共查询到20条相似文献,搜索用时 62 毫秒
1.
The effects of photoperiod (8, 12 or 16 h), mineral medium strength (dilutions of a tuberization medium, the T medium), sucrose
(0, 2, 4, 8% w/v) and kinetin (0, 2.5μM) on the development of roots, shoots and microtubers in shoot cultures of Dioscorea alata L. and D. bulbifera L. yams were evaluated. All of the factors were found to have substantial effects on microtuber induction in these two species.
The effects of high and low inorganic ammonium containing media on microtuberization of yam shoot cultures indicated that
ammonium ions inhibited microtuber induction in D. alata but not in D. bulbifera. Microtubers of D. alata were only formed on shoot cultures if these were held under 8-h days. D. bulbifera cultures on the other hand produced microtubers under this photoperiod treatment as well as under 16-h photoperiods provided
that kinetin was present in media at 2.5μM. Most microtuberization in D. alata shoot cultures occurred on full-strength T medium supplemented with 2% sucrose, 2.5μM kinetin held under 8-h photoperiod
at 25°C, whereas most microtuberization in D. bulbifera shoot cultures occurred on full-strength MS medium supplemented with 4% sucrose, 2.5μM kinetin held under 8-h photoperiods
at 25°C. Under these two sets of conditions, yam shoot cultures consistently produced microtubers with individual weights
in excess of 100 mg which were large enough to be capable of direct planting and subsequent growth in unsterilized soils. 相似文献
2.
Mészáros Annamária Bellon Andrea Pintér Éva Horváth Gábor 《Plant Cell, Tissue and Organ Culture》1999,57(2):149-152
Traditional propagation of lemon balm (Melissa officinalis L.) is inefficient for establishing a good quality clonal population.
Results of the presented experiments outline an effective method for micropropagation of this species. Following culture initiation
from shoots of field-grown plants on growth regulator free Murashige–Skoog medium, rapid shoot multiplication with only rudimentary
root formation could be achieved on media containing various concentrations of indole-3-acetic acid and 6-benzyladenine. The
combination of 5.71 μM indole-3-acetic acid and 6.66 μM 6-benzyladenine resulted in the best multiplication. Transfer of propagules
to media containing indole-3-acetic acid and kinetin did not result in shoot proliferation; however, single plantlets grown
on media containing 5.71 μM indole-3-acetic acid and 13.9 μM kinetin developed more compact shoots and stronger roots than
the control plants and were suitable for acclimatisation with an efficiency over 95%.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
3.
Federico A. Gutiérrez-Miceli Lourdes Arias Nicolás Juarez-Rodríguez Miguel Abud-Archila Aldo Amaro-Reyes Luc Dendooven 《In vitro cellular & developmental biology. Plant》2010,46(1):57-63
This paper reports on the optimum concentrations of naphthalene acetic acid (NAA) and 6-benzyladenine (BA) to stimulate callus
growth and NAA; kinetin and silver nitrate (AgNO3) for callus redifferentiation in Dianthus caryophyllus L. Meristems were excised and placed in MS medium with 30 g l−1 sucrose and 9.0 μM 2,4-d. Callus clusters were transferred to MS medium containing NAA (0, 1.7, 3.3, and 5.0 μM) and BA (0, 1.7, 3.3, and 5.0 μM)
for proliferation and to MS medium with 30 g l−1 sucrose, 2.5 g l−1 phytagel, kinetin (0, 33, and 66 μM); NAA (0, 7.95, and 15.9 μM) and AgNO3 (0, 23.54 and 47.08 μM) for shoot and root induction. Treatments were applied according to a Box–Behnken design. After callus
growth and redifferentiation, plants were incubated in the greenhouse at 18 ± 2°C for 4 wk and at 20–26°C for 4 wk. Finally,
plants were changed to near-commercial greenhouse conditions with different day (30–35°C) and night (16–24°C) temperatures.
Results showed better callus growth at higher NAA concentrations. A maximum callus weight was found with 5.0 μM NAA but without
BA. A maximum of 78% calluses with shoots was obtained with 15.9 μM NAA, 47.08 μM AgNO3, and 0.74 μM kinetin and 58% with roots with 15.7 μM NAA and 47.08 μM AgNO3, but without kinetin. The shoots obtained showed little hyperhydricity. Vigorous plants were obtained after gradual acclimatization
with an 80% survival rate under nursery conditions. 相似文献
4.
