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1.
Molasses melanoidin (MM) is a major pollutant in biomethanated distillery spent wash (BMDS) due to its recalcitrant properties. The 75% colour and 71% COD of MM (1,000?ppm) were reduced with developed bacterial consortium comprising Proteus mirabilis (IITRM5; FJ581028), Bacillus sp. (IITRM7; FJ581030), Raoultella planticola (IITRM15; GU329705) and Enterobacter sakazakii (IITRM16, FJ581031) in the ratio of 4:3:2:1 within 10?days at optimized nutrient. Bacterial consortium showed manganese peroxidase and laccase activity during MM decolourisation. The dominant growth of R. planticola and E. sakazakii was noted in consortium during MM decolourisation. The comparative GC-MS analysis of extracted compounds of control and degraded samples showed that most of the compounds present in control were completely utilized by bacterial consortium along with production of some metabolites. The developed bacterial consortium could be a tool for the decolourisation and degradation of melanoidin containing BMDS. 相似文献
2.
Alkalophilic bacterial consortium developed by continuous enrichment in the chemostat in presence of 4-chlorosalicylic acid
as sole source of carbon and energy contained six bacterial strains, Micrococcus luteus (csa101), Deinococcus radiothilus (csa102), csa103 (Burkholderia gladioli), Alloiococcus otilis (csa104), Micrococcus diversus (csa105), Micrococcus luteus (csa106), identified by the Biolog test method. The strains were tested for utilization of organic compounds in which one
of the strains (csa101) had higher potency to utilize dibenzofuran (DF) as sole carbon and energy source identified as Serratia marcescens on the basis of 16S rDNA. The degradation of DF by bacterial strain proceeded through an oxidative route as indicated by
2,2′3-trihydroxybiphenyl, 2-hydroxy-6-(2-hydroxyphenyl)-6-oxo-2,4-hexadienoic acid, salicylic acid, and catechol, which was
identified by gas chromatography–mass spectrometry. 相似文献
3.
Rainer -B. Volk 《Journal of applied phycology》2006,18(2):145-151
The isolation of norharmalane (3,4-dihydro-9H-pyrido(3,4-b)indole) from culture media of the cyanobacterium Nodularia harveyana is described. The minimum toxic quantities against selected cyanobacteria of this compound, of the two known cyanobacterial exometabolites 4,4′-dihydroxybiphenyl and norharmane (9H-pyrido(3,4-b)indole) and in addition of harmane (1-methyl-9H-pyrido(3,4-b)indole) were determined using a special TLC plate assay. The three β-carbolines harmane, norharmane and norharmalane were tested both as bases and as hydrochlorides. All four test compounds were found to be cytotoxic against the cyanobacterial test organisms in low quantities (0.5 to 18.0 μg). For the β-carbolines the following structure-response relationships were revealed: the double bond in position 3–4 and possibly the 1-methyl-group increased the cytotoxic effect of these indole alkaloids. Minimum toxic quantities detected for β-carboline bases were in general lower than those of equimolar amounts of the corresponding hydrochlorides. The possible applicability of both β-carbolines and biphenyls as agents in antifouling systems is discussed. 相似文献
4.
Metabolic pathways of quinoline, indole and their methylated analogs by Desulfobacterium indolicum (DSM 3383) 总被引:2,自引:0,他引:2
S. S. Johansen D. Licht E. Arvin H. Mosbæk A. B. Hansen 《Applied microbiology and biotechnology》1997,47(3):292-300
The transformation of quinoline, isoquinoline and 3-, 4-, 6- and 8-methylquinoline by Desulfobacterium indolicum was compared with that of the N-containing analogues indole and 1-, 2-, 3- and 7-methylindole. The metabolites were identified
using high-performance liquid chromatography with UV detection, thin-layer chromatography, combined gas chromatography/mass
spectrometry and proton NMR spectroscopy. All degraded compounds were initially hydroxylated at position 2 by D. indolicum. A new degradation product of quinoline was observed in the second transformation step, where 3,4-dihydro-2-quinolinone accumulated.
