共查询到20条相似文献,搜索用时 15 毫秒
1.
Myb genes from Hordeum vulgare: tissue-specific expression of chimeric Myb promoter/Gus genes in transgenic tobacco 总被引:2,自引:0,他引:2
Margit Wissenbach Bärbel Überlacker Friederike Vogt Dieter Becker Francesco Salamini Wolfgang Rohde 《The Plant journal : for cell and molecular biology》1993,4(3):411-422
The structures of the three Myb -related genes Hv1 , Hv5 and Hv33 from barley were determined. They contain a single intron located in the second repeat unit of the Myb -related domain. By analogy to the animal MYB oncoproteins this conserved region of the gene product was shown by filter-binding experiments to exhibit nucleic acid-binding activity. Tobacco plants transgenic for chimeric Myb promoter/ Gus genes express the enzyme in a developmentally controlled and tissue-specific manner. During germination and early stages of plant growth, GUS activity is seen in the root cap and adjacent meristematic tissue. At later stages of plant development, GUS activity is predominantly observed in the shoot apical meristem, the roots and the nodal regions of the stem. Within the stem at stages of secondary growth, Myb promoters are active in defined cell types. In the internode low GUS activity is displayed by the innermost cell layer of the cortex, the starch sheath, that surrounds the vascular cylinder of secondary xylem and phloem tissue, as well as in pith rays originating from vascular cambium initials. In the nodal region Myb promoter-controlled Gus expression is mainly confined to the abaxial starch sheath of the leaf trace, to the branch traces and to internal strands of primary phloem. It is suggested that in addition to their activity in meristematically active plant tissues Myb genes are expressed in conductive tissues that are closely associated with vascular bundles. 相似文献
2.
Jack Maze 《American journal of botany》1977,64(5):504-515
The vascular bundles in the inflorescence axis of Andropogon gerardii occur in inner and outer systems. The inner system is made up of large, early developing strands that, at earliest stages of development, are precocious (= the appendage they are to serve has not yet been initiated). The outer system consists of later developing smaller strands that are open ended in a proximal direction (= strands differentiate basipetally in the cortex below the appendage they serve). Bundles of both the inner and outer systems are not connected to other procambium early in their development but exist as isolated strands. The veins of the inner system of the inflorescence axis occur as sympodia. The presence of inner and outer systems in the vascular tissue is common to most monocotyledons. However, amongst monocotyledons, only certain grasses have been shown to have strands of the inner system that are isolated early in development. Many dicotyledons have large strands which are precocious and some have smaller, later developing strands which are open ended in a proximal direction, hence they occur as isolated strands. These smaller strands in dicotyledons occur between large strands. Certain dicotyledons have an inner and an outer system of veins. Of these, some have veins of the inner system that differ from the inner system bundles of monocotyledons in that they also form part of the outer system of veins, or develop at a different time. One other dicotyledon with an inner and outer system, Bougainvillea, differs from monocotyledons only in that the bundles of the outer system do not seem to be isolated early in their development and anastomoses are seen between the inner and outer systems. Thus, it appears that monocotyledons differ from dicotyledons only in the presence of independent inner and outer systems of vascular bundles in the former. Thus, the hypothesis of Zimmermann and Tomlinson that there are basic differences between monocotyledon and dicotyledon vascular systems is not substantiated. It is even suspected that monocotyledon and dicotyledon vascular systems will be demonstrated to be modifications of a basic plan consisting of large, acropetally differentiating and smaller, basipetally differentiating strands. 相似文献
3.
