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1.
M. L. Xu S. S. Korban 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2000,101(5-6):844-851
Using the amplified fragment length polymorphism (AFLP) technique combined with a ”narrow-down” bulk segregant strategy enabled
us to quickly identify 15 tightly linked AFLP markers to the Vf gene that confers resistance to the apple scab disease. High-resolution mapping placed all 15 AFLP markers within an interval
of 0.6 cM around the Vf region; 7 of them were found to be inseparable from the Vf gene, 1 was localized left of, and the remaining 7 located right of the Vf gene. In addition, eight previously identified RAPD markers were also mapped, but only three, including M18, AM19, and AL07,
were localized within this short interval, and none co-segregated with the Vf gene. The saturation of the Vf region with AFLP markers will accelerate both marker-assisted selection and map-based cloning. The advantages of this ”narrow-down”
strategy, estimation of physical distances among AFLP markers, and their potential application are also discussed.
Received: 22 December 1999 / Accepted: 25 March 2000 相似文献
2.
A. Singh M. S. Negi J. Rajagopal S. Bhatia U. K. Tomar P. S. Srivastava M. Lakshmikumaran 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,99(1-2):272-279
Genetic diversity was estimated in 37 neem accessions from different eco-geographic regions of India and four exotic lines
from Thailand using AFLP markers. Seven AFLP selective primer combinations generated a total of 422 amplification products.
The average number of scorable fragments was 60 per experiment, and a high degree (69.8%) of polymorphism was obtained per
assay with values ranging from 58% to 83.8%. Several rare and accession-specific bands were identified which could be effectively
used to distinguish the different genotypes. Genetic relationships within the accessions were evaluated by generating a similarity
matrix based on the Jaccard index. The phenetic dendrogram generated by UPGMA as well as principal correspondence analysis
separated the 37 Indian genotypes from the four Thai lines. The cluster analysis indicated that neem germplasm within India
constitutes a broad genetic base with the values of genetic similarity coefficient ranging from 0.74 to 0.93. Also, the Indian
genotypes were more dispersed on the principal correspondence plot, indicating a wide genetic base. The four lines from Thailand,
on the other hand, formed a narrow genetic base with similarity coefficients ranging from 0.88 to 0.92. The lowest genetic
similarity coefficient value (0.47) was observed between an Indian and an exotic genotype. The level of genetic variation
detected within the neem accessions with AFLP analysis suggests that it is an efficient marker technology for delineating
genetic relationships amongst genotypes and estimating genetic diversity, thereby enabling the formulation of appropriate
strategies for conservation and tree improvement programs.
Received: 20 October 1998 / Accepted: 28 November 1998 相似文献
3.
Molecular markers linked to stem rot resistance in rice 总被引:4,自引:0,他引:4
J. Ni P. M. Colowit J. J. Oster K. Xu D. J. Mackill 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2001,102(4):511-516
Stem rot (Sclerotium oryzae) is an important disease constraint in Californian rice production. Measurement of resistance is laborious, and the low heritability
of the trait limits the effectiveness of selection in breeding programs. Molecular markers linked to the trait would therefore
provide a superior selection screen to assist in transferring resistance into improved cultivars. The genetics of resistance
to stem rot was studied in the germplasm line 87-Y-550 (PI566666), which inherited its resistance from the wild species Oryza rufipogon. Four crosses of 87-Y-550 with susceptible lines were made and recombinant inbred lines of only the most-resistant and most-susceptible
progeny within each cross were advanced for late-generation testing. Approximately 900 AFLP (amplified fragment length polymorphism)
primer combinations were applied to resistant and susceptible bulks within each cross. One AFLP marker showed significant
association with stem rot resistance and accounted for approximately 45.0% of the phenotypic variation in 59 progenies. This
marker was mapped on rice chromosome 2 between the RFLP markers RZ166 and RG139 by using F2-reference population information. The accuracy of AFLP marker mapping was validated by size and sequence comparison of AFLP
bands from 87-Y-550 and the reference population. With the strategy of selective genotyping combined with a parental survey,
two microsatellite markers, RM232 and RM251, on chromosome 3 were also found associated with stem rot resistance and accounted
for 41.1% and 37.9% of the phenotypic variation, respectively. The multiple linear regression model included TAA/GTA167 on
chromosome 2 and RM232 on chromosome 3 and cumulatively explained 49.3% of total variation. The molecular markers linked to
stem rot resistance should facilitate selection for this recalcitrant trait in rice breeding programs by eliminating the need
for early generation screening.
