共查询到20条相似文献,搜索用时 15 毫秒
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Eugene A. Berkowitz Marilyn I. Evans 《In vitro cellular & developmental biology. Animal》1992,28(6):391-396
Summary The estrogen-responsive Leghorn strain M chicken hepatoma (LMH) cell line provides a model system for studying the estrogen-dependent,
liver-specific expression of avian genes. Serum-free culture conditions have been established that allow expression of apolipoprotein
B, very low density apolipoprotein II (apoVLDLII), serum albumin, and transferrin at levels detectable by Northern blot analysis.
Regulation of apoVLDLII mRNA by estrogen occurred in an appropriate time-and dose-dependent manner in serum-free cultures
of the LMH cells. The expression of apoVLDLII mRNA in serum-free culture was at least 100-fold higher than that expressed
in cultures containing 10% serum. The level of estrogen receptors in LMH cells cultured with 10% serum was approximately 2000
receptors per cell, and in serum-free culture approximately 1000 receptors per cell. When these cells were transfected with
estrogen receptor DNA and cultured in serum-free medium, apoVLDLII mRNA was decreased relative to that expressed in cells
transfected with a control plasmid. These results indicate that when the LMH cells are cultured without serum, estrogen receptors
are not the limiting factor for the expression of the apoVLDLII gene. 相似文献
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Expression of endogenous and transfected apolipoprotein II and vitellogenin II genes in an estrogen responsive chicken liver cell line 总被引:4,自引:0,他引:4
R Binder C C MacDonald J B Burch C B Lazier D L Williams 《Molecular endocrinology (Baltimore, Md.)》1990,4(2):201-208
A recently described chicken liver cell line, LMH, was characterized to evaluate responsiveness to estrogen. Expression of the endogenous apolipoprotein (apo) II gene was induced by 17 beta-estradiol when LMH cells were cultured with chicken serum. The response was low and yielded apoll mRNA at only 0.3% of the level seen in estrogenized rooster liver. Higher levels of apoll mRNA were achieved when LMH cells were transiently transfected with an expression plasmid for estrogen receptor. A transfected apoll gene was strongly expressed only when cotransfected with receptor. Expression of the endogenous vitellogenin (VTG) II gene was not detected. However, when cotransfected with a receptor expression plasmid, VTG II reporter plasmids were expressed in LMH cells in response to 17 beta-estradiol. These results suggest that estrogen responsiveness of LMH cells is limited by the availability of functional receptor. Low levels of estrogen receptor mRNA were detected in LMH cells, and receptor binding sites and mRNA were greatly increased following transient transfection with a receptor expression plasmid. Using this transient transfection protocol, several VTG II reporter plasmids were compared in LMH cells and chick embryo fibroblasts. A plasmid containing VTG II estrogen response elements linked to a heterologous promoter was regulated by estrogen in both cell types. In contrast, reporter plasmids containing the VTG II promoter were regulated by estrogen in LMH cells but were not expressed at all in chick embryo fibroblasts. These results suggest that regulation of the VTG II gene involves cell type-specific elements in addition to estrogen response elements.(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
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Identification of a negative regulatory region 5' of the human apolipoprotein B promoter 总被引:5,自引:0,他引:5
B Paulweber A R Brooks B P Nagy B Levy-Wilson 《The Journal of biological chemistry》1991,266(32):21956-21961
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Structure and expression of the human apolipoprotein A-IV gene 总被引:8,自引:0,他引:8
N A Elshourbagy D W Walker Y K Paik M S Boguski M Freeman J I Gordon J M Taylor 《The Journal of biological chemistry》1987,262(17):7973-7981
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Cloning and structural characterization of an estrogen-dependent apolipoprotein gene 总被引:4,自引:0,他引:4
ApoVLDLII is a major polypeptide component of avian very low density lipoprotein. Its production in the liver of the maturing female chick is developmentally synchronized with vitellogenesis. The apoVLDLII gene is normally dormant in males but can be activated by the administration of estrogen. In these studies, we describe the isolation of three recombinant bacteriophages that contain apoVLDLII genes. The genes appear to be identical from preliminary restriction analyses, although heterogeneity in flanking sequences is evident. The structure of the gene has been characterized and its organization correlated with the structure of the apoVLDLII polypeptide. 相似文献
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Comparative analysis of sequences at the 5' end of the human and mouse apolipoprotein B genes 总被引:3,自引:0,他引:3
E H Ludwig B Levy-Wilson T Knott B D Blackhart B J McCarthy 《DNA and cell biology》1991,10(5):329-338
A comparison was made between the DNA sequences in two regions of the mouse and the human apolipoprotein B genes: the 5'-flanking sequence and the region between the first exon and the second intron. Considerable homology was observed, particularly in the immediate 5' region and in the second intron. Because promoter and enhancer elements have been previously localized to these regions in the human apolipoprotein B gene, it is proposed that regions of conserved base sequence delineate binding regions for regulatory proteins. In some cases, contiguous regions of homology are longer than expected for regions designed as recognition sites for individual nuclear proteins, and may define regions recognizable by a cluster of interacting proteins. Both the human and mouse genes contain repetitive elements and a hypervariable dinucleotide repeat. 相似文献
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Zhou L Nian M Gu J Irwin DM 《American journal of physiology. Regulatory, integrative and comparative physiology》2006,290(3):R634-R641
The mammalian proglucagon gene is expressed in pancreatic islet A-cells, intestinal L-cells, and select neurons of the brain, where posttranslational processing results in the liberation of a unique profile of peptides. Despite the importance of proglucagon-derived peptides in human biology, little is known about the regulation of the human gene, as the rat gene has been the preferred model for understanding the regulation of proglucagon gene expression. Previously, we have shown that although the immediate promoter region of the rat proglucagon gene is sufficient for expression in pancreatic islet cells, the homologous human proglucagon promoter sequences are not sufficient. We have now used a comparative genomic approach to identify noncoding sequences near the human proglucagon gene that are conserved among mammals, and thus potentially are regulatory sequences. Our alignments identified three evolutionarily conserved noncoding regions (ECR), one is the immediate promoter region (ECR1), the second is about 5 kb 5' to the mRNA start site (ECR2), and the third is near the 3' end of the first intron (ECR3). Our in vitro transient transfection assays with reporter gene constructs that include the human ECR3 support expression in rodent islet cell lines. Complementary studies with transgenic mice possessing a reporter gene regulated by a human proglucagon gene promoter-intron 1 (including ECR3) sequences express the reporter gene in the pancreas, as well as the intestine and selected neurons. These studies suggest that conserved sequences within intron 1 of the human proglucagon gene are important for expression in the pancreas. 相似文献
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