This ring-reduced compound was further transformed into unidentified products. The transformation pathway of indole was characterized
by well-known steps through oxindole, isatin, and anthranilic acid. No further transformation of the hydroxylated methyl analogues:
3- and 7-methyloxindole and 3- and 4-methyl-2-quinolinone, was observed within 162 days of incubation. These degradation products
accumulated in stoichiometric amounts, while 6- and 8-methyl-2-quinolinone were further degraded to 6- and 8-methyl-3,4-dihydro-2-quinolinone
in stoichiometric amounts. Isoquinoline, 2-methylquinoline and 1- and 2-methylindole were not degraded by D. indolicum. These observations indicate that a methyl group at or close to position 2 results in blockage of the microbial attack, and
that transformation of hydroxyquinolines methylated at the heterocyclic ring also was blocked or sterically inhibited. An
incomplete transformation of some methylated compounds was observed, e.g. for 3- and 6-methylquinoline and 3- and 7-methylindole,
with residual concentrations of 0.5–4 mg/l in relation to initial concentrations of 10–15 mg/l.
Received: 23 July 1996 / Received revision: 4 October 1996 / Accepted: 25 October 1996 相似文献
5.
Sikandar I. Mulla Robertcyril S. Hoskeri Yogesh S. Shouche Harichandra Z. Ninnekar 《Biodegradation》2011,22(1):95-102
A bacterial consortium capable of degrading nitroaromatic compounds was isolated from pesticide-contaminated soil samples
by selective enrichment on 2-nitrotoluene as a sole source of carbon and energy. The three different bacterial isolates obtained
from bacterial consortium were identified as Bacillus sp. (A and C), Bacillus flexus (B) and Micrococcus sp. (D) on the basis of their morphological and biochemical characteristics and by phylogenetic analysis based on 16S rRNA
gene sequences. The pathway for the degradation of 2-nitrotoluene by Micrococcus sp. strain SMN-1 was elucidated by the isolation and identification of metabolites, growth and enzymatic studies. The organism
degraded 2-nitrotoluene through 3-methylcatechol by a meta-cleavage pathway, with release of nitrite. 相似文献
6.
Isolation and characterization of aerobic bacteria capable of the degradation of synthetic and natural melanoidins from distillery effluent 总被引:1,自引:0,他引:1
Ram Naresh Bharagava Ram Chandra Vibhuti Rai 《World journal of microbiology & biotechnology》2009,25(5):737-744
Melanoidins, complex biopolymer of amino-carbonyl compounds are the major coloring and polluting constituents of distillery
wastewaters. In this study, three aerobic melanoidin-degrading bacteria (RNBS1, RNBS3 and RNBS4) were isolated from soil contaminated
with distillery effluent and characterized as Bacillus licheniformis (RNBS1), Bacillus sp. (RNBS3) and Alcaligenes sp. (RNBS4) by biochemical tests and 16S rRNA gene sequence analysis. The degradation of synthetic and natural melanoidins
was studied by using the axenic and mixed bacterial consortium. Results have revealed that the mixed consortium was more effective
compared to axenic culture decolorizing 73.79 and 69.83% synthetic and natural melanoidins whereas axenic cultures RNBS1,
RNBS3 and RNBS4 decolorized 65.88, 62.56 and 66.10% synthetic and 52.69, 48.92 and 59.64% natural melanoidins, respectively.
The HPLC analysis of degraded samples has shown reduction in peak areas compared to controls, suggesting that decrease in
color intensity might be largely attributed to the degradation of melanoidins by isolated bacteria. 相似文献
7.
A bacterial consortium capable of degrading chloroaromatic compounds was isolated from pulp and paper mill effluents by selective
enrichment on 4-chlorobenzoic acid as sole source of carbon and energy. The four different bacterial isolates obtained from
bacterial consortium were identified as Pseudomonas aeruginosa AY792969 (A), P.
aeruginosa PA01 NC (B), Pseudomonas sp. ZZ5 DQ113452 (C) and Pseudomonas sp. AY762360 (D) based on their morphological and biochemical characteristics and by phylogenetic analysis based on 16S rRNA
gene sequences. These bacterial isolates were found to be versatile in degrading a variety of chloroaromatic compounds including
fluoro- and iodobenzoic acids. P. aeruginosa PA01 NC utilized 4-chlorobenzoic acid at 2 g/l as growth substrate. Biodegradation studies have revealed that this organism
degraded 4-chlorobenzoic acid through 4-chlorocatechol which was further metabolized by ortho-cleavage pathway and the dechlorination occurred after the ring-cleavage. 相似文献
8.
