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1.
Abstract Sexually mature male and female Rana esculenta L. were captured in their natural habitat in six phases of the annual cycle. Nuclear volumes in APOA cells were found to fluctuate distinctly in the course of the year. In both sexes nuclear volumes were maximal in the phases preceding the breeding season (IIIrd decade of January, and 1st decade of April), and minimal throughout the phases of active life (IIIrd decade of May, IInd decade of July, and 1st decade of September). No aldehydefuchsin or Gomori‐positive material was found in the APOA perikaryons. 相似文献
2.
ABSTRACTWe investigated the histological structure of the graylag goose (Anser anser) gall bladder. Sections of the gall bladder were stained with hematoxylin and eosin (H & E), Alcian blue (pH 2.5) for acid mucopolysaccharides, Gomori’s method for reticular fibers, Masson’s trichrome, periodic acid-Schiff (PAS) and Verhoeff’s elastin stain. The goose gall bladder was composed of a tunica mucosa, tunica muscularis and tunica adventitia or tunica serosa. The tunica mucosa formed regularly distributed simple isometric folds plus larger, less numerous, branched folds. The luminal surface was lined by tall columnar epithelial cells that stained for both acid and neutral mucopolysaccharides. The epithelial cells formed a discontinuous striated border of interdigitating microvilli on the luminal surface. Neither a lamina muscularis nor goblet cells were observed in the tunica mucosa. Unusual findings included branched mucosal folds, discontinuous microvilli and absence of an outer longitudinal layer in the tunica muscularis. No marked sex-associated differences were found. The general histochemical and histological structures of the graylag goose gall bladder are similar to those of birds such as chukar partridge and quail, but with some unique elements that may reflect differences in organ function. 相似文献
3.
Abstract A new species of the neotropical electric fish genus Compsaraia is described from the western Amazon of Peru and Brazil. Compsaraia samueli is distinguishable from all other apteronotids by sexual dimorphism in which mature males exhibit extreme elongation and slenderness of the snout and jaws. Compsaraia samueli is readily distinguishable from its only congener, C. compsa, by more caudal‐fin rays (17–18 vs. 13–16), a shorter caudal peduncle (mean length 9% vs. 34% body length to end of anal fin), a less tapering body shape in lateral profile (mean ratio of body depth at origins of anal fin and dorsal organ 93% vs. 75%), and a smaller maximum adult body size (230 vs. 305 mm). The genus Compsaraia is readily separated from other apteronotids by a pale antorbital stripe and a pale L‐shaped patch over the supra‐temporal canal. The phylogenetic position of C. samueliis estimated by inclusion in a previously published data matrixof osteological and other morphological characters. Comparisons of the cranial bones in apteronotids shows the derived morphology of C. samueli to be a composite of three developmentally and phylogenetically discrete characters: (1) positive allometric growth before sexual maturity in both sexes of the pre‐orbital region of the neurocranium, (2) positive allometric growth of the (oral) jaws, and (3) secondary sexual dimorphism of snout and jaw morphology. The genus Compsaraia represents one of at least three phylogenetically independent cases of snout elongation and one of at least four cases of jaw elongation within the Apteronotidae. Compsaraia samueli also represents one of at least four cases of secondary sexual dimorphism in snout and jaw length within the Apteronotidae. The phylogenetic distribution of snout and jaw characters within the Apteronotidae suggests the influence of both sexual and trophic functional influences on the evolution of head morphology. 相似文献
4.
