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1.
Gene Duplication within the Family Salmonidae: Disomic Inheritance of Two Loci Reported to Be Tetrasomic in Rainbow Trout 总被引:3,自引:1,他引:2
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We describe our studies of the genetics of allelic variation for NADP-dependent isocitrate dehydrogenase (IDH) in rainbow trout (Salmo gairdneri). Five populations of rainbow trout were studied to determine the phenotypic distribution of IDH; 453 progeny from a number of controlled matings were examined to determine the nature of inheritance of these alleles. The variation was found to be the result of four alleles producing protein subunits of differing electrophoretic mobilities. Progeny from crosses clearly demonstrated the presence of two disomic loci controlling the variation, rather than one tetrasomic locus as had been previously reported. These findings support our contention that the hypothesis of a tetraploid event in salmonid evolution should not be uncritically accepted. 相似文献
2.
Genetic Analysis of Liver Neuraminidase Isozymes in RATTUS NORVEGICUS: Independent Control of Neu-1 and Neu-2 Phenotypes
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Paul B. Samollow John L. Vandeberg Allen L. Ford Heinz W. Kunz Thomas J. Gill III 《Genetics》1986,114(1):247-258
Two recently identified isozymes of neuraminidase in rat liver were examined for transmission patterns and linkage relationships, and for variation among inbred strains. The isozymes, designated neuraminidase-1 (NEU-1) and neuraminidase-2 (NEU-2), exhibited no electrophoretic mobility variants among the 22 inbred strains examined, but did possess striking interstrain variation in activity phenotypes on electrophoretic gels. The results of a backcross analysis involving the KGH and ACP strains revealed that NEU-1 and NEU-2 phenotypes are independently controlled, each by a single autosomal locus with additively acting alleles. The two loci are unlinked to one another, but the gene controlling NEU-1 is tightly linked to RT1, the rat major histocompatibility complex. This gene is almost certainly identical to Neu-1, a gene identified previously through its effect on "total" activity levels of liver neuraminidase as determined by fluorometric assay of tissue homogenates. NEU-2 and the gene controlling its phenotype were not detected by the fluorometric technique. We designate the genes controlling the NEU-1 and NEU-2 phenotypes as Neu-1 and Neu-2, respectively. Data from this and other studies place Neu-1 between Glo-1 and dw-3. The location of Neu-2 is unknown. 相似文献
3.
Donald H. Les James A. Reinartz Elizabeth J. Esselman 《Evolution; international journal of organic evolution》1991,45(7):1641-1650
Aster furcatus is a rare, self-incompatible plant with fewer than 50 known populations throughout its range. We verified self-incompatibility in A. furcatus by conducting experimental self- and cross-pollinations and by examining seed set in a small population comprised of a single clonal genet. We examined variation at 22 electrophoretic loci in 23 populations of A. furcatus from across its range in Wisconsin, Illinois, Indiana, and Missouri. Except for two rare alleles found in single individuals in three populations, all loci but one of those examined were fixed for single alleles. The only variable locus (triosephosphate isomerase, TPI-1) tended to exhibit genotype frequencies in Hardy-Weinberg equilibrium or with a slight excess of heterozygotes. Although overall gene diversity was extremely low, TPI genotype frequencies were indicative of an outcrossing plant. We examined the subpopulation genetic structure among clonal plants within one Wisconsin population in greater detail. F statistics indicated that much of the genetic variation at the polymorphic TPI locus was due to differentiation among populations. We discuss the implications of self-incompatibility and low levels of genetic variation for the evolution and conservation of Aster furcatus and other rare plants with similar breeding systems. 相似文献
4.
Scott C. France Nicholas Tachino Thomas F. Duda Jr. Robert A. Shleser Stephen R. Palumbi 《Marine biotechnology (New York, N.Y.)》1999,1(3):261-268
Intron sequences from the elongation factor-1α (EF1α) gene from the marine shrimp Penaeus vannamei reveal extensive variation even among inbred populations of hatchery-raised shrimp. Among 44 individuals analyzed, we found
13 alleles varying by up to 7.5% sequence differences, and including several allele-diagnostic insertions and deletions. High
heterozygosity contrasts with low genetic variation at allozyme loci, but we observed up to four alleles per individual, suggesting
that we have identified two separate, polymorphic loci. We partitioned the observed alleles into two groups representing hypothetical
duplicated loci. However, the alleles are so similar to one another that a phylogenetic analysis does not cluster them into
monophyletic groupings. A possible explanation is that concerted evolution is acting to homogenize genetic variation among
these two putative loci.
