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1.
Understanding the landscape factors governing population connectivity in riverine ecosystems represents an ongoing challenge for freshwater biologists. We used DNA sequence analysis to test the hypothesis that major geomorphological features underpin freshwater-limited fish diversity in a tectonically dynamic region of New Zealand. Phylogeographic analysis of 101 Galaxias depressiceps cytochrome b sequences, incorporating 55 localities from southern New Zealand, revealed 26 haplotypes, with only one shared among rivers. We detect strong hierarchical genetic differentiation both among and within river systems. Genetic structuring is particularly pronounced across the Taieri River system (63 individuals from 35 sites, 18 haplotypes), with 92% of variation partitioned among locations. Distinctive within-river genetic clusters are invariably associated with major subcatchment units, typically isolated by substantial gorges. The anomalous distribution of a single lineage across a major drainage divide is consistent with local, tectonically driven headwater capture. We conclude that major landscape features such as gorges can strongly partition riverine fish diversity and constrain freshwater biodiversity. 相似文献
2.
We examined genetic differentiation among 23 samples of non-migratory river galaxias from 17 streams in the Taieri River system, South Island, New Zealand. Four major genetic types were found, two of which occur in narrow sympatry in one location. These were compared with topotypical material representing Galaxias anomalus from the Clutha system (Otago) and G. vulgaris from the Waimakariri system (Canterbury) in order to establish identity. Morphological examination of these four major genetic types revealed consistent concomitant differences. The results suggest that there are at least three species of river galaxias in the Taieri system: G. anomalus, G. vulgaris and at least one previously undescribed species. We propose that the genetic structuring and subsequent speciation of this group has been promoted by the absence of the marine juvenile phase that is found in five other members of the genus native to New Zealand. This structuring may be exacerbated by population fragmentation over the last century owing to the negative influence of introduced trout. The phylogenetic diversity within the river system mirrors the diverse flora and invertebrate fauna of the region, and has conservation implications that parallel those resulting from our improved knowledge of the New Zealand herpetofauna through the application of genetic analysis. 相似文献
3.
Allelic frequencies at five polymorphic loci were determined in seven European and six Afrotropical populations of Drosophila melanogaster. African populations, which may be considered as ancestral for the species, showed a greater genetic diversity as measured by the number of alleles found. Within each geographic group (Europe or tropical Africa) genetic distances between local populations were very small ( D=0.027). By contrast, the average distance between European and African populations ( D=0.389) was more than 12 times bigger. It was previously known that various morphological or physiological differences, which probably reflect genetic adaptations to different environments, exist between these temperate and tropical populations. Data presented here suggest that the divergence in allozyme frequencies also reflects some selective mechanisms. 相似文献
4.
Samples of the spotted murrel (Channa punctatus) were collected from three rivers of Tamil Nadu and Kerala. The allozyme variation of C. punctatus was investigated by polyacrylamide gel electrophoresis. Eighteen enzymes were detected, but only 10 (EST, PGM, G3PDH, G6PDH,
SOD, GPI, ODH, GDH, XDH, and CK) showed consistent phenotypic variations. Allele frequencies were estimated at the 18 polymorphic
loci representing 10 enzymes. Two rare alleles, EST-4*C and G6PDH-2*C, were noted in the Tamirabarani and Kallada populations
but were absent in the Siruvani population. The allele frequencies of the Tamirabarani and Kallada populations were similar,
except for a few loci. Among the three populations, the maximum genetic distance (0.026) and FST (0.203) were found between the geographically distant Siruvani and Kallada populations. Overall the study showed that among
the three populations, the Tamirabarani and Kallada have similar genetic structures. 相似文献
5.
