Influence of myocardial isomyosins on cardiac performance and oxygen consumption

There was 21% isomyosin V1 in the 12 month SHR (Spontaneous Hypertensive Rat) and 70% isomyosin V1 in the 6 month WKY (Wystar-Kyoto), nevertheless there was no difference in maximum developed pressure nor maximum dP/dt in the isovolumically beating hearts of the two sets of animals. [Hearts were perfused with hypercalcemic perfusate in the presence of isoproterenol (10(-7)M)]. There was, however, a a 32% reduction in oxygen consumption per gram of dry weight per beat in the 12 month SHR as compared to the 6 mo WKY. Associated with a shift towards isomyosins V2 and V3 in the 6 and 12 month WKY and SHR there was no corresponding change in maximum dP/dt nor developed pressure, but there was a conservation in oxygen consumption.     

Bentazon influence on selected metabolic processes of isolated bean leaf cells

Time- and concentration-course studies were conducted to determine the effect of bentazon [3-isopropyl-1H-2,l,3,-benzothiadiazin-4(3H)-one 2,2-dioxide] on photosynthesis, RNA synthesis, protein synthesis, and lipid synthesis using enzymatically isolated leaf cells of red kidney bean (Phaseolus vulgaris L.). Photosynthesis and RNA synthesis were inhibited about 75% at 1 μM bentazon at the 30 min treatment period. This was the lowest concentration and shortest time that significantly inhibited any of these four processes. The degree of inhibition of photosynthesis was greater than the degree of inhibition of RNA synthesis at higher concentrations and/or longer time periods. At 10 μM bentazon, protein synthesis and lipid synthesis were also inhibited. Lipid synthesis was stimulated at 0.1 and 1 μM at 120 min.     

Butachlor influence on selected metabolic processes of plant cells and tissues

Time- and concentration-course studies were conducted to determine the effects of butachlor (N-[butoxymethyl]-2-chloro-2,6-diethylacetanilide) on photosynthesis, protein synthesis, RNA synthesis, and lipid synthesis using isolated leaf cells of red kidney bean (Phaseolus vulgaris L.). At the 2-h incubation period, butachlor inhibited photosynthesis, protein synthesis, RNA synthesis, and lipid synthesis 99, 99, 96, and 81% respectively at 100 M, and 0, 19, 17, and 40% respectively at 10 M. At 100 M and 15-, 30-, and 60-min incubations, RNA synthesis was inhibited 20, 76 and 90% respectively, and lipid synthesis 35, 48, and 62% respectively; photosynthesis and protein synthesis were inhibited over 90% at all of these time periods. The effects of 50 M butachlor on protein and RNA synthesis in rice (Oryza sativa L.) and barnyardgrass (Echinochloa crusgalli L.) root and shoot segments were also investigated. Protein synthesis was inhibited in both species and to a greater degree in roots (81–90%) than in shoots (55–65%). RNA synthesis was inhibited 33% in barn-yardgrass roots but not significantly in barnyardgrass shoots or either organ of rice.     

Structural metabolic processes in the skeletal muscle tissue of chick embryos administered actinomycin D

The development of the skeletal muscle tissue has been studied cytophotometrically, electron microscopically and radioautographically at administration of actinomycin-D (0.2 mcg/g) to the 11- and 15-day-old chick embryos). Different character of restorative processes under the conditions when RNA synthesis is disturbed by actinomycin-D administration is noted: before morphologically distinguished myosatellites appear (before the 13th-14th day of embryogenesis) and after myosatellites appearance (from the 14th-15th day of development). Evidently, the myosatellites are the muscle cells resistive to certain external factors, they ensure an effective adaptation of the skeletal muscle tissue to unfavourable effects. When the satellite cells appear, the skeletal muscle tissue acquires a new quality as a dynamically stable cambial system.     

Butachlor influence on selected metabolic processes of plant cells and tissues

Time- and concentration-course studies were conducted to determine the effects of butachlor (N-[butoxymethyl]-2-chloro-2′,6′-diethylacetanilide) on photosynthesis, protein synthesis, RNA synthesis, and lipid synthesis using isolated leaf cells of red kidney bean (Phaseolus vulgaris L.). At the 2-h incubation period, butachlor inhibited photosynthesis, protein synthesis, RNA synthesis, and lipid synthesis 99, 99, 96, and 81% respectively at 100 μM, and 0, 19, 17, and 40% respectively at 10 μM. At 100 μM and 15-, 30-, and 60-min incubations, RNA synthesis was inhibited 20, 76 and 90% respectively, and lipid synthesis 35, 48, and 62% respectively; photosynthesis and protein synthesis were inhibited over 90% at all of these time periods. The effects of 50 μM butachlor on protein and RNA synthesis in rice (Oryza sativa L.) and barnyardgrass (Echinochloa crusgalli L.) root and shoot segments were also investigated. Protein synthesis was inhibited in both species and to a greater degree in roots (81–90%) than in shoots (55–65%). RNA synthesis was inhibited 33% in barn-yardgrass roots but not significantly in barnyardgrass shoots or either organ of rice.