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1.
Yahiaoui S Igartua E Moralejo M Ramsay L Molina-Cano JL Ciudad FJ Lasa JM Gracia MP Casas AM 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2008,116(2):271-282
The pool of Western Mediterranean landraces has been under-utilised for barley breeding so far. The objectives of this study
were to assess genetic diversity in a core collection of inbred lines derived from Spanish barley landraces to establish its
relationship to barleys from other origins, and to correlate the distribution of diversity with geographical and climatic
factors. To this end, 64 SSR were used to evaluate the polymorphism among 225 barley (Hordeum vulgare ssp. vulgare) genotypes, comprising two-row and six-row types. These included 159 landraces from the Spanish barley core collection (SBCC)
plus 66 cultivars, mainly from European countries, as a reference set. Out of the 669 alleles generated, a large proportion
of them were unique to the six-row Spanish barleys. An analysis of molecular variance revealed a clear genetic divergence
between the six-row Spanish barleys and the reference cultivars, whereas this was not evident for the two-row barleys. A model-based
clustering analysis identified an underlying population structure, consisting of four main populations for the whole genotype
set, and suggested further possible subdivision within two of these populations. Most of the six-row Spanish landraces clustered
into two groups that corresponded to geographic regions with contrasting environmental conditions. The existence of wide genetic
diversity in Spanish germplasm, possibly related to adaptation to a broad range of environmental conditions, and its divergence
from current European cultivars confirm its potential as a new resource for barley breeders, and make the SBCC a valuable
tool for the study of adaptation in barley.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
2.
J. L. Molina-Cano P. Fra-Mon G. Salcedo C. Aragoncillo F. Roca de Togores F. García-Olmedo 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1987,73(4):531-536
Summary The distribution of genetic variants of a group of low molecular weight, chloroform-methanol soluble proteins (CM proteins), among Moroccan and non-Moroccan accessions of Hordeum spontaneum and among selections from several Moroccan landraces of H. vulgare and cultivars of the same species with widespread European origin, suggests that domestication of barley might have taken place in Morocco. An agromorphological characterization of the H. spontaneum accessions further supports this hypothesis. The possible Moroccan origin of the French cultivar Hatif de Grignon and of several Spanish 6-rowed barleys is also presented. 相似文献
3.
One hundred and six accessions of wild barley collected from Tibet, China, including 50 entries of the two-rowed wild barley Hordeum vulgare ssp. spontaneum (HS), 29 entries of the six-rowed wild barley Hordeum vulgare ssp. agriocrithon (HA), and 27 entries of the six-rowed wild barley Hordeum vulgare ssp. agriocrithon var. lagunculiforme (HL), were analyzed using 30 SSR markers selected from the seven barley linkage groups for studying genetic diversity and evolutionary relationship of the three subspecies of Tibetan wild barley to cultivated barley in China. Over the 30 genetic loci that were studied, 229 alleles were identified among the 106 accessions, of which 70 were common alleles. H. vulgare ssp. spontaneum possesses about thrice more private alleles (2.83 alleles/locus) than HS (0.93 alleles/locus), whereas almost no private alleles were detected in HL. The genetic diversity among-subspecies is much higher than that within-subspecies. Generally, the genetic diversity among the three subspecies is of the order HS > HL > HA. Phylogenetic analysis of the 106 accessions showed that all the accessions of HS and HA was clustered in their own groups, whereas the 27 accessions of HL were separated into two groups (14 entries with group HS and the rest with group HA). This indicated that HL was an intermediate form between HS and HA. Based on this study and previous works, we suggested that Chinese cultivated barley might evolve from HS via HL to HA. 相似文献
4.
