Length–weight relationships and biological data on guayana pike‐conger Cynoponticus savanna (Bancroft, 1831)

This study presents the first references available for Cynoponticus savanna length–weight relationships (LWR) as well as other biological information, based on its unique population in the South Atlantic Ocean, specifically, Todos os Santos Bay, on the Brazilian central coast. A total of 464 individuals were obtained from artisanal fishing landings between July 2012 and June 2013. Total lengths ranged from 14.5 to 139.6 cm. The length‐weight relationship was BW = 0.716 TL^3.31. Length at first maturity (L_m) was 48.9 cm. Reproduction began gradually in May, reaching a peak around December, with most individuals completing reproduction by January. The gonadosomatic index (GSI) was highest in January (GSI = 0.21) and lowest in March (GSI = 0.024). The male index (GSI = 0.073) was lower than in females (GSI = 0.10) (P < 0.01). This study presents the first population data for C. savanna, which is under threat due to overfishing and habitat loss.     

Reproductive biology of Chinese minnow Hemiculterella sauvagei Warpachowski, 1888 in the Chishui River,China

This study describes the reproductive biology of the Chinese minnow Hemiculterella sauvagei. The length‐weight relationship, sex ratio, spawning season, size at first maturity, and fecundity were analyzed based on 685 specimens collected from the Chishui River between July 2011 and July 2012. The relationship between standard length (SL) and body weight (BW) were estimated as BW = 2.14 × 10^?4 × SL^2.801 (R² = 0.839; N = 413; P < 0.05) for females and BW = 1.31 × 10^?4 × SL^3.001 (R² = 0.868; N = 272; P < 0.05) for males. The female to male sex ratio, 1.52 : 1, differed significantly from a 1 : 1 ratio. Females predominated in standard lengths >12 cm. Analyses of the monthly variation in the gonadosomatic index (GSI), the monthly proportions of macroscopic gonadal maturity, and the size distribution of oocytes consistently suggested a prolonged spawning season of H. sauvagei from March to August, with a peak from April to May. Logistic curves describing the relationship between proportion of maturity (P_r) at each length interval and standard length were estimated as P_r = 1/(1 + e^{26.867–0.306SL}) (R² = 0.999; N = 413; P < 0.05) for females and P_r = 1/(1 + e^{10.522–0.142SL}) (R² = 0.999; N = 272; P < 0.05) for males. Size at first maturity was estimated as 7.4 cm for males and 8.8 cm SL for females. Absolute fecundity varied from 563 to 5052, with a mean of 2413 ± 874 oocytes per ovary. The relative fecundity was estimated to be 41–299, with a mean of 171 ± 55 oocytes per ovary. The present study provides useful information for fishery management and resources conservation.     

Length–weight relationship and condition factor of wild,grow‐out and ‘loose‐shell affected’ giant tiger shrimp,Penaeus monodon (Fabricius, 1798) (Decapoda: Penaeidae)

Length–weight relationships and condition factors of wild, cultured, and cultured loose‐shell affected Penaeus monodon (2,609 specimens total) were studied from March to August 2007. The regression equation for healthy cultured shrimps was log W = ?1.811 + 2.721 log L (r² = 0.71); log W = ?1.444 + 2.485 log L (r² = 0.91) for wild shrimps; and log W = ?1.112 + 2.237 log L (r² = 0.92) for loose‐shell affected shrimps. All shrimps showed negative allometric growth, although ancova indicated significant differences (P < 0.05) among them. This study presents the first known reference dealing with LWRs and condition factors of cultured, wild and loose‐shell affected P. monodon.     

Length–weight and length–length relationships and length at first maturity for freshwater fish species of the Salto Santiago Reservoir,Iguaçu River Basin,Brazil

Length‐weight (LWR) and length‐length (LLR) relationships were estimated for 20 species and lengths at first maturity (L₅₀) for six species of freshwater fishes caught in the Salto Santiago Reservoir, Iguaçu River Basin, Brazil. In nine species significant differences were found in the LWR between sexes. Average b‐value for species with no differences between sexes in LWR was 3.12 (SE = ±0.05). Average b‐value in LLR was 0.823. First references on LWRs and L₅₀ are presented for 13 and four fish species, respectively, as well as the new maximum total lengths for eight species.     