The perioperative time course and clinical significance of the chemokine CXCL16 in patients undergoing cardiac surgery
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Daniela Dreymueller Andreas Goetzenich Christoph Emontzpohl Josefin Soppert Andreas Ludwig Christian Stoppe 《Journal of cellular and molecular medicine》2016,20(1):104-115
The chemokine CXCL16 and its receptor CXCR6 have been linked to the pathogenesis of acute and chronic cardiovascular disease. However, data on the clinical significance of CXCL16 in patients undergoing cardiac surgery with acute myocardial ischemia/reperfusion (I/R) are still lacking. Therefore, we determined CXCL16 in the serum of cardiac surgery patients and investigated its kinetics and association with the extent of organ dysfunction. 48 patients underwent conventional cardiac surgery with myocardial I/R and the use of cardiopulmonary bypass (CPB) were consecutively enrolled in the present study. We investigated the peri‐ and post‐operative profile of CXCL16. Clinical relevant data were assessed and documented throughout the entire observation period. To identify the influence of myocardial I/R and CPB on CXCL16 release data were compared to those received from patients that underwent off‐pump procedure. Pre‐operative serum CXCL16 levels were comparable to those obtained from healthy volunteers (1174 ± 55.64 pg/ml versus 1225 ± 70.94). However, CXCL16 levels significantly increased during surgery (1174 ± 55.64 versus 1442 ± 75.42 pg/ml; P = 0.0057) and reached maximum levels 6 hrs after termination of surgery (1174 ± 55.64 versus 1648 ± 74.71 pg/ml; P < 0.001). We revealed a positive correlation between the intraoperative serum levels of CXCL16 and the extent of organ dysfunction (r2 = 0.356; P = 0.031). Patients with high CXCL16 release showed an increased extent of organ dysfunction compared to patients with low CXCL16 release. Our study shows that CXCL16 is released into the circulation as a result of cardiac surgery and that high post‐operative CXCL16 levels are associated with an increased severity of post‐operative organ dysfunctions. 相似文献
5.
MARIJKE DE JONG-BRINK BRENDA VAN DER WAL XAVIER E. OP DE LAAK MARION J.M. BERGAMIN-SASSEN 《Invertebrate reproduction & development.》2013,57(1-3):223-227
Summary Myoblasts, muscle cells with the capacity to divide, have been detected in “Anlagen” of the male copulation organ of Lymnaea stagnalis. They only occur in the apical part of the penis. Here they could be found throughout life. Mitotic activity of these cells can be demonstrated by using an antiserum to a S-phase specific cell cycle marker, PCNA [see, e.g., Baserga (1991)]. The number/percentage of PCNA positive myoblasts is a good parameter for growth of this male copulation organ and hence also for inhibition of its growth and development as occurs in parasitized snails. In transplantation experiments, “Anlagen” of the copulation organ were used from snails 7–9 weeks after being parasitized as they can be excised in this stage and transplanted into either parasitized or nonparasitized snails. These experiments have indicated that humoral, parasitic excretory/secretory factors can be responsible for the inhibition of growth and differentiation of the copulation organ in parasitized snails as reflected by a relatively low number of PCNA positive myoblasts compared to the controls. Data obtained in in vitro experiments showed a significant decrease of the number of myoblasts in “Anlagen” cultured in the presence of parasitic E/S products. The fact that no significant effect was found on the relative low number of PCNA positive myoblasts is discussed. The effect of parasitic E/S products on these myoblasts appeared to be exerted in a direct way, not mediated by CNS-derived factors or by factors from cells in the connective tissue sheath around the CNS. Although it appears possible to use transplantation and/or in vitro culturing of these “Anlagen” as a bioassay for identification of the parasitic factor(s) responsible for the inhibitory effects on myoblasts, the methods are very laborious and do not seem very appropriate for testing many fractions of E/S products. 相似文献
6.
Abstract A very distinct annual cycle of activity, as judged from nuclear volume changes, has been found in glandular cells of the pars tuberalis of the hypophysis and neurons in the nucleus tuberis hypothalami of adult green frogs (Rana esculenta). The changes are very similar at both sites, a single maximum in April and a single minimum in September being observed. Ependymal tanycy tes of the region which was investigated showed only very moderate activity changes. No sex differences were found with regard to the character of the cycle and the amplitude of changes. Only quantitative differences in absolute nuclear volume were found between the two sexes. 相似文献
7.
