Two New Species and Rivision of two Specific names of Euonymus

In this paper,two new species of Euonymus are described from China. They are Euonymus jinfoshanensis Z. M. Gu and Euonymus xylocarpus C. Y.Cheng et Z. M. Gu. And the specific names of two species are revised.     

New Taxa of Epimedium L. from China

Three new species and a new variety, Epimedium coactum, E. coactum var. longtouhum, E. trunctum, E. glandulosopilosum are described in this paper. The morphological characters of trichomes, cuticle of leaf surface and pollen grains of the new species and theirallies were studied under LM and SEM, and shown in plates 1-3.     

Late Pleistocene and Holocene equid remains from Israel

Equid remains–mostly isolated teeth–from archaeological sites in Israel are described. Particular attention is paid to dental enamel fold morphology and criteria are established tor separating several Old World Quaternary equid species. Eauus hydruntinus , which is here considered a zebra rather than an ass, was present until 12,000 bp in northern Israel, while at the same time E-asinus/hemionus inhabited the arid regions in the south. Inirequent remains of E. caballus are also described. By 4000 years ago ass, probably the domestic form, was present in northern Israel.     

中国水云科Ectocarpaceae新记录Ⅱ

继续报道中国水云科新记录7种。毛果水云Ectocarpus dasycarpus,束生水云E. fasciculatus,小水云E. parvus,笔头水云E. penicillatus,群居水云E. socialis,丝状费氏藻Feldmannia filifera,壳状扭线Streblonema evagatnm。     

中国家蚕抗菌肽ABP-CM4在E.coli中的融合表达及活性分析

参照天然抗菌肽CM4(ABP-CM4)氨基酸序列和大肠杆菌偏爱密码子,采用rPCR法获得CM4基因后重组到表达载体pET32a上,在E.coli中融合表达。表达产物以可溶性存在,经Ni2 -NTA琼脂糖亲和层析获得融合蛋白,再经甲酸切割、亲和层析和阳离子交换层析,得到纯化的重组抗菌肽。琼脂糖扩散法和液相测定法证明了纯化的抗菌肽具有抗菌活性。     

A New Taxa of the Genus Epimedium

One new species and one new variety of the genus Epimedium (Berberidaceae) are described from China. They are E. enshiense B. L.Guo et Hsiao and E. platypetalum var. tenuis B. L. Guo et Hsiao.     

正青霉属的两个中国新记录种

分离自广西地区的两个中国新记录种：布氏正青霉（Eupenicilliumbrefeldianum（B．Dodge）Stolk＆Scott）,赭瑰色正青霉（EupenicilliumochrosalmoneumScott＆Stolk）。根据广西的分离物对此两个种作了描述并附线条图。     

兰州北山三种蜥蜴春季食性分析

兰州地区密点麻蜥、丽斑麻蜥和草原沙蜥的春季食物均为动物食物。无论在栖息地重叠地区还是非重叠地区,三种蜥蜴的食物种类在种间均无显著差异（Ｆ＝０．８６１,ｄｆＴ＝２,ｄｆＥ＝３６,Ｐ〉０．０５）。种内不同性别和年龄食性无显著差异。     

兰科火烧兰属Epipactis若干种类的增补与修订

讨论了北美火烧兰 Epipactis gigantea、卵叶火烧兰 E．royleana和大叶火烧兰 E．mairei之间的异同。过去被许多作者认为是北美火烧兰 E．gigantea和卵叶火烧兰 E．royleana的中国植物实际上应是大叶火烧兰 E.mairei。卵叶火烧兰在中国仅局限于西藏东南部。     

火烧兰属中一个种的名称考辨

对Epipactis consimilis、火烧兰 E．helleborine和疏花火烧兰 E．veratrifolia之间的形态作了比较,并对Epipactis consimilis D．Don作了名称考证。结果承认 Epipactis veratrifolia Boiss．et Hohen．为合法名称,以取代早先为Rolfe(1903)所接受、近来在《中国植物志》第17卷(1999)中使用的Epipactis consimilis D．Don。     

不同生长年限巨尾桉叶挥发性成分GC-MS分析

为了分析不同年限巨尾桉叶挥发油的化学成分及生长年限对化学成分组成的影响,采用水蒸气蒸馏法,分别提取2006～2010年种植的巨尾桉叶片中的挥发性成分,并用气相色谱质谱联用技术分析鉴定其化学成分。结果表明:不同生长年限的巨尾桉叶片挥发油产量在0.55%～1.35%之间,随生长年限的增加而升高。利用GC-MS共鉴定出63个化合物,占总挥发油的93.00%～97.07%。其中相对含量较高的物质为:1,8-桉叶油素(55.72%～63.47%)、α-松油醇(9.78%～14.15%)、乙酸松油酯(4.96%～7.06%)、α-蒎烯(0.99%～4.31%)、龙脑(2.40%～4.89%)。由于生长年限的不同,挥发油中物质的组成及相对含量均存在差异。     

Response dynamics of 26-, 34-, 39-, 54-, and 80-kDa proteases in induced cultures of recombinant Escherichia coli

Several researchers have demonstrated that the presence of a heterologous protein in recombinant Escherichia coli elicits a response similar to the heat-shock response, which includes enhanced protease expression. The present work detects, quantifies, and characterizes intracellular protease activity in E. coli that are "shocked" by the induction of a recombinant protein, CAT, which is an endogenous protein in some E. coli strains. A novel, sodium dodecyl sulfate gelatin poly-acrylamide gel electrophoresis (SDS-GPAGE) method is used to detect, quantify, and characterize the presence of these proteases. A hypothesis is proposed which links the amplified protease activity to a temporary depletion of specific amino acid pools, and a stringent-like stress response. (c) 1993 John Wiley & Sons, Inc.     