The effects of productivity and seasonality on life history: comparing age at maturity among moose (Alces alces) populations

The productivity hypothesis in respect of an animal species’ geographical range predicts that whereas higher productivity at the equatorial periphery of a species’ range favours superior competitors, lower productivity at the centre of a species’ range favours high reproduction and reduced competitive traits. I test whether life‐history patterns follow this hypothesis, using demographic data from 15 Canadian moose (Alces alces) populations. Two models are contrasted; the first assumes that intraspecific variation in age at maturity is explained proximately by density and juvenile mortality. Age at maturity was found to increase with decreasing juvenile mortality (P = 0.01) and increasing density (P = 0.006). To test the productivity hypothesis, the second model additionally included primary productivity and seasonality as geographical explanatory variables that would ultimately influence age at maturity via juvenile mortality and density. Path analysis indicated that including productivity and seasonality improved the model predictions of variation in age at maturity (R_a² 0.56 and 0.85). In bivariate comparisons, seasonality was negatively associated (P = 0.01) with age at maturity. In the best model, however, primary productivity was the environmental variable that explained 25% of the variance in age at maturity, and forest cover replaced seasonality as an explanatory variable. The positive association between primary productivity and age at maturity is consistent with the productivity hypothesis. Relative to populations that lived at the centre of the species’ range (51°N), moose populations living in relatively high productivity and low seasonality environments (equatorial periphery of species’ range; 48°N) experienced less juvenile mortality, more variable year‐to‐year density, higher relative density and slower life history (slower growth rate, later age at maturity, lower fecundity).     

Age,growth, reproduction and feeding of John Dory,Zeus faber (Pisces: Zeidae), in the Saros Bay (North Aegean Sea)

The age, growth, reproduction, sexual maturity and stomach content of John Dory (Zeus faber), caught in the Bay of Saros (North Aegean Sea) between September 2006 and September 2008, were investigated. The female‐male ratio was 1.6 : 1. The total length of females ranged from 13.8 cm to 52.8 cm, and of males from 12.2 cm to 42.9 cm. The length‐weight relationship was W = 0.0174*L^2.936. Age data derived from vertebral centra readings were used to estimate the growth parameters of the von Bertalanffy equation. Growth parameters for females were L_∞ = 58.50 cm K = 0.11 year^?1, t₀ = ?0.99 year and for males L_∞ = 45.01 cm, K = 0.13 year^?1, t₀ = ?1.17 year. Maximum age was 18 years for females and 17 years for males. Length at first maturity for both females and males was 25.4 cm. Monthly values of the gonadosomatic index indicated that spawning occurred mainly in two peaks: one between January and June, and the other from August to September. Stomach content analysis showed the most‐preferred prey to be Pisces (IRI% 97.3).     

Length‐weight relationships of three cyprinid fishes from headwater of the Nujiang River,China

Length‐weight relationships (LWR) for three cyprinid fish species collected from the headwaters of the Nujiang River in Tibet, China, were determined. The values of parameter b in the LWR equations were estimated as 2.54 for Schizopygopsis thermalis, 3.3 for Ptychobarbus kaznakovi, and 2.74 for Schizothorax nukiangensis, respectively. These are the first LWR records for the three species.     

Genome‐wide association study of osteochondrosis in the tarsocrural joint of Dutch Warmblood horses identifies susceptibility loci on chromosomes 3 and 10

Equine osteochondrosis is a developmental joint disease that is a significant source of morbidity affecting multiple breeds of horse. The genetic variants underlying osteochondrosis susceptibility have not been established. Here, we describe the results of a genome‐wide association study of osteochondrosis using 90 cases and 111 controls from a population of Dutch Warmblood horses. We report putative associations between osteochondrosis and loci on chromosome 3 (BIEC2‐808543; P = 5.03 × 10^?7) and chromosome 10 (BIEC2‐121323; P = 2.62 × 10^?7).     

