共查询到20条相似文献,搜索用时 15 毫秒
1.
V V Zhulin 《Biulleten' eksperimental'no? biologii i meditsiny》1988,106(9):317-319
Male rats, whose mothers were given ethanol during pregnancy, were injected inorganic P32 into lateral brain ventricles. Some animals during 1 hour before decapitation were subjected to stress. Phosphatidylethanolamine, phosphatidylcholine and phosphatidylserine were isolated from neocortex and hippocamp. Prenatal alcohol treatment led to 30% inorganic P32 incorporation increase into neocortex phosphatidylcholine. Stress was followed by phosphatidylcholine synthesis level decrease in neocortex by 13% and in hippocamp by 26%. Amplitude of phospholipid synthesis alterations increased after both prenatal alcohol treatment and stress. The results show that prenatal alcohol treatment results in essential disfunction of brain phospholipids synthesis. 相似文献
2.
M Ia Maizelis A L Zabludovskii 《Biulleten' eksperimental'no? biologii i meditsiny》1976,82(9):1061-1064
The authors studied C14-leucine and S35-methionine incorporation into the brain tissue homogenates and protein from different parts of the brain of rats subjected to intrauterine hypoxia. Depression of protein synthesis in certain brain structures, particularly in the hyppocampus was observed alongside with the stimulation of the amino acid incorporation into proteins of the other parts of the brain. Changes of the amino acid penetration into tissue homogenates fialed to correlate with the rate of their incorporation into proteins in separate structures of the brain. Experimental results pointed to disfunction in the protein metabolism intensity and in the blood-brain barrier system occurring during the late ontogenesis in rats surviving the intrauterine hypoxia. 相似文献
3.
Natalia N. Nalivaeva Lilia Fisk Rosa M. Canet. Aviles Svetlana A. Plesneva Igor A. Zhuravin Anthony J. Turner 《International journal of peptide research and therapeutics》2003,10(5-6):455-462
Summary In the present study we have investigated the effect of prenatal hypoxia on expression of amyloid precursor protein (APP)
and some metallopeptidases, which regulate β-amyloid peptide (Aβ) levels (neprilysin (NEP) and endothelin-converting enzyme
(ECE-1)) in the cortex of rats during different periods of postnatal development. We have found that the level of APP in the
sensorimotor cortex (SMC) of rats, analysed by Western blotting, increases from days 1 to 5 of postnatal development and then
steadily decreases with age, with the most dramatic decline in the period from day 180 to 600. In the cortex of rats subjected
to prenatal hypoxia on day 13.5 of embryogenesis, the postnatal levels of APP were higher than in the control. Secretion of
the soluble form of APP (sAPP) by α-secretase was found to be the most active on day 30 of postnatal development and there
was a significant decrease in the production of sAPP after prenatal hypoxia. NEP was found to be expressed in the cortex of
rats only at the early stages of postnatal development and it was barely detectable in adult rats. The decline of NEP levels
during ageing might contribute to accumulation of Aβ in later life in humans. Prenatal hypoxia resulted in a significant decrease
of NEP expression on day 10, but its level was recovered when animals were preconditioned to mild hypoxia. A similar phenomenon
was observed when the expression of ECE-1 was analysed. Overall, prenatal hypoxia leads to significant changes in the levels
of APP and expression of metallopeptidases involved in amyloid metabolism during all postnatal life and preconditioning to
hypoxia appeared to be neuroprotective. 相似文献
4.
Abstract: The influence of prenatal hypoxia on subsequent brain development in the young rat was investigated by examining body and brain weight, cerebral cortex wet weight, protein and DNA concentrations, acetylcholinesterase (AChE) activity, 3-quinuclidinyl benzilate (QNB)-binding levels, the relative amounts of protein in various subcellular fractions, and the in vivo incorporation of [14 C]lysine into the protein of homogenate and subcellular fractions. Exposure of pregnant females to a mild hypoxia (9.1% Os, 10 h per day for the 9-11 days preceding birth) resulted in a reduced body weight in the pups at days 1 and 5 after birth; total cortical DNA was reduced but brain weight and protein content were unaffected, leading to a higher protein/DNA ratio in prenatally hypoxic pups. By 10 days of age these differences between prenatally hypoxic and control animals were no longer apparent. There were no differences between prenatally hypoxic and control animals in AChE and QNB binding per milligram cortex protein. The relative amount of synaptic membrane protein from the cerebral cortex was reduced at day 1 in prenatally hypoxic animals and the synaptic membrane fraction showed a higher level of incorporation of [14 C]lysine on days 1, 5, and 10. The developmental profile of [14 C]lysine incorporation showed a peak on day 10 which was higher in prenatally hypoxic rats. By 46 days after birth little difference could be found between prenatally hypoxic and control animals. 相似文献
5.