Received July 17, 1998; accepted November 23, 1998. 相似文献
5.
The evolutionary relationship between gene-structure and gene-regulation variation was examined by using data on allozyme
and activity variation for a group of enzyme loci inDrosophila melanogaster. No significant correlation between the two kinds of variation was found, although the present data set is small and probably
not sensitive for tests of association. Various hypotheses of association between the two types of variation are discussed
and it is shown that any association between gene-structure and gene-regulation variation (positive or negative) would be
sensitive to distortion by repeated bottlenecks and genetic drift. Furthermore, random forces would affect gene-structure
(allozyme) variation, which is often controlled by one gene, more severely than enzyme activity variation, which is usually
controlled by many genes. Measurements of activity variation would appear to provide an evolutionarily more stable and reliable
estimate of. loci-specific ‘molecular flexibility’ than measurements of structural variation. It is suggested that locus-specific
gene regulation studies employing a set of major alleles would provide a better test of association between gene-structure
and gene-regulation variation. The relationship between gene regulation and quantitative variation is discussed and a number
of population-genetic questions related to regulatory gene variation are formulated. 相似文献
6.
Twenty enzyme loci were examined in the diploid species ofTriticum andAegilops for allelic variation by starch gel electrophoresis. SectionSitopsis, including the five species,Ae. speltoides, Ae. lingissima, Ae. sharonensis, Ae. bicornis andAe. searsii form a close subgroup withAe. speltoides slightly removed from the others.T. monococcum s. lat., was found to be closest to the species of theSitopsis group.Ae. comosa, Ae. umbellulata andAe. uniaristata form a second subgroup withAe. caudata most closely related to these species.Ae. squarrosa appears almost equally related to all of the species, showing no special affinity for any one species group. Nineteen out of twenty loci examined were polymorphic with a mean of 6.7 alleles per locus. Species could be, for most loci, characterized by the presence of predominant alleles. A conspicious genetic characteristic ofTriticum-Aegilops is the sharing of these predominant alleles between species. Within species variation is characterized by a diffuse distribution of secondary alleles. 相似文献
7.
Gary E. Hart 《Biochemical genetics》1969,3(6):617-625
By means of the zymogram technique, two types of electrophoretic patterns were found for the enzyme alcohol dehydrogenase (ADH) in Triticum dicoccum, a tetraploid species of wheat. Each strain examined showed three bands of ADH activity. The two types of patterns found differed with respect to the relative electrophoretic mobilities and staining intensities of the bands. Evidence that the variation between the patterns is controlled at a single gene locus by two codominant alleles was obtained from appropriate genetic crosses. Heterozygotes for the allelic difference have five bands of ADH activity. These probably represent six different forms of the enzyme, two of which have coincident electrophoretic mobilities. These observations support the hypothesis that ADH exists as a dimer in T. dicoccum. It is probable that the enzyme subunits are coded for by duplicated ADH gene loci, each of which was contributed to the original tetraploid wheats by one of the two diploid progenitors of that group.This paper is Technical Article No. 7983, Texas Agricultural Experiment Station. 相似文献
8.
We isolated six microsatellite markers from the partial genomic libraries of two Sorex shrews, S. unguiculatus and S. caecutiens, and examined their allelic variation. All loci showed high allelic variation ranging from 15 to 19 alleles and all but one locus conformed to Hardy–Weinberg expectations in the species where the loci were isolated. Cross-species amplifications showed that all primers derived from S. unguiculatus were useful for S. caecutiens, while among primer sets derived from S. caecutiens only one was useful for S. unguiculatus. Accordingly, at least five microsatellite markers were useful in S. caecutiens and three in S. unguiculatus. 相似文献
9.
P. Garcia Dr. M. Pérez de la Vega C. Benito 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1982,61(4):341-351
Summary Genetic analyses were conducted on peroxidase of the embryo and endosperm of seeds of one open pollinated and six inbred lines of cultivated rye (Secale cereale L.), and one line of Secale vavilovii Grossh. The analyses of the individual parts of the S. cereale seed yield a total of 14 peroxidase isozymes. Isozymes m, a, b, c, d, e, f and g (in order from faster to slower migration) were found in the embryo plus scutellum, while isozymes 1, 2, 3, 4, 5 and 6 (also from faster to slower migration) were peculiar of the endosperm. S. vavilovii has isozymes m, c1, d, e, f and g in its embryo plus scutellum, and isozyme 2 in the endosperm. Segregation data indicated that at least 13 different loci would be controlling the peroxidase of S. cereale. Isozymes a and b are controlled by alleles of the same locus, all the other loci have one active and dominant allele coding for one isozyme, and other null and recessive allele. The estimation of linkage relationships shows that five endosperm loci are linked, and tentative maps are shown. A possible dosage effect and the existence of controlling gene(s) for endosperm isozyme 4 is reported. All these data and the high frequency of null alleles found are discussed in relation to recent reports. 相似文献
10.
