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1.
R.D. Watson M.J. Ciancio W.P. Gunnar L.I. Gilbert W.E. Bollenbacher 《Journal of insect physiology》1985,31(6):487-494
The primary regulator of ecdysone biosynthesis by insect prothoracic glands is the prothoracicotropic hormone. However, it now appears that other factors, secondary regulators, may modulate prothoracic gland activity. One such factor has been isolated from the haemolymph of Manduca larvae. This haemolymph factor stimulates in vitro ecdysone synthesis by larval and pupal prothoracic glands by approx. 5-fold. It has an apparent mol. wt of ~330 kD, is protease-sensitive and is heat labile, the latter clearly distinguishing it from the prothoracicotropic hormone. Further, its steroidogenic effects and those of prothoracicotropic hormone are additive. Treatment of larval or pupal prothoracic glands with both moieties simultaneously effects an approx. 10-fold increase in ecdysone synthesis. The haemolymph titre of the stimulatory factor is low at commitment of the last-larval instar, then increases by approx. 3-fold later in the instar during pharate-pupal development. This increase in the titre is sufficient to effect a significant increase in prothoracic gland activity that could be physiologically important. Thus, it appears that the fluctuating level of this haemolymph stimulatory factor may act in conjunction with prothoracicotropic hormone to regulate the haemolymph ecdysteroid titre by modulating the ecdysone biosynthetic activity of the prothoracic glands. 相似文献
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The kinetics of secretion of ecdysone by the prothoracic glands of Locusta migratoria were studied during the last larval instar. Three stages of intense production of ecdysone (α-ecdysone) were monitored during this developmental period: they correspond to three peaks of moulting hormone concentration in the blood, which indicates that the main regulation of the moulting hormone titre is achieved through variations in prothoracic gland activity. In the haemolymph the ratio of ecdysone to ecdysterone (20-hydroxy-ecdysone) is in favour of ecdysone during the two first moulting hormone peaks ecdysterone being by far predominant over ecdysone at the time of the third (major) peak; these results support previous studies on the metabolic fate of injected labelled ecdysone during the same developmental period in Locusta migratoria. 相似文献
4.
The in vitro secretion of ecdysteroids from the prothoracic glands of larvae of Gryllus bimaculatus was analysed by HPLC-RIA. The primary product was identified as 3-dehydroecdysone (65-93%), with lesser amounts of ecdysone (7-35%). Production and release of ecdysteroids from the prothoracic glands are calcium-dependent. The rate of ecdysteroid release was low during the beginning and the end of the last two larval stages and high in between. Prothoracic glands from young adult females produced only minor amounts of ecdysteroids and ceased hormone production around day 4 after the moult. 相似文献
5.
Injection of the juvenile hormone analog (JHA) methoprene into day 3, fifthinstar larvae of Bombyx mori induced developmental arrest. Feeding activity declined, and the larvae remained as larvae for more than 2 weeks, after which they died. After JHA injection, the hemolymph ecdysteroid titer was low, and the prothoracic glands were almost inactive for 7 days. During this period, prothoracic glands were stimulated by prothoracicotropic hormone (PTTH) in vitro, indicating that JHA did not inhibit the competence of the glands to respond to PTTH. When brain-corpora cardiaca-corpora allata complexes were removed from intact fifth-instar larvae on day 4, the prothoracic glands became autonomously active and produced enough ecdysone for pupation. When PTTH injections were given to larvae previously injected with JHA (7 days before), the larvae recovered feeding activity, purged their guts, and pupated. Injections of 20-hydroxyecdysone into larvae that had been injected with JHA 7 days earlier induced larval molting. These results suggest that JHA affects both the brain and the prothoracic gland. 相似文献
6.