Received: 27 March 2000 / Accepted: 4 June 2000 相似文献
4.
Assessment of genetic diversity in a Morus germplasm collection using fluorescence-based AFLP markers 总被引:6,自引:0,他引:6
A. Sharma R. Sharma H. Machii 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2000,101(7):1049-1055
To meet various breeding objectives and to conserve the existing genetic resources of mulberry for future use, the present
study was undertaken to investigate the amount of genetic diversity and to establish the relationships between mulberry genotypes
using fluorescence-based AFLP markers. Genetic diversity was estimated in 45 mulberry accessions from different eco-geographic
regions of Japan and other parts of the world. Five primer combinations amplified an average of 110 AFLP markers per primer
combination, ranging in size from 35 to 500 bp. A high degree of polymorphism was revealed by these combinations that ranged
from 69.7 to 82.3% across all the genotypes studied. Several rare genotype-specific bands were also identified which could
be effectively utilized to distinguish different genotypes. The wide range in genetic similarity coefficients (0.58–0.99)
indicated that the mulberry germplasm collection represents a genetically diverse popu-lation. The phenetic dendrogram generated
by the UPGMA method grouped 45 accessions into four major clusters, which was in agreement with the results from conventional
methods. Clustering of some genotypes into strictly separate groups was not readily apparent and no clear interrelationships
could be depicted, in spite of their different geographic origin. In addition, AFLP analysis provided sufficient polymorphism
for DNA typing and contributed additional insights into the genetic structure of the mulberry germplasm. These results will
help in the formulation of appropriate strategies for conservation and variety improvement in mulberry, for which little or
no knowledge of genetic diversity is currently available.
Received: 30 December 1999 / Accepted: 14 March 2000 相似文献
5.
Polymorphism, distribution, and segregation of AFLP markers in a doubled haploid rice population 总被引:18,自引:0,他引:18
M. Maheswaran P. K. Subudhi S. Nandi J. C. Xu A. Parco D. C. Yang N. Huang 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1997,94(1):39-45
We exploited the newly developed amplified fragment length polymorphism (AFLP) technique to study the polymorphism, distribution
and inheritance of AFLP markers with a doubled haploid rice population derived from ‘IR64’/‘Azucena’. Using only 20 pairs
of primer combinations, we detected 945 AFLP bands of which 208 were polymorphic. All 208 AFLP markers were mapped and distributed
over all 12 chromosomes. When these were compared with RFLP markers already mapped in the population, we found the AFLP markers
to be highly polymorphic in rice and to follow Mendelian segregation. As linkage map of rice can be generated rapidly with
AFLP markers they will be very useful for marker-assisted backcrossing.
Received: 11 April 1996 / Accepted: 14 June 1996 相似文献
6.
Evaluation of genetic variation in the daylily (Hemerocallis spp.) using AFLP markers 总被引:1,自引:0,他引:1
J. P. Tomkins T. C. Wood L. S. Barnes A. Westman R. A. Wing 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2001,102(4):489-496
The daylily (Hemerocallis spp.) is one of the most economically important ornamental plant species in commerce. Interestingly, it is also one of the
most heavily bred crops during the past 60 years. Since the American Hemerocallis Society began acting as the official registry
of daylily cultivars in 1947, more than 40 000 registrations have been processed. In order to determine the effects of intensive
breeding on cultivar development, and to study relationships among different species, genetic variation in the daylily was
estimated using AFLP markers. Nineteen primary genotypes (species and early cultivars) and 100 modern cultivars from different
time periods were evaluated using 152 unambiguous bands (average 79% polymorphism rate) derived from three AFLP primer combinations.
