CULTURE AND NUTRITION OF AMOEBIDIUM PARASITICUM

Whisler , Howard C. (McGill U., Montreal, Canada.) Culture and nutrition of Amoebidium parasiticum. Amer. Jour. Bot. 49(3): 193–199. Illus. 1962.—The little known Trichomycete, Amoebidium parasiticum, has been isolated into pure culture Maximum growth was obtained on a thiamine-enriched Tryptone-glucose medium. Growth also occurred on a defined medium consisting of thiamine, glucose, methionine, ammonium and inorganic salts. Methionine could not be replaced by sulfate or l -cystine. Both mannose and fructose could satisfy the carbon requirement. At present, reproduction in pure culture is always by sporangiospores, but the amoeboid phase can be induced by the addition of crushed host material.