Diet shift response in round goby,Neogobius melanostomus,based on size,sex, depth,and habitat in the western basin of Lake Erie

This study examines the diet of the round goby (Neogobius melanostomus) in the western basin of Lake Erie. As an invasive benthic feeder, the round goby has the potential to affect smallmouth bass and other native species. Round goby (n = 100) were collected during summer 2011 and stomach contents were examined to determine diet patterns and possible ontogenetic diet shifts. Individual round goby were grouped by sex, size (small < 65 mm, large ≥ 65 mm), depth of habitat (shallow < 2 m, deep > 5 m), and habitat type (natural shallows, anthropogenically modified shallows, and deep waters). Gut fullness ranged from 10 to 100% with 23 stomachs presenting 100% fullness. Round goby consumed 27 different food items including abiotic (sand grains, gravel), floral (algae, fine leaved vascular plants), and fauna items. The Index of Relative Importance (IRI) and comparative z‐tests were used to assess correlations and differences. Two‐way z‐tests revealed a significant difference in mouth gape related to size (z = ?5.56377, P = 2.64e‐08), and habitat depth (z = 3.34262, P = 0.00083). A significant linear correlation was also found between mouth gape standardized by head length (HL) for both males (P = 2.63e‐9) and females (P = 1.3e‐4). Two‐way z‐tests also revealed a significant difference in gonadosomatic index (GSI) related to sex (z = 6.07727, P = 6.11248e‐10), but not size. A significant difference in gut fullness was also found related to sex (z = ?3.34743, P = 0.00082), habitat depth (z = 3.16336, P = 0.00156), and habitat type (z = ?2.7398, P = 0.00615). IRI values demonstrated a diet selective of veliger mussels (IRI = 2462.01), juvenile mussels (IRI = 1073.03), cladocerans (IRI = 4804.31), and chironomids (IRI = 1012.12). While previous studies have focused on round goby diet shifts from macroinvertebrates to bivalves, most studies did not evaluate changes in diet among multiple categories. Furthering knowledge of multiple aspects of goby diet may aid in developing management techniques to deter future round goby invasion.     

Evaluation of the causality of the low‐density lipoprotein receptor gene (LDLR) for serum lipids in pigs

A significant quantitative trait locus (QTL) for low‐density lipoprotein cholesterol (LDL‐C) and total cholesterol (TC) was identified around the LDLR gene on chromosome 2 (SSC2) in a White Duroc × Erhualian F₂ resource population and Sutai pigs in our previous study. However, in previous reports, the causality of LDLR with serum lipids is controversial in pigs. To systematically assess the causality of LDLR with serum lipids, association analyses were successively performed in three populations: Sutai pigs, a White Duroc × Erhualian F₂ resource population and a Duroc × (Landrace × Large White) population. We first performed a haplotype‐based association study with 60K SNP genotyping data and evidenced the significant association with LDL‐C and TC around the LDLR gene region. We also found that there is more than one QTL for LDL‐C and TC on SSC2. Then, we evaluated the causalities of two missense mutations, c.1812C>T and c.1520A>G, with LDL‐C and TC. We revealed that the c.1812C>T SNP showed the strongest association with LDL‐C (P = 5.40 × 10^?11) and TC (P = 3.64 × 10^?8) and explained all the QTL effect in Sutai pigs. Haplotype analysis found that two missense SNPs locate within a 1.93‐Mb haplotype block. One major haplotype showed the strongest significant association with LDL‐C (P = 4.62 × 10^?18) and TC (P = 1.06 × 10^?9). However, the c.1812C>T SNP was not identified in the White Duroc × Erhualian intercross, and the association of c.1520A>G with both LDL‐C and TC did not achieve significance in this F₂ population, suggesting population heterogeneity. Both missense mutations were identified in the Duroc × (Landrace × Large White) population and showed significant associations with LDL‐C and TC. Our data give evidence that the LDLR gene should be a candidate causative gene for LDL‐C and TC in pigs, but heterogeneity exists in different populations.     

Similarities and differences in 13C and 15N stable isotope ratios in two non‐lethal tissue types from shovelnose sturgeon Scaphirhynchus platorynchus (Rafinesque, 1820)