Ordian NE Pivina SG 《Rossi?skii fiziologicheski? zhurnal imeni I.M. Sechenova / Rossi?skaia akademiia nauk》2002,88(7):900-906
Prenatal stresses significantly reduce the anxiety level and enhance motor activity in mature male rats. An enhancement of the 5 alpha-reductase activity occurs in the hypothalamus, hippocampus, and the brain frontal cortx. The data obtained suggest that behavioural changes in mature male rats due to prenatal stresses may be a consequence of an increased forming of active progesterone metabolities in the brain. 相似文献
6.
D. S. Vasil’ev N. L. Tumanova I. A. Zhuravin 《Journal of Evolutionary Biochemistry and Physiology》2010,46(5):518-523
A comparative study of the nervous tissue and distribution of the spine apparatus protein synaptopodin was performed in all
layers of the brain sensorimotor cortex and hippocampal CAl area in control rats and in the rats exposed to hypoxia at E14
and E18. It was found that beginning from the 20th day of postnatal development, a statistically significant decrease of the
mean number of labile synaptopodin-positive spines in the stratum radiatum moleculare of the hippocampal area CAl was observed
in rats exposed to hypoxia both at E14 and E18. The decrease of the number of labile spines in the sensorimotor brain cortex
was revealed only in the I layer beginning from the 20th day after birth in the rats exposed to hypoxia at E14. Maximal differences
in the studied brain areas were observed in adult rats exposed to hypoxia at E14 in the neocortex—a decrease by 23 ± 10%,
in hippocampus—by 24 ± 8%, respectively. However, no increased degeneration of neurons was detected in adult animals. It is
suggested that disturbances in cognitive functions and in the capability for learning observed in rats after prenatal hypoxia
can be due to a decrease of the amount of the labile synaptopodin-positive spines, which leads to a change of the structural-functional
properties of neuronal networks and to a decrease of their plasticity. 相似文献
7.
Vasil'ev DS Tumanova NL Zhuravin IA 《Zhurnal evoliutsionno? biokhimii i fiziologii》2010,46(5):435-439
A comparative study of the nervous tissue and distribution of the spine apparatus protein synaptopodin was performed in all layers of the brain sensorymotor cortex and hippocampal CA1 area in control rats and in the rats submitted to hypoxia at E14 and E18. It was found that beginning from the 20th day of postnatal development, in rats submitted to hypoxia both at E14 and E18 there was observed a statistically significant decrease of the mean number of labile synaptopodin-positive spines in the stratum radiatum molecular of the hippocampus area CA1. The decrease of the number of labile spines in the sensorymotor brain cortex was revealed only in the I layer beginning from the 20th day after birth in the rats submitted to hypoxia at E14. Maximal differences in the studied brain areas were observed in adult rats (exposed to hypoxia at E14: in the neocortex--a decrease by 23 +/- 10%, in hippocampus--by 24 +/- 8%, respectively). In adult animals, the increased degeneration of neuzons was not detected. It is suggested that disturbances in cognitive functions and in the capability for learning observed in rats after prenatal hypoxia can be due to a decrease of the amount of the labile synaptopodin-positive spines, which leads to a change of the structural-functional properties of neuronal networks and to a decrease of their plasticity. 相似文献
8.
9.