Genetic variation and differentiation of 12 populations of Picea jezoensis from the Russian Far East were studied using 20 allozyme loci. The mean number of alleles per locus was 2.63, the percent
of polymorphic loci was 88.1%, the observed heterozygosity was 0.181, and the mean value of expected heterozygosity amounted
to 0.189. The values of expected heterozygosity of the northern and central mainland populations were higher than in the southern
part of the natural range. A significant bias of Hardy–Weinberg heterozygosity to equilibrium heterozygosity (H eq) suggests that most of the mainland populations have recently experienced a severe expansion in population size while populations
from Kamchatka Peninsula have undergone a reduction in population size. Unbiased Nei’s genetic distance values were low within
and between the mainland and Sakhalin Island populations (D N=0.008). The largest values (D N=0.063) were found between the mainland/Sakhalin and Kamchatka Peninsula populations. Based on genetic distance, P. jezoensis and P. kamtschatkensis could be considered as distinct taxa, but P. ajanensis, P. microsperma, and P. komarovii do not warrant taxonomic recognition. 相似文献
6.
Liatris helleri (Asteraceae) is an insect-pollinated herbaceous perennial endemic to several high-elevation sites in the Blue Ridge Mountains of North Carolina. Allozymes were used to describe the genetic diversity and population structure in nine populations of this rare, federally listed species. Differences in leaf morphology were also examined for greenhouse-grown plants representing several populations. The proportion of the total genetic diversity found among populations, as indicated by the allozyme data, was 16%. Higher levels of population differentiation were found for differences in leaf shape; population of origin accounted for 37% of the variation in maximum leaf width, while families within populations accounted for 7%. In contrast to many endemic species, L. helleri maintains fairly high levels of genetic diversity. For the species, the percent polymorphic loci was 87.5, the average number of alleles at variable loci was 3.00 and the gene diversity was 0.276. Mean population values were percent polymorphic loci =58.4, mean number of alleles per polymorphic locus =2.59 and gene diversity =0.219. The estimated gene flow was low ( Nm=0.85–1.32) and a relatively high correlation ( r=0.55; p<0.005) was found between linear geographic and genetic distance. This suggests that the populations are partially isolated by distance, despite the limited range (<60 km) of the species. We recommend that population distinetiveness be maintained in restoration efforts. 相似文献
7.
Gossypium arboreum L. and G. herbaceum L. are the diploid species of cultivated cotton. Little is known regarding the time and place of domestication of either species. Because G. arboreum is known only as a cultigen, others have proposed that it arose from domesticated G. herbaceum during the more than 5,000-year history of Old World cotton cultivation, with wild G. herbaceum subsp. africanum (Watt) Mauer as the putative ancestor of both species. An alternative hypothesis is that the two species have independent origins from progenitors that diverged prior to domestication. The relative merits of these opposing hypotheses were evaluated using data derived from starch gel electrophoresis of enzymes. One hundred and three accessions of G. arboreum and 31 accessions of G. herbaceum were examined for allelic variation at 40 allozyme loci. All measures of genetic variability demonstrated that G. arboreum contains greater diversity than G. herbaceum, although both species have relatively low levels of allozyme variation. In contrast to expectations based on morphology and other chemical data sets, the two species are highly differentiated with respect to allozyme composition. Gossypium arboreum and G. herbaceum each contain a large number of unique alleles and are fixed or nearly fixed for alternate alleles at 8 loci. Five allozyme loci have alleles that are rare in one of the two species but common in the other. Based on restricted occurrence of these alleles to areas with a long history of sympatric cultivation and the geographic distribution of a null allele, we suggest that a significant portion of the allelic diversity in both species results from historical, bidirectional interspecific introgression. The interspecific genetic identity estimate (0.74) is markedly lower than for documented progenitor-derivative and crop-ancestor species pairs. Based on these data, as well as previous cytogenetic data and the observation of F 2 breakdown in interspecific crosses, we suggest that cultivated G. arboreum and G. herbaceum were independently domesticated from divergent ancestors. 相似文献
8.
Three species of hoplonemerteans, all very similar in external features ( Oerstedia oculata, Oerstedia phoresiae, and Oerstedia zebra) were compared using 23 allozyme loci. The results of this study confirm the specific validity of O. phoresiae. At 20 of the 23 loci this species shares no common alleles with O. oculata ( D = 2.718) and at 21 of the 23 loci O. phoresiae shares no common alleles with O. zebra ( D = 2.730). Most likely O. zebra is a color variation of O. oculata, because these species are very similar in their allozyme markers ( D = 0.000). 相似文献
9.
