorophenoxyacetic acid (2,4-D) and 600 mg l⁻¹ glutamine, and subcultured in the medium of the same composition for over 1 yr. This resulted in a mixed culture of embryogenic and non-embryogenic cells. When embryogenic cells were isolated and cultured independently, their capacity to form embryogenic aggregates was lost. Thus, the non-embryogenic cells present within a mixed culture system were essential to the formation of embryogenic aggregates. When embryogenic tissues were isolated and cultured independently on a high glutamine-containing (2400 mg l⁻¹) medium, dry weights and endogenous levels of glutamine increased, and the tissue could generate a large number of embryogenic aggregates. Amino acid analysis of embryogenic and non-embryogenic cells from the maintenance culture indicated a higher level of glutamine was present in the latter. The high endogenous level of glutamine in the non-embryogenic portion of mixed cell masses may be the supplier of glutamine for maintaining the embryogenic property of the tissues.     

Specificity of action of piperidylcholine derivatives as substrates of cholinesterases of various origin

The review deals with study of enzymologic properties of a novel highly specific acetylcholinesterase substrate, N-(β-acetoxyethyl) piperidinium iodomethylate (“piperidylcholine”), and its 30 derivatives that were tested as effectors of cholinesterases of mammals and various species of Pacific squids. It was proven for the first time that responsible for specificity of action was structure of cyclic ammonium grouping of the alcohol part of molecule of the ester substrate. Analysis of specificity is performed based on enzymatic hydrolysis parameters—activity of catalytic center of cholinesterases and bimolecular constant of the reaction rate that are determined at optimal and low substrate concentrations. Among the specially synthesized group of thioester compounds there is revealed one more highly specific acetylcholinesterase substrate—N-(β-acetoxyethyl) piperidinium.     

医疗机构合理用药评价模型的构建研究

目的 针对医疗机构的合理用药水平进行评价研究。方法 根据医疗机构合理用药的具体要求,构建医疗机构合理用药评价指标体系,采用基于模糊群决策的方法和多指标评价分析法构建医疗机构合理用药评价模型。结果 构建了基于模糊群决策的医疗机构合理用药评价模型,并通过实例分析证明了评价模型的可行性。结论 建立的基于模糊群决策的医疗机构合理用药评价模型能够对医疗机构的合理用药水平进行科学评价,为提高医疗机构合理用药水平奠定基础。     

Photoregulation of seed germination of wild-type and of an aurea-mutant of tomato

Seed germination of an aurea mutant of tomato ( Lycopersicon esculentum Mill.) is promoted by continuous irradiation with red, far-red or long-wavelength far-red (758 nm) light as well as by cyclic irradiations (5 min red or 5 min far-red/25 min darkness). Far-red light applied immediately after each red does not change the germination behaviour. Seed germination of the isogenic wild-type, cv. UC-105, is promoted by continuous and cyclic red light while it is inhibited by continuous and cyclic far-red light and by continious 758 nm irradiation. Far-red irradiation reverses almost completely the promoting effect of red light. The promoting effect (in the aurea mutant) and the inhibitory effect (in the wild-type) of continuous far-red light do not show photon fluence rate dependency above 20 nmol m⁻² s⁻¹. It is concluded that phytochrome controls tomato seed germination throgh low energy responses in both the wild type and the au mutant. The promoting effect of continuous and cyclic far-red light in the au mutant can be attributed to a greater sensitivity to P_fr.