中国苦苣苔科植物的多样性与地理分布（附表）

物种多样性编目是开展生物多样性保护的重要基础,该研究结合最新分子系统学研究成果以及近年来发表的新资料,对中国苦苣苔科植物多样性和地理分布数据进行了统计和分析.结果表明:中国苦苣苔科植物共有44属671种(含种下单位,下同),其中特有属11个;特有种573种,占总种数的85.39%.种数最多的10个属依次为广义报春苣苔属(180种)、广义马铃苣苔属(122种)、石蝴蝶属(39种)、半蒴苣苔属(39种)、芒毛苣苔属(38种)、长蒴苣苔属(35种)、石山苣苔属(31种)、吊石苣苔属(31种)、蛛毛苣苔属(28种)、汉克苣苔属(22种).在地理分布上,种数排名前10的省份(区)有广西(260种,33属)、云南(236种,30属)、贵州(96种,28属)、广东(93种,17属)、四川(85种,21属)、湖南(58种,13属)、西藏(39种,9属)、湖北(29种,15属)、福建(26种,13属)、江西(25种,9属).含中国特有苦苣苔科植物的属中排前10位的分别为广义报春苣苔属(178种)、广义马铃苣苔属(119种)、石蝴蝶属(37种)、半蒴苣苔属(35种)、石山苣苔属(30种)、长蒴苣苔属(29种)、吊石苣苔属(23种)、蛛毛苣苔属(19种)、芒毛苣苔属(19种)、汉克苣苔属(11种).这表明中国南部和西南部是苦苣苔科植物的一个分布中心,特别是石灰岩地区有着高度的物种多样性和特有性,广义报春苣苔属、广义马铃苣苔属、石蝴蝶属、半蒴苣苔属、石山苣苔属、吊石苣苔属等为我国典型的优势属.此外,根据目前的研究现状,还对我国苦苣苔科植物资源的调查、分类学和系统发育研究、保护和可持续利用等进行了讨论.     

New species of alderfly genus Sialis (Megaloptera: Sialidae) from China and Vietnam,with a key to species of Sialis from Asia

The alderfly genus Sialis is the most species‐rich group of the family Sialidae. However, discovery of new Sialis species is becoming difficult because of previous good faunal exploration. In this paper, we describe two new Sialis species from the southern part of East Asia, S. australis and S. jiyuni, based on materials from historical and recent collections. These two species are closely related to the insular species S. kumejimae and are among the early diverged species within Sialis. Presently, 32 species of Sialis are recorded from Asia. A key to all the Sialis species from Asia is provided.     

基于可培养方法分析云南腾冲小空山火山谷芽胞杆菌分布特征

【目的】为了解云南腾冲小空山火山谷土壤中可培养芽胞杆菌种类分布特征。【方法】采用可培养手段对小空山火山谷阳坡、谷底和阴坡土壤中的芽胞杆菌进行分离培养,根据16S rRNA基因序列同源性对分离菌株进行鉴定,并分析系统发育地位。利用Canoco5软件分析采样点芽胞杆菌种类分布特征与土壤样品理化性质的相关性。【结果】从火山谷土壤样品中共分离获得180株芽胞杆菌,16S rRNA基因测序鉴定结果表明分离菌株隶属于芽胞杆菌纲2个科(芽胞杆菌科和类芽胞杆菌科)、6个属、34个种,其中芽胞杆菌属(Bacillus) 11个种,类芽胞杆菌属(Paenibacillus) 14个种,短芽胞杆菌属(Brevibacillus)3个种,赖氨酸芽胞杆菌属(Lysinibacillus)4个种,嗜冷芽胞杆菌属(Psychrobacillus)1个种和绿芽胞杆菌属(Viridibacillus)2个种,其中7个菌株与其最近模式菌株16SrRNA相似性低于种的界定阈值(98.65%),为芽胞杆菌潜在新物种。优势属为芽胞杆菌属和类芽胞杆菌属,优势种为蕈状芽胞杆菌(Bacillusmycoides),图瓦永芽胞杆菌(Bacillustoyonensis),蜡状芽胞杆菌(Bacilluscereus),解木糖赖氨酸芽胞杆菌(Lysinibacillusxylanilyticus),蜂房类芽胞杆菌(Paenibacillusalvei)和沙地绿芽胞杆菌(Viridibacillus arenosi)。其中16个种分离自阳坡,29个种分离自阴坡,9个种分离自谷底,三者共同种类为6种。阳坡、谷底和阴坡的芽胞杆菌种群分布Bray-Curtis相似性为62.4%,多样性分析结果表明,Shannon指数(H′)大小次序为阴坡阳坡谷底。环境因子分析发现,芽胞杆菌种群分布多样性特征与其土壤的海拔高度、碳氮比和硫含量呈负相关,而和碳源和氮源含量呈正相关。【结论】从以上结果得出,云南腾冲火山谷有着较为丰富的芽胞杆菌资源,且还存在可分离培养的芽胞杆菌的潜在新物种,为利用火山微生物资源提供了保障。     

