共查询到20条相似文献,搜索用时 15 毫秒
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mRNA degradation machines in eukaryotic cells 总被引:13,自引:0,他引:13
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Xiaokan Zhang Emral Devany Michael R. Murphy Galina Glazman Mirjana Persaud Frida E. Kleiman 《Nucleic acids research》2015,43(22):10925-10938
mRNA deadenylation is under the control of cis-acting regulatory elements, which include AU-rich elements (AREs) and microRNA (miRNA) targeting sites, within the 3′ untranslated region (3′ UTRs) of eukaryotic mRNAs. Deadenylases promote miRNA-induced mRNA decay through their interaction with miRNA-induced silencing complex (miRISC). However, the role of poly(A) specific ribonuclease (PARN) deadenylase in miRNA-dependent mRNA degradation has not been elucidated. Here, we present evidence that not only ARE- but also miRNA-mediated pathways are involved in PARN-mediated regulation of the steady state levels of TP53 mRNA, which encodes the tumor suppressor p53. Supporting this, Argonaute-2 (Ago-2), the core component of miRISC, can coexist in complexes with PARN resulting in the activation of its deadenylase activity. PARN regulates TP53 mRNA stability through not only an ARE but also an adjacent miR-504/miR-125b-targeting site in the 3′ UTR. More importantly, we found that miR-125b-loaded miRISC contributes to the specific recruitment of PARN to TP53 mRNA, and that can be reverted by the ARE-binding protein HuR. Together, our studies provide new insights into the role of PARN in miRNA-dependent control of mRNA decay and into the mechanisms behind the regulation of p53 expression. 相似文献
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The kinetics of mammalian gene expression. 总被引:3,自引:0,他引:3
J L Hargrove M G Hulsey E G Beale 《BioEssays : news and reviews in molecular, cellular and developmental biology》1991,13(12):667-674
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Vlad Elgart 《Biophysical journal》2010,98(12):2780-2784
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Processing and degradation of chloroplast mRNA 总被引:27,自引:0,他引:27