C.-T. Jean S.-C. Lee C.-F. Hui C.-T. Chen 《Journal of Zoological Systematics and Evolutionary Research》1995,33(2):49-53
The phylogenetic relationship of five species belonging to the subfamily Sparinae was investigated using starch-gel electrophoretic analysis of enzymes in skeletal muscle, heart, eye, liver, and sarcoplasmic protein in skeletal muscle. A total of 38 loci with 105 alleles coding for 25 enzymes and general protein were analysed and scored to calculate the proportion of polymorphic loci at the 95% level (P95), mean heterozygosity over loci (H), average number of alleles per locus (Na), and genetic distance (D). P95 values obtained ranged from 13.16 to 18.42%, H values ranged from 0.042 to 0.057, Na values ranged from 1.21 to 1.42, and an average D value of 0.305 (0.216-0.386) between congeneric species and 0.801 (0.614-0.896) between two consubfamilial genera, Acanthopagrus and Sparus, was found. A phenogram constructed on the basis of genetic data was similar to that based on morphometric characters at the genus level. However, discordance does exist among species of Acanthopagrus between the two sets of phenograms constructed. The results of this study support the niche-width-variation hypothesis. 相似文献
11.
Richard J. Hoffmann 《Biochemical genetics》1981,19(1-2):145-154
Electrophoretic surveys have demonstrated that populations of the sea anemone Metridium senile along the northeast coast of the United States are polymorphic at four enzyme loci. Phosphoglucose isomerase (PGI) has two alleles in most populations, phosphoglucomutase (PGM) has three alleles, and two leucine aminopeptidase loci have two common alleles each. Phosphoglucose isomerase displays clinal variation and an apparent association with environmental temperature. Phosphoglucomutase shows clinal variation north of Cape Cod for two of the three alleles, while the two leucine aminopeptidase loci are not clinal. All loci show a great deal of variation in populations on Cape Cod, but there is no apparent systematic pattern to this variation. Temperature may be a selective agent in the maintenance of the PGI and PGM clines, although other possibilities cannot presently be completely excluded.Supported by Grant T-4 from the Health Research and Services Foundation, NSF DEB77-14442, NIH GM25809, and NIH GM28024. 相似文献
12.
John J. Hutton 《Biochemical genetics》1971,5(4):315-331
The erythrocyte glucose 6-phosphate dehydrogenase activity characteristic of each of 16 inbred mouse strains falls into one of three distinct classes. Strains C57L/J and C57BR/cdJ represent the low activity class: strains A/J and A/HeJ represent the high activity class; other strains have intermediate activities. There is no evidence that structural variation is responsible for the variation in G6PD activity, since partially purified enzyme from each class has the same thermal stability, pH-activity profile, Michaelis constants for G6P and NADP, electrophoretic mobility, and activity using 2-deoxy d-glucose 6-phosphate as substrate. The activities of 6-phosphogluconate dehydrogenase and glucose phosphate isomerase do not differ in erythrocytes of the three G6PD activity classes. Young red cells have higher G6PD activities than old red cells and there is evidence that the intracellular stability of the enzyme is reduced in red cells of strain C57L/J. G6PD activities in kidney and skeletal and cardiac muscle from animals with low red cell G6PD are slightly lower than the activities in kidney and muscle from animals with high red cell G6PD activity. The quantitative differences in red cell G6PD activity are not regulated by X-linked genes, but by alleles at two or more autosomal loci. A simple genetic model is proposed in which alleles at two unlinked, autosomal loci, called Gdr-1 and Gdr-2 regulate G6PD activity in the mouse erythrocyte. 相似文献
13.