High-molecular-weight (HMW) polycyclic aromatic hydrocarbons (PAHs) are pollutants that persist in the environment due to
their low solubility in water and their sequestration by soil and sediments. Although several PAH-degrading bacterial species
have been isolated, it is not expected that a single isolate would exhibit the ability to degrade completely all PAHs. A consortium
composed of different microorganisms can better achieve this. Two-liquid phase (TLP) culture systems have been developed to
increase the bioavailability of poorly soluble substrates for uptake and biodegradation by microorganisms. By combining a
silicone oil–water TLP system with a microbial consortium capable of degrading HMW PAHs, we previously developed a highly
efficient PAH-degrading system. In this report, we characterized the bacterial diversity of the consortium with a combination
of culture-dependent and culture-independent methods. Polymerase chain reaction (PCR) of part of the 16S ribosomal RNA gene
(rDNA) sequences combined with denaturing gradient gel electrophoresis was used to monitor the bacterial population changes
during PAH degradation of the consortium when pyrene, chrysene, and benzo[a]pyrene were provided together or separately in the TLP cultures. No substantial changes in bacterial profiles occurred during
biodegradation of pyrene and chrysene in these cultures. However, the addition of the low-molecular-weight PAHs phenanthrene
or naphthalene in the system favored one bacterial species related to Sphingobium yanoikuyae. Eleven bacterial strains were isolated from the consortium but, interestingly, only one—IAFILS9 affiliated to Novosphingobium pentaromativorans—was capable of growing on pyrene and chrysene as sole source of carbon. A 16S rDNA library was derived from the consortium
to identify noncultured bacteria. Among 86 clones screened, 20 were affiliated to different bacterial species–genera. Only
three strains were represented in the screened clones. Eighty-five percent of clones and strains were affiliated to Alphaproteobacteria
and Betaproteobacteria; among them, several were affiliated to bacterial species known for their PAH degradation activities
such as those belonging to the Sphingomonadaceae. Finally, three genes involved in the degradation of aromatic molecules were
detected in the consortium and two in IAFILS9. This study provides information on the bacterial composition of a HWM PAH-degrading
consortium and its dynamics in a TLP biosystem during PAH degradation. 相似文献
9.
Shail Singh B. B. Singh R. Chandra D. K. Patel V. Rai 《World journal of microbiology & biotechnology》2009,25(10):1821-1828
The consortium of Bacillus cereus (DQ002384), Serratia marcescens (AY927692) and Serratia marcescens (DQ002385) were used for pentachlorophenol (PCP) degradation. The consortia showed better overall removal efficiencies than
single strains by utilization of PCP as a carbon and energy source confirmed by pH dependent dye indicator bromocresol purple
(BCP) in mineral salt media (MSM). Mixed culture was found to degrade up to 93% of PCP (300 mg/l) as compared to single strains
(62.75–90.33%), at optimized conditions (30 ± 1°C, pH 7 ± 0.2, 120 rpm) at 168 h incubation. PCP degradation was also recorded
at 20°C (62.75%) and 37°C (83.33%); pH 6 (70%) and pH 9 (75.16%); 50 rpm (73.33%) and 200 rpm (91.63%). The simultaneous release
of chloride ion up to 90.8 mg/l emphasized the bacterial dechlorination in the medium. GC–MS analysis revealed the formation
of low molecular weight compound, i.e., 6-chlorohydroxyquinol, 2,3,4,6-tetrachlorophenol and tetrachlorohydroquinone, from
degraded sample as compared to control. 相似文献
10.