Organogenic cultures were induced from zygotic embryo and megagametophyte explants of the Central American cycad species,
Dioon edule. Plant growth medium consisted of B5 major salts, Murashige and Skoog minor salts and organics, 400 mg l−1 glutamine, 100 mg l−1 arginine, 100 mg l−1 asparagine, 60 g l−1 sucrose, 8 g l−1 Difco Bacto agar and was supplemented with kinetin (0 – 13.94 μM) and 2,4-dichlorophenoxyacetic acid (2,4-D) (0 – 9.05 μM)
arranged as a 5×4 factorial in a randomized block design. Callus initiation occurred on a wide range of medium formulations
from megagametophyte explants; however, shoot formation occurred only on medium supplemented with 2.26 μM 2,4-D. In comparison,
callus initiation from explanted zygotic embryos occurred on fewer medium formulations, and adventitious shoot induction occurred
from callus on formulations with 9.29–13.94 μM kinetin + 0.45–9.05 μM 2,4-D. Rooted shoots, derived from megagametophyte and
zygotic embryo cultures, have been regenerated.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
5.
Guohua Ma Jaime A. Teixeira da Silva Jinfeng Lü Xinhua Zhang Jietang Zhao 《Plant Cell, Tissue and Organ Culture》2011,105(3):355-361
An efficient propagation and regeneration system via direct shoot organogenesis for an endangered species, Metabriggsia ovalifolia, was established. High activity cytokinins [6-benzyladeneine (BA) and thidiazuron (TDZ)] and low activity auxins [α-naphthaleneacetic
acid (NAA), indole-3-butyric acid (IBA) and indole-3-acetic acid (IAA)] could directly induce adventitious shoots from leaf
or petiole explants within 5 weeks. Cytokinins (TDZ or BA) combined with auxin (NAA) in the induction media induced more adventitious
shoots than when auxins or cytokinins were used alone. Adventitious shoots could be induced and also mass-propagated on media
containing 2.5–5.0 μM TDZ (or BA) and 0.25–0.5 μM NAA. Adventitious roots differentiated at the proximal end of shoots on
rooting media containing half-strength MS salts and 0.5 μM IBA, 0.5 μM NAA, 0.1% activated charcoal or no plant growth regulators.
Over 90% of plantlets survived following acclimatization and transfer to a potting mixture (1:1, sand:vermiculite) in basins. 相似文献
6.
K. Chengalrayan M. Gallo-Meagher 《In vitro cellular & developmental biology. Plant》2001,37(4):434-439
Summary Sugarcane (Saccharum spp. hybrid cv. CP 84-1198) embryogenic calluses were induced from young leaves cultured on modified Murashige and Skoog
basal medium supplemented with 13.6 μM 2,4-dichlorophenoxyacetic acid. Five concentrations, 0.5, 1.0, 2.5, 5.0, and 10.0 μM, of five different growth regulators, 6-benzylaminopurine, kinetin, 6-γ,γ-(dimethylallylamino)purine, zeatin, and thidiazuron,
were tested with or without 22.5 μM α-naphthaleneacetic acid to compare their ability to induce regeneration from embryogenic callus. After 4 wk on medium, the
percentage of shoot meristem induction was evaluated, and after 10 wk the total number of shoots produced, as well as the
percentage of shoots greater than 1 cm in length, was obtained. Although it had the lowest percentage of elongated shoots,
medium containing thidiazuron alone performed better than all other growth regulators tested, with the highest percentage
of shoot induction and the largest number of shoots, particularly at a concentration of 2.5 μM. 相似文献
7.
An efficient method of micropropagation based on an increased percentage survival of explants and reduced phenol-induced browning
in wild strawberry has been developed. Serial transfer of nodal explants was carried out at 24-, 48- and 96-h intervals. Nodal
segments cultured on Murashige and Skoog medium supplemented with 6-benzyladenine (4.0 μM) and α-naphthalene acetic acid (0.1
μM) gave the best (94.4%) explant establishment and shoot number (22.3) per explant. Of the cytokinins tested, 6-benzyladenine
was found more effective than kinetin and N6-(γ,γ dimethylallyamino) purine. Excised shoots rooted on half-strength agar-gelled medium with 1.0 μM α-naphthalene acetic
acid. Rooted shoots with fully expanded leaves acclimatized successfully and about 70% of plantlets survived ex vitro.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
8.