Cellulose-decomposing fungi of desert soils in Saudi Arabia

Seventy-five species in addition to 5 varieties of cellulose-decomposing which belong to 27 genera were collected during this investigation. Aspergillus (12 species + 4 varieties), Alternaria (4 species), Stachybotrys (3 species + 1 variety), and Penicillium (12 species) were of high frequency of occurrence, of which A. fumigatus, A. niger, A. terreus, A. alternata, A. tenuissima, S. atra, P. citrinum and P. corylophilum were the most common. Six genera were of moderate frequency and these were Botryotrichum (2 species), Macrophomina (1 species), Drechslera (5 species), Ulocladium (4 species), Chaetomium (7 species) and Curvularia (4 species) of which B. atrogriseum, D. spicifera, D. sativus, Ubotrytis, C. spirale and C. lunata were the most prevalent. Five and twelve genera were recovered on cellulose-Czapek's agar plates in low and rare frequency of occurrence, respectively.     

Ticks (Acari: Ixodoidea: Argasidae,Ixodidae) of China

This paper presents results of an investigation and listing of tick species found in China during a survey in all 28 provinces. This will be a step towards a definitive list of tick species and their distribution. To date, the tick fauna of this area consists of 117 species in the following families: Argasidae-Argas (7 species), Carios (4 species) and Ornithodoros (2 species); Ixodidae-Amblyomma (8 species), Anomalohimalaya (2 species), Dermacentor (12 species), Haemaphysalis (44 species), Hyalomma (6 species), Ixodes (24 species) and Rhipicephalus (8 species). Some well known ticks carrying and transmitting many infectious agents to man and domestic animals are also found in China. These include Ixodes persulcatus, Haemaphysalis longicornis, Rhipicephalus sanguineus, R. (Boophilus) microplus and Hyalomma asiaticum. It is worth mentioning that Ixodes rangtangensis Teng and Haemaphysalis xinjiangensis Teng should be relegated to a synonym of I. moschiferi and Hae. danieli, respectively. The distribution of ticks over the provinces of China is also discussed. The information on ticks in some areas such as Henan is not exhaustive.     

Updated checklist of the mosquitoes (Diptera: Culicidae) of Croatia

Improvement of morphological and molecular identification methods allows the detection of new species of mosquitoes. The mosquito fauna of Croatia currently includes 52 species, belonging to eight genera, including Anopheles (12 species), Aedes (24 species), Coquillettidia (one species), Culex (seven species), Culiseta (six species), Orthopodomyia (one species), and Uranotaenia (one species). This is an updated checklist, which includes five new species found in Croatian mosquito fauna. Two of these are invasive mosquito species, Aedes albopictus (Skuse, 1895) and Aedes japonicus (Theobald 1901), which are spreading across Europe and Croatia. The other three species, Culex laticinctus (Edwards 1913), Culex torrentium (Martini 1925), and Anopheles daciae (Linton, Nicolescu & Harbach 2004) are autochthonous species which haven't been recorded so far. Since there are several more invasive species spreading across Europe, we assume that this is not the final list.     

Systematic revision of the yucca moths in the Tegeticula yuccasella complex (Lepidoptera: Prodoxidae) north of Mexico

The yucca moths (Tegeticula and Parategeticula) are of great importance in studies of coevolution because of their obligate mutualism with their yucca hosts. Historically, three species of Tegeticula have been recognized. One of them, T. yuccasella, has been regarded as the pollinator of all but two yucca species, but morphological, molecular and biological data show that this is a large complex of monophagous and oligophagous species that differ greatly in their biology. It also includes derived ‘cheater’ species that do not pollinate their hosts and oviposit into fruits rather than flowers. Here the yuccasella complex north of Mexico is revised. The nominotypic pollinator species yuccasella is redescribed, and ten new pollinator species described: altiplanella, baccatella, carnerosanella, cassandra, elatella, maderae, mojavella, rostratella, superficiella and treculeanella. Two non-pollinating cheater species are recognized. One such species originally misidentified as a Prodoxus species, then synonymised with yuccasella, is re-erected as the non-pollinating intermedia. In addition, the new non-pollinator species corruptrix is described.     

