共查询到20条相似文献,搜索用时 15 毫秒
1.
Investigations on the effects of low levels of Al on P adsorption,uptake and translocation in seedlings of the indigenous grassAgrostis capillaris were undertaken. Apparent uptake and transportof H2 32PO4 from nutrient solutions containing 10 or 100mmolm3 phosphate were characterized as functions of timeand concentration. Experiments on 32P uptake and transport insolutions containing no Al (control) or Al ranging from 3.7to 185 mmol m3 at pH ranging from 4.3 to 4.6, showedthat in 10 mmol m3 P, effects of Al at 3.7 and 37 mmolm3 on the size of the initial uptake shoulder were small,but some increase in subsequent P uptake to the roots was observed,though transport to the shoots was suppressed. With 37 mmolm3 Al in nutrient solution containing 100 mmol m3P, the uptake shoulder was much increased above the control.Subsequent root uptake was stimulated but transport was unaffected.Lack of toxicity of the Al concentrations used was indicatedby a lack of significant effect on plant fresh weight. AbsorbedAl was almost totally retained in the root in all treatments.Speciation calculations showed that the major species in Alamended nutrient solution at pH 4.4 were H2PO4, AI3+and AIHPO4+, together with substantial amounts of AISO4+ andsoluble aluminium hydroxy complexes (AIOH2+, AI(OH)2+), dependingon the relative concentrations of P and Al. The effects of Al,with 10 mmol m3 P, on adsorption of complexed P werepartly accounted for in terms of preferential cell wall adsorptionof Al complexes not containing P. Conclusions were drawn aboutthe P-economy of A. capillaris plants growing on soils withlow levels of P and Al. Key words: Phosphorus, aluminium, speciation, Agrostis capillahs L 相似文献
2.
Longest root elongation diminished significantly in the three species tested from 6 mm d-1 to 3 mm d-1 in 3 weeks. During this period S.D. increased considerably (from 49% to 112%, A. castellana), and accounted on the average for 68% (A. capillaris) till 94% (A. castellana) of the mean. Maximum root growth stabilized at 6 mm d-1 and showed less variation in the measurements (S.D. 52% of the mean). Growth of the originally longest root approaches zero in all three species, in accordance with the natural cease of growth of roots in grasses fascicular root system. Measuring maximum root growth instead of longest root elongation is proposed for testing metal tolerance of grasses in sequential experiments. 相似文献
3.
We experimentally studied the role of local adaptation and the co-evolutionary relationship between an annual, endangered root hemiparasite Euphrasia rostkoviana and its main host Agrostis capillaris. According to our hypothesis, the existence of local adaptation in hemiparasites should be observable in better hemiparasite performance when attached to A. capillaris hosts originating From Euphrasia populations. After one month of growth, the height and the number of leaves of hemiparasites were not affected by the origin of their hosts. The differences in growth were due to between population effects. The situation remained constant after three months. Hemiparasite biomass was not affected by the origin of the hosts. The percentage of hemiparasites surviving after one. two and three months was not affected by the origin of the hosts although there was a weak tendency towards better survival of hemiparasites with familiar hosts than with unfamiliar hosts. All variables used to measure hemiparasite performance during its complete life-cycle gave only limited support for the local adaptation hypothesis. Nevertheless, the familiar hosts suffered less from parasitism as indicated by their higher biomass after the experiment. This suggests that there may be some interactions between hemiparasites and their hosts based on their spatial population structure and common history as competitors. 相似文献
4.
Fluxes of a nonelectrolyte and compartmentation in cells of carrot root tissue 总被引:2,自引:2,他引:0
下载免费PDF全文
![点击此处可从《Plant physiology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Glinka Z 《Plant physiology》1974,53(2):307-311
Permeation of thiourea into cells of carrot (Daucus carota L.) storage root discs was found to be governed by its concentration gradient only. The analysis of the course of thiourea efflux from preloaded discs shows two distinct exponential curves, apart from an initial very fast component. Reasons are given for relating the first exponential part of the curve to efflux from the cytoplasm and the second exponential part to efflux from the vacuole. 相似文献
5.