Yadong Huang Benjamin P. Millett Karen A. Beaubien Stephanie K. Dahl Brian J. Steffenson Kevin P. Smith Gary J. Muehlbauer 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2013,126(3):619-636
Fusarium head blight (FHB) is a threat to barley (Hordeum vulgare L.) production in many parts of the world. A number of barley accessions with partial resistance have been reported and used in mapping experiments to identify quantitative trait loci (QTL) associated with FHB resistance. Here, we present a set of barley germplasm that exhibits FHB resistance identified through screening a global collection of 23,255 wild (Hordeum vulgare ssp. spontaneum) and cultivated (Hordeum vulgare ssp. vulgare) accessions. Seventy-eight accessions were classified as resistant or moderately resistant. The collection of FHB resistant accessions consists of 5, 27, 46 of winter, wild and spring barley, respectively. The population structure and genetic relationships of the germplasm were investigated with 1,727 Diversity Array Technology (DArT) markers. Multiple clustering analyses suggest the presence of four subpopulations. Within cultivated barley, substructure is largely centered on spike morphology and growth habit. Analysis of molecular variance indicated highly significant genetic variance among clusters and within clusters, suggesting that the FHB resistant sources have broad genetic diversity. The haplotype diversity was characterized with DArT markers associated with the four FHB QTLs on chromosome 2H bin8, 10 and 13 and 6H bin7. In general, the wild barley accessions had distinct haplotypes from those of cultivated barley. The haplotype of the resistant source Chevron was the most prevalent in all four QTL regions, followed by those of the resistant sources Fredrickson and CIho4196. These resistant QTL haplotypes were rare in the susceptible cultivars and accessions grown in the upper Midwest USA. Some two- and six-rowed accessions were identified with high FHB resistance, but contained distinct haplotypes at FHB QTLs from known resistance sources. These germplasm warrant further genetic studies and possible incorporation into barley breeding programs. 相似文献
5.
The Horn of Africa as a centre of barley diversification and a potential domestication site 总被引:2,自引:1,他引:1
Orabi J Backes G Wolday A Yahyaoui A Jahoor A 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2007,114(6):1117-1127
According to a widely accepted theory on barley domestication, wild barley (Hordeum vulgare ssp. spontaneum) from the Fertile Crescent is the progenitor of all cultivated barley (H. vulgare ssp. vulgare). To determine whether barley has undergone one or more domestication events, barley accessions from three continents have
been studied (a) using 38 nuclear SSR (nuSSRs) markers, (b) using five chloroplast SSR (cpSSR) markers yielding 5 polymorphic
loci and (c) by detecting the differences in a 468 bp fragment from the non-coding region of chloroplast DNA. A clear separation
was found between Eritrean/Ethiopian barley and barley from West Asia and North Africa (WANA) as well as from Europe. The
data from chloroplast DNA clearly indicate that the wild barley (H. vulgare ssp. spontaneum) as it is found today in the “Fertile Crescent” might not be the progenitor of the barley cultivated in Eritrea (and Ethiopia).
Consequently, an independent domestication might have taken place at the Horn of Africa.
Jihad Orabi and Gunter Backes have contributed equally to this work. 相似文献
6.
Tanno K Taketa S Takeda K Komatsuda T 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2002,104(1):54-60
The origin of six-rowed cultivated barley was studied using a DNA marker cMWG699 closely linked to the vrs1 locus. Restriction patterns of the PCR-amplified product of the cMWG699 locus were examined in 280 cultivated (Hordeum vulgare ssp. vulgare) and 183 wild (H. vulgare ssp. spontaneum) barleys. Nucleotide sequences of the PCR products were also examined in selected accessions. Six-rowed cultivated barleys
were divided into two distinct groups, types I and II. Type I six-rowed cultivated barley was distributed widely while type
II six-rowed cultivated barley was found only in the Mediterranean region. The type I sequence was also found in a wild barley
accession from Turkmenistan whereas the type II sequence was also found in a two-rowed cultivated barley from North Africa
and a wild barley from Morocco. These results suggested that the six-rowed type I and II barleys were derived from two-rowed
type I and II barleys, respectively, by independent mutations at the vrs1 locus.
Received: 3 November 2000 / Accepted: 17 April 2001 相似文献
7.