Methylation‐reprogrammed Wnt/β‐catenin signalling mediated prenatal hypoxia‐induced brain injury in foetal and offspring rats
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Yingying Zhang Mengshu Zhang Lingjun Li Bin Wei Axin He Likui Lu Xiang Li Lubo Zhang Zhice Xu Miao Sun 《Journal of cellular and molecular medicine》2018,22(8):3866-3874
Prenatal hypoxia (PH) is a common pregnancy complication, harmful to brain development. This study investigated whether and how PH affected Wnt pathway in the brain. Pregnant rats were exposed to hypoxia (10.5% O2) or normoxia (21% O2; Control). Foetal brain weight and body weight were decreased in the PH group, the ratio of brain weight to body weight was increased significantly. Prenatal hypoxia increased mRNA expression of Wnt3a, Wnt7a, Wnt7b and Fzd4, but not Lrp6. Activated β‐catenin protein and Fosl1 expression were also significantly up‐regulated. Increased Hif1a expression was found in the PH group associated with the higher Wnt signalling. Among 5 members of the Sfrp family, Sfrp4 was down‐regulated. In the methylation‐regulating genes, higher mRNA expressions of Dnmt1 and Dnmt3b were found in the PH group. Sodium bisulphite and sequencing revealed hyper‐methylation in the promoter region of Sfrp4 gene in the foetal brain, accounting for its decreased expression and contributing to the activation of the Wnt‐Catenin signalling. The study of PC12 cells treated with 5‐aza further approved that decreased methylation could result in the higher Sfrp4 expression. In the offspring hippocampus, protein levels of Hif1a and mRNA expression of Sfrp4 were unchanged, whereas Wnt signal pathway was inhibited. The data demonstrated that PH activated the Wnt pathway in the foetal brain, related to the hyper‐methylation of Sfrp4 as well as Hif1a signalling. Activated Wnt signalling might play acute protective roles to the foetal brain in response to hypoxia, also would result in disadvantageous influence on the offspring in long‐term. 相似文献
10.
V N Vitvitski? M R Ventskovich 《Izvestiia Akademii nauk SSSR. Seriia biologicheskaia》1989,(2):285-290
The changes in biochemical processes in brain cortex in rats with experimental hypoxia and hypoxia with consequent transplantation of embryonic nervous tissue into brain of adults have been studied. A small increase (2-3 times) in incorporation of biosynthesis precursors was observed as a result of transplantation both in normal and in hypoxic rats. These changes could be observed 100 days after the transplantation. These changes in biosynthesis led to selective increase in the amounts of 40-45 and 35-39 kDa proteins, which are characteristic both for local trauma caused by transplantation and for general one caused by hypoxia. The effect observed may be explained as a reaction of nervous cells on damage, and not on the presence of an embryonic brain transplant. 相似文献
11.
E. I. Tyulkova L. A. Vataeva M. O. Samoilov 《Journal of Evolutionary Biochemistry and Physiology》2010,46(5):484-488
Activity of the phosphoinositide system of intracellular signalization was studied in offspring of rats exposed to severe
hypobaric hypoxia at the 14–16th (group 1) or the 18–20th day (group 2) of prenatal hypoxia. At the age of 15 days, in animals
of both experimental groups the basal level of triphosphoinositides in the brain cortex was shown to be elevated as compared
with control. In the group 1, this parameter also remains elevated in adult animals. Application of glutamate produces a more
pronounced increase of the inositephosphates in brain slices of the 15-day old rats of the group 1 than in slices of animals
of the control group. In the 15-day old rats of the group 2, as compared with control, the phosphoinositide response to glutamate
application was reduced. No changes in the inositephosphate levels were revealed after application of glutamate upon slices
of adult (the 90-day old) control animals and of adult rats of the group 2. In slices of adult rats of the group 1, on the
contrary, the glutamate application produced an increase of the inositephosphate content. The obtained data indicate essential
changes of the phosphoinositide metabolism in the brain of rats exposed to action of hypoxia at the period of prenatal development.
The character and the severity of these changes depend on the period of development when action of hypoxia occurs. 相似文献
12.
13.
Prenatal stress is known to cause neuronal loss and oxidative damage in the hippocampus of offspring rats. To further understand the mechanisms, the present study was undertaken to investigate the effects of prenatal stress on the kinetic properties of high-voltage-activated (HVA) Ca(2+) and K(+) channels in freshly isolated hippocampal CA3 pyramidal neurons of offspring rats. Pregnant rats in the prenatal stress group were exposed to restraint stress on days 14-20 of pregnancy three times daily for 45 min. The patch clamp technique was employed to record HVA Ca(2+) and K(+) channel currents. Prenatal stress significantly increased HVA Ca(2+) channel disturbance including the maximal average HVA calcium peak current amplitude (-576.52+/-7.03 pA in control group and -702.05+/-6.82 pA in prenatal stress group, p<0.01), the maximal average HVA Ca(2+) current density (-40.89+/-0.31 pA/pF in control group and -49.44+/-0.37 pA/pF in prenatal stress group, p<0.01), and the maximal average integral current of the HVA Ca(2+) channel (106.81+/-4.20 nA ms in control group and 133.49+/-4.59 nA ms in prenatal stress group, p<0.01). The current-voltage relationship and conductance--voltage relationship of HVA Ca(2+) channels and potassium channels in offspring CA3 neurons were not affected by prenatal stress. These data suggest that exposure of animals to stressful experience during pregnancy can exert effects on calcium ion channels of offspring hippocampal neurons and that the calcium channel disturbance may play a role in prenatal stress-induced neuronal loss and oxidative damage in offspring brain. 相似文献
14.