Overall, pairwise similarity estimates between entries ranged between 0.618 and 0.926 (average=0.800). When comparing cultivar
groups from different time periods (1940–1998), genetic similarity was initially increased, compared to the primary diploid
genotypes, remained constant from 1940 to 1980, and then steadily increased as breeding efforts intensified and hybridizers
began focusing on a limited tetraploid germplasm pool derived by colchicine conversion. Among modern (1991–1998) daylily cultivars,
genetic similarity has increased by approximately 10% compared to the primary genotypes. These data were also used to evaluate
recent taxonomic classifications among daylily species which, with a few minor exceptions, were generally supported by the
AFLP data.
Received: 15 March 2000 / Accepted: 13 June 2000 相似文献
7.
Porceddu A Albertini E Barcaccia G Falistocco E Falcinelli M 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2002,104(2-3):273-280
The high versatility of the mode of reproduction and the retention of a pollen recognition system are the factors responsible
for the extreme complexity of the genome in Poa pratensis L. Two genetic maps, one of an apomictic and one of a sexual genotype, were constructed using a two-way pseudo-testcross
strategy and multiplex PCR-based molecular markers (AFLP and SAMPL). Due to the high ploidy level and the uncertainty of chromosome
pairing-behavior at meiosis, only parent-specific single-dose markers (SDMs) that segregated 1:1 in an F1 mapping population (161 out of 299 SAMPLs, and 70 out of 275 AFLPs) were used for linkage analysis. A total of 41 paternal
(33 SAMPLs and 8 AFLPs) and 47 maternal (33 SAMPLs and 14 AFLPs) SDMs, tested to be linked in coupling phase, were mapped
to 7+7 linkage groups covering 367 and 338.4 cM, respectively. The comparison between the two marker systems revealed that
SAMPL markers were statistically more efficient than AFLP ones in detecting parent-specific SDMs (75% vs 32.4%). There were
no significant differences in the percentages of distorted marker alleles detected by the two marker systems (27.8% of SAMPLs
vs 21.3% of AFLPs). The pairwise comparison of co-segregational groups for linkage detection between marker loci suggested
that at least some of the P. pratensis chromosomes pair preferentially at meiosis-I.
Received: 31 August 2000 / Accepted: 12 January 2001 相似文献
8.
Towards a saturated sorghum map using RFLP and AFLP markers 总被引:17,自引:3,他引:17
K. Boivin M. Deu J.-F. Rami G. Trouche P. Hamon 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,98(2):320-328
A near-saturated sorghum genetic linkage map was produced using RFLP, AFLP and morphological markers. First a composite,
essentially RFLP-based genetic linkage map was obtained from analyses of two recombinant inbred populations. This map includes
343 loci for 11 linkage groups spanning 1352 cM. Since this map was constructed with many previously mapped heterologous probes,
it offers a good basis for synteny studies. Separately, an AFLP map was obtained from the analysis of 168 bands revealed from
12 primer pair combinations. It includes 137 loci for 11 linkage groups spanning 849 cM. Taking into account the different
data sets, we constructed a combined genetic linkage map including 443 loci spanning 1899 cM. Two main features are to be
noted: (1) the distribution of AFLPs along the genome is not uniform; (2) an important stretching of the former core map is
induced after adding the AFLPs.
Received: 10 May 1998 / Accepted: 13 July 1998 相似文献
9.
J. Garcia-Mas M. Oliver H. Gómez-Paniagua M.C. de Vicente 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2000,101(5-6):860-864
Three different types of molecular markers, RAPD, AFLP and RFLP were used to measure genetic diversity among six genotypes
of Cucumis melo L. Each line represented a different melon genotype: Piel de Sapo, Ogen, PI161375, PI414723, Agrestis and C105. A number
of polymorphic RAPD, AFLP and RFLP bands were scored on all materials and the genetic similarity measured. Clustering analysis
performed with the three types of markers separated the genotypes into two main groups: (1) the sweet type, cultivated melons
and (2) the exotic type, not cultivated melons. While the data obtained suggest that all three types of markers are equally
informative, AFLPs showed the highest efficiency in detecting polymorphism.