Thomas J. Kelly Edward P. Masler Belgaum S. Thyagaraja Robert A. Bell Richard B. Imberski 《Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology》1992,162(7):581-587
Summary Hemolymph ecdysteroid titers and in vitro prothoracic gland ecdysteroid synthesis have been examined in last-instar larval (5th instar) females of Lymantria dispar. Ecdysteroids were quantified by radioimmunoassay and characterized by co-elution with known standards of ecdysteroids on reverse-phase high-performance liquid chromatography. Analysis of hemolymph yielded ecdysone and 20-OH-ecdysone in ratios of 1:1 (day 6, shortly after attainment of maximum weight) and 1:28 (day 10, molting peak). Analysis of in vitro culture media from glands challenged with extracts of brains or retrocerebral complexes, or left unchallenged, revealed only immunoreactive material co-eluting with a known standard of ecdysone. Time-course studies of in vitro prothoracic gland ecdysone secretion demonstrated a major peak on day 10, 1–2 days prior to pupal ecdysis, and a small elevation on days 5–6. On days 5 and 6, 2.29±0.41 and 2.65±0.72 ng ecdysone per gland, respectively, were secreted in 6-h cultures. On day 10, 25.69±4.36 ng was secreted in 6-h culture. The ability of prothoracic glands of various ages to respond to brain extracts containing prothoracicotropic hormone activity was tested by determining an activation ratio for each day of the instar. The activation ratio was determined over a 90-min period by dividing the amount of ecdysone secreted by one member of a pair of prothoracic glands in the presence of brain extract by that of its contralateral control gland in Grace's medium. Prior to the addition of brain extract, the activity of the glands was allowed to subside to basal level for 180 min in Grace's medium. The activition ratio was highest on days 3–7 and fell throughout the remainder of the instar as the inherent ability of the prothoracic gland to maintain high levels of ecdysteroid synthesis in vitro in the absence of prothoracicotropic hormone increased. A two-phase in vitro assay for prothoracicotropic hormone was established using activition ratios. This assay showed saturable doseresponse kinetics for prothoracic gland ecdysone secretion and specificity to extracts prepared from brain or retrocerebral complexes. A comparable assay for prothoracicotropic hormone purification, based on net synthesis and requiring half the number of prothoracic glands was also established.Abbreviations
A
r
activation ratio
- HPLC
high performance liquid chromatography
- HPSEC
high performance size-exclusion chromatography
- PG
prothoracic gland
- PTTH
prothoracicotropic hormone
- RIA
radioimmunoassay 相似文献
7.
《Insect Biochemistry》1986,16(5):781-787
A recently described protein, found in the haemolymph of Manduca sexta larvae, stimulates ecdysone synthesis by both larval and pupal prothoracic glands in vitro. The mode of action of this haemolymph stimulatory factor has been investigated, particularly as it compares to the action of the cerebral neuropeptide, prothoracicotropic hormone (PTTH). Unlike PTTH, the haemolymph factor does not stimulate ecdysone synthesis via an increase in the level of cAMP in the prothoracic glands. The haemolymph factor requires extracellular calcium for maximal stimulation of the prothoracic glands, but in contrast to PTTH, significant activity is retained in calcium-free medium. Exposure of the prothoracic glands to the haemolymph factor results in enhanced steroidogenesis within 1 min. This rapid stimulation contrasts with the 10–20 min lag period observed following PTTH exposure. However, the prolonged activation elicited by brief exposure to PTTH is not observed following exposure of the glands to the haemolymph stimulatory factor. Rather, the factor appears to be required as a sustained stimulus in order to exert its steroidogenic effects. The data indicate that the mode of action of the haemolymph factor is distinctly different from that reported previously for PTTH, and are consistent with the hypothesized role of the factor as a carrier of a sterol precursor utilized in ecdysone synthesis. 相似文献
8.
昆虫成虫蜕皮激素研究进展 总被引:3,自引:0,他引:3
绝大多数成体昆虫羽化后,幼虫期间负责蜕皮激素合成的前胸腺即发生退化,但在一些内部生理及外部环境因子的调控下,某些成体组织(如生殖腺)可扮演类似前胸腺的角色合成与分泌蜕皮激素。蜕皮激素的功能发挥是经受体介导的,包括核受体(如EcR/USP)和膜受体(如DopEcR),它们广泛表达于成体许多组织,参与成虫行为、生殖、寿命、滞育及免疫应答等众多方面的调节,对维持基本的生理功能具有重要作用。就成虫蜕皮激素的产生组织及影响其滴度的因素、成虫蜕皮激素受体概述与组织分布、成虫蜕皮激素信号通路的功能发挥等研究进展方面加以综述。 相似文献
9.
