小煤炱科子囊壳超微结构研究*

扫描电镜对小煤炱科Meliolaceae的美座附丝壳 Appendiculella calostroma (Desm．) Hoehnel和莫勒针壳炱 Irenopsis molleriana(Wint．)Stev．的子囊壳进行观察的结果,发现它们的子囊壳都有一个小孔口,孔口周同有2～4层小圆柱形至椭圆形细胞;它们的子囊壳表面细胞呈疣状或乳突状,具纵向沟纹。其中美座附丝壳还有具横条纹的蠕虫状附属物,莫勒针壳炱有光滑的子囊壳刚毛。子囊壳表面孔Izl的发现为小煤炱目Meliolales的确立提供了直接的证据。     

小煤炱科子囊壳超微结构研究

用扫描电镜对小煤炱科的美座附丝壳和莫勒针壳炱。的子囊壳进行观察的结果,发现它们的子囊壳都有一个小孔口。孔口周围有２￣４层小圆柱形至椭圆形细胞;它们的子囊壳表面细胞呈疣状或乳突状,具纵向沟纹,其中美座附丝壳还有具横条纹的蠕虫状附属物,莫勒针壳炱有光滑的子囊壳刚毛,子囊壳表面孔口的发现为小煤炱目的确立提供了直接的证据。     

中国的小煤炱科IV.

本文报告２７个分类单位,它们分别属于小煤炱属,小光壳炱属,附丝壳属和针壳炱属;联昌小煤炱,帽柱木生小煤炱水锦树生变种和拟石珠小光壳炱假木荷变种为新种和新变种。非洲小煤炱,蒲葵小煤炱,坦卡小煤炱为我国新记录。     

中国的小煤炱科Ⅳ.

本文报告27个分类单位,它们分别属于小煤炱属,小光壳炱属,附丝壳属和针壳炱属;联昌小煤炱(Meliola lianchangensis Jiang)、帽柱木生小煤炱水锦树生变种(Meliola mitragynicola Deight.var.wendlandiicola Jiang)和拟石珠小光壳炱假木荷变种(Asteridiella gaylussaciae Hansf.var craibiodendri Jiang)为新种和新变种。*非洲小煤炱,*蒲葵小煤炱,*坦卡小煤炱为我国新记录。     

中国的小煤炱科IV．

本文报告27个分类单位,它们分别属于小煤炱属,小光壳炱属,附丝壳属和针壳炱属;联昌小煤炱(Melioal lianchangensis Jiang)、帽柱木生小煤炱水锦树生变种(Meliola mitra-gynicola Deight. Var. wendlandiicola Jiang)和拟石珠小光壳炱假木荷变种(Asteridiellagaylussaciae Hansf. Var craibiodendri Jiang)为新种和新变种。非洲小煤炱,蒲葵小煤炱,坦卡小煤炱为我国新记录。     

海南省的小煤炱菌(英文)

本文根据Hansford(1961)的分类系统和鉴定方法,采集、鉴定和整理了海南省的小煤炱菌共4属73种;附丝壳属(Appendiculella)2种,小光壳炱属(Asteridiella)10种,针壳炱属(Irenopsis)3种以及小煤炱属(Meliola)58种,其中包括2个新种和29个在中国分布的新记录。新种是:黄牛木生小光壳炱Asteridiella cratoxylonicola Y.X.Hu海南小煤炱Meliola hainanensis Y.X.Hu新种有英文、拉丁文描述和显微结构图。模式标本藏于广东省微生物研究所。     

海南省的小煤炱菌*

本文根据Hansford(1961)的分类系统和鉴定方法,采集、鉴定和整理了海南省的小煤炱菌共4属73种;附丝壳属(Appendiculella)2种,小光壳炱属(Asteridiella)10种,针壳炱属(Irenopsis)3种以及小煤炱属(Meliola)58种,其中包括2个新种和29个在中国分布的新记录。新种是:黄牛木生小光壳炱Asteridiella cratoxylonicola Y.X.Hu海南小煤炱Meliola hainanensis Y.X.Hu新种有英文、拉丁文描述和显微结构图。模式标本藏于广东省微生物研究所。     

裸子植物上的三个小煤炱科新种

本文报道寄生在裸子植物上的三个小煤炱科新种。它们是(1)杉科上的杉木附丝壳Appendiculella cunninghamiae Y.X.Hu et B.Song,杉科是小煤炱科寄主的世界新记录;(2)罗汉松科上的竹柏生小煤炱Meliola podocarpicola Y.X.Hu et B.Song;(3)买麻藤科上的买麻藤生小煤炱Meliola gneticola Y.X.Hu。其形态特征有拉丁文和汉文描述,并附有形态图。模式标本存放在广东省微生物研究所。     

旌节花科植物上的二个小煤炱科新种

本文报道了寄生在旌节花科植物上的二个小煤炱科新种,它们是（１）旌节花附丝壳。（２）旌节花小光壳炱。旌节花科分布于东亚,是小煤炱菌寄主的世界新记录。新种有拉丁文和汉文描述。模型标本存放在广东省微生物研究所。     