Received: 30 December 1999 / Accepted: 24 January 2000 相似文献
10.
P. F. Bert G. Charmet P. Sourdille M. D. Hayward F. Balfourier 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,99(3-4):445-452
AFLP markers have been successfully employed for the development of a high-density linkage map of ryegrass (Lolium
perenne L.) using a progeny set of 95 plants from a testcross involving a doubled-haploid tester. This genetic map covered 930 cM
in seven linkage groups and was based on 463 amplified fragment length polymorphism (AFLP) markers using 17 primer pairs,
three isozymes and five EST markers. The average density of markers was approximately 1 per 2.0 cM. However, strong clustering
of AFLP markers was observed at putative centromeric regions. Around these regions, 272 markers covered about 137 cM whereas
the remaining 199 markers covered approximately 793 cM. Most genetic distances between consecutive pairs of markers were smaller
than 20 cM except for five gaps on groups A, C, D, F and G. A skeletal map with a uniform distribution of markers can be extracted
from this high-density map, and can be applied to detect and map QTLs. We report here the application of AFLP markers to genome
mapping, in Lolium as a prelude to quantitative trait locus (QTL) identification for diverse agronomic traits in ryegrass and for marker-assisted
plant breeding.
Received: 4 November 1998 / Accepted:15 March 1999 相似文献
11.
Molecular mapping of a rice gene conditioning thermosensitive genic male sterility using AFLP, RFLP and SSR techniques 总被引:19,自引:0,他引:19
N. V. Dong P. K. Subudhi P. N. Luong V. D. Quang T. D. Quy H. G. Zheng B. Wang H. T. Nguyen 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2000,100(5):727-734
The discovery and application of the thermosensitive genic male sterility (TGMS) system has great potential for revolutionizing
hybrid seed production technology in rice. Use of the TGMS system in two-line breeding is simple, inexpensive, efficient,
and eliminates the limitations associated with the cytoplasmic-genetic male sterility (CMS) system. An F2 population developed from a cross between a TGMS indica mutant, TGMS–VN1, and a fertile indica line, CH1, was used to identify molecular markers linked to the TGMS gene and to subsequently determine its chromosomal location
on the linkage map of rice. Bulk segregant analysis was performed using the AFLP technique. From the survey of 200 AFLP primer
combinations, four AFLP markers (E2/M5–600, E3/M16–400, E5/M12–600, and E5/M12–200) linked to the TGMS gene were identified.
All the markers were linked to the gene in the coupling phase. All except E2/M5–200 were found to be low-copy sequences. However,
the marker E5/M12–600 showed polymorphism in RFLP analysis and was closely linked to the TGMS gene at a distance of 3.3 cM.
This marker was subsequently mapped on chromosome 2 using doubled-haploid mapping populations derived from the crosses IR64×Azucena
and CT9993×IR62666, available at IRRI, Philippines, and Texas Tech University, respectively. Linkage of microsatellite marker
RM27 with the TGMS gene further confirmed its location on chromosome 2. The closest marker, E5/M12–600, was sequenced so that
a PCR marker can be developed for the marker-assisted transfer of this gene to different genetic backgrounds. The new TGMS
gene is tentatively designated as tms4(t).
Received: 13 July 1999 / Accepted: 27 July 1999 相似文献
12.
