陕西原Hu一新种记述（蜻蜓目：原Hu科）

本文报道采自陕西省镇巴县的原Ｈｕ科一新种：汉中原ＨｕＰｒｏｄａｓｉｎｅｕｒａ ｈａｎｚｈｏｎｇｅｎｓｉｓ ｓｐ．ｎｏｖ．。     

辽宁原蚋属一新种（双翅目：蚋科）

本文报道采自辽宁省新宾县溪流中的一新种原蚋,辽宁原蚋Ｐｒｏｓｉｍｕｌｉｕｍｌｉａｏｎｉｎｇｅｎｓｅｓｐ．ｎｏｖ．,并对其成虫、蛹和幼虫的形态进行描述。该新种与西伯利亚的Ｐｒｏｓｉｍｕｌｉｕｍｈｉｒｔｉｐｅｓｄｉｍｉｎｕ－ｔｕｍＲｕｂｚｏｖ（１９５６）极相似。     

青海省原金蝗属一新种（直翅目：蝗总科）

本文记述青海省蝗虫一新种－巴颜喀拉原金蝗Ｅｏｋｉｎｇｄｏｎｅｌｌａ ｂａｙａｎｈａｒｅｎｓｉｓ ｓｐ．ｎｏｖ．,新种与西藏原金蝗Ｅｏｋｉｎｇｄｏｎｅｌｌａ ｔｉｂｅｔａｎａ（Ｍｉｓｔｓｈ．）及昌都原金蝗Ｅｏｋｉｎｇｄｏｎｅｌｌａ ｃｈａｎｇｔｕｎｉｃａ Ｙｉｎ近似。     

国产前原鹅观草的订正

对我国文献中记载的前原鹅观草（Ｒｏｅｇｎｅｒｉａｍａｙｅｂａｒａｎａ（Ｈｏｎｄａ）Ｏｈｗｉ）的标本和植物,与原产于日本的该种进行了比较形态学、细胞学研究,二者差异显著。作者认为我国所记载的该种种名应是山东鹅观草（Ｒｏｅｇｎｅｒｉａｓｈａｎｄｏｎｇｅｎｓｉｓ（Ｂ．Ｓａｌｏｍｏｎ）Ｊ．Ｌ．Ｙａｎｇ,Ｙ．Ｈ．ＺｈｏｕｅｔＹｅｎ）,其内稃先端钝圆,长为外稃的３／４,染色体数为２ｎ＝４ｘ＝２８,具ＳＹ染色体组,结实率达９０％以上,过去被错定为Ｒ．ｍａｙｅｂａｒａｎａ。而Ｒ．ｍａｙｅｂａｒａｎａ在日本系一天然杂种,其内稃先端尖,与外稃等长或稍短,染色体数为２ｎ＝６ｘ＝４２,具ＨＳＹ染色体组,结实率极低,仅０．２％～０．４％。     

原块菌属的分类界线及其地理分布

报道了原鬼笔科Ｐｒｏｔｏｐｈａｌｌａｃｅａｅ我国的一个新记录种──日本原块菌ｐｒｏｔｕｂｅｒａｎｉｐｐｏｎｉｃａＫｏｂａｙ．,原记载其分布于日本,近来在我国云南南部发现,属于东亚特有分布种。并讨论了原块菌属ＰｒｏｔｕｂｅｒａＭｏｌｌｅｒ的分类界线,同意前人把ＰｒｏｔｏｐｈａｌｌｕｓＭｕｒｒｉｌｌ、ＰｒｏｔｕｂｅｒｅｌｌａＩｍａｉｅｔＫａｗａｍｕｒａ和ＫｏｂａｖａｓｉａＩｍａｉｅｔＫａｗａｍｕｒａ作为原块菌属的异名;亦根据现代地理分布格局推断原块菌属原是一个热带、亚热带分布属。     