Distribution of MWG699 polymorphism in Spanish European barleys. 总被引:3,自引:0,他引:3
The STS marker MWG699/TaqI is closely linked to the vrs1 locus and has been proposed as a marker of domestication in barley. This study included 257 cultivated barleys of both two- and six-rowed varieties, mainly from the western Mediterranean region. These included many landraces from the Spanish barley core collection, Moroccan landraces, and a set of accessions from other European countries. Restriction analysis of amplified DNA revealed three alleles, as previously described. Most of the two-rowed entries had the same allele, type K. Six-rowed entries showed both types A and D. Indeed, type D was widespread among Spanish landraces and commercial varieties from central Europe. It was also found in some two-rowed landraces originating from Spain and Morocco. Barleys with the D haplotype were predominantly winter types, whereas the A haplotype was evenly distributed among spring and winter types. These results support the existence of two different genetic sources among six-rowed Spanish landraces. 相似文献
8.
L. Petersen H. Østergård H. Giese 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1994,89(6):676-681
Genetic variability of cultivated and wild barley, Hordeum vulgare ssp. vulgare and spontaneum, respectively, was assessed by RFLP analysis. The material consisted of 13 European varietes, single-plant offspring lines of eight land races from Ethiopia and Nepal, and five accessions of ssp. spontaneum from Israel, Iran and Turkey. Seventeen out of twenty-one studied cDNA and gDNA probes distributed across all seven barley chromosomes revealed polymorphism when DNA was digested with one of four restriction enzymes. A tree based on genetic distances using frequencies of RFLP banding patterns was estimated and the barley lines clustered into five groups reflecting geographical origin. The geographical groups of land-race lines showed less intragroup variation than the geographical groups of spontaneum lines. The group of European varieties, representing large variation in agronomic traits, showed an intermediate level. The proportion of gene diversity residing among geographical groups (FST) varied from 0.19 to 0.94 (average 0.54) per RFLP pattern, indicating large diversification between geographical groups. 相似文献
9.
A comparative analysis of genetic polymorphism in wild and cultivated barley from Tibet using isozyme and ribosomal DNA markers. 总被引:8,自引:0,他引:8
This study was conducted to address some of the issues concerning the possible significance of Tibet in the origin and evolution of cultivated barley. A total of 1757 barley accessions from Tibet, including 1496 entries of Hordeum vulgare ssp. vulgare (HV), 229 entries of the six-rowed wild barley H. vulgare ssp. agriocrithon (HA), and 32 entries of the two-rowed wild barley H. vulgare ssp. spontaneum (HS), were assayed for allozymes at four esterase loci. A subsample of 491 accessions was surveyed for spacer-length polymorphism at two ribosomal DNA loci. Genetic variation is extensive in these barley groups, and the amount of genetic diversity in cultivated barley of this region is comparable with that of cultivated barley worldwide. The level of genetic variation of HA is significantly lower than the other two barley groups, and there is also substantial heterogeneity in the level of polymorphism among different agrigeographical subregions. However, little genetic differentiation was detected among the three barley groups (HV, HA, and HS), as well as among different agrigeographical subregions. Comparison of the results from this and previous studies indicated a strong differentiation between Oriental and Occidental barley, thus favoring the hypothesis of a diphyletic origin of cultivated barley. 相似文献
10.
Background
Among the cereal crops, barley is the species with the greatest adaptability to a wide range of environments. To determine the level and structure of genetic diversity in barley (Hordeum vulgare L.) landraces from the central highlands of Ethiopia, we have examined the molecular variation at seven nuclear microsatellite loci. 相似文献11.