Ram Chandra Sangeeta Yadav Ram Naresh Bharagava 《World journal of microbiology & biotechnology》2010,26(4):685-692
This study deals with the optimization of bacterial degradation of pyridine raffinate by previously isolated two aerobic bacteria ITRCEM1 (Bacillus cereus) and ITRCEM2 (Alcaligens faecalis) with accession number DQ4335020 and DQ435021, respectively. The degradation of pyridine raffinate was studied by axenic and mixed bacterial consortium at different nutritional and environmental conditions after the removal of formaldehyde from pyridine raffinate (FPPR). Results revealed that the optimum degradation of pyridine raffinate was observed by mixed bacterial culture in presence of glucose (1% w/v) and peptone (0.2% w/v) at 20% FPPR, pH 7.0, temperature 30°C and 120 rpm at 168 h incubation period . The HPLC analysis of degraded pyridine raffinate samples has indicated the complete removal of α, β and γ picoline. Further, the GC–MS analysis of FPPR pyridine raffinate has shown the presence of pyrazine acetonitrile (6.74), 1,3-dioxepin (8.68), 2-pyridine carboxaldehyde (11.26), propiolactone (12.06), 2-butanol (13.10), benzenesulfonic acid (16.22) and 1,4-dimethyl pyperadine while phenol (17.64) and 3,4-dimethyl benzaldehyde as metabolic products of FPPR. 相似文献
11.
Application of Biofilm-Forming Bacteria on the Enhancement of Organophosphorus Fungicide Degradation
This study aimed to develop technology enhancing the biodegradation efficacy against organophosphorus fungicide with biofilm-forming bacteria in situ. Using the crystal violet staining method, two bacterial strains having biofilm formation capability were isolated and identified as Pseudomonas sp. C7 and Bacillus sp. E5. Compared with the culture of tolclofos-methyl degrader Sphingomonas sp. 224, biofilm formation was improved by co-inoculation with biofilm-forming bacterium Bacillus sp. E5. Evaluated in liquid culture conditions, this two-species mixed consortium was observed to degrade tolclofos-methyl more effectively than Sphingomonas sp. 224 alone, with an approximately 90% degradation efficiency within 48 h of dosing. The improved effectiveness of the consortium biofilm was reflected using soil in situ with an approximately 7% increased degradation ratio over Sphingomonas sp. 224 alone. This is the first report demonstrating improved bioremediation degradation efficacy against tolclofos-methyl exhibited by a consortium biofilm. This work presents a possible effective bioremediation strategy using a specific biofilm composition against pollutants containing organophosphorus compounds in situ. 相似文献
12.
Five tempe-derived bacterial strains identified as Micrococcus or Arthrobacter species were shown to transform the soybean isoflavones daidzein and glycitein to polyhydroxylated isoflavones by different
hydroxylation reactions. All strains converted glycitein and daidzein to 6,7,4′-trihydroxyisoflavone (factor 2) and the latter
substrate also to 7,8,4′-trihydroxyisoflavone. Three strains transformed daidzein to 7,8,3′,4′-tetrahydroxyisoflavone and
6,7,3′,4′-tetrahydroxyisoflavone. In addition, two strains formed 6,7,8,4′-tetrahydroxyisoflavone from daidzein. Conversion
of glycitein by these two strains led to the formation of factor 2 and 6,7,3′,4′-tetrahydroxyisoflavone. The structures of
these transformation products were elucidated by spectroscopic techniques and chemical degradation.
Revision received: 9 September 1995 / Accepted: 21 September 1995 相似文献
13.
We compared the metabolism of eight di- and trichlorobiphenyls by eight bacterial strains chosen to represent a broad range
of degradative activity against polychlorinated biphenyls (PCBs). The PCB congeners used were 2,3-, 2,3′-, 2,4′-, 3,3′-, 2,3,3′-,
2,4,4′-, 2,5,3′-, and 3,4,2′-chlorobiphenyl. The bacterial strains used wereCorynebacterium sp. MB1,Alcaligenes strainsA. eutrophus H850 andA. faecalis Pi434, andPseudomonas strains LB400 and H1130,P. testosteroni H430 and H336, andP. cepacia H201. The results indicated that both the relative rates of primary degradation of PCBs and the choice of the ring attacked
were dependent on the bacterial strain used. The bacterial strains exhibited considerable differences in their relative reactivity
preferences for attack on mono- and dichlorophenyl groups and in the degree to which the attack was affected by the chlorine
substitution pattern on the nonreacting ring. For MB1 the reactivity pattern was 3-≥4-≫2-chlorophenyl with no attack on 2,4-
or 2,5-chlorophenyl groups. This strain was relatively insensitive to the chlorine substitution pattern on the nonreacting
ring. Strains H1130, H430, H201, and Pi434 exhibited the same reactivity preferences as MB1, but for these strains (and for
all others tested) the chlorination pattern on the nonreacting ring had a strong effect. For strain H336 the reactivity preference
was 4-≥2->2,4-≥3-chlorophenyl, with no evidence of attack on 2,5-chlorophenyl rings. For strains H850 and LB400 the relative
reactivity was 2->2,5->3-≫2,4->4-chlorophenyl. On this basis we propose that the eight bacterial strains represent four distinct
classes of biphenyl/PCB-dioxygenase activity.