旌节花科植物上的二个小煤炱科新种

本文报道了寄生在旌节花科植物上的二个小煤炱科新种。它们是(1)旌节花附丝壳(Appendiculella stachyuri Y.X.Hu et B.Song sp.nov.);(2)旌节花小光壳炱(Asteridie-lla stachyuri Y.S.Ouyang et B.Song sp.nov.)。旌节花科分布于东亚,是小煤炱菌寄主的世界新记录。新种有拉丁文和汉文描述。模式标本存放在广东省微生物研究所(GDIM)。     

A new species of the genus Laboulbenia (Laboulbeniales) on Ophionea indica (Coleoptera, Carabidae, Odacanthini) from Taiwan

Laboulbenia ophioneae is described as a new species with illustrations. It is closely related to Laboulbenia celestialis and Laboulbenia asiatica because of some similarities in the morphology of appendages and perithecia. The present species from the latter two species can be distinguished by the shorter, inflated perithecia, the shorter receptacles, and the appendages consisting of more or less darkly colored, broader branches. This new species was found on elytra of Ophionea indica from Taiwan, which has been also known as a host of Laboulbenia polymorpha. A mature thallus noticed by Terada (2004) on the slide 673b (M. Ishikawa collection) and a young thallus illustrated by Sugiyama (1978, fig. 1-G as L. polymorpha) on 673d (M. Ishikawa collection) are both identified as L. ophioneae.     

Neurospora discreta,a new heterothallic species defined by its crossing behavior

The species is described and namedNeurospora discreta sp. nov. because of its stringent reproductive isolation. Isolates collected from burned vegetation at a single site near Kirbyville, Texas, include both mating types (Aanda). Experimental criteria based on cross-fertility were used for assigning species status. Crosses between isolates of opposite mating type are highly fertile, producing abundant eightspored asci. In contrast, when the Kirbyville strains are crossed to sexually compatible speciestester strains representingN. crassa, N. intermedia, N. sitophila, andN. tetrasperma, perithecia are rudimentary and no ascospores are produced. The haploid chromosome number is 7. Chromosomes at pachytene resemble those of otherNeurospora species. Biotin is required. Linear growth is slower than for other heterothallic species. When A and a strains from Kirbyville grow toward one another and intersect on crossing medium, there is no barrage. A single homogeneous band of perithecia is formed where they meet, indicating that opposite mating types are vegetatively compatible. The Kirbyville population differs from other heterothallicNeurospora species in ascospore morphology and vegetative traits. Ascospores from Kirbyville parents are larger, and the ribs between confluent parallel grooves are ornamented with dot-like pits. Vegetative cultures from Kirbyville are yellowish rather than orange, and large empty barren protoperithecia or false perithecia are produced abundantly in unfertilized haploid cultures. Isolates from two otherN. discreta populations resemble otherNeurospora species more closely with respect to these morphological traits but are clearly conspecific with the Kirbyville strains on the basis of fertility in crosses.     

Three new <Emphasis Type="Italic">Ophiostoma</Emphasis> species with <Emphasis Type="Italic">Pesotum</Emphasis> anamorphs associated with bark beetles infesting <Emphasis Type="Italic">Abies</Emphasis> species in Nikko,Japan

Three species of Ophiostoma possessing Pesotum anamorphs isolated from bark beetles and their galleries infesting Abies species in Nikko, Japan, are described as new species. Ophiostoma nikkoense is characterized by brush-shaped synnemata producing long septate clavate conidia, perithecia with neck, and allantoid ascospores. Ophiostoma microcarpum has smaller perithecia with hyphoid ostiolar hyphae on the neck, and the ascospores are cylindrical or ossiform in side and face views. Ophiostoma abieticola has perithecia without ostiolar hyphae on the neck and produces orange-section-shaped or reniform ascospores.Contribution no. 187, Laboratory of Plant Parasitic Mycology, Institute of Agriculture and Forestry, University of Tsukuba     

Relative Heterothallism and Production of Hybrid Perithecia by Auxotrophic Mutants of Glomerella cingulata from Apple

Glomerella cmgulata is a homothallic species but produces a ridge of fertile perithecia at a frontier between certain wild-type strains on agar. To account for the presence or absence of perithecia earlier workers suggested that alleles at A and B loci control the formation of perithecia at mycelial frontiers in + and – strains. We propose that G. cingulata actually demonstrates “relative heterothallism”. Of 7 induced nutritionally deficient mutants (auxotrophs) in 2 wild-type strains from apple, only one methionine (met-1) and one arginine (arg) mutant in only one wild-type strain gave a heavy ridge of perithecia at their junctures. Neither the met-l nor arg mutations have been identified as those in the A or B locus. The perithecia were either homozygous (selfs) for met-1 or arg, or heterozygous (hybrids). Paired met-1 and arg segregants from hybrid perirhecia as well as diauxotrophic strains from met-l or arg mutants also gave hybrids of selfs. Specific nutritional deficiencies in certain wild-type strains which can direct sexuality are not yet known. Genetic studies are now feasible in G. cingulata to define enzymatic factors responsible for pathogenicity.     