Yong-Chang Qian Tuan Nguyen Terence M. Murphy 《Plant Cell, Tissue and Organ Culture》1993,34(3):245-252
The effects of plant growth regulators, light intensity, and end-of-day (EOD) light quality treatments on node and microtuber induction (% of cultures with microtubers) and development (fresh weight of microtubers) in yam (Dioscorea alata L. cv. Oriental) cultures were investigated. Nodal segments were excised from plantlets cultured on tuberization medium containing growth regulators and exposed to various light treatments. Absciscic acid (1 M) stimulated and cytokinins (2.5 M) inhibited microtuber development from yam nodal segments cultured on Mantell's and Hugo's full-strength tuberization medium under 8-h photoperiods. EOD far-red (FR) light inhibited microtuber induction and development and enhanced node formation. EOD FR light effects were nullified by immediately following the FR treatment with red light. This suggested the involvement of phytochrome in these processes. The lowest light intensity evaluated (12 mol m–2 s–1) inhibited microtuber, root and shoot production as compared to light intensities of 42, 72 and 102 mol m–2 s–1. Kinetin (2.5 m) in half-strength tuberization medium inhibited microtuber induction and development but did not affect node production in the light intensity evaluation.Abbreviations ABA
abscisic acid
- BA
6-benzylaminopurine
- 2iP
6-(c,c-dimethylallylamino)-purine
- NAA
napthaleneacetic acid
- R light
red light
- FR light
far-red light
- EOD light
end-of-day light 相似文献
9.
Guohua Ma Jinfeng Lü Jaime A. Teixeira da Silva Xinhua Zhang Jietang Zhao 《Plant Cell, Tissue and Organ Culture》2011,104(2):157-162
Ochna integerrima is a medicinal and ornamental plant in Southeastern Asia. It has been listed as a rare and endangered species in China. Here
we studied the effects of plant growth regulators and their concentrations on the induction of somatic embryogenesis and shoot
organogenesis from leaf and shoot explants of O. integerrima for the first time. Cytokinins played a crucial role in somatic embryogenesis and shoot organogenesis. Among them, a higher
concentration of thidiazuron (10.0–15.0 μM TDZ) could induce both somatic embryogenesis and adventitious shoot formation whereas
low concentrations of TDZ (5.0 μM) could only induce adventitious shoots. However, 6-benzyladenine (BA at 5–15 μM) could only
induce adventitious shoots. Shoot explants induced more adventitious shoots and somatic embryos than leaf explants when cultured
on medium with the same concentration (5–15 μM) of TDZ or 15 μM BA. Medium containing 0.5 μM α-naphthaleneacetic acid and
8 μM indole-3-butyric acid and 0.1% activated charcoal could induce adventitious roots within 1 month. An efficient mass propagation
and regeneration system has been established. 相似文献
10.
U. A. Jo H. N. Murthy E. J. Hahn K. Y. Paek 《In vitro cellular & developmental biology. Plant》2008,44(1):26-32
An efficient, simple micropropagation method was developed for Alocasia amazonica using corms in semisolid and liquid cultures. Explants were cultured onto Murashige and Skoog (MS) medium (Murashige and
Skoog, Physiol. Plant. 15:473–497, 1962) supplemented with different cytokinins (Benzyladenine [BA, 2.22–13.32 μM], kinetin [2.32–13.95 μM], Thidiazuron [TDZ, 0.45–4.54 μM])
and cytokinin in combination with auxins [naphthalene acetic acid (NAA, 0.54–5.37 μM)/indole acetic acid (IAA, 0.57–5.71 μM)/indole
butyric acid (IBA, 0.49–4.9 μM)]. All supplementary-induced shoot proliferation and the optimal results was on the medium
supplemented with 2.27 μM TDZ, which induced 5.1 shoots per explant. Among the different concentrations of sucrose (0–120 g
l−1) tested for shoot proliferation, 30 g l−1 was found suitable for corm cultures of Alocasia amazonica. The optimal shoot proliferation and biomass values were with the plantlets grown at 30 μmol m−2 s−1 photosynthetic photon flux (PPF) and 25°C. Liquid cultures found suitable for shoot proliferation and biomass accumulation
was compared to semisolid cultures. Comparative studies of bioreactor systems [continuous immersion (with or without net)
and temporary immersion in liquid media using ebb and flood] revealed that shoot multiplication and growth were greatest with
the raft bioreactor system. Plantlets (cormlets) from the bioreactor were hydroponically cultured for 30 days, and 100% of
plants were acclimatized successfully. The simple efficient method of production of plantlets (cormlets) is useful for large-scale
multiplication of this important ornamental plant.
An erratum to this article can be found at 相似文献
11.