Birgit Sonnleitner Thomas Schwaha Andreas Wanninger 《Journal of Zoological Systematics and Evolutionary Research》2014,52(1):1-14
Recent molecular data place Phoronida within the protostome superclade Lophotrochozoa, where they have been suggested to form a monophyletic assemblage with Brachiopoda and/or Nemertea. Herein, the anatomy of the nervous system and the structure of the apical organ are described for two larval stages of Phoronis muelleri in order to contribute to the discussion concerning the evolution of lophotrochozoan nervous systems. Specimens were investigated by confocal laser scanning microscopy using antibodies against the serotonin‐like immunoreactive (serotonin‐lir), the FMRF‐amide‐like immunoreactive (FMRFamide‐lir) and the small cardioactive peptide B‐like immunoreactive (small cardioactive peptide B‐lir) compounds of the nervous system. Consistent with larvae of other phoronid species, we found a complex apical organ that consists of numerous serotonin‐lir flask‐shaped cells, additional bi‐ or multipolar serotonin‐lir cells and several FMRFamide‐lir perikarya. A detailed comparison between our results and those of a previous study on the same species shows significant differences in the innervation of the preoral lobe, the tentacles and the telotroch. Our work is the first to prove the presence of small cardioactive peptide B in phoronids. In larvae of P. muelleri, it is expressed in neurites along the margin of the preoral hood, in the mesosome, in the tentacles, in the trunk as well as in the apical organ. A positive signal for this peptide is also known from molluscs, annelids and arthropods, indicating that it was also part of the protostomian groundplan. In contrast to a recent study on another phoronid species, Phoronopsis harmeri, we did not find a ventral neurite bundle in the larval stages investigated herein, thus leaving the question open whether this structure was part of the phoronid groundplan or evolved de novo in P. harmeri. 相似文献
8.
The planulae of almost all investigated cnidarian species possess neuron‐like cells. The distribution of these cells is usually uneven throughout the long axis of the planula. The majority of these cells are located in the anterior half of the planula body. Scyphozoan planulae, as well as anthozoan planulae, have a sensory structure at the anterior pole called an apical organ, which is believed to take part in metamorphosis induction. Hydrozoan planulae also possess sensory cells. It has been previously shown in several cnidarian larvae that their neuronal cells contain the neurotransmitter, serotonin. The present study describes the peculiarities of serotonin‐like immunoreactive cells in Aurelia aurita (Scyphozoa) and Gonothyraea loveni (Hydrozoa) planulae. We show that several cells in the presumptive apical organ of A. aurita are immunoreactive to antibodies against serotonin, while G. loveni planulae have an accumulation of serotonin‐positive cells near the anterior pole. Additional serotonin‐like immunoreactive cells are found in the lateral ectoderm of both planulae. Treatment of A. aurita and G. loveni planulae with serotonin or its blockers show that serotonin is likely involved in the initiation of planula settlement. 相似文献
9.
A. G. Hocken 《New Zealand journal of zoology.》2013,40(4):299-304
Abstract This work presents a database of weights of normal internal organs for the blue penguin, Eudyptula minor. During a necropsy study of 213 adult and fledged immature blue penguins, external body measurements and internal organ weights were recorded. The relationship between organ weights and body weights shows that the liver and abdominal fat pad are most closely correlated with the body mass index, a quantitative indication of adequacy of condition. 相似文献
10.
