共查询到20条相似文献,搜索用时 21 毫秒
1.
Peter W Thomas Sarah Thomas Paula Kersten Rosemary Jones Alison Nock Vicky Slingsby Colin Green Roger Baker Kate Galvin Charles Hillier 《BMC neurology》2010,10(1):43
Background
Fatigue is one of the most commonly reported and debilitating symptoms of multiple sclerosis (MS); approximately two-thirds of people with MS consider it to be one of their three most troubling symptoms. It may limit or prevent participation in everyday activities, work, leisure, and social pursuits, reduce psychological well-being and is one of the key precipitants of early retirement. Energy effectiveness approaches have been shown to be effective in reducing MS-fatigue, increasing self-efficacy and improving quality of life. Cognitive behavioural approaches have been found to be effective for managing fatigue in other conditions, such as chronic fatigue syndrome, and more recently, in MS. The aim of this pragmatic trial is to evaluate the clinical and cost-effectiveness of a recently developed group-based fatigue management intervention (that blends cognitive behavioural and energy effectiveness approaches) compared with current local practice. 相似文献2.
Background
This paper discusses the problem of automated annotation. It is a continuation of the previous work on the A4-algorithm (Adaptive algorithm of automated annotation) developed by Leontovich and others. 相似文献3.
Jian Guo Andrew V Schally Marta Zarandi Jozsef Varga Peter CK Leung 《Reproductive biology and endocrinology : RB&E》2010,8(1):54
Background
Antagonists of growth hormone-releasing hormone (GHRH) are being developed for the treatment of various human cancers. 相似文献4.
5.
Determination of Tanshinones in Danshen (Salvia miltiorrhiza) by High‐Performance Liquid Chromatography with Fluorescence Detection after pre‐Column Derivatisation
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Naoya Kishikawa Akiko Yamanouchi Mahmoud Hamed El‐Maghrabey Kaname Ohyama Naotaka Kuroda 《Phytochemical analysis : PCA》2018,29(1):112-117
Introduction
Tanshinones are a major class of bioactive ingredients in the traditional herbal medicines, Danshen (Salvia miltiorrhiza). A sensitive and reliable determination method for tanshinones is useful to ensure the quality of Danshen.Objective
To develop a sensitive and selective analytical method for tanshinones by high‐performance liquid chromatography (HPLC) with fluorescence detection after pre‐column derivatisation.Methodology
The proposed method depends on derivatisation reaction of tanshinones with 4‐carbomethoxybenzaldehyde and ammonium acetate forming intensely fluorescent imidazole derivative.Results
The proposed method provided excellent sensitivity with the detection limits of 3.3 nM (66 fmol/injection), 3.2 nM (64 fmol/injection) and 2.0 nM (40 fmol/injection) for cryptotanshinone, tanshinone I and tanshinone IIA, respectively, without the necessity of complicated instrumentations. The developed method is successfully applied to quantify the contents of tanshinones in Danshen.Conclusion
The developed method is the first analytical method for tanshinones by fluorescence detection. Since the derivatisation reaction is selective for the o‐quinone structure of tanshinone, the developed method will become a suitable mean for the discovering of tanshinone type diterpenoids from herbal samples. Copyright © 2017 John Wiley & Sons, Ltd. 相似文献6.
Background
Sexually deceptive orchids of the genus Ophrys attract their pollinators, male insects, on a highly specific basis through the emission of odour blends that mimic the female sex pheromone of the targeted species. In this study, we have investigated a contact site between Ophrys arachnitiformis and O. lupercalis, two sympatric orchid species that are usually reproductively isolated via the exploitation of different pollinator "niches", but occasionally hybridise despite their apparent combination of ethological and mechanical isolation barriers. In particular, we have investigated the extent to which these Ophrys hybrids generate "emergent" combinations (i.e. novel and unpredictable from the parents' phenotypes) of floral traits, and how these phenotypic novelties, particularly the odour blends emitted by the flower, could facilitate the invasion of a novel pollinator "niche" and induce the rapid formation of reproductive isolation, a prerequisite for adaptive evolutionary divergence. 相似文献7.
Bernd Liedert Ulf Forssmann Peter Wolna Michaela Golob Andreas Kovar 《BMC clinical pharmacology》2010,10(1):14
Background
Somatropin is recombinant human growth hormone (GH) used for the treatment of growth failure in children and GH deficiency in adults. Two concentrations of a liquid formulation have been developed: 5.83 and 8.0 mg/mL. This trial compared the pharmacokinetics (PK), safety and tolerability of these two liquid concentrations against the freeze-dried (FD) formulation in healthy volunteers. 相似文献8.