应用微卫星标记研究西藏野生大麦的遗传多样性 总被引:9,自引:0,他引:9
以西藏不同地区的106份野生大麦为材料,其中包括50份野生二棱大麦(HS),27份野生瓶形大麦(HL)和29份野生六棱大麦(HA),用Liu等(1996)发表的SSR连锁图的每个连锁群的两个臂的不同位置上选取3~5个共30个SSR标记,研究了西藏3类野生大麦的遗传多样性。结果表明,这3类野生大麦在遗传组成及等位变异频率分布上存在着明显的遗传分化。在总样本中,共检测到229个等位变异,平均每个SSR位点检测到7.6个等位变异,其中70个为这3类野生大麦间共同的等位变异,等位变异数在这3类野生大麦间有明显的差异,亚种问的遗传多样性明显高于亚种内的遗传多样性。其遗传多样性大小顺序为HS〉HL〉HA。聚类分析表明,野生二棱大麦、野生六棱大麦分别聚在不同的两类,而野生瓶形大麦中各有约50%的材料分别聚在这两类。根据本研究及前人研究结果,我们认为中国栽培大麦是从野生二棱大麦经野生瓶形大麦向野生六棱大麦进化的。该结果支持了栽培大麦起源的“野生二棱大麦单系起源论”的观点。 相似文献
12.
Comparison of restriction fragment length polymorphisms in wild and cultivated barley. 总被引:1,自引:0,他引:1
This study was undertaken to assess the relative level of molecular diversity between cultivated barley, Hordeum vulgare ssp. vulgare (HV), and one of its wild relatives, H. vulgare ssp. spontaneum (HS), and to identify possible restriction fragment length polymorphism (RFLP) patterns that may provide information concerning the phylogenetic relationship between these two barley groups. A total of 363 barley accessions were assayed, including 95 entries of HV collected from 36 major barley growing countries of the world and 268 entries of HS from 25 natural populations in Israel and Iran. The 26 RFLP marker loci used in the survey represent single-copy, low-copy, and repetitive DNA sequences and mark all of the chromosome arms. A randomization test, on the basis of equal sample sizes, showed that HS is more polymorphic than HV, as evaluated by the number of alleles and diversity indices. The analysis also indicated extensive RFLP differentiation between these two barley groups; highly significant differences of allele frequencies were detected at the majority of the loci. The HV sample can be subdivided according to winter or spring growth habits, and two- or six-rowed spikes. Analysis of genetic polymorphisms in these subgroups showed that levels of diversity were about equal in spring and winter groups and also in the groups with two- and six-rowed spikes. However, significant differences of allelic frequencies were detected between subgroups of the two divisions. 相似文献
13.
Local cultivars adapted to specific environmental conditions are the chief source of seed for farmers in Ethiopia and deserve
research priority. The aim of this study was, therefore, to determine the genetic relationships between different barley landraces,
from north Shewa in Ethiopia so as to differentiate genotypes known by different local names and facilitate their conservation
and use in breeding new varieties. Five AFLP primer combinations were analyzed for 19 barley landraces and five malting varieties.
The number of scoreable fragments amplified by each AFLP primer combination varied from 49 to 118 with an average of 84.5
and polymorphic fragments for each primer combination varied from 27 to 77 with an average of 58.5. The average percent polymorphism
was 69.9% with values ranging from 55.1% to 75.8%. Cluster analysis placed the accessions and malting varieties into one main
group while all the farmers’ cultivars, with the exception of two, were in the other main group. It was shown that sampling
of germplasm at a given locality might not represent the whole array of genetic variability of locally grown famers’ cultivars.
A comprehensive study of all the farmers’ barley cultivars, grown in different parts of Ethiopia, is required to maximize
the efforts of germplasm conservation and utilization in national and regional breeding programs. 相似文献
14.
Arnold J. Bloom 《Oecologia》1985,65(4):555-557
Summary The kinetics of net ammonium influx were very similar among several cultivars of barley (Hordeum vulgare) and several accessions of the wild taxa H.v. spontaneum and H. jubatum. For net nitrate influx, variation was greater among accessions than among species; accessions from warmer climates had faster rates than those from colder climates. These data indicate that domestication of barley has not reduced its affinity for mineral nitrogen. 相似文献
15.