《Journal of morphology》2017,278(10):1321-1332
The present article is a comparative, structural study of the lung of Polypterus senegalus and Erpetoichthys calabaricus , two species representative of the two genera that constitute the Polypteriformes. The lung of the two species is an asymmetric, bi‐lobed organ that arises from a slit‐like opening in the ventral side of the pharynx. The wall is organized into layers, being thicker in P. senegalus . The inner epithelium contains ciliated and non‐ciliated bands. The latter constitute the respiratory surface and are wider in E. calabaricus . The air‐blood barrier is thin and uniform in P. senegalus and thicker and irregular in E. calabaricus . In the two species, the ciliated areas contain ciliated cells, mucous cells and cells with lamellar bodies. Additionally, P. senegalus contains polymorphous granular cells (PGCs) and neuroendocrine cells (NECs) while E. calabaricus lacks PGCs but shows granular leukocytes and a different type of NEC. Interestingly, ciliated cells and secretory cells show a dual morphology in E. calabaricus indicating the presence of cellular subtypes and suggesting more complex secretory activity. Also in E. calabaricus , cilia show a novel doublet‐membrane interaction that may control the displacement of the microtubule doublets. The subepithelium is a connective layer that appears thicker in P. senegalus and contains, in the two species, fibroblasts and granulocytes. The outer layer contains bundles of richly innervated striated muscle. This layer is likely involved in the control of lung motion. In the two species, smooth muscle cells constitute a limiting layer between the subepithelium and the striated muscle compartment. The role of this layer is unclear. 相似文献
11.
Abstract Pyrocystis lunula, a dinoflagellate lacking periodicity in spontaneous bio‐luminescence, shows a characteristic circadian rhythm of plastid movements which can be monitored photographically in individual cells. The rhythm persists under free‐running conditions in constant dim light (40–50 Ix), but is damped out already in LL of 100 lx. 相似文献
12.
Dr. N. Öztan 《Cell and tissue research》1966,75(1):66-82
Summary The Structure of the hypothalamic neurosecretory cells during their activity in continuous light and dark conditions and depending on the seasonal alteration has been investigated in Zoarces viviparus L. in the level of light and electron microscopy.The depletion of AF- or AT-stainable material, of the elementary neurosecretory granules and the disappearance of smooth-surfaced vesicles occur during April and June. The accumulation of the stanable material as well as the elementary neurosecretory granules and the smooth-surfaced vesicles has been observed in the cell body in late September.An excess increase in number of the smooth-surfaced vesicles of dark-induced animals and light-induced animals kept in a black stained tank is apparent. On the other hand, the disappearance of the elementary granules and the smooth-surfaced vesicles and an enlargement in the reletive nuclear surface of the neurosecretory cells of light-induced animals which were kept in a gray stained tank in April is also evident.Taking into consideration the responsiveness of both the elementary neurosecretory granules and the smooth-surfaced vesicles in relation to the external environment as well as their topographical arrangement in the cell body the possible differences in their origin and function are discussed.Numerous studies indicate that the physical environment is in part responsible for the functional properties of the hypothalamic neurosecretory centers of various vertebrates. In the level of light microscope, it has been repeatedly shown that the neurosecretory cells are activated in the animals subjected to continuous illumination, and in those subjected to long daily photo period (Oksche et al., 1958; Fiske and Greep, 1959; Öztan and Gorbman, 1960; Satyanesan, 1965). The enlargement of the nuclei and the amount of Gomori — or aldehyde fuchsin — stainable material, as well as the enzymatic activity, were used as the criteria of cellular activity in the histological preparation. In the level of electronmicroscope it has been shown that Gomori positive material (Bargmann, 1949) is represented as aggregated elementary neurosecretory granules in the ultrathin sections of neurohypophysis (Bargmann et al,. 1957). Although the fine structure of the hypothalamic neurosecretory cells of various fishes and higher vertebrates have been studied in normal and experimental conditions (Palay, 1960; Lederis, 1962, 1964; Follenius et Porte, 1962; Follenius, 1963; Murakami, 1961–1964; Gansler, 1965; Holmes, 1965) the type of granules and their function still remains as an open question (see Bargmann, 1963; Knowles, 1965).This work was aided by a grant from Nato and the Deutsche Forschungsgemeinschaft.This paper was written as a tribute in honor of the 60th birthday of Prof. Dr. Berta Scharrer. 相似文献
13.