The double fluorescence staining with acridine orange and ethidium bromide (AO/EB) revealed that treatment of Vicia faba ssp. minor seedlings with kinetin-induced programmed cell death (PCD) in root cortex cells. Kinetin-induced cell death reflected by the morphological changes of nuclei including their invagination, volume increase, chromatin condensation and degradation as well as formation of micronuclei showed by AO/EB and 4,6-diamidino-2-phenylindol staining was accompanied by changes including increase in conductivity of cell electrolytes secreted to culture media, decrease in the number of the G1- and G2-phase cells and appearance of fraction of hypoploid cells as the effect of DNA degradation without ladder formation. Decrease in the number of mitochondria and in the activity of cellular dehydrogenases, production of reactive oxygen species (ROS), appearance of small and then large lytic vacuoles and increase in the amount of cytosolic calcium ions were also observed. The PCD was also manifested by increased width and weight of apical fragments of roots as well as decreased length of cortex cells which led to shortening of the whole roots. The kinetin-induced PCD process was almost completely inhibited by adenine, an inhibitor of phosphoribosyl transferase, and mannitol, an inhibitor of ROS production. These cell-death hallmarks and pathway of this process suggested that the induction of kinetin-specific vacuolar type of death, expressed itself with similar intensity on both morphological and metabolic levels, was a transient protecting whole roots and whole seedlings against elimination. 相似文献
6.
7.
Noninvasive and continuous recordings of auxin fluxes in intact root apex with a carbon nanotube-modified and self-referencing microelectrode 总被引:1,自引:0,他引:1
Auxin (also known as indole-3-acetic acid, IAA) represents an ancient signaling molecule of plants that also exerts bioactive actions on yeast and animal cells. Importantly, IAA emerges as a new anticancer agent due to the ability of oxidatively activated IAA to selectively kill tumor cells. IAA acts as a pheromone-like molecule in brown algae, whereas the hormone concept of IAA dominates current plant biology. However, recent advances also favor the morphogen- and transmitter-like nature of IAA in plants, making this small molecule one of the most unique molecules in the eukaryotic superkingdom. Here, we introduce new technology for the continuous measuring of IAA fluxes in living cells, tissues, and whole organs that is based on a carbon nanotube-modified and self-referencing microelectrode specific for IAA. This technique not only will advance our knowledge of how IAA regulates plant development but will also be applicable in medicine for its potential use in cancer therapy. 相似文献
8.
Peter M. Kopittke Enzo Lombi Brigid A. McKenna Peng Wang Erica Donner Richard I. Webb F. Pax C. Blamey Martin D. de Jonge David Paterson Daryl L. Howard Neal W. Menzies 《Physiologia plantarum》2013,147(4):453-464
The phytotoxicity of Mn is important globally due to its increased solubility in acid or waterlogged soils. Short‐term (≤24 h) solution culture studies with 150 µM Mn were conducted to investigate the in situ distribution and speciation of Mn in apical tissues of hydrated roots of cowpea [Vigna unguiculata (L.) Walp. cv. Red Caloona] using synchrotron‐based techniques. Accumulation of Mn was rapid; exposure to 150 µM Mn for only 5 min resulting in substantial Mn accumulation in the root cap and associated mucigel. The highest tissue concentrations of Mn were in the root cap, with linear combination fitting of the data suggesting that ≥80% of this Mn(II) was associated with citrate. Interestingly, although the primary site of Mn toxicity is typically the shoots, concentrations of Mn in the stele of the root were not noticeably higher than in the surrounding cortical tissues in the short‐term (≤24 h). The data provided here from the in situ analyses of hydrated roots exposed to excess Mn are, to our knowledge, the first of this type to be reported for Mn and provide important information regarding plant responses to high Mn in the rooting environment. 相似文献
9.