The expression of an R3 lipopolysaccharide-core by pathotypes of Escherichia coli

AIMS: To investigate the incidence of an R3 lipopolysaccharide (LPS)-core amplicon in a range of pathotypes of Escherichia coli, including Verocytotoxin-producing E. coli (VTEC), enteroaggregative E. coli (EAggEC) and enteropathogenic E. coli (EPEC). METHODS AND RESULTS: A total of 100 strains of E. coli belonging to a range of pathotypes, including 41 strains of VTEC, were screened for the genes encoding the R3 LPS-core using PCR. Fifty-four per cent produced an amplicon with the R3 primer set. Of the 41 VTEC, 66% had an R3 LPS-core with a PCR product being observed with all strains belonging to serotypes O26:H11, O111ac:H- and O145:H25. However, 46% of enteroaggregative E. coli and 50% of enteropathogenic E. coli were also shown to have an R3 LPS-core structure. CONCLUSIONS: Strains with an R3 LPS-core are widely distributed within the species E. coli. SIGNIFICANCE AND IMPACT OF THE STUDY: Strains of E. coli with an R3 LPS-core structure appear not to be associated with a specific pathotype.     

长距美冠兰及其近缘种的研究

讨论了长距美冠兰Eulophia dabia及其近缘种的分类与命名问题。Eulophia dabia的基名Bletia Dabia乃是D．Don(1825)合法发表的最早名称,尽管D．Don的“Dabia”很可能是在袭用Hamilton的“dubia”时拼写上的笔误。为了避免进一步的混乱,明智的做法是接受Don拼写的加词,而避开其语源上的麻烦。在分类方面,可以接受Hooker(1890)将Eulophia campestris Lindl．、E．rupestris Lindl、E．ramentacea Lindl．与Bletia Dabia D．Don：予以合并的观点,以及Deva&;Naithani(1986)将Eulophia hormusjii Duthie并入 E．dabia的做法。此外,本文还将Eulophia turkestanica (Litw．)Schltr．和E. faberi Rolfe也归并入 Eulophiadabia。因此,长距美冠兰是一个广布种,白土库曼东部经塔吉克、阿富汗东部、巴基斯坦、克什米尔地区,印度北部、尼泊尔、锡金、不丹、缅甸北部直到中国(云南西南部、四川中部到东部、贵州西南部和江苏),海拔在400~2300 m之间。     

Eimeria and Klossia spp. (Protozoa: Sporozoa) from Wild Mammals in Borneo*

SYNOPSIS. During a survey of parasites of Sabah mammals, coccidia oocysts were found in 9 of 22 host species examined. Sporulated oocysts of Klossia sp. from Rattus whiteheadi are described. New geographic records are reported for Eimeria callosciuri, Eimeria sabani, and Eimeria tupaiae. New host and geographic records are reported for Eimeria separata from Rattus cremoriventer, for E. tupaiae and Eimeria ferruginea from Tupaia tana, for Eimeria nieschulzi from Rattus muelleri, and for E. sabani and Klossia sp. from R. whiteheadi.     

Occurrence of aminoacyl-tRNA synthetase complexes in quiescent wheat germ

The cellular distribution of arginyl-tRNA, leucyl-tRNA, methionyl-tRNA and phenylalanyl-tRNA synthetases from quiescent wheat germ shows that these enzymes participate to various high molecular weight complexes from 3.10⁶ to 3.10⁵. Two types of interactions were discriminated by salt washing. The greater part of these complexes are weakly bound and destroyed by conventional enzymatic extraction procedures which release the free enzymes. Sedimentation constants of complexes show that they have a common size at 18–20S, but higher sedimentation constants were also observed (? 40–60 S). Investigations by gel filtration lead to evidence that the complexes contain a little amount of conjugates.     

Infection by microsporidia disrupts the host cell cycle

Microsporidia of the genus Encephalitozoon infect mammalian cells and have become a source of morbidity and mortality in immunocompromised humans. Encephalitozoon microsporidia develop and mature within parasitophorous vacuoles, enlarging the vacuole over time until it eventually occupies most of the cytoplasm of the host cell. The ability of the host cell to accommodate such a large burden for several days suggests that the parasite subverts normal host cell processes to ensure optimal environmental conditions for its growth and development. Since this environment would be threatened if cell division of the host cell occurred, we have formulated the hypothesis that infection with Encephalitozoon microsporidia induces an arrest in the cell cycle of the host cell. In support of this hypothesis, we have found that mitotic index and DNA duplication are reduced in infected cells as compared to uninfected cells. The number of host cell nuclei in S phase is increased. The levels of cyclin D1 and the percentage of cells in G1 are reduced; however, the levels of cyclin B1 are elevated even though the percentage of cells in G2/M is decreased. These results suggest that host cells infected with Encephalitozoon microsporidia are blocked at multiple points in the cell cycle.