We tested the hypothesis that δ¹³C and δ¹⁵N signatures of pectoral spines would provide measures of δ¹³C and δ¹⁵N similar to those obtained from fin clips for adult shovelnose sturgeon Scaphirhynchus platorynchus. Thirty‐two shovelnose sturgeon (fork length [FL] = 500–724 mm) were sampled from the lower Mississippi River, USA on 23 February 2013. Isotopic relationships between the two tissue types were analyzed using mixed model analysis of covariance. Tissue types differed significantly for both δ¹³C (P < 0.01; spine: mean = ?23.83, SD = 0.62; fin clip: mean = ?25.74, SD = 0.97) and δ¹⁵N (P = 0.01; spine: mean = 17.01, SD = 0.51; fin clip: mean = 17.19, SD = 0.62). Neither FL nor the FL × tissue type interaction had significant (P > 0.05) effects on δ¹³C. Fin clip δ¹³C values were highly variable and weakly correlated (r = 0.16, P = 0.40) with those from pectoral spines. We found a significant FL‐tissue type interaction for δ¹⁵N, reflecting increasing δ¹⁵N with FL for spines and decreasing δ¹⁵N with FL for fin clips. These results indicate that spines are not a substitute for fin clip tissue for measuring δ¹³C and δ¹⁵N for shovelnose sturgeon in the lower Mississippi River, but the two tissues have different turnover rates they may provide complementary information for assessing trophic position at different time scales.     

Dose‐response effects of the antioxidant α‐lipoic acid in the liver and brain of pompano Trachinotus marginatus (Pisces,Carangidae)

This study evaluated the effect of different doses of the antioxidant α‐lipoic acid (LA) administered by intraperitoneal injection on the detoxifying capacity (activity of glutathione‐S‐transferase, GST) and oxidative damage (lipids and proteins) in the pompano, Trachinotus marginatus. The plasma glucose levels showed that there were no differences between the treatments (P > 0.05). In the brain, GST activity was significantly higher (P < 0.05) in fish injected with 40 mg LA kg^?1 when compared with the control group. In the muscle, GST activity was not influenced by LA treatment (P > 0.05). In the liver, fish injected with 20 mg LA kg^?1 showed higher GST activity than the control group (P < 0.05); however, higher doses (40 and 60 mg LA kg^?1) led to a reduction of GST activity in the liver, which was comparable to that observed in the control group (P > 0.05). The two highest LA doses (40 and 60 mg kg^?1) had opposite effects, depending on the tissue examined: LA was an antioxidant in the brain, reducing lipid peroxidation (P < 0.05), and a pro‐oxidant in the liver, augmenting oxidative lipid damage (P < 0.05). The latter effect was accompanied by an increase in the free iron concentration in the liver at higher LA doses. These results indicate the need to thoroughly evaluate the antioxidant effects on aquatic organisms, since at some doses and/or in some organs their beneficial effects can be lost.     

SURVEY FOR KARLOTOXIN PRODUCTION IN 15 SPECIES OF GYMNODINIOID DINOFLAGELLATES (KARENIACEAE,DINOPHYTA)1

Toxin analysis of 15 species of Kareniaceae revealed the presence of karlotoxin, KmTx 2, in only a single species (Karlodinium veneficum) but with variable activity in strains from the Swan (Km^SwanTx 2‐1, 2.1 pg · cell^?1; and Km^SwanTx 2‐2, 0.53 pg · cell^?1), Huon (Km^HuonTx 2, 0.86 pg · cell^?1), and Derwent rivers (<0.001 pg · cell^?1) in Australia. A newly isolated Southern Ocean species, Karlodinium conicum, contained a novel poorly hemolytic karlotoxin analogue (Km_conicumTx, 2.8 pg · cell^?1). The hemolytic potency (HD50%) of the Australian karlotoxins were as follows: Km^SwanTx 2‐1 (65.9 ± 4.8 ng) and Km^SwanTx 2‐2 (63.4 ± 3.7 ng), Km^HuonTx 2 (343 ± 4.9 ng), and Km_conicumTx (>4,000 ng). Species from the closely related genera Takayama (T. helix, T. tasmanica, T. tuberculata), Karenia (K. asterichroma, K. brevis, K. mikimotoi, K. papilionacea, K. umbella), and Karlodinium (Ka. australe, Ka. antarcticum, Ka. ballantinum, Ka. corrugatum, Ka. decipiens) were all consistently negative for karlotoxin production. Brevetoxin (PbTx) was only detected in K. brevis, and hemolytic activity was only observed in Ka. veneficum strains.     