Polarity and the Induction of Organized Vascular Tissues 总被引:5,自引:0,他引:5
This work deals with those properties of plant tissues whichare responsible for the organization of vascular cells in orderedstrands. It is shown that auxin alone is sufficient to causethe differentiation of strands of xylem cells in the parenchymaof pea roots. An artificially induced strand, once it is formed,attracts towards itself newly induced vascular strands, andthis attraction results in the union of old and new strands.It is also shown that the application of auxin to natural vasculartissues prevents their being joined by newly induced vascularstrands. It is proved that this is dependent on a directionaleffect and not simply on a local accumulation of auxin. To understand these results, it must be assumed that the polarityin terms of auxin transport is increased during the processof vascular tissue induction. The same polarity, once established,is maintained by the presence of auxin, so that the differentiationof strands perpendicular to the axis of this polarity is prevented.These characteristics of plant tissues concerning auxin transportexplain the basic phenomena of the organization of vascularcells in defined and ordered strands. 相似文献
4.
Vascular continuity and auxin signals 总被引:8,自引:0,他引:8
Plant vascular tissues form systems of interconnected cell files throughout the plant body. Vascular tissues usually differentiate at predictable positions but the wide range of functional patterns generated in response to abnormal growth conditions or wounding reveals partially self-organizing patterning mechanisms. Signals ensuring aligned cell differentiation within vascular strands are crucial in self-organized vascular patterning, and the apical-basal flow of indole acetic acid has been suspected to act as an orienting signal in this process. Several recent advances appear to converge on a more precise definition of the role of auxin flow in vascular tissue patterning. 相似文献
5.
Responses of plant vascular systems to auxin transport inhibition. 总被引:28,自引:0,他引:28
To assess the role of auxin flows in plant vascular patterning, the development of vascular systems under conditions of inhibited auxin transport was analyzed. In Arabidopsis, nearly identical responses evoked by three auxin transport inhibitor substances revealed an enormous plasticity of the vascular pattern and suggest an involvement of auxin flows in determining the sites of vascular differentiation and in promoting vascular tissue continuity. Organs formed under conditions of reduced auxin transport contained increased numbers of vascular strands and cells within those strands were improperly aligned. In leaves, vascular tissues became progressively confined towards the leaf margin as the concentration of auxin transport inhibitor was increased, suggesting that the leaf vascular system depends on inductive signals from the margin of the leaf. Staged application of auxin transport inhibitor demonstrated that primary, secondary and tertiary veins became unresponsive to further modulations of auxin transport at successive stages of early leaf development. Correlation of these stages to anatomical features in early leaf primordia indicated that the pattern of primary and secondary strands becomes fixed at the onset of lamina expansion. Similar alterations in the leaf vascular responses of alyssum, snapdragon and tobacco plants suggest common functions of auxin flows in vascular patterning in dicots, while two types of vascular pattern alterations in Arabidopsis auxin transport mutants suggest that at least two distinct primary defects can result in impaired auxin flow. We discuss these observations with regard to the relative contributions of auxin transport, auxin sensitivity and the cellular organisation of the developing organ on the vascular pattern. 相似文献
6.
7.
Integrating cellular and organismic aspects of vascular differentiation 总被引:15,自引:0,他引:15
Sachs T 《Plant & cell physiology》2000,41(6):649-656
Vascular differentiation can be studied at two levels, and they should complement one another: as an aspect of integrated plant development and as cellular processes. The differentiation of organized strands that connect between organs is induced by polar auxin flow, towards the roots. Anatomy, therefore, can be a complementary method of observing polarity and its changes. As expected for a self-correcting and essential system, vascular patterning mutations are relatively rare and have pleiotropic effects, including modifications of responses to auxin and its transport. Tissue polarity both expresses and depends on auxin transport, a feedback that could account for the determined nature of polarity as well as the gradual canalization of differentiation to vascular strands. This predicts that the molecules responsible for polarity will be localized gradually as differentiation proceeds. Further, a modified location of these molecules can be expected to precede anatomical expressions of a new, regenerated, polarity. Tracheary differentiation is probably the best studied example of cell differentiation. Within the plant, however, this differentiation is coupled to oriented cell growth either along or at right angles to the axis of auxin flow, depending on tissue competence. Differentiation is also coupled to the differentiation of the other components of the vascular system. There are, presumably, early joint stages to these differentiation processes, but what they are remains an intriguing problem. 相似文献
8.