Several European species of the terrestrial slug genus Arion have been introduced into North America. A case in point is the species complex A. subfuscus s.l. which has become one of the most abundant slug taxa in North America. In Europe this complex consists of at least two
cryptic species, viz. A. fuscus and A. subfuscus s.s., the latter of which is further subdivided in five strongly divergent mtDNA lineages ( A. subfuscus S1–S5). In order to determine which of these A. subfsucus s.l. taxa are present in the NE USA and in order to assess their population genetic structure, we compared mtDNA, nDNA and
allozyme variation between populations from the NE USA and Europe. Our results show that (1) at least A. subfuscus S1 has become successfully established in the NE USA, (2) founder effects are the most likely explanation for the loss of
a large amount of molecular genetic variation in populations from the NE USA (i.e. a loss of 96% of the 16S rDNA haplotypes,
67% of the ITS1 alleles and 46% of the alleles at polymorphic allozyme loci), and (3) part of the remaining genetic variation
in NE USA populations was probably due to multiple introductions from the British Isles and the European mainland, and the
hybrid structure of most of these source populations. Apparently, the extreme loss of molecular genetic variation in this
introduced species has not prevented it from successfully establishing and spreading in novel environments. 相似文献
10.
Hypostomus hermanni (Ihering, 1905) and three other morphotypes of the genus Hypostomus were collected from the Ribeirã;o Keller, a small tributary of Rio Ivaí, a tributary of upper Rio Paraná. An allozyme analysis of 25 gene loci revealed diagnostic loci alleles for each population and there were fixed differences between some of them at some loci. Thus all populations were genetically distinct, although there were many common alleles. Heterozygosity ( H e) ranged from 0.059 in Hypostomus sp. 2 to 0.144 in Hypostomus sp. 1 and was higher than the average for other species of Hypostomus and also for fish in general. H. hermanni and Hypostomus sp. 1 were more similar to each other ( I= 0.878) whereas H. hermanni and Hypostomus sp. 2 showed the least genetic identity ( I= 0.392). 相似文献
11.
Different species of the bristletail genus Lepismachilis were collected in 14 localities in Italy and Spain and an allozyme electrophoretic survey was carried out to estimate the degree of genetic variability and differentiation at intra- and interspecific levels. Four morphological species were initially identified ( L osellai, L. y-signata, L. affinis, L. targionii), but the electrophoretic analysis demonstrated the presence of two additional species among the individuals of L. targionii ( Lepismachilis spl and sp2). The validity of these species and their differentiation from L targionii were demonstrated by the fixation of alternative allelic patterns at several loci (7 in Lepismachilis spl and 8 in Lepismachilis sp2), coupled with fixed, previously undetected, morphological differences. In addition, Lepismachilis sp2 was sympatric with L. targionii in three collecting sites, where the fixation of alternative allelic patterns unequivocally demonstrated reproductive isolation. Genetic variability did not seem to be correlated with local ecological factors, and differences between species should rather be explained by different historical factors. Low levels of gene flow, estimated with two different indirect methods, were observed in L. targionii and L. y-signata, and were due to high levels of structuring among populations. Genetic differentiation among conspecific populations was not correlated to their geographical arrangement and the presence of loci fixed for different alleles among them suggested that stochastic factors (such as genetic drift) may have played a role in determining genetic differentiation of geographically isolated populations. Genetic divergence values indicated that the six species are well differentiated and allozyme profiles were diagnostic for all of them. On the other hand, allozyme data did not provide adequate information to resolve evolutionary relationships among the species, nor did they confirm the validity of the two subgenera ( Lepismachilis and Berlesilis) in which the genus Lepismachilis is traditionally divided. 相似文献
12.