Zinc compartmentation in root, transport into xylem, and absorption into leaf cells in the hyperaccumulating species of Sedum alfredii Hance 总被引:5,自引:0,他引:5
Sedum alfredii Hance can accumulate Zn in shoots over 2%. Leaf and stem Zn concentrations of the hyperaccumulating ecotype (HE) were 24- and 28-fold higher, respectively, than those of the nonhyperaccumulating ecotype (NHE), whereas 1.4-fold more Zn was accumulated in the roots of the NHE. Approximately 2.7-fold more Zn was stored in the root vacuoles of the NHE, and thus became unavailable for loading into the xylem and subsequent translocation to shoot. Long-term efflux of absorbed 65Zn indicated that 65Zn activity was 6.8-fold higher in shoots but 3.7-fold lower in roots of the HE. At lower Zn levels (10 and 100 μM), there were no significant differences in 65Zn uptake by leaf sections and intact leaf protoplasts between the two ecotypes except that 1.5-fold more 65Zn was accumulated in leaf sections of the HE than in those of the NHE after exposure to 100 μM for 48 h. At 1,000 μM Zn, however, approximately 2.1-fold more Zn was taken up by the HE leaf sections and 1.5-fold more 65Zn taken up by the HE protoplasts as compared to the NHE at exposure times >16 h and >10 min, respectively. Treatments with carbonyl cyanide m-chlorophenylhydrazone (CCCP) or ruptured protoplasts strongly inhibited 65Zn uptake into leaf protoplasts for both ecotypes. Citric acid and Val concentrations in leaves and stems significantly increased for the HE, but decreased or had minimal changes for the NHE in response to raised Zn levels. These results indicate that altered Zn transport across tonoplast in the root and stimulated Zn uptake in the leaf cells are the major mechanisms involved in the strong Zn hyperaccumulation observed in S. alfredii H. 相似文献
10.
11.
12.
The potential role of diamine oxidase (DAO) and polyamine oxidase (PAO) in relation to polyamines was investigated in epicotyls,
roots and leaf blades at 3 and 6 days after gibberellic acid (GA) application in barley (Hordeum vulgare L.) seedlings of cvs. Maythorpe (non-mutant parent) and Golden Promise (semi-dwarf mutant). There was a significant increase
in epicotyl and leaf-blade elongation rates in GA-treated seedlings of cv. Maythorpe as compared to cv. Golden Promise. DAO
and PAO were detectable in all segments of the leaf blade, but the highest activities were present in basal segments. These
enzymes, which are thought to have a role in the elimination of cellular polyamines, increased in activity following GA application
compared to controls. Application of 10−6 M GA to the first leaf, significantly increased endogenous bound putrescine (Put) levels in both the epicotyl and leaf blade
of cv. Maythorpe. In contrast, there was only a slight increase in cv. Golden Promise. Levels of soluble Put increased in
roots and leaf blades of both cultivars following GA treatment but the effect was greatest in leaves of cv. Maythorpe. It
is suggested that polyamines may play a role in GA-induced epicotyl and leaf-blade elongation in barley. 相似文献
13.
14.