The types of products formed were largely strain-independent and were determined primarily by the chlorine substitution pattern
on the reacting ring. When the reacting ring was an unsubstituted phenyl or a 2-chlorophenyl group, the products were chlorobenzoic
acids in high yields; for a 3-chlorophenyl ring, both chlorobenzoic acids and chloroacetophenones in moderate yields; and
for a 4- or 2,4-chlorophenyl group, chlorobenzoic acids in low yields with an apparent accumulation ofmeta ring-fission product. Strains H850 and LB400 were able to degrade the 3-chlorobenzoic acid that they produced from the degradation
of 2,3′-chlorobiphenyl. We conclude that despite differences among strains in the specificity of the initial dioxygenase,
the specificities of the enzymes responsible for the subsequent degradation to chlorobenzoic acid and/or chloroacetophenone
are quite similar for all strains. 相似文献
14.
IS elements were identified in the genomes of five Acidithiobacillus ferrooxidans strains isolated from various media. IST2 elements were revealed in all the strains grown in a medium with ferrous iron,
ISAfe1 elements were detected in four strains (TFBk, TFL-2, TFV-1 and TFO). Three strains (TFV-1, TFN-d and TFO) were found
to contain IS elements, ~600 bp long. These were named preliminary as ISAfe600. Partial sequencing of the 5′- and 3′-terminal
nucleotide stretches of an ISAfe1 element in TFBk and TFL-2 strains and complete sequencing of the ISAfe1 element in the TFBk
strain has revealed nucleotide substitutions as compared to the prototype, i.e., the ISAfe1 element of an ATCC 19859 strain.
Partial sequencing of the 5′- and 3′-terminal nucleotide stretches of the IST2 elements in TFO, TFBk and TFL-2 strains has
shown numerous nucleotide substitutions when compared to the IST2 element of an ATCC 19859 strain. Complete sequencing of
the IST2 element in the TFBk strain has revealed: the divergence between the IST2 elements in the TFBk strain and the prototype
was 21.2%. Southern hybridization of EcoRI fragments of the chromosomal DNA from five A. ferrooxidans strains grown in a medium with ferrous iron using an internal region of ISAfe1, a full-length ISAfe1 or a full-length IST2
as probes has shown them to differ in the number of copies of IS elements and their localization on the chromosomes. Adaptation
to elemental sulfur in A. ferrooxidans strains caused changes in the number, intensity and localization of hybridization bands. The authors discuss the role of
IS elements in the adaptation of A. ferrooxidans to the new energy substrate.
The nucleotide sequence data reported in this paper were deposited in GenBank under accession numbers: AY823401, the ISAfe1
from A. ferrooxidans TFBk; AY825254, the IST2 from TFBk; DQ002894, the 5′-terminal nucleotide sequence of ISAfe1 from TFL-2; DQ002895, the 3′-terminal
nucleotide sequence of ISAfe1 from TFL-2; DQ005952, the 5′-terminal nucleotide sequence of IST2 from TFV-1; DQ005953, the
3′-terminal nucleotide sequence of IST2 from TFV-1. 相似文献
15.
Ram Chandra Ram Naresh Bharagava Atya Kapley Hemant J. Purohit 《World journal of microbiology & biotechnology》2009,25(12):2113-2119
Pyridine, heterocyclic aromatic compound is known to be toxic, carcinogenic and teratogenic to several living organisms. In
this study, two aerobic bacteria ITRCEM1 and ITRCEM2 capable for pyridine degradation were isolated and characterized as Bacillus cereus (DQ435020) and Alcaligenes faecalis (DQ435021), respectively. For pyridine degradation, mixed bacterial culture was found more effective compared to axenic culture
ITRCEM1 and ITRCEM2 degrading 94.23, 67.84, and 83.35% pyridine, respectively, at 144 h incubation period at pH 7.0 ± 0.1,
temp 37 ± 2°C and shaking rate 125 rpm in MSM containing 1% glucose and 0.2% peptone as carbon and nitrogen source, respectively.
