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Escherichia coli glutaminyl-tRNA synthetase. I. Isolation and DNA sequence of the glnS gene 总被引:17,自引:0,他引:17
F Yamao H Inokuchi A Cheung H Ozeki D S?ll 《The Journal of biological chemistry》1982,257(19):11639-11643
We have isolated a lambda-transducing phage carrying the gene (glnS) for Escherichia coli glutaminyl-tRNA synthetase. The location of the glnS gene within the 13.5-kilobase E. coli DNA transducing fragment was determined by genetic means. The glnS gene was recloned into plasmid pBR322 and its nucleotide sequence was established. The DNA sequence translates to a protein of 550 amino acids. 相似文献
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Escherichia coli glutaminyl-tRNA synthetase. II. Characterization of the glnS gene product 总被引:15,自引:0,他引:15
P Hoben N Royal A Cheung F Yamao K Biemann D S?ll 《The Journal of biological chemistry》1982,257(19):11644-11650
Glutaminyl-tRNA synthetase has been purified by a simple, two-column procedure from an Escherichia coli K12 strain carrying the glnS structural gene on plasmid pBR322. The primary sequence of this enzyme as derived from the DNA sequence (see accompanying paper) has been confirmed. Manual Edman degradation was used to identify the NH2-terminal sequence of the protein. Oligopeptides scattered throughout the primary sequence of glutaminyl-tRNA synthetase were sequenced by the gas chromatographic-mass spectrometric method and matched to the theoretical peptides derived from the translated DNA sequence. The expected carboxyl terminus at position 550 was verified by carboxypeptidase B digestion. The primary sequence of glutaminyl-tRNA synthetase contains no extensive sequence repeats. A search was made for sequence homologies between this enzyme and the few other aminoacyl-tRNA synthetases for which primary sequences are available. A single homologous region is shared by at least three of the synthetases examined here. 相似文献
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Regulation of gene expression in flux balance models of metabolism. 总被引:10,自引:0,他引:10
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