Plasmodesma-mediated selective protein traffic between "symplasmically isolated" cells probed by a viral movement protein 总被引:1,自引:0,他引:1
下载免费PDF全文
![点击此处可从《The Plant cell》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Intercellular communication is essential for differentiation and development. In plants, plasmodesmata (PD) form cytoplasmic channels for direct communication. During plant development, programmed reduction in PD number and transport capacity creates the so-called symplasmic domains. Small fluorescent dyes and ions can diffuse among cells within a domain but not across domain boundaries. Such symplasmic isolation is thought to allow groups of cells to differentiate and develop into tissues with distinct structures and functions. Whether or how "symplasmically isolated" cells communicate with one another is poorly understood. One well-documented symplasmic domain is the sieve element-companion cell (SE-CC) complex in the phloem tissue. We report here that, when produced in the CC of transgenic tobacco, the 3a movement protein (3a MP) of Cucumber mosaic virus fused to green fluorescent protein (GFP) can traffic out of the SE-CC complex via PD. The extent of 3a MP:GFP traffic across the boundary between vascular and nonvascular tissues depends on organ type and developmental stage. Our findings provide experimental evidence that endogenous machinery exists for protein traffic between the symplasmically isolated SE-CC complex and neighboring cells. We suggest that PD-mediated traffic of selected macromolecules can be a mechanism for symplasmically isolated cells to communicate with one another. 相似文献
9.
1H magnetic resonance microtomography imaging was applied to study vascular systems in developing bean (Phaseolus limensis L.) seeds. Using the gradient echo method, we recorded 2D tomographic sections in the sagittal and axial planes of the fruits
sampled from a vegetating plant on days 10, 17, 24, and 31 after fertilization. Any vascular connection between the tissues
of maternal plant (bean pod and seed coat) and the embryo were undetectable. The embryo has an autonomous branched network
of procambial strands in the cotyledons, converging to the embryonic axis. The bean pods are covered with a network of vascular
bundles; large vascular strands run along the dorsal and ventral sutures. The seed coat vascular bundles are formed in the
process of seed ripening and are represented by a developed vascular system multiply branching in the middle part of the ground
parenchyma at the stage of physiological maturity. They are connected with the source of assimilates via the lateral pod veins
and a large vascular bundle, entering the seed below the hilum via the placenta. Assimilates enter the external part of the
seed coat, which contains no vascular bundles, via the funiculus vascular bundles and hilum tissue. 相似文献
10.
The temporal and spatial patterns of storage-globulin mobilization were immunohistochemically pursued in the embryonic axis
and cotyledons of vetch seed (Vicia sativa L.) during germination and early seedling growth. Embryonic axes as well as cotyledons of mature seeds contain protein bodies
with stored globulins. Prevascular strands of axes and cotyledons, the radicle and epidermal layers of axis organs were nearly
exclusively stained by vicilin antibodies whereas the cotyledonous storage mesophyll gave similar staining for vicilin and
legumin. Globulin breakdown started locally where growth and differentiation commenced in the axis. There, vicilin mobilization
preceded legumin mobilization. Thus vicilin represents the initial source of amino acids for early growth and differentiation
processes in vetch. Legumin presumably only serves as a bulk amino acid source for subsequent seedling growth during postgerminative
globulin degradation. During the first 2–3 d after the start of imbibition the axis was depleted of globulins whereas no decrease
in immunostainability was detected in the cotyledons except in their vascular strands where immunostainability was almost
completely lost at this time. Continuous vascular strands were established at the third day when globulin breakdown was finished
in the axis but had just started in the cotyledon mesophyll. Protein mobilization proceeded in a small zone from the epidermis
towards the vascular strands in the center of the cotyledons. In this zone the storage cells, which initially appeared densely
packed with starch grains and protein bodies, concomitantly transformed into cells with a large central vacuole and only a
thin cytoplasmic layer attached to the cell wall. These results agree well with the hypothesis that during the first 2 d after
imbibition the axis is autonomous in amino acid provision. After the endogenous reserves of the axis are depleted and the
conductive tissue has differentiated, globulins are mobilized in the cotyledons, suggesting that then the amino acid supply
is taken over by the cotyledons. For comparison with other degradation patterns we used garden bean (Phaseolus vulgaris L) and rape (Brassica napus L.) as reference plants.