AFLP markers tightly linked to the aluminum-tolerance gene Alt3 in rye (Secale cereale L.) 总被引:3,自引:0,他引:3
Miftahudin G. J. Scoles J. P. Gustafson 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2002,104(4):626-631
Rye (Secale cereale L.) is considered to be the most aluminum (Al)-tolerant species among the Triticeae. It has been suggested that aluminum
tolerance in rye is controlled by three major genes (Alt genes) located on rye chromosome arms 3RL, 4RL, and 6RS, respectively. Screening of an F6 rye recombinant inbred line (RIL) population derived from the cross between an Al-tolerant rye (M39A-1–6) and an Al-sensitive
rye (M77A-1) showed that a single gene controls aluminum tolerance in the population analyzed. In order to identify molecular
markers tightly linked to the gene, we used a combination of amplified fragment length polymorphism (AFLP) and bulked segregant
analysis techniques to evaluate the F6 rye RIL population. We analyzed approximately 22,500 selectively amplified DNA fragments using 204 primer combinations and
identified three AFLP markers tightly linked to the Alt gene. Two of these markers flanked the Alt locus at distance of 0.4 and 0.7 cM. Chromosomal localization using cloned AFLP and a restriction fragment length polymorphism
(RFLP) marker indicated that the gene was on the long arm of rye chromosome 4R. The RFLP marker (BCD1230) co-segregated with
the Alt gene. Since the gene is on chromosome 4R, the gene was designated as Alt3. These markers are being used as a starting point in the construction of a high resolution map of the Alt3 region in rye.
Received: 29 March 2000 / Accepted: 9 July 2001 相似文献
13.
Development of AFLP markers in barley 总被引:36,自引:0,他引:36
To investigate the application of amplified fragment length polymorphism (AFLP) markers in barley, 96 primer combinations
were used to generate AFLP patterns with two barley lines, L94 and Vada. With seven primer combinations, only a few intense
bands were obtained, probably derived from repeated sequences. With the majority of the remaining 89 primer combinations,
on average about 120 amplification products were generated, and the polymorphism rate between the two lines was generally
over 18%. Based on the number of amplified products and the polymorphism rate, the 48 best primer combinations were selected
and tested on 16 barley lines, again including L94 and Vada. Using a subset of 24 primer combinations 2188 clearly visible
bands within the range from 80 to 510 bp were generated; 55% of these showed some degree of polymorphism among the 16 lines.
L94 versus Vada showed the highest polymorphism rate (29%) and Proctor versus Nudinka yielded the lowest (12%). The polymorphism
rates per primer combination showed little dependence on the barley lines used. Hence the most efficient and informative primer
combinations identified for a given pair of lines turned out to be highly efficient when applied to others. Generally, more
than 100 common markers (possibly locus specific) among populations or crosses were easily identified by comparing 48 AFLP
profiles of the parent lines. The existence of such a large number of markers common to populations will facilitate the merging
of molecular marker data and other genetic data into one integrated genetic map of barley.
Received: 28 October 1996 / Accepted: 27 November 1996 相似文献
14.
Estimation of outcrossing rate in a breeding population of Eucalyptus urophylla with dominant RAPD and AFLP markers 总被引:7,自引:0,他引:7
F. A. Gaiotto M. Bramucci D. Grattapaglia 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1997,95(5-6):842-849
Eucalyptus breeding is typically conducted by selection in open-pollinated progenies. As mating is controlled only on the female side
of the cross, knowledge of outcrossing versus selfing rates is essential for maintaining adequate levels of genetic variability
for continuous gains. Outcrossing rate in an open-pollinated breeding population of Eucalyptus urophylla was estimated by two PCR-based dominant marker technologies, RAPD and AFLP, using 11 open-pollinated progeny arrays of 24
individuals. Estimated outcrossing rates indicate predominant outcrossing and suggest maintenance of adequate genetic variability
within families. The multilcous outcrossing rate (tm) estimated from RAPD markers (0.93±0.027), although in the same range, was higher (α>0.01) than the estimate based on AFLP
(0.89±0.033). Both estimates were of similar magnitude to those estimated for natural populations using isozymes. The estimated
Wright’s fixation index was lower than expected based on tm possibly resulting from selection against selfed seedlings when sampling plants for the study. An empirical analysis suggests
that 18 is the minimum number of dominant marker loci necessary to achieve robust estimates of tm. This study demonstrates the usefulness of dominant markers, both RAPD and AFLP, for estimating the outcrossing rate in breeding
and natural populations of forest trees. We anticipate an increasing use of such PCR-based technologies in mating-system studies,
in view of their high throughput and universality of the reagents, particularly for species where isozyme systems have not
yet been optimized.
Received: 25 March 1997 / Accepted: 13 May 1997 相似文献
15.