C. W. Deren K. H. Quesenberry 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1989,78(6):825-830
Summary Induction of flowering by photoperiod was studied in the parental, F1, F2, and reciprocal backcross generations of crosses between three photoperiod-responsive Aeschynomene americana L. lines. Generation means appeared additive. Analysis with Mather and Jinks' scaling tests showed little or no epistasis and indicated that an additive-dominance model was adequate. Partitioning components of variation revealed that nearly all variation was additive genetic with dominance and environmental variation negligible. An additive genetic model with two loci, each with two alleles and all alleles having equal net effect, was tested using Power's partitioning method. Results demonstrated that the model fit the data and that there is a major additive genetic system controlling flowering in these crosses, with minor genetic and environmental influences present. Selection for flowering at a desired day length should be feasible.Accepted by A.R. HallauerFlorida Agricultural Experiment Station, Journal Series No. 9251 相似文献
14.
Festenstein originally described the Mls locus as a single dominant autosomal gene with four alleles which mapped in the 13th linkage group of chromosome 1. We subsequently presented evidence which indicated that the mixed leukocyte reaction stimulatory products of DBA/2 and CBA/J were controlled by two independently segregating Mls loci. Recently, Mls
d of CBA/J was shown to be composed of Mls
a of AKR and Mls
c of C3H. In the present report, classic segregation data is presented which indicates that Mls
c of C3H is controlled by three independently segregating loci. As defined by stimulatory patterns of numerous cell lines, we postulate the following: either one of the loci is shared with BALB.K, CE, C58, and partially with MA/MyJ, one is shared with CBA/H and CBA/J, and one is shared with BALB.K, CBA/J, and partially with CE; or the groups of shared determinants are controlled by different alleles of unique loci (or locus). In any event, Mls
c appears to be composed of at least three independently segregating loci; the number of alleles/locus is being investigated. In addition, C3H was stimulated by BALB.K (both were recently postulated to be Mls
c ); this epitope was shared with CBA/J, CBA/H, AKR/Cum, Ma/MyJ, and C58/J. 相似文献
15.
Matthew A. Parker 《Evolution; international journal of organic evolution》1988,42(2):239-247
Polymorphism existed at 58% of the enzyme loci examined (11/19) in one population of the highly self-pollinated annual legume Amphicarpaea bracteata. Due to extreme gametic disequilibrium among loci, genetic variation in this population was structured into a small number of multilocus genotypes. Over 97% of the plants sampled could be grouped into two classes (biotypes “A” and “B”), each consisting of a few highly similar genotypes. The two classes had mutually exclusive sets of alleles at nine loci. These classes differed sharply in their disease resistance toward one isolate of the specialist fungal pathogen Synchytrium decipiens from their native habitat. All biotype A plants were strongly susceptible, and all biotype B plants were resistant. When plants of both biotypes were exposed to this pathogen in a greenhouse, the resistant biotype (B) exhibited a significantly higher growth rate. The strong association between plant disease-resistance phenotypes and allozyme variants implies that pathogen attack could be a major selective agent influencing the evolution of neutral or near-neutral alleles at enzyme loci in this plant. 相似文献
16.
E. G. Zimmerman 《Genetica》1988,76(2):153-159
Genetic variation at eight polymorphic loci was examined in a population of a subterranean rodent, the plains pocket gopher (Geomys bursarius), sampled over a 10-yr period. Two loci exhibited relatively minor changes in gene frequencies, while the remaining loci demonstrated major shifts in predominant alleles, loss of minor alleles, and addition of alleles due to migration. Significant deviations from Hardy-Weinberg expectations, concomitant to heterozygote deficiencies, were observed for several loci. Temporal heterogeneity, as measured by FST, was high and comparable to that exhibited by local populations sampled over relatively short periods of time. The high degree of temporal genetic variation is consistent with observations that fossorial rodents occur in locally isolated populations with small effective population sizes that are subject to genetic drift, bottlenecking, and inbreeding. 相似文献
17.
Homoeologous loci control the accumulation of seed glucosinolates in oilseed rape (Brassica napus). 总被引:3,自引:0,他引:3
The genetic control of seed glucosinolate content in oilseed rape was investigated using two intervarietal backcross populations. Four QTLs segregating in the population derived from a Brassica napus L. 'Victor' x Brassica napus L. 'Tapidor' cross, together accounting for 76% of the phenotypic variation, were mapped. Three of these loci also appeared to control the accumulation of seed glucosinolates in a Brassica napus L. 'Bienvenu' x 'Tapidor' cross, and accounted for 86% of the phenotypic variation. The three QTLs common to both populations mapped to homoeologous regions of the B. napus genome, suggesting that seed glucosinolate accumulation is controlled by duplicate genes. It was possible to extend the comparative analysis of QTLs controlling seed glucosinolate accumulation by aligning the published genetic maps generated by several research groups. This comparative mapping demonstrated that high-glucosinolate varieties often carry low-glucosinolate alleles at one or more of the loci controlling seed glucosinolate accumulation. 相似文献
18.