海水实验围隔中桡足类对海洋原甲藻摄食的研究

现场研究了发生严重海洋原甲藻（Ｐｒｏｒｏｃｅｎｔｒｕｍｍｉｃａｎｓ）水华后海水实验围隔中桡足类火腿许水蚤（Ｓｃｈｍａｃｋｅｒｉａｐｏｐｌｅｓｉａ）和长腹剑水蚤（Ｏｉｔｈｏｎａｓｐ．）的摄食强度．结果表明,体长在１２５ｍｍ以上的火腿许水蚤成体及其部分桡足幼体对海洋原甲藻的摄食率平均为１２１４０ｃｅｌｓ·ｉｎｄ－１·ｄ－１;体长不到１２５ｍｍ的部分火腿许水蚤桡足幼体和长腹剑水蚤成体及其桡足幼体的摄食率仅为１１３３ｃｅｌｓ·ｉｎｄ－１·ｄ－１．实验期间围隔中海洋原甲藻的浓度为８５０～１６１７０ｃｅｌｓ·ｍｌ－１,该藻的种群增长率为－０．００２～０．１５０．当海洋原甲藻细胞处于生长期时,桡足类的摄食不足以抑制该藻种群的增长,因此围隔中桡足类不可能阻止海洋原甲藻水华发生或使水华发生后迅速消退     

精原干细胞移植研究进展

精子发生 (ｓｐｅｒｍａｔｏｇｅｎｅｓｉｓ)是一个特殊的细胞分化过程。在这一过程中 ,精原干细胞 (ｓｐｅｒ ｍａｔｏｇｏｎｉａｌｓｔｅｍｃｅｌｌ)经过增殖分化形成具有特殊形态的精子。由于精子发生是在一个特定的环境中进行 ,缺乏体内外的研究模型 ,阻碍了这一领域的进展。 1994年开始的精原干细胞移植技术近几年取得了较大进展 ,已成为研究精子发生机理的重要模型。而且这一技术为治疗一些类型的雄性不育、保存某些需要化疗的癌症患者的生育能力以及转基因牲畜生产开辟了新的途径。本文全面综述精原干细胞移植的发展过程、最新进展和…     

受稻瘟病原真菌侵染的水稻早期表达基因的cDNA克隆

以亲和性与非亲和性两个稻瘟病原真菌小种（Ｍａｇｎａｐｏｒｔｈｅ ｇｒｉｓｅａ（Ｈｅｂｅｒｔ）Ｂａｒｒ）感染同一水稻品种（Ｏｒｙｚａｓａｔｉｖａ Ｌ．ｃｖ．Ｓｈｅｎｘｉａｎｇｇｅｎｇ Ｎｏ．４）的植株产生明显不同的致病和抗病反应,由此建立了有效的感染系统。应用差异显示技术获得两个在侵染早期具有诱导表达特征的ｃＤＮＡ克隆,其中一个同时在致病和抗病反应中进行早期诱导表达,但在抗病反应中的诱导相对早于其在     

S/D处理人纤维蛋白原的病毒灭活验证

在人纤维蛋白原制备工艺中增加Ｓ／Ｄ处理灭活病毒步骤,ＴＮＢＰ和Ｔｗｅｅｎ８０终浓度分别为０．３％和１％,在２５℃处理６小时能有效灭活指示病毒ＶＳＶ（〉３．７５Ｌｏｇ）、Ｓｉｎｄｂｉｓ（〉４．４６Ｌｏｇ）、ＨＩＶ（〉３．６７Ｌｏｇ）,盲传三代未检出病毒     

人胰岛素原在甲醇酵母（Pichia pastoris）中的高效表达

甲醇营养型酵母Ｐｉｃｈｉａ ｐａｓｔｏｒｉｓ在近十几年已被人们广泛用作外源基因表达的系统。表达的是可溶性蛋白,且胞外分泌。本研究系将外源基因（胰岛素原基因）连接到穿梭质粒ＰＨＩＬ－Ｓ１上,再通过同源重组到酵母染色体,筛选表达株。用１升发酵罐在甲醇诱导下可获得０．３ｇ／Ｌ胰岛素原的产率。     

Recent advances in the study of Kaposi's sarcoma-associated herpesvirus replication and pathogenesis

It has now been over twenty years since a novel herpesviral genome was identified in Kaposi's sarcoma biopsies. Since then, the cumulative research effort by molecular biologists, virologists, clinicians, and epidemiologists alike has led to the extensive characterization of this tumor virus, Kaposi's sarcoma-associated herpesvirus(KSHV; also known as human herpesvirus 8(HHV-8)), and its associated diseases. Here we review the current knowledge of KSHV biology and pathogenesis, with a particular emphasis on new and exciting advances in the field of epigenetics. We also discuss the development and practicality of various cell culture and animal model systems to study KSHV replication and pathogenesis.     