Phylogenetic relationships among Oryza species revealed by AFLP markers 总被引:15,自引:0,他引:15
R. K. Aggarwal D. S. Brar S. Nandi N. Huang G. S. Khush 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,98(8):1320-1328
The genus Oryza to which cultivated rice belongs has 22 wild species. Seventy-seven accessions of 23 Oryza species, five related genera, and three outgroup taxa were fingerprinted using amplified fragment length polymorphism (AFLP).
A total of 1191 polymorphic markers were obtained using five AFLP primer combinations. AFLP data were analyzed to study species
relationships using different clustering algorithms, and the resulting phenograms were tested for stability and robustness.
The findings suggest a common ancestry to the genus Oryza. Moreover, the results demonstrate that: (1) evolution in Oryza has followed a polyphyletic path wherein multiple lineages underwent independent divergence after separation early in the
evolution from a common ancestor/pool of related taxa; (2) newly assigned genomes, GG for O. meyeriana and HHJJ for O. ridleyi complexes, are among the most diverged in the genus; (3) CCDD tetraploids have a relatively ancient origin among the Officinalis
complex; (4) O. malampuzhaensis, O. indandamanica, O. alta, and O. grandiglumis are diverged enough to deserve species status; (5) O. officinalis and O. eichingeri (CC) are putative progenitors of O. minuta
*
O. malampuzhaensis and tetraploid O. punctata, respectively, (6) O. brachyantha is most diverged species in the genus. AFLP is reliable molecular technique and provides one of the most informative approaches
to ascertain genetic relationships in Oryza, which may also be true for other related species/organisms.
Received: 1 July 1998 / Accepted: 2 November 1998 相似文献
16.
S. Garland L. Lewin A. Blakeney R. Reinke R. Henry 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2000,101(3):364-371
The genomic DNA clone RG28, linked to the major fragrance gene of rice (fgr), was assessed for polymorphism in order to produce a PCR-based marker for fragrance. A small mono-nucleotide repeat, that
was polymorphic between a pair of fragrant and non-fragrant cultivars, was identified and developed into a co-dominant PCR-based
marker. The polymorphism-information-content determinations for three microsatellite markers, that have been genetically mapped
near RG28, are also presented. These PCR-based markers will be highly useful in distinguishing fragrance-producing alleles
from non-fragrance-producing alleles at the fgr locus.
Received: 19 October 1999 / Accepted: 16 December 1999 相似文献
17.
Linkage map of Japanese black pine based on AFLP and RAPD markers including markers linked to resistance against the pine needle gall midge 总被引:6,自引:0,他引:6
E. Hayashi T. Kondo K. Terada N. Kuramoto Y. Goto M. Okamura H. Kawasaki 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2001,102(6-7):871-875
Macrogametophytes derived from the seeds of a tree resistant to pine needle gall midge (PGM) were analyzed using amplified
fragment length polymorphism (AFLP). A total of 244 segregating loci were detected among 71 macrogametophytes. Combining the
AFLP results with previously reported segregation data for 127 random amplified polymorphic DNA (RAPD) markers, 157 AFLP and
50 RAPD markers with confirmed map positions were assigned to 20 linkage groups and three pairs covering 2085.5 cM with an
average distance of 10.1 cM. The total map distance covers about 77.1–78.4% of the total genome, estimated to be approximately
2665–2719 cM in length. Thus, using AFLP markers, the previous RAPD map of this tree was improved in terms of the average
distance between markers, the total map distance, and coverage of the genome. Three RAPD markers linked to a gene associated
with resistance to PGM were also located on this map.
Rceived: 14 April 2000 / Accepted: 21 August 2000 相似文献
18.