Catapan Elizabete Otuki Michel Fleith Viana Ana Maria 《Plant Cell, Tissue and Organ Culture》2000,62(3):195-202
An efficient micropropagation protocol was developed for the medicinal plant Phyllanthus caroliniensis (Euphorbiaceae) using nodal segments for axillary shoot proliferation. Maximum multiplication (21–23 shoots per explant)
was achieved on MS or AR media supplemented with either 5.0 μM BA, 1.25–5.0 μM kinetin or 2.5–5.0 μM 2iP. Rooting was achieved
with 80–100% of the microshoots on MS medium without growth regulators, although 1.25 μM NAA and 1.25–5.0 μM IAA promoted
significant increases in the number of roots per explant. Regenerated plants were successfully acclimatized and about 88%
of plantlets survived under ex vitro conditions. Flowering was observed on in vitro grown plantlets and after 3–4 weeks of acclimatization. High frequency callus initiation and growth was achieved when nodal
segment explants were inoculated in the vertical position on MS medium supplemented with 5.0 μM 2,4-D. Root cultures were
successfully established on MS medium containing 1.1 μM NAA. The optimized micropropagation, callus and root culture protocols
offer the possibility to use cell/root culture techniques for vegetative propagation and secondary metabolism studies.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
12.
Giovanni Iapichino Marcello Airò 《In vitro cellular & developmental biology. Plant》2008,44(4):330-337
Multiple shoots were induced on stem segments of an 8-y-old plant of Metrosideros excelsa Sol ex Gaertn. “Parnel”. Axillary shoots produced on uncontaminated explants were excised, segmented, and recultured in the
same medium to increase the stock of shoot cultures. The Murashige and Skoog (MS) medium, augmented with different concentrations
of 2- isopenthenyladenine (2iP) and indole-3-acetic acid (IAA), either singly or in combinations, as potential medium for
shoot multiplication by nodal segments was tested. In the following experiment, equal molar concentrations of four cytokinins
[2iP, kinetin, zeatin, and N
6-benzyladenine (BA)] in combination with equal molar concentrations of three auxins [IAA, α-naphthaleneacetic acid (NAA),
and indole-3-butyric acid (IBA)] were tested for ability to induce axillary shoot development from single-node stem segments.
The highest rate of axillary shoot proliferation was induced on MS agar medium supplemented with 1.96μM 2iP and 1.14μM IAA
after 6 wk in culture. Different auxins (IAA, IBA, and NAA) were tested to determine the optimum conditions for in vitro rooting of microshoots. The best results were accomplished with IAA at 5.71μM (89% rooting) and with IBA at 2.85 or 5.71μM
(86% and 86% rooting, respectively). Seventy and 90 percent of the microshoots were rooted ex vitro in bottom-heated bench (22 ± 2°C) after 2 and 4 wk, respectively. In vitro and ex vitro rooted plantlets were successfully established in soil. 相似文献
13.
Summary An efficient and rapid micropropagation system was developed for a food and medicinally important endangered shrub, Decalepis hamiltonii (‘swallow root’), through shoot multiplication. The influence of 2.5–7.5 μM isopentenyladenine (2iP), 4.4–17.7 μM 6-benzyladenine, 2.3–4.7 μM kinetin, 2.8–6.8 μM thidiazuron, and 2.3–11.4 μM zeatin alone and in combination with 0.3–0.9 μM indole-3-acetic acid (IAA) on in vitro multiple shoot production was studied. The maximum number of multiple shoots (6.5±0.4) was induced from shoot tips cultured
on agar-based Murashige and Skoog (MS) medium containing 4.9 μM 2iP. But, both zeatin (9.1 μM) and kinetin (4.7 μM) in combination with IAA (0.6 μM) were able to produce a maximum of 5.0±0.4 and 5.1±0.4 multiple shoots, respectively. Further elongation of shoots and adventitious
shoot formation was obtained on medium containing 2.5 μM 2iP and 0.3 μM gibberellic acid. Elongated shoots were separated and rooted on MS medium supplemented with 9.8μM indole-3-butyric acid (IBA) and various phenolic compounds within 5–6 wk. Phloroglucinol and salicylic acid interaction with
IBA stimulated in vitro rooting of shoots. Successful field transfer was achieved in rooted plantlets. 相似文献
14.