Leaf wax <Emphasis Type="Italic">n</Emphasis>-alkane chemotaxonomy of bamboo from a tropical rain forest in Southwest China

The leaf waxes of 23 woody bamboo species of three subgenera, Dendrocalamus, Bambusa and Dendrocalamopsis, from the Xishuangbanna tropical rain forest in Southwest China were analyzed by gas chromatography and coupled gas chromatography–mass spectrometry. The waxes of the Dendrocalamus species are dominated by C₂₇ and C₂₉ n-alkanes and their average chain length (ACL) has an average of 28.3. In marked contrast to the Dendrocalamus species, the wax composition of the Bambusa species is characterized by a broad distribution of major n-alkanes from C₂₇ to C₃₅, greater ACL values (>29) and an enhanced relative abundance (>30%) of n-alkanes with a carbon number greater than 30. Unlike the Dendrocalamus species and the Bambusa species, the Dendrocalamopsis species do not have a distinct n-alkane distribution; in some species the n-alkane distribution is comparable to that in the Bambusa species and in others to that in the Dendrocalamus species. The lipid data suggest that it might be reasonable to classify the controversial Dendrocalamopsis group as an independent genus separate from the Bambusa genus. On the basis of their smaller diversity of the dominant n-alkanes and their lower ACL values, the Dendrocalamus species might be more evolutionarily advanced than the Bambusa species, with the Dendrocalamopsis species being at an intermediate stage. The evolution and classification of the woody bamboos inferred from leaf wax n-alkanes are consistent with morphological investigations reported previously.     

Annual survey of planktonic dinoflagellates and related cysts in the Gulf of Trieste (Northern Adriatic Sea)

Abstract

A survey of planktonic dinoflagellates and related cysts was carried out in the Gulf of Trieste throughout one year from April 1992 to March 1993. 113 taxa were recovered by the analysis of phytoplankton net samples. The most represented genera were Protoperidinium (34 species), Ceratium (24 species), Dinophysis (15 species), Gonyaulax (11 species) and Prorocentrum (8 species). A particular attention was given to potentially toxic species belonging to the genera Dinophysis, Alexandrium and Prorocentrum. The highest number of species (67 species) was recorded in July, and the lowest one (18 species) in February.

33 cyst morphotypes were recorded by the analysis of sediment samples. The most represented genera were Protoperidinium (8 morphotypes), Scrippsiella (3 morphotypes), Gonyaulax (3 morphotypes) and Alexandrium (2 morphotypes); the cysts most frequently found were those of Conyaulax polyedra and Alexandrium pseudogonyaulax.     

RELATIONSHIP BETWEEN PRESENCE OF A MOTHER CELL WALL AND SPECIATION IN THE UNICELLULAR MICROALGA NANNOCHLORIS (CHLOROPHYTA)1

The cell division mechanisms of seven strains from six species of Nannochloris Naumann were analyzed and compared with those of three species of Chlorella Beijerinck and Trebouxia erici Ahmadjian using differential interference microscopy and fluorescence microscopy. Nannochloris bacillaris Naumann divides by binary fission and N. coccoides Naumann divides by budding. Distinct triangular spaces or mother cell walls were found in the dividing autosporangia of the other five strains from four species of Nannochloris, three species of Chlorella, and T. erici. In an attempt to infer an evolutionary relationship between nonautosporic and autosporic species of Nannochloris, we constructed a phylogenetic tree of the actin genes using seven strains from six species of Nannochloris, three species of Chlorella, and T. erici. Nannochloris species were polyphyletic in the Trebouxiophyceae group. Two nonautosporic species of N. bacillaris and N. coccoides were monophyletic and positioned distally. Moreover, to determine their phylogenetic position within the Trebouxiophyceae, we constructed phylogenetic tree of 18S rRNA genes adding other species of Trebouxiophyceae. Nannochloris species were polyphyletic in the Trebouxiophyceae and appeared in two different lineages, a Chlorella–Nannochloris group and a Trebouxia–Choricystis group. The nonautosporic species, N. bacillaris and N. coccoides, and three autosporic species of Nannochloris belonged to the Chlorella–Nannochloris group. Nannochloris bacillaris and N. coccoides were also monophyletic and positioned distally in the phylogenetic tree of 18S rRNA genes. These results suggest that autosporulation is the ancestral mode of cell division in Nannochloris and that nonautosporulative mechanisms, such as binary fission and budding, evolved secondarily.     