N. D. Nosenko 《Neurophysiology》1997,29(2):98-103
Effects of prenatal stress (daily 1-h-long immobilization of pregnant females at the 15th–21st days of pregnancy) on the formation
of sex-related dimorphism of the turnover of noradrenaline (NA) and dopamine (DA) in the preoptic area (POA) of the brain
and mediobasal hypothalamus (MBH) were studied in 10-day-old rats. Sex-related differences of the turnover of a functional
NA pool in the POA and DA pool in the MBH were demonstrated in intact control rats: a higher rate of the monoamine turnover
was observed in males. Prenatal stress abolished these sex-related differences and, at the same time, induced such differences
in the DA turnover in the POA. It is supposed that prenatal stress-evoked early modifications of sex-related dimorphism of
the catecholamine turnover in the brain can result in the development of remote disturbances in the neuroendocrine control
of reproduction and adaptation. 相似文献
15.
Li G Bae S Zhang L 《American journal of physiology. Heart and circulatory physiology》2004,286(5):H1712-H1719
Fetal programming has profound effects on cardiovascular function in later adult life. We tested the hypothesis that chronic hypoxic exposure during fetal development downregulates endogenous cardioprotective mechanisms in adult rats. Time-dated pregnant rats were divided between normoxic and hypoxic (10.5% O2 from days 15 to 21 of gestation) groups. The male progeny were studied at 2 mo of age. Rats were subjected to heat stress (42 degrees C for 15 min). After 24 h, hearts were excised and subjected to 30 min of global ischemia and 1 h of reperfusion. Prenatal hypoxia did not change adult rat body weight and heart weight, but significantly increased the cross-sectional area of a left ventricular (LV) myocyte. Heat stress significantly improved postischemic recovery of LV function in normoxic control rats, but not in prenatally hypoxic rats. The infarct size in the LV resulting from ischemia-reperfusion was reduced by the heat stress pretreatment in control rats, but not in prenatally hypoxic rats. In accordance, heat stress significantly increased LV myocardial content of heat shock protein 70 only in normoxic control rats. In addition, there was a significant decrease in the LV myocardial content of the PKC-epsilon isoform in prenatally hypoxic rats compared with control rats. We conclude that prenatal hypoxia causes in utero programming of hsp70 gene in the LV, leading to an inhibition of its response to heat stress and a loss of cardioprotection in later adult life. 相似文献
16.
Ordian NE Pivina SG Akulova VK Galeeva AIu 《Rossi?skii fiziologicheski? zhurnal imeni I.M. Sechenova / Rossi?skaia akademiia nauk》2006,92(9):1100-1110
The effects of prenatal stress on the hypothalamic-pituitary-adrenal (HPA) axis activity and brain glycocorticoid receptors were studied in neonatal male and female offspring, as well as the influence of neonatal glycocorticoid receptors blockade on hormonal stress reactivity of adult rats. The results showed that there were sexual differences in plasma corticosterone level and corticosteroid binding in the cortex and hypothalamus of 5-day old control rats. Prenatal stress increased basal level of corticosterone in female rats, decreased corticosterone binding in hypothalamus and hippocampus of male and female rats, and increased corticosteroid receptor level in the male cortex. Neonatal administration of glycocorticoid receptor antagonist did not change plasma corticosterone level in 5-day old rats, but prolonged hormonal stress response of the HPA axis in adult male rats and increased hormonal stress response in female ones. The character of the IIPA axis activity of male and female rats with neonatal blockade of glycocorticoid receptors correspond to hormonal stress response of prenatal stressed rats. These data suggest that change of brain glycocorticoid receptors function in neonatal period of development might be one of the mechanisms of prenatal stress influence on the HPA axis activity in the adulthood. 相似文献
17.
1. Inhibition of the rate of incorporation of [(35)S]methionine into protein by phenylalanine was more effective in 18-day-old than in 8-day-old or adult rat brain. 2. Among the subcellular fractions incorporation of [(35)S]methionine into myelin proteins was most inhibited in 18-day-old rat brain. 3. Transport of [(35)S]methionine and [(14)C]leucine into the brain acid-soluble pool was significantly decreased in 18-day-old rats by phenylalanine (2mg/g body wt.). The decrease of the two amino acids in the acid-soluble pool equalled the inhibition of their rate of incorporation into the protein. 4. Under identical conditions, entry of [(14)C]glycine into the brain acid-soluble pool and incorporation into protein and uptake of [(14)C]acetate into lipid was not affected by phenylalanine. 5. It is proposed that decreased myelin synthesis seen in hyperphenylalaninaemia or phenylketonuria may be due to alteration of the free amino acid pool in the brain during the vulnerable period of brain development. Amyelination may be one of many causes of mental retardation seen in phenylketonuria. 相似文献
18.