M.C. Guglielmo G. Ricci A. Catizone M. Barberi M. Galdieri M. Stefanini R. Canipari 《Journal of cellular physiology》2011,226(2):520-529
Interactions between theca and granulosa cells of the follicle are critical for the coordination of ovarian follicle development. The cell–cell interactions are mediated through the local production and actions of a variety of factors. The current study is designed to investigate the expression of Hgf and its receptor, c‐Met, in the mouse ovary during in vivo folliculogenesis. We found that Hgf and c‐Met mRNAs were already expressed in 2‐day‐old ovaries, and that, while c‐Met levels remained constant until 22‐day‐old, Hgf levels slightly but not significantly increased with age. The expression of Hgf mRNA in theca/interstitial cells was higher than in granulosa cells in 22‐day‐old ovaries. Immunohistochemistry analysis confirmed the expression pattern demonstrated by RT‐PCR. We investigated the role of hepatocyte growth factor (HGF) at the beginning of mouse folliculogenesis and its possible interaction with kit ligand (KL). Interestingly, both KL and HGF were able to increase the expression of each other, creating a positive feedback loop. In the presence of HGF, we observed an increase of granulosa cell proliferation and an increase in the number of pre‐antral and early antral follicles in ovary organ cultures. We also observed a significant increase in the diameters of follicles in individual follicle cultures. Moreover, HGF stimulated the expression of the FSH receptors, both in the whole ovary and in isolated pre‐antral follicle cultures. Based on the data presented, we concluded that HGF exerts multiple levels of control over follicular cell functions, which collectively enable the progression of follicular development. J. Cell. Physiol. 226: 520–529, 2011. © 2010 Wiley‐Liss, Inc. 相似文献
14.
Summary The mantle epithelium of embryos and early juveniles of the squids Loligo vulgaris and Loligo forbesi and the cuttlefish Sepia officinalis was studied using scanning electron microscopy. In embryos of L. vulgaris and L. forbesi, previously undescribed epidermal structures were found. They are missing in S. officinalis embryos. These so-called “extruding structures” are located near Hoyle's organ and first appear at stage XIII of Naef. At the same embryonic stage, Hoyle's organ starts to differentiate and “uniform-type” ciliated cells become visible in the epidermis of both L. vulgaris and L. forbesi. Directly after hatching the epidermis of the species examined starts to slough off and finally the extruding structures, Hoyle's organ and both types of ciliated cells of the mantle epithelium disappear. The function of the extruding structures remains obscure. 相似文献
15.
16.
C. Kurischko M. B. Schilhabel I. Kunze E. Franzl 《Molecular & general genetics : MGG》1999,262(1):180-188
The MAT
A locus of Yarrowia lipolytica, which was on the basis of its ability to induce sporulation in a diploid B/B strain, represses the mating capacity of this strain. The gene functions required for induction of sporulation and repression
of conjugation could be separated by subcloning. Sequence analysis revealed two ORFs in the MAT
A locus. One of them (MAT
A1) codes for a protein of 119 amino acids which is required to induce sporulation. The other (MAT
A2) codes for a protein of 291 amino acids that is able to repress conjugation. Both genes are oriented divergently from a central
promoter region, which possesses putative TATA and CAAT boxes for both genes. The product of MAT
A1 shows no homology to any known protein and seems to represent a new class of mating-type genes. MAT
A2 contains a HMG box with homology to other mating-type genes. Both MAT
A1 and MAT
A2 are mating-type specific. In cells of both mating types, the regions flanking the MAT
A locus contain sequences with homology to either S. cerevisiae SLA2 and ORF YBB9, respectively. From hybridization and subcloning data we estimate that the MAT
A region is approximately 2 kb long and is present only once in the genome.
Received: 25 January 1999 / Accepted: 16 April 1999 相似文献
17.