祁连山东段高寒植被群落特征及其与地形气候因子关系研究

地形气候因子与物种分布的关系影响着高寒植被群落的演替,同时对山地水源涵养林功能和结构的维持具有重要的意义。以祁连山东段水源涵养林为研究对象,基于野外植物群落物种组成及地形气候因子调查数据,运用数量分类与排序等方法,探究了高寒植物群落特征及其与地形气候因子的关系。结果表明:65个调查样方中出现181个植物种,隶属40科,124属。科的物种组成及占总物种数比例分别为菊科30个种,占16.57%;蔷薇科17个,占9.44%;禾本科13个种,占7.22%;豆科11个种,占6.11%。毛茛科10个种,占5.56%。单属种82个,占总属数的66.13%。群落层片由乔木层、灌木层和草本层组成,乔木8种,灌木25种,草本148种。乔木层优势种有青海云杉、祁连圆柏、红桦。灌木层优势种有金露梅、山生柳、匙叶小檗、高山绣线菊。草本层优势种有甘肃薹草、珠芽蓼、早熟禾、唐松草、甘青蒿。TWINSPAN将高寒植被群落划分为7个群丛类型:群丛I红桦-红花蔷薇-甘肃薹草B.albosinensis-Rosa moyesii-C.kansuensis,群丛II青海云杉-匙叶小檗-甘肃薹草P.crassifolia-B....     

Leaf anatomy and light acclimation in woody seedlings after gap formation in a cool-temperate deciduous forest

The photosynthetic light acclimation of fully expanded leaves of tree seedlings in response to gap formation was studied with respect to anatomical and photosynthetic characteristics in a natural cool-temperate deciduous forest. Eight woody species of different functional groups were used; two species each from mid-successional canopy species (Kalopanax pictus and Magnolia obovata), from late-successional canopy species (Quercus crispula and Acer mono), from sub-canopy species (Acer japonicum and Fraxinus lanuginosa) and from vine species (Schizophragma hydrangeoides and Hydrangea petiolaris). The light-saturated rate of photosynthesis (P _max) increased significantly after gap formation in six species other than vine species. Shade leaves of K. pictus, M. obovata and Q. crispula had vacant spaces along cell walls in mesophyll cells, where chloroplasts were absent. The vacant space was filled after the gap formation by increased chloroplast volume, which in turn increased P _max. In two Acer species, an increase in the area of mesophyll cells facing the intercellular space enabled the leaves to increase P _max after maturation. The two vine species did not significantly change their anatomical traits. Although the response and the mechanism of acclimation to light improvement varied from species to species, the increase in the area of chloroplast surface facing the intercellular space per unit leaf area accounted for most of the increase in P _max, demonstrating the importance of leaf anatomy in increasing P _max.     

Phylogenetic relationships between Leymus (Poaceae,Triticeae) and related diploid Triticeae species based on isozyme and genome-specific random amplified polymorphic DNA (RAPD) markers

Abstract

To investigate the phylogenetic relationships between Leymus and related diploid species of the Triticeae tribe, the esterase isozyme (EST), superoxide dismutase (SOD) isozymes, and genome-specific random amplified polymorphic DNA (RAPD) markers were used to analyze for 14 Leymus species, together with two Psathyrostachys species (Ns), three Pseudoroegneria species (St), two Hordeum species (H), Lophopyrum elongatum (E^e), Australopyrum retrofractum (W), and Agropyron cristatum (P). The data were used to construct dendrograms by means of UPGMA in the NTSYS-pc computer program. The results suggested that (1) isozyme analysis can be used in the systematic studies of these perennial Triticeae; (2) there is a close relationship between Leymus, Psathyrostachys juncea, three Pseudoroegneria species, and Lophopyrum elongatum; (3) the Ns genome-specific RAPD marker was present in all 14 polyploid species of Leymus, while the E^e and P genome-specific RAPD markers were absent in 14 polyploid species of Leymus; the St, W and H genome-specific RAPD markers were present in some species of Leymus; (4) Leymus species have multiple origins, and different Leymus species derived their genomes from different donors.     