《Insect Biochemistry》1986,16(1):143-147
The cellular mechanism of action of the cerebral neuropeptide, prothoracicotropic hormone (PTTH), was investigated in vitro using prothoracic glands from the tobacco hornworm, Manduca sexta. An involvement of cyclic AMP (cAMP) in PTTH-stimulated ecdysone synthesis was demonstrated as follows: (a) the steroidogenic effect of PTTH on prothoracic glands of day 3 fifth instar larvae and day 0 pupae was mimicked by agents (1-methyl-3-isobutylxanthine, dibutyryl cAMP and forskolin) which act by increasing intracellular levels of cAMP; and (b) PTTH stimulated the formation of cAMP in glands from both stages in a rapid, dose-dependent manner. However, a significant accumulation of cAMP in response to PTTH occurred only in larval prothoracic glands. In pupal glands, effects of the neuropeptide on cAMP synthesis were seen only in the presence of a phosphodiesterase inhibitor. Although cAMP is involved in PTTH action at both stages, it thus appears that the developmental state of the prothoracic glands influences the degree to which cAMP accumulates in response to the neurohormone. In addition to cAMP, it appears from the following that Ca2+ plays an essential role in mediating the steroidogenic effects of PTTH: (a) PTTH-stimulated ecdysone synthesis was blocked by omission of Ca2+ from the incubation medium; and (b) ecdysone synthesis was stimulated by the calcium ionophore A23187. Agents which act by increasing intracellular levels of cAMP enhanced ecdysone synthesis equally well in both the presence and absence of extracellular calcium. By contrast, cAMP formation stimulated by both PTTH and A23187 was completely dependent upon extracellular Ca2+. The results suggest a primary role for Ca2+ in mediating PTTH-stimulated synthesis of cAMP, with the cyclic nucleotide in turn stimulating ecdysone synthesis. 相似文献
10.
The time course of secretion of ecdysone in vitro by the prothoracic glands of Bombyx mori was studied through the penultimate and last-larval instars. Ecdysone was produced by the glands in high amounts by the penultimate instar at 72 and 84 h while the glands in the last instar exhibited a high activity over 4 days around the time of gut purge and thereafter. The glands in the penultimate instar produced ecdysone at a low level throughout the instar before the sharp peak of activity, when they became inactive and remained so for the first 3 days of the last instar after when they regained secretory activity. Sensitivity of the glands to prothoracicotropic hormone varied in accord with the changes in their secretory activity. Inactive glands were not stimulated by 22K-prothoracicotropic hormone. In addition, glands with maximal activity in the penultimate instar were insensitive to 22K-prothoracicotropic hormone. These results suggest that the prothoracic glands in the penultimate and last-instar larvae are physiologically different. 相似文献
11.
Pupal diapause in Heliothis zea is regulated by a temperature-sensitive mechanism which prevents ecdysone production despite the release of prothoracicotropic hormone. To determine how this mechanism functioned, donor prothoracic glands were implanted into prothoracic gland-ablated hosts to test their ability to produce ecdysone in a diapause-sustaining temperature of 19°C. Results of these experiments ruled out the possibility that ecdysis production was regulated by the nervous system or by a mechanism intrinsic to the prothoracic glands, and suggested that a humoral factor was required for diapause termination.Haemolymph injection experiments supported this humoral factor hypothesis, i.e. haemolymph from non-diapausing donor pupae terminated diapause in hosts maintained at 19°C, whereas haemolymph from diapausing donor pupae had no such effect. These findings indicate that the temperature-sensitive mechanism regulating H. zea diapause functions by controlling the availability of a humoral factor necessary for ecdysone production by the prothoracic glands. 相似文献
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In the absence of the prothoracic glands, fifth instar larvae of Locusta migratoria contain no demonstrable quantities of ecdysone and ecdysterone (assayed together in the Calliphora bioassay), whereas normal larvae show a high peak of ecdysone activity. The metabolic fate of injected radiolabelled ecdysone is found to be very similar in prothoracectomized larvae to that of normal larvae (hydroxylation rate, dehydrogenation of ecdysone and ecdysterone, inactivation rate). However, in the absence of the prothoracic glands, the larvae excrete radiolabelled ecdysone in their faecal material at a rate which is considerably higher than that of normal insects of the same age. These results are discussed in view of the regulation of the ecdysone titres by the prothoracic glands in L. migratoria. 相似文献
13.