The resistance of some plants to Al (aluminium or aluminum) has been attributed to the secretion of Al(3+)-binding organic acid (OA) anions from the Al-sensitive root tips. Evidence for the 'OA secretion hypothesis' of resistance is substantial, but the mode of action remains unknown because the OA secretion appears to be too small to reduce adequately the activity of Al(3+) at the root surface. In this study a mechanism for the reduction of Al(3+) at the root surface and just beneath the epidermis by complexation with secreted OA(2-) is considered. According to our computations, Al(3+) activity is insufficiently reduced at the surface of the root tips to account for the Al resistance of Triticum aestivum L. cv. Atlas 66, a malate-secreting wheat. Experimental treatments to decrease the thickness of the unstirred layer (increased aeration and removal of root-tip mucilage) failed to enhance sensitivity to Al(3+). On the basis of additional modelling, the observed spatial distribution of Al in roots, and the anatomical responses to Al, it is proposed that the epidermis is an essential component of the diffusion pathway for both OA and Al. We suggest that Al(3+) in the cortex must be reduced to small concentrations in order substantially to alleviate the inhibition of root elongation and so that the outer surface of the epidermis can tolerate relatively large concentrations of Al(3+). If OA secretion is required for reducing Al(3+) mainly beneath the root surface, rather than in the rhizosphere, then the metabolic cost to plants will be greatly reduced. 相似文献
15.
Response and tolerance of root border cells to aluminum toxicity in soybean seedlings 总被引:2,自引:0,他引:2
Root border cells (RBCs) and their secreted mucilage are suggested to participate in the resistance against toxic metal cations, including aluminum (Al), in the rhizosphere. However, the mechanisms by which the individual cell populations respond to Al and their role in Al resistance still remain unclear. In this research, the response and tolerance of RBCs to Al toxicity were investigated in the root tips of two soybean cultivars [Zhechun No. 2 (Al-tolerant cultivar) and Huachun No. 18 (Al-sensitive cultivar)]. Al inhibited root elongation and increased pectin methylesterase (PME) activity in the root tip. Removal of RBCs from the root tips resulted in a more severe inhibition of root elongation, especially in Huachun No. 18. Increasing Al levels and treatment time decreased the relative percent viability of RBCs in situ and in vitro in both soybean cultivars. Al application significantly increased mucilage layer thickness around the detached RBCs of both cultivars. Additionally, a significantly higher relative percent cell viability of attached and detached RBCs and thicker mucilage layers were observed in Zhechun No. 2. The higher viability of attached and detached RBCs, as well as the thickening of the mucilage layer in separated RBCs, suggest that RBCs play an important role in protecting root apices from Al toxicity. 相似文献
16.
Panda SK 《Journal of plant physiology》2007,164(11):1419-1428
Oxidative stress and ultrastructural changes under hexavalent chromium stress were investigated in developing rice seedlings. Chromium treatment for 24 or 48h resulted in inhibition of root length and dry biomass. Atomic absorption spectrometry analysis of roots showed that chromium accumulation increased with increase in concentration and duration of metal treatment. Chromium resulted in increased production of hydrogen peroxide and superoxide radical in root cells, which was a significant change after 48h of treatment. Time-course analysis of malondialdehyde content showed no substantial variation during early treatment periods (2, 6 or 12h). Increase in malondialdehyde content was observed only after 18h and it continued to increase until 48h after treatment. Loss of membrane integrity, analyzed in terms of Evans blue uptake in root cells, showed an increase in uptake of the reagent, indicating loss of membrane integrity. The antioxidant enzyme, viz., guaiacol peroxidase, was least affected, while glutathione reductase showed significant decline after 24 or 48h of metal treatment, followed by increased activity of superoxide dismutase. The level of ascorbate was not affected by chromium, while an increase in the level of glutathione was observed. At the ultrastructural level, potential damage to the root cell was noted after 48h at 100microM of chromium compared with controls. 相似文献
17.