Independent origins of tetraploid cryptic species in the fern <Emphasis Type="Italic">Ceratopteris thalictroides</Emphasis>

Ceratopteris thalictroides (L.) Brongn is a tetraploid fern species that contains at least three cryptic species, the south, the north and the third type. In this study we combined data from both chloroplast DNA (cpDNA) and nuclear DNA sequences of three diploid species and three cryptic species of C. thalictroides to unravel the origin of the cryptic species, particularly of the reticulate relationships among the diploid and tetraploid taxa in the genus Ceratopteris. Of the three diploid species examined, C. cornuta had cpDNA identical to that of the tetraploid third type plants, and this diploid species is a possible maternal ancestor of the tetraploid third type. Analysis of the homologue of the Arabidopsis thaliana LEAFY gene (CLFY1) identified ten alleles in the genus Ceratopteris, with six alleles found in C. thalictroides. The unrooted tree of the CLFY1 gene revealed four clusters. Each cryptic species showed fixed heterozygosity at the CLFY1 locus and had two alleles from different clusters of the CLFY1 tree. Consideration of the cpDNA sequences, CLFY1 genotypes of the cryptic species and CLFY1 gene tree in concert suggested that the cryptic species of C. thalictroides had originated through independent allopolyploidization events involving C. cornuta and two unknown hypothetical diploid species.     

Species composition and distribution patterns of baetid nymphs (Baetidae: Ephemeroptera) in a Japanese stream

Species composition and distributional patterns among nymphs of five baetid genera (Ephemeroptera), Baetis, Tenuibaetis, Labiobaetis, Nigrobaetis and Alainites were investigated in Yura Stream, Kyoto Prefecture. I collected 13 species: B. sahoensis, B. thermicus, B. sp. F, B. sp. J, B. sp. M1, B. sp. S1, T. sp. E, T. sp. H, L. sp. G, N. chocoratus, N. sp. D, N. sp. I and A. yoshinensis, among which B. thermicus, B. sp. S1 and T. sp. E were dominant, whereas B. sahoensis, B. sp. F, B. sp. M1 and N.sp. I were scarce. Based on their longitudinal distribution patterns, the 13 species were classified into upper species, upper-middle species, middle species, middle-lower species and lower species. Baetis thermicusand A. yoshinensis showed long downstream tails. Baetis sp. J and N. sp. D extended their longitudinal distribution upstream in summer. With regard to habitat preference, Alainites and Labiobaetis were restricted to riffle and vegetated zones, respectively. Tenuibaetis consisted of riffle-vegetated zone species, whereas Baetis and Nigrobaetiscontained both riffle species and ubiquitous species. Habitat partitioning (`sumiwake') along the watercourse (macro-sumiwake) was evident in Tenuibaetis, and that between habitat types (micro-sumiwake) in Labiobaetis vs. Baetis (rhodanigroup species) and Labiobaetis vs. Alainites.     

Three new species of <Emphasis Type="Italic">Anthocephalum</Emphasis> Linton, 1890 (Cestoda: Tetraphyllidea) from dasyatid stingrays of the Gulf of California

Three new species of Anthocephalum Linton, 1890 are described from dasyatid stingrays collected in the Gulf of California. Anthocephalum michaeli n. sp. is described from Dasyatis longus (Garman). This species most closely resembles A. alicae Ruhnke, 1994, but differs from this species in proglottid number. A. lukei n. sp. is also described from D. longus. This new species is most similar to A. cairae Ruhnke, 1994, but differs from that species in marginal loculi number and number of proglottids. The third new species, A. currani n. sp., is described from D. brevis (Garman). This species is most similar to A. centrurum (Southwell, 1925) Ruhnke, 1994, but differs from that species in marginal loculi number, number of testes and ovarian length. Phyllobothrium kingae Schmidt, 1978 is also consistent in morphology with species of Anthocephalum and is transferred to this genus, forming the new combination Anthocephalum kingae n. comb. This species most closely resembles A. michaeli n. sp., but differs in testicular shape. This brings the total number of species of Anthocephalum to nine. The transfer of the species Phyllobothrium arctowskii Wojciechowska, 1991, P. georgiense Wojciechowska, 1991, P. rakusai Wojciechowska, 1991 and P. siedleckii Wojciechowska, 1991 to Anthocephalum is not warranted, as these four species lack a posteriorly recurved cirrus-sac and a sinuous vagina, and have vitelline follicles uninterrupted by the ovary. Of the nine known species, all are parasitic in batoid fishes, and six are found in species of Dasyatis Garman. The phylogenetic status of Anthocephalum species in relationship to Rhinebothroides Mayes, Brooks & Thorson, 1981, Pararhineothroides Zamparo, Brooks & Barriga, 1999 and other rhinebothriin taxa is discussed.     