Population genetic affinities of 261 European sculpins Cottus gobio L. across the Rhenanian-Danubian and the Rhenanian-Rhónian watersheds were assessed by horizontal agarose-gel electrophoresis of up to 20 allozyme systems (encoded by 29 genetic loci). Polymorphism P mean= 0.0689 (range: 0.000-0.1379), and heterozygosity H e(mean)= 0.0167 (range: 0.000-0.0507) indicated low genetic variability within local stocks from single streams. Significant genetic distances D mean= 0.1917 ± 0.0336 (D max= 0.2407), based on differential fixation at 3–6 loci ( Acp-1**, Pgdh**, Fh**, Est-1**, Gpi-2**, and Pgm-1**) distinguished populations from the Neckar catchment basin from those of the Hochhrein-Oberrhein and Danube basins. Differential fixation of alleles and pronounced genetic distances also separated sculpins of the Rh6nian tributary Doubs from Neckarian populations (Dmen= 0.2131 ± 0.0033; Ah**, Acp-1**, Fh**), of the Doubs from the Danube (Dmean= 0.2177 ± 0.0028; Gpi-2**, Pgm-1**, Pgdh**, Ah**), and of the Doubs from the Hochrhein-Oberrhein (D mean= 0.1780; Pgm-1**, Pgdh**, Ah**). Genetic distances between streams within these drainages proved low (Neckar: D men= 0.0047 ± 0.0014, Danube: D mean= 0.026 ± 0.0179, Rhine: D mean= 0.0308). Screening of another 16 small-size samples consisting of 55 sculpins for nine diagnostic loci ( Aat-2**, Acp-1**, Acp-2**, Ah**, Est-1**, Fh**, Gpi-2**, Pgdh** and Pgm-1**) confirmed the genetic homogeneity of sculpins within the Danubian and Neckarian drainage systems, but Neckarian sculpins were similar to those from the river Main. Populations from Hochrhein-Oberrhein resembled the Danubian stock but contained a decreasing frequency of ‘Neckarian’ markers when approaching the Danubian region. The genetic divergence between Neckarian, Danubian and Rhdnian sculpins suggests the existence of hitherto neglected taxa of anteglacial divergence. 相似文献
13.
Genetic differentiation based on allozyme data was detected between species of the genus Astragalus L., section Cenantrum Bunge (Fabaceae): A. frigidus (L.) A. Gray s. l., A. mongholicus Bunge s. 1., and A. sericeocanus Gontsch, which is endemic to the northeast coast of Lake Baikal. The results of allozyme analysis confirm the natural division of the section into subsections based on morphological features. Differences between A. frigidus (subsection Elliptica Gontsch.) and A. mongholicus and A. sericeocanus (subsection Semilunaria Gontsch.) were observed. These differences were caused by the presence of species-specific alleles and the allele frequencies of the primary alleles, D
N = 1.24. The genetic distance obtained for A. mongholicus and A. sericeocanus (0.10) corresponds to the status of closely related species of one subsection. Between populations of A. frigidus and between populations of A. mongholicus from the central part of areal, D
N = 0.02 and 0.03 respectively. This genetic distance corresponds to the interpopulation level and was determined by the allele frequencies. The peripheral population of A. mongholicus is separated (D
N = 0.36), which is probably due to the long isolation and the “founder effect.” 相似文献
14.