Age‐associated microRNA expression in human peripheral blood is associated with all‐cause mortality and age‐related traits

Recent studies provide evidence of correlations of DNA methylation and expression of protein‐coding genes with human aging. The relations of microRNA expression with age and age‐related clinical outcomes have not been characterized thoroughly. We explored associations of age with whole‐blood microRNA expression in 5221 adults and identified 127 microRNAs that were differentially expressed by age at P < 3.3 × 10^?4 (Bonferroni‐corrected). Most microRNAs were underexpressed in older individuals. Integrative analysis of microRNA and mRNA expression revealed changes in age‐associated mRNA expression possibly driven by age‐associated microRNAs in pathways that involve RNA processing, translation, and immune function. We fitted a linear model to predict ‘microRNA age’ that incorporated expression levels of 80 microRNAs. MicroRNA age correlated modestly with predicted age from DNA methylation (r = 0.3) and mRNA expression (r = 0.2), suggesting that microRNA age may complement mRNA and epigenetic age prediction models. We used the difference between microRNA age and chronological age as a biomarker of accelerated aging (Δage) and found that Δage was associated with all‐cause mortality (hazards ratio 1.1 per year difference, P = 4.2 × 10^?5 adjusted for sex and chronological age). Additionally, Δage was associated with coronary heart disease, hypertension, blood pressure, and glucose levels. In conclusion, we constructed a microRNA age prediction model based on whole‐blood microRNA expression profiling. Age‐associated microRNAs and their targets have potential utility to detect accelerated aging and to predict risks for age‐related diseases.     

The Pillars of Hercules as a bathymetric barrier to gene flow promoting isolation in a global deep‐sea shark (Centroscymnus coelolepis)

Knowledge of the mechanisms limiting connectivity and gene flow in deep‐sea ecosystems is scarce, especially for deep‐sea sharks. The Portuguese dogfish (Centroscymnus coelolepis) is a globally distributed and near threatened deep‐sea shark. C. coelolepis population structure was studied using 11 nuclear microsatellite markers and a 497‐bp fragment from the mtDNA control region. High levels of genetic homogeneity across the Atlantic (Φ_ST = ?0.0091, F_ST = 0.0024, P > 0.05) were found suggesting one large population unit at this basin. The low levels of genetic divergence between Atlantic and Australia (Φ_ST = 0.0744, P < 0.01; F_ST = 0.0015, P > 0.05) further suggested that this species may be able to maintain some degree of genetic connectivity even across ocean basins. In contrast, sharks from the Mediterranean Sea exhibited marked genetic differentiation from all other localities studied (Φ_ST = 0.3808, F_ST = 0.1149, P < 0.001). This finding suggests that the shallow depth of the Strait of Gibraltar acts as a barrier to dispersal and that isolation and genetic drift may have had an important role shaping the Mediterranean shark population over time. Analyses of life history traits allowed the direct comparison among regions providing a complete characterization of this shark's populations. Sharks from the Mediterranean had markedly smaller adult body size and size at maturity compared to Atlantic and Pacific individuals. Together, these results suggest the existence of an isolated and unique population of C. coelolepis inhabiting the Mediterranean that most likely became separated from the Atlantic in the late Pleistocene.     

Age,growth and reproduction of the black sea bream Spondyliosoma cantharus (Linnaeus, ) (Sparidae) in the Gulf of Annaba (Algeria)

The objective of this study was to determine the basic population‐specific parameters necessary for fish stock assessment in the Gulf of Annaba and to compare these with data from other Mediterranean regions. Black sea bream Spondyliosoma cantharus (Linnaeus, 1758) (N = 501) were collected monthly from January to December 2008 along the Algerian eastern coasts. More than 22 fish were collected each month and ranged in size from 13.4 to 40 cm total length, weighing from 36 to 1080 g eviscerated weight. Biological sampling included weighing and measuring the fish, gonad weighing, sex and maturity stage determination, and age estimation through otolith readings. Validity of the otolith readings for estimating age and growth was supported using the back‐calculation method. Estimated parameters of the von Bertalanffy model are: L_∞ = 33.54 cm, W_∞ = 633.46 g, k = 0.52 year^?1 and t_o = ?0.04 year. The growth performance index (φ) is: 2.76. The length‐weight relationship is: EW = 4.4.10^?6 TL^3.23. The spawning period occurred from February to May, while the gamete emission peaked in April. Females reached sexual maturity at 19.3 cm (2 years) and males at 21.3 cm (3 years). Sexual inversion occurs at approximately 24.3 cm. Spondyliosoma cantharus was characterized as being a protogynic hermaphrodite.     