The presence of phenol and formaldehyde in MSM has shown inhibitory effect on pyridine degradation whereas picoline has favored
the bacterial growths and pyridine degradation. Further, the HPLC analysis has shown the reduction in peaks compared to controls,
indicating that reduction in peak area might be largely attributed to the bacterial degradation of pyridine by bacterial catabolic
enzymes. 相似文献
16.
Hernando Pactao Bacosa Koichi Suto Chihiro Inoue 《World journal of microbiology & biotechnology》2011,27(5):1109-1117
In this study, the abilities of two microbial consortia (Y and F) to degrade aliphatic–aromatic hydrocarbon mixtures were
investigated. Y consortium preferentially degraded the aromatic hydrocarbon fractions in kerosene, while F consortium preferentially
degraded the aliphatic hydrocarbon fractions. Degradation experiments were performed under aerobic conditions in sealed bottles
containing liquid medium and n-octane or n-decane as representative aliphatic hydrocarbons or toluene, ethylbenzene or p-xylene as representative aromatic hydrocarbons (all at 100 mg/l). Results demonstrated that the Y consortium degraded p-xylene more rapidly than n-octane. It degraded toluene, ethylbenzene and p-xylene more rapidly than decane. In comparison, the F consortium degraded n-octane more rapidly than toluene, ethylbenzene or p-xylene, and n-decane more rapidly than toluene, ethylbenzene or p-xylene. 16S rRNA gene sequencing revealed that the Y consortium was dominated by Betaproteobacteria and the F consortium by Gammaproteobacteria, and in particular Pseudomonas. This could account for their metabolic differences. The substrate preferences of the two consortia showed that the aliphatic–aromatic
hydrocarbon binary mixtures, especially the n-decane–toluene/ethylbenzene/p-xylene pairs, reflected their degradation ability of complex hydrocarbon compounds such as kerosene. This suggests that aliphatic–aromatic
binary systems could be used as a tool to rapidly determine the degradation preferences of a microbial consortium. 相似文献
17.
Bacterial decolorization of black liquor in axenic and mixed condition and characterization of metabolites 总被引:1,自引:0,他引:1
The pulping byproducts (black liquor) cause serious environmental problem due to its high pollution load. In order to search
the degradability of black liquor, the potential bacterial strains Citrobacter freundii (FJ581026) and Citrobacter sp. (FJ581023) were applied in axenic and mixed condition. Results revealed that the mixed bacterial culture are more effective
than axenic condition and can reduce 82% COD, 79% AOX, 79% color and 60% lignin after 144 h of incubation period. Additionally,
the optimum activity of lignin degrading enzyme was noted at 96 h and characterized as manganese peroxidase (MnP) by SDS–PAGE
analysis. Further, the HPLC analysis of control and bacterial degraded sample has shown the reduction as well as shifting
of peaks compared to control indicating the degradation as well as transformation of compounds of black liquor. The comparative
GC–MS analysis of control and degraded black liquor revealed that along with lignin fragment some chlorophenolic compounds
2,4,6-trichlorophenol, 2,3,4,5-tetrachlorophenol and pentachlorophenol were detected in black liquor degraded by axenic culture
whereas these chlorophenolic compounds were completely absent in black liquor degraded by mixed bacterial culture. These chlorophenol
inhibit the oxidative degradation which seems a major reason behind the low degradability of axenic degradation compared to
mixed culture. The innovation of this aerobic treatment of alkaline black liquor opens additional possibilities for the better
treatment of black liquor along with its metabolic product. 相似文献
18.
Jiranuntipon S Chareonpornwattana S Damronglerd S Albasi C Delia ML 《Journal of industrial microbiology & biotechnology》2008,35(11):1313-1321
The presence of melanoidins in molasses wastewater leads to water pollution both due to its dark brown color and its COD contents.