A. M. Figueiras M. T. González-Jaén C. Benito 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1987,73(5):683-689
Summary Genetic analyses were conducted on alkaline phosphatases of the endosperm of dry kernels and leaf acid phosphatases in four open pollinated and one inbred line of cultivated rye (Secale cereale L.). A total of seven alkaline phosphatase isozymes were observed occurring at variable frequencies in the different cultivars analyzed. We propose that at least five loci control the alkaline phosphatases of rye endosperm — Alph-1, Alph-2, Alph-3, Alph-4 and Alph-5 — all of which have monomeric behaviour. The leaf acid phosphatases are controlled by one locus and have a dimeric quaternary structure. All loci coding for alkaline phosphatase isozymes showed one active, dominant allele and one null, recessive allele, except for the locus Alph-3 which showed two active, dominant alleles and one null, recessive one. The linkage analyses suggest the existence of two linkage groups for alkaline phosphatases: one of them would contain Alph-2, Alph-4, Alph-5 and the locus/loci coding isozymes 6 and 7. This linkage group is located in the 7RS chromosome arm. The other group would include Alph-1 and Alph-3 loci, being located in the 1RL chromosome arm. Leaf acid phosphatases have been previously located in the 7RL chromosome arm. Our data also support an independent relationship between loci controlling the endosperm alkaline phosphatases and leaf acid phosphatases. 相似文献
19.
Two distinct population groups of the pond frog Rana lessonae were detected in peninsular Italy and Sicily by multilocus electrophoresis: one group inhabits the peninsula down to northern Calabria, the second occurs in southern Calabria and on Sicily. Fixed alternative alleles distinguish the two groups at 5 of the 25 loci examined; marked allele frequency differences were observed at two additional loci. On average, the two groups differ by a Nei's standard genetic distance of 0.4. A wide hybrid zone (about 120 km) occurs between the two groups, with high genotypic diversity and absence of pure parental genotypes in central Calabria. Patterns of allozyme variation suggest that at least two distinct contact and hybridization events occurred, one in the Catanzaro, the other in the Crati-Sibari plains, about 70 km to the north. Geological evidence indicates that these areas correspond to two main marine-flooded grabens that would have repeatedly interrupted or reduced genetic exchange during Plio-Pleistocene times. The finding of a fixed difference at the Mdhp-1 locus between Sicilian and Calabrian R. lessonae witnesses their continuing differentiation following their last separation by definitive opening of the Strait of Messina, about 50 000 years ago. The wide hybrid zone, the diversity of genotypes and the agreement with Hardy-Weinberg expectations suggest complete hybrid fertility. Different patterns of introgression were observed at the various loci. The pattern of allelic variation at loci in R. lessonae is paralleled by the pattern of variation in lessonae genomes of the sympatric hemiclonal hybrid Rana esculenta, into which lessonae genomes are introduced each generation as a result of hybridogenesis. 相似文献
20.
Identification of QTLs affecting traits of agronomic importance in a recombinant inbred population derived from a subspecific rice cross 总被引:59,自引:6,他引:53
J. Xiao J. Li L. Yuan S. D. Tanksley 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1996,92(2):230-244
To detect QTLs controlling traits of agronomic importance in rice, two elite homozygous lines 9024 and LH422, which represent the indica and japonica subspecies of rice (Oryza sativa), were crossed. Subsequently a modified single-seed-descent procedure was employed to produce 194 recombinant inbred lines (F8). The 194 lines were genotyped at 141 RFLP marker loci and evaluated in a field trial for 13 quantitative traits including grain yield. Transgressive segregants were observed for all traits examined. The number of significant QTLs (LOD 2.0) detected affecting each trait ranged from one to six. The percentage of phenotypic variance explained by each QTL ranged from 5.1% to 73.7%. For those traits for which two or more QTLs were detected, increases in the traits were conditioned by indica alleles at some QTLs Japonica alleles at others. No significant evidence was found for epistasis between markers associated with QTLs and all the other markers. Pleitropic effects of single QTLs on different traits are suggested by the observation of clustering of QTLs. No QTL for traits was found to map to the vicinity of major gene loci governing the same traits qualitatively. Evidence for putative orthologous QTLs across rice, maize, oat, and barley is discussed. 相似文献