Pranati Nayak P. R. Behera Thirunavoukkarasu Manikkannan 《In vitro cellular & developmental biology. Plant》2007,43(3):231-236
High frequency plantlet regeneration was achieved in cotyledonary nodes of Aegle marmelos. Cotyledonary nodes from 1 mo. old in vitro grown seedlings of A. marmelos were cultured on Murashige and Skoog (MS) medium supplemented with benzyl adenine (BA) (0–8.8 μM), kinetin (KIN) (0–9.4 μM),
and indole-3-acetic acid (IAA) (0–1.14 μM) either alone or in combinations. The highest regenerative response was observed
on medium containing 6.6 μM BA + 1.14 μM IAA where approximately 86.6% of the cultures responded with an average shoot numbers
of 487.5 per explant in 7-wk time. Cultures maintained on KIN-supplemented medium showed very poor response. In vitro responded shoots were transferred to root induction medium consisting of half-strength MS supplemented with auxins IAA, indole-3-butyric
acid (IBA), or α-naphthalene acetic acid (NAA). Rooting was best in medium supplemented with 14.7 μM IBA. Rooted plantlets
were acclimatized and transferred to the field with 80% survival rate. 相似文献
15.
Somatic embryos were induced from hypocotyl-derived callus of sesame (Sesamum indicum Var. TMV 6). The influence of different
auxins and cytokinins on somatic embryogenesis was investigated. Among the different auxins tested, 2,4-dichlorophenoxy acetic
acid was the most effective and resulted in the highest frequency of responding cultures and highest average number of somatic
embryo per responding cultures napthaleneacetic acid, indoleacetic acid, indolebutyric acid and 2,4,5-trichlorophenoxypropionic
acid were also effective for embryogenesis, but 2,4,5-trichorophenoxyacetic acid and napthoxyacetic acid were not beneficial.
The combined effect of cytokinins with 2,4-d was also studied. Among the four cytokinins tested, 2.2 chμM benzyladenine with
13.6 chμM 2,4-d slightly enhanced embryogenic efficiency; while kinetin, zeatin, 6-γ-γ-dimethylallylaminopurine enhanced the
frequency of responding cultures. There was a decrease in the number of somatic embryos per culture in the presence of all
cytokinins.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
16.
Effects of growth regulators and incubation period on in vitro regeneration of adventitious shoots from gerbera petioles 总被引:4,自引:0,他引:4
Orlikowska Teresa Nowak Elzbieta Marasek Agnieszka Kucharska Danuta 《Plant Cell, Tissue and Organ Culture》1999,59(2):95-102
An effective system for in vitro regeneration of adventitious shoots from callus for the transformation or mutation of gerbera was developed. Callus was produced
from petioles of the youngest 3–4 leaves detached from auxillary shoots produced in vitro. Induction medium, on which leaves were incubated over 3 or 6 days, contained 2.3 μM thidiazuron and 0.53 μM α-naphthaleneacetic
acid. Explants were than transferred to one of three regeneration media with lower levels of growth regulators. Regeneration
was quantified over four (4-weeks each) passages at the time of explant transfer to fresh medium. Direct shoot regeneration
occurred during the first 4 weeks, and after these shoots were discarded a semi-compact organogenic callus was produced. Effectiveness
of shoot regeneration depended on four criteria: the cultivar (three cultivars were tested), the sequence of passage on regeneration
medium, the growth regulators in regeneration medium and the duration of the induction period. Regeneration potential from
calli of all cultivars increased from the first to the fourth passage. Duration of the incubation period on induction medium
(3 or 6 days) influenced regeneration to varying degrees, depending on the cultivar used and the regeneration medium contents.
There were no differences between two of the regeneration media – B, containing 2.2 μM 6-benzyladenine and 0.3 μM indole-3-acetic
acid and C, containing 4.4 μM 6-benzyladenine, 4.6 μM zeatin and 0.6 μM indole-3-acetic acid. Cultivar Mariola was the most
productive and regenerated more than seven shoots per callus in the fourth passage. Regeneration on medium B was further evaluated
on four additional gerbera cultivars.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
17.
An efficient in vitro regeneration protocol was developed for medicinally important aromatic plant Anethum graveolens. Nodal segments were cultured onto Murashige and Skoog (MS) basal medium supplemented with different auxins and cytokinins
singly as well as in combinations. The optimum callus induction (93.33 %) was obtained on medium fortified with 2.2 μM N6-benzyladenine (BA) and 0.21 μM α-naphthaleneacetic acid. The best shoot regeneration (85.7 %) with 12.86 shoots per explant
was achieved in two weeks when callus was subcultured on MS medium amended with 2.2 μM BA and 1.85 μM kinetin. The average
length of regenerated shoots varied from 3.15 to 4.8 cm. The rooting of regenerated shoots was nearly 100 % on ? MS augmented
with 4.9 μM indolebutyric acid with a maximum root length of 5.1 cm. Plantlets were successfully acclimatized with 60 % survival
rate. During organogenesis, catalase and ascorbate peroxidase activity increased while superoxid dismutase activity decreased.