Trypanosoma brucei: fluxes of the morphological variants in intact and X-irradiated mice 总被引:10,自引:0,他引:10
A E Balber 《Experimental parasitology》1972,31(2):307-319
The number of dividing, slender, intermediate, and stumpy forms of Trypanosoma brucei in the blood of inbred mice changed daily. In both intact mice and mice which were exposed to whole body X-irradiation before infection, slender and dividing forms predominated during the first 72 hr of infection, and the number of intermediate and stumpy forms increased to a maximum between 72 and 140 hr. The rate at which stumpy forms accumulated in the blood and the number of these forms which eventually circulated were the same in both groups. However, the fluxes in the number of slender and dividing forms differed in intact and X-irradiated mice. In intact mice, the number of dividing forms in the blood decreased between 72 and 140 hr, and the number of slender forms decreased between 96 and 140 hr. In X-irradiated mice, the number of both these forms increased throughout infection. Electrophoresis of serum proteins and agglutination tests showed that X-irradiation severely depressed the ability of the mice to make antibody. Homogenates of spleen and bone marrow of intact mice contained many dividing forms throughout the infection. It is concluded that although host antibody does not directly induce the transformation of slender forms into stumpy forms, it may influence the morphological composition of the peripheral blood population of trypanosomes in several ways. 相似文献
18.
The effects of invasion by the cereal cyst nematode, Heterodera avenae, on the root growth of susceptible and resistant oat seedlings were similar. Nematode invasion directly inhibited root extension and lateral root production, which was dependent upon the developmental status of the invaded root tissue and the nematode density cm-1 root. Both responses are caused by mechanical damage due to nematode invasion leading to decreased top growth in the host. Reduced root development, decreased efficiency of water and nutrient uptake in invaded plants being indirect effects of nematode invasion. 相似文献
19.
D. A. Baker 《Planta》1973,112(4):293-299
Summary The accumulation of 86Rb labelled potassium by isolated stelar and cortical tissues from 7-day-old roots of Zea mays has been compared with the levels accumulated by these tissues in the intact root. Cortical tissues have similar uptake eapacities in these two conditions whereas stelar tissues only exhibit an uptake capacity in the intact root system. The uncoupler carbonylcyanide m-chlorophenylhydrazone caused a considerable decrease in the uptake of potassium by these tissues. In the intact root system it prevented ions from the bathing medium reaching the stelar tissues. The efflux pattern from preloaded isolated stelar and cortical tissues was considerably altered by the inhibitor, a promotion of the efflux occurring in both of these tissues.It is concluded that stelar tissues only accumulated ions when these are supplied through the root symplasm and that the stelar plasmalemma has only a limited uptake capacity per se. Stelar uptake is thus a reflection of vacuolar accumulation across the tonoplast. There is no evidence in the present study of a carrier-mediated active secretion of ions across the stelar plasmalemma. The fact that the efflux was promoted rather than depressed by the uncoupler supports the postulate that a passive leakage is the final stage in the transport of ions across the plant root. 相似文献
20.
N-Acylethanolamines (NAEs) are prevalent in desiccated seeds of various plant species, and their levels decline substantially during seed imbibition and germination. Here, seeds of Arabidopsis thaliana (L.) Heynh. were germinated in, and seedlings maintained on, micromolar concentrations of N-lauroylethanolamine (NAE 12:0). NAE 12:0 inhibited root elongation, increased radial swelling of root tips, and reduced root hair numbers in a highly selective and concentration-dependent manner. These effects were reversible when seedlings were transferred to NAE-free medium. Older seedlings (14 days old) acclimated to exogenous NAE by increased formation of lateral roots, and generally, these lateral roots did not exhibit the severe symptoms observed in primary roots. Cells of NAE-treated primary roots were swollen and irregular in shape, and in many cases showed evidence, at the light- and electron-microscope levels, of improper cell wall formation. Microtubule arrangement was disrupted in severely distorted cells close to the root tip, and endoplasmic reticulum (ER)-localized green fluorescent protein (mGFP5-ER) was more abundant, aggregated and distributed differently in NAE-treated root cells, suggesting disruption of proper cell division, endomembrane organization and vesicle trafficking. These results suggest that NAE 12:0 likely influences normal cell expansion in roots by interfering with intracellular membrane trafficking to and/or from the cell surface. The rapid metabolism of NAEs during seed imbibition/germination may be a mechanism to remove this endogenous class of lipid mediators to allow for synchronized membrane reorganization associated with cell expansion. 相似文献