CHROMOSOME NUMBERS IN PERITYLE AND RELATED GENERA (PERITYLANAE-COMPOSITAE)

Chromosome numbers are presented for 28 species of the genus Perityle, one putative inter-sectional hybrid, two species of Amauria, one species of Eutetras, and one species of Pericome. For Perityle, initial counts are recorded for 12 species of sect. Laphamia (n = 16, 17, 18, 36, ca. 102) and 11 species of sect. Perityle (n = 11, 12, 13, 16, 17, 18, 19, 34, 51). Chromosome numbers for the two species of Amauria (n = 18) are first reports for the genus. Including the current information, chromosome numbers have been recorded for 37 of the approximately 50 species recognized for Perityle. At least 24 taxa have numbers of n = 17, suggesting a base chromosome number of x = 17 for Perityle.     

A taxonomic review of ichneumon-flies (Hymenoptera,Ichneumonidae), parasitoids of gall-forming sawflies (Hymenoptera,Tenthredinidae) on Salix. Part II. Review of the Palaearctic species of the genus <Emphasis Type="Italic">Saotis</Emphasis> Förster with description of four new species

This is the second communication on Ichneumonidae, parasitoids of gall-making sawflies on Salix. Twenty-one Palaearctic species of the specialized genus Saotis are revised and classified into 12 species groups. Original data on 29 species of Saotis hosts are given: 18 spp. of Phyllocolpa, 10 Pontania and 1 species of Pontopristia. A key to species and subspecies is given and illustrated by 55 figures. For each species the literature, synonymy, diagnosis, material and hosts are given. Four new species are described: Saotis alpinator sp. n., S. boreator sp. n., S. granulator sp. n. and S. subarctor sp. n.     

Molecular Phylogenetics of the Peromyscus boylii Species Group (Rodentia: Muridae) Based on Mitochondrial Cytochrome b Sequences

Variation in the mitochondrial cytochrome b gene (1143 bp) was examined to estimate the phylogenetic relationships of taxa within the Peromyscus boylii species group. In addition, phylogenetic relationships among the aztecus, boylii, and truei species groups were addressed. Maximum-likelihood, neighbor-joining, and maximum-parsimony (weighted and equally weighted) analyses produced similar topologies with P. boylii, P. beatae, P. simulus, P. stephani, P. madrensis, P. levipes, and three undescribed taxa from western Mexico forming a monophyletic unit. At least two of the undescribed taxa from western Mexico potentially represent species. Members of the P. aztecus species group formed a clade separate from the P. boylii group and should be recognized as a distinct species group. P. sagax, P. polius, and P. pectoralis, formerly placed in the P. boylii species group, generally formed an unresolved polytomy with the P. truei, P. aztecus, and P. boylii species groups. P. attwateri formed a sister taxon relationship with members of the P. truei species group (P. difficilis and P. nasutus) and should be considered a member of this group. Members of the P. truei species group did not form a monophyletic unit, indicating that this species group is not monophyletic and may be composed of two assemblages.     

Chrysophyceae from freshwater localities near Nijmegen,The Netherlands

Scale-bearing Chrysophytes from nine freshwater bodies in the surroundings of Nijmegen, The Netherlands, have been studied using both scanning and transmission electron microscope.A comparison has been made with a previous study in the northern part of The Netherlands by Wujek & van der Veer (1976).This paper deals with three species of Mallomonopsis, twenty-one species and one variety of Mallomonas, four species and three formae of Synura, one species of Chrysosphaerella, two species of Spiniferomonas and three species and one form of Paraphysomonas. One species of Mallomonopsis, twelve species of Mallomonas, one form of Synura, Chrysosphaerella brevispina and one species and one form of Paraphysomonas are for the first time recorded from The Netherlands. One species of Mallomonopsis and one species of Spiniferomonas could not be identified.Some physical and chemical data of the sampling localities are given.