The association between paraoxonase 1 activity and the susceptibilities of diabetes mellitus,diabetic macroangiopathy and diabetic microangiopathy

We carried out this meta‐analysis to explore the influence of paraoxonase 1 activity on the susceptibility of diabetes mellitus (DM), diabetic macroangiopathy and diabetic microangiopathy. Relevant studies were identified from PubMed, Web of Science and CNKI without language limitation, following the inclusion and exclusion criteria. Statistical analyses were implemented with the STATA 12.0 statistical software. Thirty‐six case‐control studies were included in the meta‐analyses, in which 35 for the association between paraoxonase 1 activity and DM risk, 8 for diabetic macroangiopathy and 7 for diabetic microangiopathy. Paraoxonase 1 activity was significantly associated with the susceptibility of DM in pooled population (SMD = ?1.37, 95% CI = ?1.79 ～ ?0.96, P = .000), and Asians (SMD = ?2.00, 95% CI = ?2.56 ～ ?1.44, P = .000), but not in non‐Asians (SMD = ?0.44, 95% CI = ?0.91 ～ 0.03, P = .069). However, marked heterogeneity was existed (I² = 98.10%, P = .000) and subgroup analyses failed to investigate the sources of heterogeneity. Then, meta‐regression was performed and found that ethnicity could explain the observed between‐study heterogeneity (P = .002). Meanwhile, significant associations were found between paraoxonase 1 activity and diabetic macroangiopathy (SMD = ?1.06, 95% CI = ?1.63 ～ ?0.48, P = .000) and diabetic microangiopathy (SMD = ?0.72, 95% CI = ?1.32 ～ ?0.13, P = .018). In conclusion, paraoxonase 1 activity plays important roles in the risk of DM, diabetic macroangiopathy and microangiopathy with ethnicity differences. Further studies with large sample and well design are needed to confirm these results.     

Effects of β‐xylanase and 6‐phytase on digestibility,trace mineral utilisation and growth in juvenile red tilapia,Oreochromis niloticus (Linnaeus, 1758) x O. mossambicus (Peters, 1852), fed declining fishmeal diets

In response to the global sustainability drive to lower fishmeal (FM) inclusion in aquatic feeds, exogenous enzymes can improve nutrient digestibility in monogastric plant‐based diets. A 80‐day experiment was conducted to evaluate the combined effects of xylanase and phytase on digestibility, trace mineral utilisation and growth in juvenile red tilapia, Oreochromis niloticus x O. mossambicus, (48.8 g ± 13.9; μ ± STD) fed declining FM diets. Basal diets were formulated to contain 0, 3 and 5% FM with and without xylanase (0.385 g kg^?1) and phytase (0.075 g kg^?1), forming six treatments. Each treatment was randomly assigned to four replicates, 20 fish tank^?1; mean water temperature 28.98 ± 0.73°C. Although the size of the effects was modest, growth performances (feed intake, FCR, growth rate) decreased with lower FM levels (P < 0.05) but improved with enzyme supplementation. Enzyme supplementation increased P digestibility and trace mineral uptake (P < 0.05), but no effects were seen on protein digestibility and N retention. Nevertheless, tilapia fed the enzyme‐supplemented 3% FM and control 5% FM diets performed comparably (P < 0.05). This potentially justifies a 2% FM reduction for tilapia diets using exogenous xylanase and phytase without significant effects on digestibility, trace mineral utilisation and growth.     

Length–weight relationship of six notothenioid species from sub‐Antarctic waters (Beagle Channel,Argentina)

This study presents length‐weight relationships (LWR) for six species of fishes of the Suborder Notothenioidei that inhabit the sub‐Antarctic waters of the Beagle Channel (Argentina). These species represent five of the nine families of this Suborder. The specimens belong to historical fish collections in the area (from 1987 to 1990) using gill and trammel nets. All species but Eleginops maclovinus had no previous LWR estimates. The coefficient of determination (r²) ranged from 0.91 to 0.99 and b values ranged from 2.74 to 3.2. Harpagifer bispinis was the only species with a b value lower than three. This information is useful to predict the weight from the length data and to provide insights into the ecology of sub‐Antarctic nototheniod fish.