Received: 3 August 1999 / Accepted: 11 December 1999 相似文献
11.
旱生植物驼绒藜茎的异常次生结构及其发育 总被引:12,自引:2,他引:12
藜科旱生植物驼绒藜(Ceratoides latens)成长茎由木栓组织、异常形成层、不规则排列的异常维管束及其间的木质化厚壁结合组织和中央正常的维管柱所组成。木栓组织很发达。异常形成层连成环状。异常维管束外韧型。结合组织细胞紧密围绕在各个异常维管束之间。正常的次生维管柱由数个扇形维管束组成,位于维管束内的束中形成层已失去分生能力。中央的髓细胞大多破毁。在茎的发育过程中,初生生长和早期的次生生长都是正常的。而以后的次生生长由初生韧皮部外方保留的原形成层细胞发生的异常形成层活动所代替。起初,异常形成层仅向内交替产生结合组织细胞和异常维管束,而后来同时还向外分化木栓细胞。这种异常的次生结构对旱生植物具有重要的生态学意义。 相似文献
12.
1H magnetic resonance microtomography imaging was applied to study vascular systems in developing bean (Phaseolus limensis L.) seeds. Using the gradient echo method, we recorded 2D tomographic sections in the sagittal and axial planes of the fruits sampled from a vegetating plant on days 10, 17, 24, and 31 after fertilization. Any vascular connection between the tissues of maternal plant (bean pod and seed coat) and the embryo were undetectable. The embryo has an autonomous branched network of procambial strands in the cotyledons, converging to the embryonic axis. The bean pods are covered with a network of vascular bundles; large vascular strands run along the dorsal and ventral sutures. The seed coat vascular bundles are formed in the process of seed ripening and are represented by a developed vascular system multiply branching in the middle part of the ground parenchyma at the stage of physiological maturity. They are connected with the source of assimilates via the lateral pod veins and a large vascular bundle, entering the seed below the hilum via the placenta. Assimilates enter the external part of the seed coat, which contains no vascular bundles, via the funiculus vascular bundles and hilum tissue. 相似文献
13.
Zizhang Wang Yongli Xie Haiwen Zhang Jianzhao Lin Qiang Liu Tai Wang 《Plant Growth Regulation》2010,61(1):85-95
Unlike the ordered multiplication of vascular cells deriving from a row of initials in dicotyledons, vascular growth in monocotyledonous
vascular strands does not show the procambial pattern but leads to a complex organization of the vascular bundle. Establishment
of the bundle should have a specific developmental pattern. The cell cycle conferring cell proliferation represents a active
state of growth and development of tissues. Here, we cloned an A-type CDK gene (Sacof;CDKA;1) from sugarcane (Saccharum officinarum cv. ROC16) and confirmed that its encoding protein interacted physically with two sugarcane CYCD4s (Sacof;CYCD4;1 and Sacof;CYCD4;2),
which shared only 47% amino acid sequence similarity. The three genes were expressed concurrently in meristems of root tip,
stem tip, and young leaf but not in mature leaves. More importantly, they were predominantly expressed in vascular strands
of stem tips and young leaves. In stem-tip strands, the expression region extends deep basipetally to where the sieve tube
increases in number in the metaphloem and the vessels are produced in the metaxylem showing a pattern of cell division occurring
among differentiating or differentiated cells. This pattern suggests a positional determination of vascular cell arrangement
in strands during vascular development. 相似文献
14.
15.