Summary Data for restriction fragment length polymorphisms (RFLPs) of 144 clone-enzyme combinations and for 22 allozyme loci from 21 U.S. Corn Belt maize ( Zea mays L.) inbreds were analyzed. The genetic materials included 14 progenitors of the Iowa Stiff Stalk Synthetic (BSSS) maize population, both parents of one missing BSSS progenitor, four elite inbreds derived from BSSS, and inbred Mo17. Objectives were to characterize the genetic variation among these 21 inbreds for both allozymes and RFLPs, to compare the results from both types of molecular markers, and to estimate the proportion of unique alleles in the BSSS progenitors. Genetic diversity among the 21 inbreds was substantially greater for RFLPs than for allozymes, but the percentages of unique RFLP variants (27%) and unique allozyme alleles (25%) in the BSSS progenitors were similar. Genetic distances between inbreds, estimated as Rogers' distance (RD), were, on average, twice as large for RFLP (0.51) as for allozyme data (0.24). RDs obtained from allozyme and RFLP data for individual line combinations were only poorly correlated ( r = 0.23); possible reasons for discrepancies are discussed. Principal component analysis of RFLP data, in contrast to allozyme data, resulted in separate groupings of the ten BSSS progenitors derived from the Reid Yellow Dent population, the four BSSS elite lines, and Mo17. The remaining six BSSS progenitors were genetically rather diverse and contributed a large number of rare alleles to BSSS. The results of this study corroborate the fact that RFLPs are superior to allozymes for characterizing the genetic diversity of maize breeding materials, because of (1) the almost unlimited number of markers available and (2) the greater amount of polymorphisms found. In particular, RFLPs allow related lines and inbreds with common genetic background to be identified, but a large number of probe-enzyme combinations is needed to estimate genetic distances with the precision required.Joint contribution from Cereal and Soybean Research Unit, USDA, Agricultural Research Service, and Journal Paper No. J-14236 of the Iowa Agricultural and Home Economics Experiment Station, Projects 2818 and 2778 相似文献
15.
Drosophila antonietae is an endemic South American cactophilic species that uses Cereus hildmaniannus rotting cladodes as breeding sites. We assessed temporal and spatial intrapopulational allozyme variation of two natural populations. Our results suggest that environmental variation (rain precipitation) is probably influencing allozyme temporal variation. Moreover, it seems that D. antonietae does not have intrapopulation structure and has N
ev (variance effective size) 83 and N
ec (number of adult flies that colonize each rotting cladode) = 21. The deficiency of heterozygotes found must be due to null alleles, a temporal Wahlund effect, or selection against heterozygotes. Assortative mating and inbreeding are discarded. This is the first report on allozyme variation in D. antonietae. It gives some insight on intrapopulational genetics through space and time for this species. This is important to understand its general genetic variability and will be essential to future works on the natural history and evolution of this species. 相似文献
16.
We examined genetic variation in allozyme loci, nuclear DNA restriction fragment length polymorphisms (RFLPs), and random amplified polymorphic DNAs (RAPDs) in 130 trembling aspen ( Populus tremuloides) and 105 bigtooth aspen ( P. grandidentata) trees. In trembling aspen 10 out of 13 allozyme loci assayed (77%) were polymorphic (P), with 2.8 alleles per locus (A) and an expected heterozygosity (H e) of 0.25. In contrast, bigtooth aspen had a much lower allozyme genetic variability (P=29%; A=1.4; H e=0.08). The two species could be distinguished by mutually exclusive alleles at Idh-1, and bigtooth aspen has what appears to be a duplicate 6PG locus not present in trembling aspen. We used 138 random aspen genomic probes to reveal RFLPs in HindIII digests of aspen DNA. The majority of the probes were from sequences of low copy number. RFLP results were consistent with those of the allozyme analyses, with trembling aspen displaying higher genetic variation than bigtooth aspen (P=71%, A=2.7, and H e=0.25 for trembling aspen; P=65%, A=1.8, and H e=0.13 for bigtooth aspen). The two species could be distinguished by RFLPs revealed by 21 probes (15% of total probes assayed). RAPD patterns in both species were studied using four arbitrary decamer primers that revealed a total of 61 different amplified DNA fragments in trembling aspen and 56 in bigtooth aspen. Assuming a Hardy-Weinberg equilibrium, estimates of P=100%, A=2, and H e=0.30 in trembling aspen and P=88%, A=1.9, and H e=0.31 in bigtooth aspen were obtained from the RAPD data. Five amplified DNA fragments were species diagnostic. All individuals within both species, except for 2 that likely belong to the same clone, could be distinguished by comparing their RAPD patterns. These results indicate that (1) RFLPs and allozymes reveal comparable patterns of genetic variation in the two species, (2) trembling aspen is more genetically variable than bigtooth aspen at both the allozyme and DNA levels, (3) one can generate more polymorphic and species-specific loci with DNA markers than with allozymes in aspen, and (4) RAPDs provide a very powerful tool for fingerprinting aspen individuals. 相似文献
17.