Genetic diversity among East Asian accessions of the barley core collection as revealed by six isozyme loci 总被引:3,自引:0,他引:3
F. Liu R. von Bothmer B. Salomon 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,98(8):1226-1233
Studies of allelic variations at six isozyme loci revealed genetic diversity of 380 East Asian accessions of the Barley Core
Collection. Genetic variation was found in both cultivars and landraces in different regions. Allelic variations at the Aco-1 and Aco-2 loci were detected for East Asian barley for the first time. Moreover, the Aco-1 locus displayed the highest genetic diversity among the six loci assayed. Indian cultivars showed the highest diversity,
followed by Korean and Chinese cultivars. Landraces from Bhutan and Nepal showed the lowest diversity. Cultivars had generally
higher diversity than landraces within as well as among regions. The cluster analysis of genetic identity showed that all
landraces from different countries can be placed in one group; the cultivars from Japan, India and Korea each form independent
groups. Gpi-1 Gu, Pgd-1 Tj, Aco-1 Si, Ndh-2 D and Aco-2 A were rare alleles found in only a few accessions of 6-rowed barley. The Pgd-2 Tn allele was very rare in East Asian accessions.
Received: 29 July 1998 / Accepted: 2 November 1998 相似文献
16.
The least ambiguous genetic markers are those based on completely characterized DNA sequence polymorphisms. Unfortunately, assaying allele states by allele sequencing is slow and cumbersome. The most desirable type of genetic marker would be unambiguous, inexpensive to assay and would be assayable singly or in parallel with hundreds of other markers (multiplexable). In this report we sequenced alleles at 54 barley (Hordeum vulgare ssp. vulgare) loci, 38 of which contained single-nucleotide polymorphisms (SNPs). Many of these 38 loci contained multiple polymorphisms, and a total of 112 polymorphisms were scored in five barley genotypes. The polymorphism data set was analyzed both by using the individual mutations as cladistic characters and by reducing data for each locus to haplotypes. We compared the informativeness of these two approaches by consensus tree construction and bootstrap analysis. Both approaches provided similar results. Since some of the loci sequenced contained insertion/deletion events and multiple point mutations, we thought that these multiple-mutated loci might represent old alleles that predated the divergence of barley from H. spontaneum. We evaluated sequences from a sample of H. spontaneum accessions from the Eastern Mediterranean, and observed similar alleles present in both cultivated barley and H. spontaneum, suggesting either multiple domestication events or multiple transfers of genes between barley and its wild ancestor. 相似文献
17.
Zhang WS Li X Liu JB 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2007,114(6):1039-1050
The enzyme beta-amylase is one of the most important hydrolytic enzymes in the grain of malting barley and is encoded by the gene Bmy1. To learn more about its structure and function, a total of 657 barley accessions including 541 Hordeum vulgare ssp. vulgare (HV), and 116 H. vulgare ssp. spontaneum (HS) were selected for the cleaved amplified polymorphic sequence (CAPS) analysis. These materials, covering all the 16 kinds of beta-amylase phenotypes screened from more than 8,500 accessions of the world barley germplasm, were classified into 13 CAPS types in the present study. A combined assay of phenotypes and CAPS types revealed extensive genetic variation at the Bmy1 locus, and in total 23 Bmy1 allele types were identified. The newly identified alleles (A-I-11, A-II-6, A-II-7, A-II-10, B-I-3, B-I-12 and B-I-13) provided us with a novel resource for barley breeding and Bmy1 study. In HV barley, six out of seven major allele types (C-II-1, B-II-2, B-Ia-3, A-II-5, A-II-6, and A-II-7) were shared with HS barley; the B-I-8 allele, which was predominant in north European cultivated barley, was found to be unique. Remarkably, very low Bmy1 genetic variation was detected in Tibetan barleys, which puts the validity of the hypothesis that Tibet is one of the original centers of cultivated barley into question. 相似文献
18.