Structural characterization and expression analysis of prothoracicotropic hormone in the corn earworm, Helicoverpa zea 总被引:5,自引:0,他引:5
The cDNA encoding prothoracicotropic hormone (PTTH), the brain neuropeptide that stimulates the prothoracic glands to synthesize ecdysone, was cloned from the corn earworm Helicoverpa zea (Hez). The amino acid sequence deduced from the cDNA indicates a molecular structure that is distinct from the PTTH's reported in other Lepidoptera, but all contain an identical proteolytic cleavage site and the seven cysteine residues that are essential for activity. Northern hybridization shows a single mRNA present in the brain-subesophageal ganglion complex. Using RT-PCR, we observed constant amounts of PTTH mRNA during larval development but large fluctuations at pupation and prior to adult eclosion. 相似文献
14.
The sensitivity of the prothoracic glands to juvenile hormone and prothoracicotropic hormone (PTTH) of penultimate (5th)-instar larvae of Mamestra brassicae was compared with that of the same-instar larvae destined for pupal ecdysis by allatectomy. The activity of the prothoracic glands was assessed using either moulting of isolated abdomens or ecdysone radioimmunoassay. Juvenile hormone application immediately after neck-ligation (which removes brain-corpora cardiaca-corpora allata complex) prevented prothoracic gland function in larvae at all stages. When larvae were allatectomized 12 hr after ecdysis, followed by neck-ligation at different times and given juvenile hormone immediately, the hormone inhibited the prothoracic glands of young larvae, but activated the prothoracic glands from day-5 or older larvae. Juvenile hormone I, juvenile hormone II and methoprene activated the prothoracic glands, but juvenile hormone III was relatively ineffective. Brain implantation instead of juvenile hormone application led to activation of the prothoracic glands at all stages.Allatectomy thus caused changes leading to metamorphosis including a transformation of the prothoracic glands from ‘larval’ to ‘pupal’ type. After this change these prothoracic glands were able to respond not only to PTTH but also to juvenile hormone just as in last-instar larvae. 相似文献
15.
Summary The content of moulting hormones has been determined in homogenates of isolated prothoracic glands and oenocytes during the 5th instar of the silkworm,Bombyx mori by means of the Calliphora bioassay.Prothoracic glands show variable activity in the production of moulting hormones, reaching a maximum near the end of the larval period. Comparable activities, but at higher levels, could be demonstrated in oenocytes. Controls with doubled quantities of tissue produced in a proportionate reaction in the bioassay. Fat bodies were inactive.Prothoracic glands and oenocytes incubated together resulted in a slower pupation index than would be expected from the sum of single determinations of oenocytes and prothoracic glands. This is explained by the ability of prothoracic glands to build conjugates of ecdysones.Die Arbeit wurde mit Unterstützung der Deutschen Forschungsgemeinschaft durchgeführt 相似文献
16.