黑腐皮壳属一新种

本文报道发生于我国沈阳地区落叶松枝上的一个新种——大孢黑腐皮壳(Valsa macro-spora sp.nov.)。新种的子囊孢子在15μm以上,属于大型种。其形态特征有汉文和拉丁文描述及其培养性状的记述。     

Stromatonectria gen. nov. and notes on Myrmaeciella

Myrmaeciella caraganae was recollected in and around Vienna, Austria and found to be morphologically different generically from the type species of Myrmaeciella, M. endoleuca. We redescribe M. caraganae in the new genus Stromatonectria. Phylogenetic analyses of LSU sequences place the genus in the Bionectriaceae, Hypocreales. S. caraganae occurs on branches of Caragana spp., Colutea arborescens and Laburnum anagyroides of the Fabaceae. It is characterized by spheroid perithecia partly or entirely immersed in a Hypocrea-like stroma, a Nectria-like centrum and bicellular hyaline ascospores. Conidia of S. caraganae are produced in compound pycnidia that are formed prior to or in association with perithecia. Sporodochia but no pycnidia are formed in culture. We discuss the genus Myrmaeciella and compare S. caraganae with species of the Nectriaceae, including Nectria balansae, N. eustromatica and N. paraguayensis.     

Molecular phylogeny of the entomopathogenic fungi of the genus Cordyceps (Ascomycota: Clavicipitaceae) and its evolutionary implications

Abstract Cordyceps is an endoparasite ascomycetous genus containing approximately 450 species with a diversity of insect hosts, traditionally included in the family Clavicipitaceae of Ascomycota. Establishing the relationships among species with a varied range of morphologies and hosts is of importance to our understanding of the phylogeny and co‐evolution of parasites and hosts in entomopathogenic ascomycetes. To this end, we used a combination of molecular index and morphological characters from 40 representative species to carry out comprehensive molecular phylogenetic analyses. Based on the phylogenetic tree, we used the program DISCRETE for inferring the rates of evolution and finding ancestral states of morphological character. The phylogenetic analyses revealed two important points. (i) Types of perithecia attached to stroma reflected an evolutionary trend in Cordyceps. The vertically immersed perithecia form was the ancestral state, superficial and obliquely immersed perithecia were derived characters, obliquely immersed was irreversible. Species with obliquely immersed perithecia were in a closely related group and were the derived group. (ii) A strong correlation between fungal relatedness and the microhabitat supported the hypothesis that the host jumps through commingling in soil microhabitats. Based on the results of these analyses, host switching explains the diversity of entomopathogenic fungi of the genus Cordyceps.     

Four new species and a new Chinese record of the nectrioid fungi

Four new species belonging to Bionectria, Calonectria, Haematonectria and Neonectria on plant substrates collected from nature reserves in southern and central China are described. Bionectria truncata has smooth perithecia of a flattened to shallow discoid apex, clavate asci with an apical ring, and ellipsoid, smooth to spinulose ascospores. Calonectria dicephalospora is characterized by pyriform perithecia with a warted surface, clavate asci with a simple apex and long, narrow stalk, and fusoid ascospores with a cap-like appendage at each end. Haematonectria lushanensis possesses warted perithecia which are laterally collapsing when dry, cylindrical asci with a simple apex, and ellipsoid, spinulose ascospores. Neonectria dinghushanica is distinguishable by subglobose perithecia with a warted surface, clavate asci, and striate ascospores. Morphological features of these new species are described comprehensively and compared with their related fungi. Neonectria castaneicola is recorded as new to China.     

New species from the Fusarium solani species complex derived from perithecia and soil in the old World tropics

A large collection of strains belonging to the Fusarium solani species complex (FSSC) was isolated from soil and perithecia in primary forests in Sri Lanka (from fallen tree bark) and tropical Australia (Queensland, from fallen tree fruits and nuts). Portions of the translation elongation factor 1-alpha (tef1) gene, the nuclear large subunit (NLSU) and internal transcribed spacer regions (ITS) of the nuclear ribosomal RNA genes were sequenced in 52 isolates from soil and perithecia. The FSSC was divided previously into three clades with some biogeographic structure, termed Clades 1, 2 and 3. All Sri Lankan and Australian soil isolates were found to be members of Clade 3, most grouping with the cosmopolitan soil-associated species F. falciforme. All but two Sri Lankan perithecial isolates were associated with a set of five divergent phylogenetic lineages that were associated with Clade 2. Australian perithecial isolates resided in a subclade of Clade 3 where most of the previously defined mating populations of the FSSC reside. Isolates from perithecia and those cultured from soil were always members of different species lineages, even when derived from proximal locations. The previous biogeographic assignment of Clade 2 to South America is now expanded to the worldwide tropics. Sri Lanka appears to be an important center of diversity for the FSSC. Nectria haematococca is epitypified with a collection from the type locality in Sri Lanka; its anamorph is described as a new species, Fusarium haematococcum. Neocosmospora E.F. Smith is adopted as the correct genus for Nectria haematococca. These new species are described: F. kurunegalense/Neo. kurunegalensis, F. rectiphorus/Neo. rectiphora/, F. mahasenii/Neo. mahasenii/, F. kelerajum/Neo. keleraja.