The MUC5AC glycoprotein is the primary receptor for Helicobacter pylori in the human stomach 总被引:8,自引:0,他引:8
Van de Bovenkamp JH Mahdavi J Korteland-Van Male AM Büller HA Einerhand AW Borén T Dekker J 《Helicobacter》2003,8(5):521-532
Background and objectives. Helicobacter pylori shows a characteristic tropism for the mucus‐producing gastric epithelium. In infected patients, H. pylori colocalizes in situ with the gastric secretory mucin MUC5AC. The carbohydrate blood‐group antigen Lewis B (LeB) was deemed responsible for the adherence of H. pylori to the gastric surface epithelium. We sought to determine if MUC5AC is the carrier of LeB, and thus if MUC5AC is the underlying gene product functioning as the main receptor for H. pylori in the stomach. Methods. We studied three types of human tissue producing MUC5AC: Barrett's esophagus (BE), normal gastric tissue, and gastric metaplasia of the duodenum (GMD). Tissue sections were immuno‐fluorescently stained for MUC5AC or LeB, and subsequently incubated with one of three strains of Texas red‐labeled H. pylori, one of which was unable to bind to LeB. We determined the colocalization of MUC5AC or LeB with adherent H. pylori. Results. The binding patterns for the two LeB‐binding strains to all tissues were similar, whereas the strain unable to bind to LeB did not bind to any of the tissues. In normal gastric tissue, the LeB‐binding strains always bound to MUC5AC‐ and LeB‐positive epithelial cells. In four nonsecretor patients, colocalization of the LeB‐binding strains was found to MUC5AC‐positive gastric epithelial cells. In BE, the LeB‐binding H. pylori strains colocalized very specifically to MUC5AC‐positive cells. MUC5AC‐producing cells in GMD contained LeB. Yet, LeB‐binding H. pylori not only colocalized to MUC5AC or LeB present in GMD, but also bound to the LeB‐positive brush border of normal duodenal epithelium. Conclusions. Mucin MUC5AC is the most important carrier of the LeB carbohydrate structure in normal gastric tissue and forms the major receptor for H. pylori. 相似文献
18.
19.
Abdellah Amimi Aziza Mouradi Laila Bennasser Thiery Givernaud 《Phycological Research》2007,55(2):143-149
The biology and biochemistry of Gigartina pistillata (Gmelin) Stackhouse collected monthly at Nation Beach (Morocco), was studied during one year. The biological study showed one period of active growth from April to July. The thallus composition was quite stable during the major part of the year. The dry matter was maximum in May and August and minimum in January. The maximum carrageenan content occurred in June and September (about 37%) and the minimum carrageenan content occurred in February (19.0%). The total nitrogen content varied significantly, with a maximum in January (1.98%) and a minimum in August (0.7%). The ash content was significant (23–32%) with a maximum in August and a minimum in May. The carrageenan extracted from natural populations of Gigartina pistillata was a mixture of lambda‐type and kappa‐type carrageenans. The 3,6‐anhydrogalactose varied between 4.5 mol% in June to 25 mol% in February. For industrial applications the extract could be considered as a lambda‐type. The best period for harvest of G. pistillata in Morocco is between July and August when biomass and viscosity are at their maximum. A relationship between the physical characteristics of G. pistillata carrageenans and its seasonal cycle was deduced. 相似文献
20.
In this study, we used a macrophyte model to describe the growth production and the interaction between above‐ and below‐ground organs of Potamogeton pectinatus in Lake Burullus, Egypt. Above‐ and below‐ground biomass of P. pectinatus was sampled on a monthly basis from April to December 2011 at three sites of Lake Burullus. Shoots started to grow in April, reached the maximum biomass in September and then rapidly decreased in October when they moved into the senescence stage. Tubers biomass reduced in August due to the upward translocation to shoots, but sharply increased to the maximum in October by downward translocation from shoots and roots. Potamogeton pectinatus allocated approximately 82.3% of its total biomass to shoots, 15.5% to tubers and 2.2% to roots. 相似文献