The structure, reproduction and taxonomy of Vidalia and Osmundaria (Rhodophyta, Rhodomelaceae)

Comprises species occurring mostly in subtidal habitats in tropical, subtropical and warm-temperate areas of the world. An analysis of the type species, V. spiralis (Sonder) Lamouroux ex J. Agardh, a species from Australia, establishes basic characters for distinguishing species in the genus. These characters are (1) branching patterns of thalli, (2) flat blades that may be spiralled on their axis, (3) width of the blade, (4) primary or secondary derivation of sterile and fertile branchlets and (5) position of sterile and fertile branchlets on the thalli. Application of the latter two characters provides an important basic method for separation of species into three major groups. Osmundaria , a genus known only in southern Australia, was studied in relation to Vidalia , and its separation from the Vidalia assemblage is not accepted. Species of Vidalia therefore are transferred to the older genus name, Osmundaria. Two new species, Osmundaria papenfussii and Osmundaria oliveae are described from Natal. Confusion in the usage of the epithet, Vidalia fimbriala Brown ex Turner has been clarified, and Vidalia gregaria Falkenberg, described as an epiphyte on Osmundaria pro/ifera Lamouroux, is revealed to be young branches of the host, Osmundaria prolifera.     

New or rare chromosome counts in Artemisia L. (Asteraceae, Anthemideae) and related genera from Kazakhstan

Fifteen chromosome counts of six Artemisia taxa and one species of each of the genera Brachanthemum, Hippolytia, Kaschgaria, Lepidolopsis and Turaniphytum are reported from Kazakhstan. Three of them are new reports, two are not consistent with previous counts and the remainder are confirmations of very scarce (one to four) earlier records. All the populations studied have the same basic chromosome number, x = 9, with ploidy levels ranging from 2x to 6x. Some correlations between ploidy level, morphological characters and distribution are noted.     

肝癌中HBV和HCV基因和抗原的分布及意义

采用原位分子杂交方法检测HCV RNA及HBV X基因;采用免疫组织化学方法研究HCV核心抗原,非结构区C33c抗原及HBxAg在肝细胞肝癌中的定位及分布.结果表明(1)HCV RNA、HBV X基因在肝细胞肝癌组织检出率分别为40%(55/136)和82%(112/136).HCV RNA定位于癌细胞的胞浆内,阳性细胞呈散在、灶状及弥漫分布三种形式;HBV X基因在肝癌细胞中的分布呈胞浆型、核型及核浆型,阳性细胞也呈上述三种分布形式;(2)HCV C33c抗原、核心抗原在肝细胞肝癌中的阳性率为81%(133/164)及86%(141/164).C33c抗原定位于癌细胞及肝细胞的胞浆内;核心抗原既定位于癌细胞核中,又可定位于胞浆中.C33c抗原阳性细胞以灶状分布为主;而核心抗原阳性细     

Selection with two alleles and complete dominance

For a plant selection model with frequency-independent viabilities, fertilities and selfing rates, it is shown that apart from global fixation, for certain parameter combinations a protected polymorphism and facultative fixation (either allele may become fixed according to initial frequencies) may both occur. Facultative fixation requires different selling rates for the dominant and recessive type. Protection of the polymorphism requires resource allocation for male and female function. In this connection the problem of purely genetically caused population extinction is discussed.
For general frequency dependence and regular segregation, the chances for establishment of a completely recessive gene are compared to those of a completely dominant gene. It is proven that the process of establishment of the recessive gene, despite a fitness advantage, may be considerably endangered by drift effects if random mating prevails. The recessive gene may reach the same effectivity in establishment as a dominant gene, only if the recessive homozygote mates exclusively with its own type during the period of establishment.