Molecular mapping of a new gene in wild barley conferring complete resistance to leaf rust (Puccinia hordei Otth) 总被引:2,自引:0,他引:2
V. Ivandic U. Walther A. Graner 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,97(8):1235-1239
A dominant gene conferring resistance to all known races of Puccinia hordei Otth was identified in two accessions of Hordeum vulgare ssp. spontaneum. Using restriction fragment length polymorphism (RFLP) markers the gene was mapped on chromosome 2HS in doubled-haploid populations
derived from crosses of both accessions to the susceptible cultivar L94. Until now, complete leaf rust resistance was not
known to be conditioned by genetic factors on this barley chromosome. Therefore, the designation Rph16 is proposed for the gene described in this study. A series of sequence tagged site (STS) and cleaved amplified polymorphic
sequence (CAPS) markers were generated by conversion of RFLP probes which originate from the chromosomal region carrying the
resistance gene. Two PCR-based markers were shown to co-segregate with the Rph16 gene in both populations thus providing the basis for marker-assisted selection.
Received: 20 May 1998 / Accepted: 9 June 1998 相似文献
19.
Jones H Leigh FJ Mackay I Bower MA Smith LM Charles MP Jones G Jones MK Brown TA Powell W 《Molecular biology and evolution》2008,25(10):2211-2219
Gene resequencing and association analysis present new opportunities to study the evolution of adaptive traits in crop plants. Here we apply these tools to an extensive set of barley accessions to identify a component of the molecular basis of the flowering time adaptation, a trait critical to plant survival. Using an association-based study to relate variation in flowering time to sequence-based polymorphisms in the Ppd-H1 gene, we identify a causative polymorphism (SNP48) that accounts for the observed variation in barley flowering time. This polymorphism also shows latitude-dependent geographical distribution, consistent with the expected clinal variation in phenotype with the nonresponsive form predominating in the north. Networks, genealogies, and phylogenetic trees drawn for the Ppd-H1 haplotypes reveal population structure both in wild barley and in domesticated barley landraces. The spatial distribution of these population groups indicates that phylogeographical analysis of European landraces can provide information relevant to the Neolithic spread of barley cultivation and also has implications for the origins of domesticated barley, including those with the nonresponsive ppd-H1 phenotype. Haplotypes containing the nonresponsive version of SNP48 are present in wild barley accessions, indicating that the nonresponsive phenotype of European landraces originated in wild barley. The wild accessions whose nonresponsive haplotypes are most closely similar to those of landraces are found in Iran, within a region suggested as an area for domestication of barley east of the Fertile Crescent but which has previously been thought to have contributed relatively little to the diversity of European cultivars. 相似文献
20.
Phenotypic variation in barley (Hordeum vulgare L.) landraces from north Shewa in Ethiopia 总被引:1,自引:0,他引:1
An experiment was conducted in a greenhouse to estimate the level of morphological variability within 44 barley landraces (14 farmers' cultivars and 30 accessions) from north Shewa in Ethiopia. Four qualitative traits (spike type, kernel color, caryopsis type and spike density) were recorded. Variation in spike type (two-rowed, irregular or six-rowed) was high in many landraces. Variability in spike type within landraces from Debre Libanose, Kuyu and Girar Jarso was high. The highest mean frequencies of the six-row type were recorded for Ankober-Mezezo (85%) and Kimbibit (80.9%). The two-row types were dominant in Wuchale (53.3%), while they were absent at the Ankober-Mezezo, Debre Libanose and Kimbibit localities. A larger proportion of the irregular types occurred in Debre Libanose (71.3%), followed by Kuyu (62.6%). Diversity for kernel color was generally low except in landraces from Kuyu, but it was very high among landraces. White kernel color was predominant. The mean diversity index pooled over characters ranged from 0.12 ± 0.08 to 0.57 ± 0.11, and 11 landraces had a mean diversity larger than 0.50. Differences among landraces within localities were mostly highly significant for all characters except that of caryopsis type (covered or naked). Landraces from Girar Jarso, Wuchale, and Kuyu in particular differed significantly in spike type and kernel color. Unlike farmers' cultivars, the accessions were found to be more variable in spike type and kernel color. Moreover, the mean diversity indices pooled over characters were relatively higher in the accessions than within farmers' cultivars. 相似文献