Production of cyclic AMP and adenosine by the brain and prothoracic glands of Manduca sexta 总被引:1,自引:0,他引:1
Relatively large amounts of cyclic AMP are produced by the prothoracic glands (source of the insect moulting hormone or moulting hormone percursor) of the tobacco hornworm, Manduca sexta. Pharate pupal glands produce more cyclic AMP than early fifth instar larval glands, and the addition of aminophylline enhances cyclic AMP accumulation. The much lower cyclic AMP level in the absence of aminophylline indicates the presence of potent cyclic AMP phosphodiesterase activity. Brains (sources of the prothoracicotropic hormone) also produce cyclic AMP but at a lower rate. Brains efficiently produce adenosine from ATP while β-ecdysone inhibits adenosine formation in early fifth instar larval brains. β-Ecdysone stimulates adenyl cyclase in brains of both stages when aminophylline and fluoride are present but has no effect on cyclic AMP accumulation in prothoracic glands. The absence of fluoride greatly reduces the amount of cyclic AMP produced by prothoracic glands when aminophylline is present. No cyclic AMP is accumulated in prothoracic glands when both fluoride and aminophylline are absent or in brains when fluoride is absent, notwithstanding the presence of aminophylline. Other insect tissues were also analysed for cyclic AMP production and none showed levels nearly as high as the prothoracic glands, suggesting a close relationship between cyclic AMP production and the function of the gland. 相似文献
17.
《Insect Biochemistry》1987,17(2):291-301
We have synthesized a tritiated form of 2,14,22,25-tetradeoxyecdysone (5β-ketol) of high specific activity (115 Ci/mmol). We have examined the capacity of various tissues of Locusta migratoria to use this 5β-ketol, a putative precursor of ecdysone, in ecdysteroid biosynthesis. While larval prothoracic glands convert the radiotracer to labelled 14-deoxyecdysone they fail to hydroxylate the molecule to ecdysone itself. Other larval tissues, embryonic tissues or vitellogenic female ovaries are unable to convert the radiotracer to ecdysone, 20-hydroxyecdysone or 2-deoxyecdysone, the terminal products of biosynthesis in different developmental stages. Using subcellular preparations of prothoracic glands or follicle cells we have been unable to show a biological C-14 hydroxylation of 5β-ketol. It thus appears that the step of C-14 hydroxylation in the biosynthesis of ecdysteroids requires a substrate other than 5β-ketol. 相似文献
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In many metazoans, final adult size depends on the growth rate and the duration of the growth period, two parameters influenced by nutritional cues. We demonstrate that, in Drosophila, nutrition modifies the timing of development by acting on the prothoracic gland (PG), which secretes the molting hormone ecdysone. When activity of the Target of Rapamycin (TOR), a core component of the nutrient-responsive pathway, is reduced in the PG, the ecdysone peak that marks the end of larval development is abrogated. This extends the duration of growth and increases animal size. Conversely, the developmental delay caused by nutritional restriction is reversed by activating TOR solely in PG cells. Finally, nutrition acts on the PG during a restricted time window near the end of larval development that coincides with the commitment to pupariation. In conclusion, the PG uses TOR signaling to couple nutritional input with ecdysone production and developmental timing. 相似文献
20.
The endocrine mechanisms that regulate prothoracic gland (PG) activity in early stages of final larval instar of the silkworm Bombyx mori were investigated using a newly developed long-term cultivation system of the gland. The PGs dissected from day-0 fifth instar larvae did not secrete detectable amounts of ecdysone for the first 24 h in culture but started secretion within the next 2 days. The amount of secreted ecdysone increased day by day. When day-0 PGs were co-cultivated with corpora allata, however, they remained inactive for at least 8 days. PGs dissected from 1-day younger larvae (day-3 fourth instar larvae) secreted ecdysone for the first 24 h but stopped secretion for the next 24 h, followed by recovery of ecdysone secretory activity. By contrast, PGs from day-1 fourth instar larvae remained active throughout a cultivation period without any sign of inactivation. However, when the same glands were exposed to a high titer of 20-hydroxyecdysone for the second 24h in culture, they gradually lost their activity. These results indicate that PGs of fourth instar larvae are inactivated by ecdysteroid through a negative feedback mechanism and that thus inactivated PGs spontaneously recover ecdysone secretory activity in the early fifth instar unless inhibited by juvenile hormone. 相似文献