Rueda-Clausen CF Morton JS Dolinsky VW Dyck JR Davidge ST 《American journal of physiology. Regulatory, integrative and comparative physiology》2012,303(4):R418-R426
We have previously shown that adult offspring exposed to a prenatal hypoxic insult leading to intrauterine growth restriction (IUGR) are more susceptible to cardiovascular pathologies. Our objectives were to evaluate the interaction between hypoxia-induced IUGR and postnatal diet in the early development of cardiovascular pathologies. Furthermore, we sought to determine whether the postnatal administration of resveratrol could prevent the development of cardiovascular disorders associated with hypoxia-induced IUGR. On day 15 of pregnancy, Sprague-Dawley rats were randomly assigned to hypoxia (11.5% oxygen), to induce IUGR, or normal oxygen (control) groups. For study A, male offspring (3 wk of age) were randomly assigned a low-fat (LF, <10% fat) or a high-fat (HF, 45% fat) diet. For study B, offspring were randomized to either HF or HF+resveratrol diets. After 9 wk, cardiac and vascular functions were evaluated. Prenatal hypoxia and HF diet were associated with an increased myocardial susceptibility to ischemia. Blood pressure, in vivo cardiac function, and ex vivo vascular function were not different among experimental groups; however, hypoxia-induced IUGR offspring had lower resting heart rates. Our results suggest that prenatal insults can enhance the susceptibility to a second hit such as myocardial ischemia, and that this phenomenon is exacerbated, in the early stages of life by nutritional stressors such as a HF diet. Supplementing HF diets with resveratrol improved cardiac tolerance to ischemia in offspring born IUGR but not in controls. Thus we conclude that the additive effect of prenatal (hypoxia-induced IUGR) and postnatal (HF diet) factors can lead to the earlier development of cardiovascular pathology in rats, and postnatal resveratrol supplementation prevented the deleterious cardiovascular effects of HF diet in offspring exposed to prenatal hypoxia. 相似文献
19.
Reznikov AG Nosenko ND Tarasenko LV 《Comparative biochemistry and physiology. Toxicology & pharmacology : CBP》2004,138(2):169-175
We investigated the effects of hydrocortisone acetate and dexamethasone administered to pregnant rats during the last gestational week on sexual differentiation of testosterone metabolism and biogenic monoamine contents and turnover in the discrete brain regions in 10-day-old offspring. In the preoptic area, sex-dependent differences in aromatase activity were attenuated by prenatal glucocorticoids. Prenatal dexamethasone but not hydrocortisone acetate caused the inversion of sexual dimorphism of 5alpha-reductase activity in the preoptic area. In the brain preoptic area of the male pups prenatally exposed to hydrocortisone acetate, a decrease in noradrenaline turnover was found. Dopamine turnover in the preoptic area and 5-hydroxytryptamine metabolism in the preoptic area and medial basal hypothalamus increased in females as a result of hydrocortisone acetate treatment. Our results indicate that excess glucocorticoids in prenatal life modifies the basic neurochemical and neurophysiological mechanisms of sexual brain differentiation and might contribute to behavioral and reproductive disorders in adulthood. 相似文献
20.
I. Serra M. Alberghina M. Viola A. Mistretta A. M. Giuffrida 《Neurochemical research》1981,6(6):607-618
The effect of CDP-choline on the in vivo incorporation of labeled precursors into DNA, RNA, and proteins in cerebral hemispheres, cerebellum, and brainstem of guinea pigs after hypoxic treatment was studied. The labeling of macromolecules extracted from the various subcellular fractions of these brain regions was also determined. Hypoxic treatment affected macromolecular labeling to a different extent in the three brain regions examined. CDP-choline treatment was not able to reverse the effect of hypoxia on DNA labeling, but it was able to remove the effect of hypoxia on RNA and protein labeling. The action of CDP-choline was particularly evident on the labeling of RNA in nuclei and mitochondria of the cerebellum and on the labeling of proteins in microsomes of the three brain regions examined. 相似文献