In this study, a bacterial consortium isolated from waterfall sediment was tested for its decolorization. The identification
of culturable bacteria by 16S rDNA based approach showed that the consortium composed of Klebsiella oxytoca, Serratia mercescens, Citrobacter sp. and unknown bacterium. In the context of academic study, prevention on the difficulties of providing effluent as well
as its variations in compositions, several synthetic media prepared with respect to color and COD contents based on analysis
of molasses wastewater, i.e., Viandox sauce (13.5% v/v), caramel (30% w/v), beet molasses wastewater (41.5% v/v) and sugarcane
molasses wastewater (20% v/v) were used for decolorization using consortium with color removal 9.5, 1.13, 8.02 and 17.5%,
respectively, within 2 days. However, Viandox sauce was retained for further study. The effect of initial pH and Viandox concentration
on decolorization and growth of bacterial consortium were further determined. The highest decolorization of 18.3% was achieved
at pH 4 after 2 day of incubation. Experiments on fresh or used medium and used or fresh bacterial cells, led to conclusion
that the limitation of decolorization was due to nutritional deficiency. The effect of aeration on decolorization was also
carried out in 2 L laboratory-scale suspended cell bioreactor. The maximum decolorization was 19.3% with aeration at KLa = 2.5836 h−1 (0.1 vvm). 相似文献
19.
Surjeet Singh Poonam Singh S. K. Awasthi Anjali Pandey Leela Iyengar 《World journal of microbiology & biotechnology》2008,24(6):841-847
A variety of environmental inocula were tested for the development of 2-aminobenzenesulfonate (2-ABS, Orthanilic acid) degrading
bacterial enrichment. A bacterial consortium (BC), which could utilize 2-ABS as the sole carbon and energy source, could only
be developed from the sludge derived from a wastewater treatment unit of a large chemical industry manufacturing nitro and
aminoaromatics. BC consisted of two bacterial strains. Based on 16S rDNA sequence analysis, these strains were identified
to be belonging to the genus, Acinetobacter and Flavobacterium. The consortium could degrade 1,000 mg l−1 2-ABS within 40 h. Evidence for the extensive mineralization of 2-ABS, during the growth of BC, was derived from U.V-spectral
and total organic carbon analysis. BC was highly specific for 2-ABS, as other benzene sulfonates tested in this study, including
other ABS isomers, were not utilized as growth substrates. 2-ABS removal pattern in the presence of glucose was significantly
influenced by acclimation characteristics of the culture. Consortium adapted to 2-ABS/glucose demonstrated the concomitant
removal of both substrates, whereas glucose exerted catabolic repression on 2-ABS removal with glucose adapted culture. Presence
of chloramphenicol inhibited 2-ABS degradation by cells, pregrown on succinate, indicating that the 2-ABS degrading enzymes
are inducible in nature. Thus the presence of 2-ABS is essential for maintaining the high degradation potential. This enrichment
culture can find an application in the treatment of 2-ABS containing wastewaters. 相似文献
20.
Liz Juan Antonio Zermeño-Eguia Jan-Roblero Janet de la Serna Javier Zavala-Díaz de León Arturo Vera-Ponce Hernández-Rodríguez César 《World journal of microbiology & biotechnology》2009,25(1):165-170
An indigenous polychlorinated biphenyl (PCB)-degrading bacterial consortium was obtained from soils contaminated by transformer
oil with a high content of PCBs. The PCB degrader strains were isolated and identified as Brevibacterium antarcticum, Pandoraea pnomenusa, and Ochrobactrum intermedium by 16S rRNA gene sequence phylogenetic analysis. The PCB-degrading ability of the consortium and of individual strains was
determined by using GC/MS. The PCB-degrading capacities of the consortium were evaluated for three concentrations of transfomer
oil ranging from 55 to 152 μM supplemented with 0.001% biphenyl and 0.1% of Tween 80 surfactant. PCB biodegradation by the
consortium was favored in the presence of both additives and the greatest extent of biodegradation (67.5%) was obtained at
a PCB concentration of 55 μM. Each bacterial species exhibited a particular pattern of degradation relating to specific PCB
congeners. Isolated strains showed a moderate degradation capability towards tetra-, hepta-, and octa-chlorobiphenyls; although
no effect on penta-, hexa-, and nona-chlorobiphenyls was observed. Recently, PCB degradation capacity was recognized in a
Pandorea member; however, this is the first study that describes the ability of Brevibacterium and Ochrobactrum species to degrade PCBs. 相似文献