Natural selection has been invoked to explain the observed geographic distribution of allozyme allele frequencies for a number of teleost species. The effects of selection on allozyme loci in three species of Pacific salmon were tested. A simulation-based approach to estimate the null distribution of population differentiation ( F
ST) and test for F
ST outliers was used. This approach showed that a majority of allozyme loci conform to neutral expectations predicted by the simulation model, with relatively few F
ST outliers found. No consistent F
ST outlier loci were found across species. Analysis of population sub-groups based on geography and genetic identity reduced the number of outlier loci for some species, indicating that large geographic groups may include genetically divergent populations and/or that there is geographic heterogeneity in selection pressure upon allozyme loci. Two outlier allozyme loci found in this analysis, lactate dehydrogenase-B and malic enzyme, have been shown to be influenced by selection in other teleost species. This approach is also useful in identifying allozyme loci (or other genetic markers) that meet assumptions for population genetic study. 相似文献
18.
Patterns of variation at 27 allozyme loci were investigated in the endangered endemic plant Megaleranthis saniculifolia. Levels of allozyme variation ( A = 1.47, P = 40%, He = 0.088) were also compared with other endemic plant species. Genetic divergence between populations was very high ( G
st
= 0.271 ), with moderate to high interpopulation differentiation, which probably arose through historical bottlenecks in
a landscape of habitat fragmentation and/or human influence. The percentage of polymorphic loci, heterozygosity, and mean
number of alleles per locus were positively related to population size, probably due to the stochastic loss of rare alleles
in the smaller populations. Individuals in the small and marginal populations (TB, KD, and CJ) showed higher proportions of
fixed loci. These ecologically marginal populations were typically more distant from the nearest neighboring population and
were more genetically distinct from one another. The genetic structure of the current population of M. saniculifolia is probably the result of local extinctions of intervening populations. This, in turn, is due to the Pleistocene climatic
change and increased habitat destruction. A positive association appears to exist between genetic diversity and population
size. Although these small population sizes are more sensitive to stochastic events, securing a certain number of individuals
from the three larger populations (SB, JB, and TG) could be accomplished as part of a conservation strategy. In addition,
it is important to prioritize populations in different regions in order to limit population declines caused by large-scale
environmental catastrophes. 相似文献
19.
Phylogenetic relationships between sympatric, morphologically indistinguishable diploid and tetraploid plants of Dactylis glomerata L. ( Gramineae) in Galicia (Spain) were assessed using allozyme markers for 6 distinct systems. The study exploited recent introduction in Galicia and subsequent hybridization of an alien 4x Dactylis subspecies possessing distinct allozymes from those of all the native plants. Opportunities for gene exchanges between the ploidies were estimated from in situ observations of flowering, examination of progenies in 2x/4x natural and experimental crosses, and enzyme analyses. Results show a high genetic similarity between the Galician diploids and tetraploids, which possess peculiar alleles in common. Although the ploidy levels usually have distinct flowering periods, interploidal crosses do occasionally occur. Gene flow is likely much more important from the diploid to the tetraploid level. A good genetic intermixing occurs between the Galician and the alien tetraploid entities which have simultaneous flowering. Autopolyploidization of the diploids followed by various rates of hybridization is proposed as one very probable origin of natural tetraploids in Dactylis. 相似文献
20.
The genetic relatedness between the green and red forms of the two-spotted spider mite, Tetranychus urticae Koch, was analysed using an allozyme marker, MDH, detected by polyacrylamide gel electrophoresis. Cross experiments showed that each of the two forms had form-specific alleles for MDH. Furthermore, there was no overlapping in the alleles for MDH between the field populations of the two forms, suggesting that no gene flow would occur between them in the field. 相似文献
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