Integrated map of AFLP, SSLP and RFLP markers using a recombinant inbred population of rice (Oryza sativa L.) 总被引:7,自引:0,他引:7
Y. G. Cho S. R. McCouch M. Kuiper M.-R. Kang J. Pot J. T. M. Groenen M. Y. Eun 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,97(3):370-380
A molecular map of rice consisting of 231 amplified fragment length polymorphisms (AFLPs), 212 restriction fragment length
polymorphisms (RFLPs), 86 simple-sequence length polymorphisms (SSLPs), five isozyme loci, and two morphological mutant loci
[phenol staining of grain (Ph), semi-dwarf habit (sd-1)] has been constructed using an F11 recombinant inbred (RI) population. The mapping population consisted of 164 RI lines and was developed via single-seed descent
from an intercross between the genetically divergent parents Milyang 23 (M) (tongil type) and Gihobyeo (G) ( japonica type). A subset of previously mapped RFLP and SSLP markers were used to construct the map framework. The AFLP markers were
derived from ten EcoRI(+2) and MseI(+3) primer combinations. All marker types were well distributed throughout the 12 chromosomes. The integrated map covered
1814 cM, with an average interval size of 3.4 cM. The MG map is a cornerstone of the Korean Rice Genome Research Program (KRGRP)
and is being continuously refined through the addition of partially sequenced cDNA markers derived from an immature-seed cDNA
library developed in Korea, and microsatellite markers developed at Cornell. The population is also being used for quantitative
trait locus (QTL) analysis and as the basis for marker-assisted variety development.
Received: 24 June 1997 / Accepted: 25 November 1997 相似文献
19.
X. Shan T. K. Blake L. E. Talbert 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,98(6-7):1072-1078
Conversion of amplified fragment length polymorphisms (AFLPs) to sequence-specific PCR primers would be useful for many genetic-linkage
applications. We examined 21 wheat nullitetrasomic stocks and five wheat-barley addition lines using 12 and 14 AFLP primer
combinations, respectively. On average, 36.8% of the scored AFLP fragments in the wheat nullitetrasomic stocks and 22.3% in
the wheat-barley addition lines could be mapped to specific chromosomes, providing approximately 461 chromosome-specific AFLP
markers in the wheat nullitetrasomic stocks and 174 in the wheat-barley addition lines. Ten AFLP fragments specific to barley
chromosomes and 16 AFLP fragments specific to wheat 3BS and 4BS chromosome arms were isolated from the polyacrylamide gels,
re-amplified, cloned and sequenced. Primer sets were designed from these sequences. Amplification of wheat and barley genomic
DNA using the barley derived primers revealed that three primer sets amplified DNA from the expected chromosome, five amplified
fragments from all barley chromosomes but not from wheat, one amplified a similar-sized fragment from multiple barley chromosomes
and from wheat, and one gave no amplification. Amplification of wheat genomic DNA using the wheat-derived primer sets revealed
that three primer sets amplified a fragment from the expected chromosome, 11 primer sets amplified a similar-sized fragment
from multiple chromosomes, and two gave no amplification. These experiments indicate that polymorphisms identified by AFLP
are often not transferable to more sequence-specific PCR applications.
Received: 30 June 1998 / Accepted: 26 October 1998 相似文献
20.
The systematic evaluation of the molecular diversity encompassed in Aconitum kongboense L. inbreds or parental lines offers an efficient means of exploiting the heterosis in A. kongboense as well as for management of biodiversity. An excellent and novel DNA-based molecular, amplified fragment length polymorphisms (AFLPs) markers, was firstly used to analysis the genetic diversity in A. kongboense genotypes. Out of 256 primers screened, a total of ten combinations successfully produced scorable, clear, reproducible and relatively high polymorphism bands, 64.12% of which were polymorphic. The values of number of polymorphic loci (NPL), percentage of polymorphic loci (PPB), observed number of alleles (Na) and effective number of alleles (Ne) were highest in population 3 (NPL = 77, PPB = 68.75%, Na = 1.688 ± 0.466, Ne = 1.412 ± 0.397) and lowest in population 2 (NPL = 57, PPB = 50.89%, Na = 1.509 ± 0.502, Ne = 1.273 ± 0.340). In addition, Jaccard's similarity coefficients among different populations varied from 0.45 to 1.00 with an average of 0.55. The data collected will contribute to identification, rational exploitation and conservation of germplasms of A. kongboense, and potentially useful to aid its breeding. 相似文献