Agrobacterium 6b oncogenes induce tumours and modify plant growth in various ways. Here we show that the AB-6b gene from strain AB4 placed under 2x35S promoter control (2x35S-AB-6b) induces a complex enation syndrome in transgenic Nicotiana tabacum plants, that also occurs in a few rare cases of genetic enations. In Arabidopsis thaliana, 2x35S-AB-6b induced radially symmetrical tubes on the abaxial side of the leaves, which must therefore be considered as the Arabidopsis equivalents of enations on other plant species. Tobacco and Arabidopsis 2x35S-AB-6b leaves contained small, supernumerary densely packed cells between the spongy mesophyll and the abaxial epidermis, close to vascular strands arising at an early stage of leaf development. On tobacco, the 2x35S-AB-6b enation syndrome could be transmitted across graft junctions to growing tissues of untransformed plants, both acropetally and basipetally. We propose that the AB-6b gene encodes the synthesis of one or more enation factor(s) that are transported by the phloem and modify the growth of developing tissues. 相似文献
16.
Plant vascular cells are joined end to end along uninterrupted lines to connect shoot organs with roots; vascular strands are thus polar, continuous, and internally aligned. What controls the formation of vascular strands with these properties? The “auxin canalization hypothesis”—based on positive feedback between auxin flow through a cell and the cell's capacity for auxin transport—predicts the selection of continuous files of cells that transport auxin polarly, thus accounting for the polarity and continuity of vascular strands. By contrast, polar, continuous auxin transport—though required—is insufficient to promote internal alignment of vascular strands, implicating additional factors. The auxin canalization hypothesis was derived from the response of mature tissue to auxin application but is consistent with molecular and cellular events in embryo axis formation and shoot organ development. Objections to the hypothesis have been raised based on vascular organizations in callus tissue and shoot organs but seem unsupported by available evidence. Other objections call instead for further research; yet the inductive and orienting influence of auxin on continuous vascular differentiation remains unique. 相似文献
17.
18.
Atabekov JG Malyshenko SI Morozov YuS Taliansky ME Solovyev AG Agranovsky AA Shapka NA 《Philosophical transactions of the Royal Society of London. Series B, Biological sciences》1999,354(1383):629-635
The phenomenon of trans-complementation of cell-to-cell movement between plant positive-strand RNA viruses is discussed with an emphasis on tobamoviruses. Attention is focused on complementation between tobamoviruses (coding for a single movement protein, MP) and two groups of viruses that contain the triple block of MP genes and require four (potato virus X) or three (barley stripe mosaic virus) proteins for cell-to-cell movement. The highlights of complementation data obtained by different experimental approaches are given, including (i) double infections with movement-deficient (dependent) and helper viruses; (ii) infections with recombinant viral genomes bearing a heterologous MP gene; (iii) complementation of a movement-deficient virus in transgenic plants expressing the MP of a helper virus; and (iv) co-bombardment of plant tissues with the cDNAs of a movement-dependent virus genome and the MP gene of a helper virus. 相似文献
19.
Signaling triggered by polypeptide growth factors leads to the activation of their target genes. Several homeobox genes are known to be induced in response to polypeptide growth factors in early Xenopus development. In particular, Xmsx-1, an amphibian homologue of vertebrate Msx-1, is well characterized as a target gene of bone morphogenetic protein (BMP). Here, using a dominant-negative form of Xmsx-1 (VP-Xmsx-1), which is a fusion protein made with the virus-derived VP16 activation domain, we have examined whether Xmsx-1 activity is required in the endogenous ventralizing pathway. VP-Xmsx-1 induced a secondary body axis, complete with muscle and neural tissues, when overexpressed in ventral blastomeres, suggesting that Xmsx-1 activity is necessary for both mesoderm and ectoderm to be ventralized. We have also examined the epistatic relationship between Xmsx-1 and another ventralizing homeobox protein, Xvent-1, and show that Xmsx-1 is likely to be acting upstream of Xvent-1. We propose that Xmsx-1 is required in the BMP-stimulated ventralization pathway that involves the downstream activation of Xvent-1. 相似文献