Clonal fidelity of in vitro raised plants has been checked by random amplified polymorphic DNA using 10 selected decamer primers. It has been found that
regenerated plants are true to type plants. 相似文献
18.
Gopal J. Chamail Anjali Sarkar Debabrata 《In vitro cellular & developmental biology. Plant》2004,40(5):485-490
Summary With the objective of using microtubers for conservation of potato germplasm, the main effects of genotype, abscisic acid
(ABA), and sucrose level, and of their interactions on biomass production, microtuberization, microtuber dormancy, and dry
matter content, were studied. ABA decreased both microtuber production and microtuber dormancy, whereas higher concentrations
(60–80 gl−1) of sucrose promoted biomass production, microtuber production as well as microtuber dry matter content. Microtubers stored
under diffused light had longer dormancy than those kept continuously in the dark. Interactions among various factors conditioned
the main effects for some characters. In vitro performance of the genotypes studied was related to their known performance under in vivo conditions for most of the characters. Microtubers produced on media devoid of ABA and containing high sucrose concentrations
and N6-benzyladenine (44.38 μM) could be stored for 12 mo. under diffused light at 6±1°C. 相似文献
19.
Scott P. Burns Maria Gallo Barry L. Tillman 《In vitro cellular & developmental biology. Plant》2012,48(1):58-66
Agrobacterium-mediated transformation, employing direct shoot organogenesis, allows for mature transgenic plants to be obtained quickly
(3–4 mo). In this study, peanut (Arachis hypogaea L.) cultivars Florida-07, Georgia Green, Georgia Brown, New Mexico Valencia A, and VC-2 were selected to test their shoot
induction response for use in future transformation experiments. Two types of cotyledon explants were examined, those that
previously had an attached embryo axis upon cotyledon separation (explant A) and those that were embryo axis-free upon separation
(explant B). Explants were placed onto a shoot induction medium with N
6-benzyladenine concentrations ranging from 10–80 μM for Florida-07, Georgia Green, and VC-2; 10–20 μM for Georgia Brown; and
10–640 μM for New Mexico Valencia A. Following a 4-wk culture period, explants were visually rated based on a scale of 1–4,
where 1 indicated slight greening, but no growth, and 4 indicated greening, adventitious bud formation, as well as small leaf
expansion. A difference in shoot induction was observed for the cotyledon explants examined (P > t = <0.0001). Explant A had greater shoot induction with a visual rating of 1.8 ± 0.1; explant B had a rating of 1.6 ± 0.1
(P > t = <0.0001). Additionally, cultivars responded to the culture conditions differently (cultivar × N
6-benzyladenine interaction). Georgia Green on 10 μM N
6-benzyladenine produced the most shoot buds (24.6%) and the highest visual rating (2.1), followed by VC-2 on 10 μM N
6-benzyladenine (22.1%, 1.8), New Mexico Valencia A on 640 μM N
6-benzyladenine (21.4%, 1.8), Georgia Brown on 80 μM N
6-benzyladenine (9.0%, 1.7), and Florida-07 on 40 μM N
6-benzyladenine (7.1%, 1.8). Of the tested varieties, Georgia Green, New Mexico Valencia A, and VC-2 were best suited for future
transformation experiments based on their shoot bud production. 相似文献
20.
Summary A method of plant regeneration from hypocotyl segments of Platanus acerifolia Willd, has been developed. Hypocotyl slices were cultured on Murashige and Skoog (MS) basal medium supplemented with a range
of combinations of cytokinins [6-benzyladenine (BA) or kinetin] and auxins [indole-3-butyric acid (IBA), indole-3-acetic acid,
α-naphthaleneacetic acid or 2,4-dichlorophenoxyacetic acid] for adventitious shoot induetion. The highest regeneration frequency
was obtained with MS medium containing 2.0 mg l−1 (8.88 μM) BA and 0.5 mg l−1 (2.46 μM) IBA. Adventitious buds and shoots were differentiated from hypocotyl-derived cellus or directly from the wounded sites within
4–8 wk. The regenerated shoots were elongated and proliferated efficiently on multiplication medium. Complete plantlets were
transplanted to the soil and grew normally in the greenhouse after root formation on rooting medium for 4–6 wk. 相似文献