Past vegetation dynamics in the Yellowstone region highlight the vulnerability of mountain systems to climate change
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Virginia Iglesias Cathy Whitlock Teresa R. Krause Richard G. Baker 《Journal of Biogeography》2018,45(8):1768-1780
Aim
Reconstruct the long‐term ecosystem dynamics of the region across an elevational gradient as they relate to climate and local controls. In particular, we (1) describe the dominant conifers' history; (2) assess changes in vegetation composition and distribution; and (3) note periods of abrupt change versus stability as means of better understanding vegetation responses to environmental variability.Location
Greater Yellowstone Ecosystem (GYE; USA).Time period
16.5 ka bp ‐present.Major taxa studied
Juniperus, Picea, Abies, Pinus, Pseudotsuga.Methods
The vegetation reconstruction was developed from 15 pollen records. Results were interpreted based on modern pollen–vegetation relationships estimated from a suite of regression‐based approaches.Results
Calibrated pollen data suggest that late‐glacial vegetation, dominated by shrubs and Juniperus, lacks a modern counterpart in the area. Picea, Abies and Pinus expanded at 16 ka bp in association with postglacial warming and co‐occurred in mixed‐conifer parkland/forest after 12 ka bp . This association along with Pinus contorta forest, which was present after 9 ka bp , has persisted with little change at middle and high elevations to the present day. This stability contrasts with the dynamic history of plant communities at low elevations, where shifts between parkland, steppe and forest over the last 8,000 years were likely driven by variations in effective moisture and fire.Main conclusions
The postglacial vegetation history of the GYE highlights the dynamic nature of mountain ecosystems and informs on their vulnerability to future climate change: (1) most of the conifers have been present in the area for >12,000 years and survived climate change by adjusting their elevational ranges; (2) some plant associations have exhibited stability over millennia as a result of nonclimatic controls; and (3) present‐day forest cover is elevationally more compressed than at any time in history, probably due to the legacy of the Medieval Climate Anomaly and the Little Ice Age. 相似文献9.
Maria H Nilsson Gun-Marie Hariz Klas Wictorin Michael Miller Lars Forsgren Peter Hagell 《BMC neurology》2010,10(1):85
Background
The Freezing of Gait Questionnaire (FOGQ) was developed in response to the difficulties of observing and quantifying freezing of gait (FOG) clinically as well as in laboratory settings. However, as the FOGQ is a clinician-administered patient-reported rating scale it cannot be used in postal surveys. Here we report the development and measurement properties of a self-administered version of the FOGQ (FOGQsa). 相似文献10.
Jose L Izquierdo-García Ignacio Rodríguez Angelos Kyriazis Palmira Villa Pilar Barreiro Manuel Desco Jesús Ruiz-Cabello 《BMC bioinformatics》2009,10(1):363
Background
Analysis of the plethora of metabolites found in the NMR spectra of biological fluids or tissues requires data complexity to be simplified. We present a graphical user interface (GUI) for NMR-based metabonomic analysis. The "Metabonomic Package" has been developed for metabonomics research as open-source software and uses the R statistical libraries. 相似文献11.
Chih-Ming Lu Yu-Jen Wu Cheng-Chi Chen Jue-Liang Hsu Jiing-Chuan Chen Jeff Yi-Fu Chen Chun-Hsiung Huang Ying-Chin Ko 《Proteome science》2011,9(1):17
Background
Low-abundance proteins are difficultly observed on the two-dimensional gel electrophoresis (2-DE) maps of urine proteome, because they are usually obscured by high-abundance proteins such as albumin and immunoglobulin. In this study, a novel fractionation method was developed for enriching low-abundance proteins by removing high-abundance proteins and progressive elution with salts of various concentrations. 相似文献12.
Fátima Al-Shahrour Leonardo Arbiza Hernán Dopazo Jaime Huerta-Cepas Pablo Mínguez David Montaner Joaquín Dopazo 《BMC bioinformatics》2007,8(1):114
Background
With the popularisation of high-throughput techniques, the need for procedures that help in the biological interpretation of results has increased enormously. Recently, new procedures inspired in systems biology criteria have started to be developed. 相似文献13.
Steffen Klamt Julio Saez-Rodriguez Jonathan A Lindquist Luca Simeoni Ernst D Gilles 《BMC bioinformatics》2006,7(1):56-26
Background
Structural analysis of cellular interaction networks contributes to a deeper understanding of network-wide interdependencies, causal relationships, and basic functional capabilities. While the structural analysis of metabolic networks is a well-established field, similar methodologies have been scarcely developed and applied to signaling and regulatory networks. 相似文献14.
Background
A method for inhibiting the expression of particular genes using external guide sequences (EGSs) has been developed in bacteria, mammalian cells and maize cells. 相似文献15.
Francesca Ceroni Simone Furini Emanuele Giordano Silvio Cavalcanti 《Journal of biological engineering》2010,4(1):14
Background
Most of synthetic circuits developed so far have been designed by an ad hoc approach, using a small number of components (i.e. LacI, TetR) and a trial and error strategy. We are at the point where an increasing number of modular, inter-changeable and well-characterized components is needed to expand the construction of synthetic devices and to allow a rational approach to the design. 相似文献16.
Background
Identifying pockets on protein surfaces is of great importance for many structure-based drug design applications and protein-ligand docking algorithms. Over the last ten years, many geometric methods for the prediction of ligand-binding sites have been developed. 相似文献17.
Koga H Shimaya M Muneta T Nimura A Morito T Hayashi M Suzuki S Ju YJ Mochizuki T Sekiya I 《Arthritis research & therapy》2008,10(4):R84
Introduction
Current cell therapy for cartilage regeneration requires invasive procedures, periosteal coverage and scaffold use. We have developed a novel transplantation method with synovial mesenchymal stem cells (MSCs) to adhere to the cartilage defect. 相似文献18.
Background
Gecko (Gene Expression: Computation and Knowledge Organization) is a complete, high-capacity centralized gene expression analysis system, developed in response to the needs of a distributed user community. 相似文献19.
Background
Sucrose density gradient centrifugation and cross-flow filtration methods have been developed and standardised for the safe and reproducible production of inactivated arbovirus antigens which are appropriate for use in diagnostic serological applications. 相似文献20.