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Seed desiccation mechanisms co‐opted for vegetative desiccation in the resurrection grass Oropetium thomaeum
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Robert VanBuren Ching Man Wai Qingwei Zhang Xiaomin Song Patrick P. Edger Doug Bryant Todd P. Michael Todd C. Mockler Dorothea Bartels 《Plant, cell & environment》2017,40(10):2292-2306
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Andrea Ditzer Hans-Hubert Kirch Ambili Nair Dorothea Bartels 《Journal of plant physiology》2001,158(5):623
Expression of many genes is induced during dehydration in vegetative tissues of the desiccation tolerant resurrection plantCraterostigma plantagineum. The most abundant group of desiccation-related gene products belong to the LEA (= Late Embryogenesis Abundant) proteins. Here we describe structures and expression patterns of members of group 3 and group 4Lea genes fromC. plantagineum. The most intriguing observation is the strong conservation of repeat motifs inLea genes found across divers plant species includingC. plantagineum and non-desiccation tolerant plants. This conservation of structural elements leads to speculations about evolution of desiccation tolerance in the resurrection plant. 相似文献
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The role of lipid metabolism in the acquisition of desiccation tolerance in Craterostigma plantagineum: a comparative approach 总被引:1,自引:0,他引:1
Francisco Gasulla Katharina vom Dorp Isabel Dombrink Ulrich Zähringer Nicolas Gisch Peter Dörmann Dorothea Bartels 《The Plant journal : for cell and molecular biology》2013,75(5):726-741
Dehydration leads to different physiological and biochemical responses in plants. We analysed the lipid composition and the expression of genes involved in lipid biosynthesis in the desiccation‐tolerant plant Craterostigma plantagineum. A comparative approach was carried out with Lindernia brevidens (desiccation tolerant) and two desiccation‐sensitive species, Lindernia subracemosa and Arabidopsis thaliana. In C. plantagineum the total lipid content remained constant while the lipid composition underwent major changes during desiccation. The most prominent change was the removal of monogalactosyldiacylglycerol (MGDG) from the thylakoids. Analysis of molecular species composition revealed that around 50% of 36:x (number of carbons in the acyl chains: number of double bonds) MGDG was hydrolysed and diacylglycerol (DAG) used for phospholipid synthesis, while another MGDG fraction was converted into digalactosyldiacylglycerol via the DGD1/DGD2 pathway and subsequently into oligogalactolipids by SFR2. 36:x‐DAG was also employed for the synthesis of triacylglycerol. Phosphatidic acid (PA) increased in C. plantagineum, L. brevidens, and L. subracemosa, in agreement with a role of PA as an intermediate of lipid turnover and of phospholipase D in signalling during desiccation. 34:x‐DAG, presumably derived from de novo assembly, was converted into phosphatidylinositol (PI) in C. plantagineum and L. brevidens, but not in desiccation‐sensitive plants, suggesting that PI is involved in acquisition of desiccation tolerance. The accumulation of oligogalactolipids and PI in the chloroplast and extraplastidial membranes, respectively, increases the concentration of hydroxyl groups and enhances the ratio of bilayer‐ to non‐bilayer‐forming lipids, thus contributing to protein and membrane stabilization. 相似文献
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The resurrection plant Craterostigma plantagineum Hochst. is used as an experimental system to investigate desiccation tolerance in higher plants. A search for genes activated during early stages of dehydration identified the gene CpEdi-9, which is expressed in mature seeds and in response to dehydration in the phloem cells of vascular tissues of leaves. Elements for the tissue-specific expression pattern reside in the isolated promoter of the CpEdi-9 gene, as shown through the analysis of transgenic plants. The
CpEdi-9 promoter could be a suitable tool for expressing genes in the vascular system of dehydrated plants.
CpEdi-9 encodes a small (10 kDa) hydrophilic protein, which does not have significant sequence homologies to known genes. The predicted protein CpEDI-9 shares some physicochemical features with LEA proteins from plants and a nematode. Based on the unique expression pattern and on the nucleotide sequence we propose that CpEdi-9 defines a new class of hydrophilic proteins that are supposed to contribute to cellular protection during dehydration. This group of proteins may have evolved because desiccation tolerance requires the abundant expression of protective proteins during early stages of dehydration in all tissues.Abbreviations ABA
Abscisic acid
- ABRE
ABA-responsive element
- Edi
Early dehydration induced
- GUS
Glucuronidase
- LEA
Late embryogenesis abundant
- MU
Methylumbelliferone
This article is dedicated to Prof. Dr. Francesco Salamini on the occasion of his 65th birthday and his departure from the Max Planck Institute in Köln 相似文献
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Bartels D 《Integrative and comparative biology》2005,45(5):696-701
This review will focus on the acquisition of desiccation tolerancein the resurrection plant Craterostigma plantagineum. Molecularaspects of desiccation tolerance in this plant will be comparedwith the response of non-tolerant plants to dehydration. Uniquefeatures of C. plantagineum are described like the CDT-1 (Craterostigmadesiccation tolerance gene-1) gene and the carbohydrate metabolism.Abundant proteins which are associated with the desiccationtolerance phenomenon are the late embryogenesis abundant (=LEA)proteins. These proteins are very hydrophilic and occur in severalother species which have acquired desiccation tolerance. 相似文献
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Smith-Espinoza CJ Phillips JR Salamini F Bartels D 《Molecular genetics and genomics : MGG》2005,274(4):364-372
The resurrection plant (Craterostigma plantagineum) is desiccation tolerant. However, callus derived from this plant, when propagated in vitro, requires exogenously applied
abscisic acid (ABA) in order to survive desiccation. Treatment of callus tissue with ABA induces most of the genes that are
induced by dehydration in the whole plant. This property has been exploited for the isolation of mutants that show dominant
phenotypes resulting from the ectopic expression of endogenous genes induced by the insertion of a foreign promoter. Here
we describe new T-DNA tagged Craterostigma desiccation-tolerant (cdt) mutants with different molecular and physiological characteristics, suggesting that different pathways of desiccation tolerance
are affected. One of the mutants, cdt-2, constitutively expresses known osmoprotective Lea genes in callus and leaf tissue. Further analysis of this mutant revealed that the tagged locus is similar to a previously
characterised gene, CDT-1, which codes for a signalling molecule that confers desiccation tolerance. The nature of the T-DNA insertion provides insight
into the mechanism by which the CDT-1/2 gene family functions in ABA signal transduction. 相似文献
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In order to understand the molecular mechanisms which are responsible for desiccation tolerance in the resurrection plant
Craterostigma plantagineum Hochst. a thorough analysis of the CDeT11-24 gene family was performed. CDeT11-24 comprises a small gene family whose genes
are expressed in response to dehydration, salt stress and abscisic acid (ABA) treatment in leaves. The gene products are constitutively
expressed in roots and disappear only when the plants are transferred to water. It is therefore suggested that the proteins
are involved in sensing water status. The predicted proteins are very hydrophilic; they share some features with late-embryogenesis-abundant
proteins, and sequence similarities were found with two ABA- and drought-regulated Arabidopsis genes. The analysis of β-glucuronidase reporter genes driven by the CDeT11-24 promoter showed high activity in mature seeds
in both transgenic Arabidopsis and tobacco. In vegetative tissues the promoter activity in response to ABA was restricted to young Arabidosis seedlings. The responsiveness to ABA during later developmental stages was regained in the presence of the Arabidopsis gene product ABI3. Dehydration-induced promoter activity was only observed in Arabidopsis leaves at a particular developmental stage. This analysis indicates that some components in the signal transduction pathway
of the resurrection plant are not active in tobacco or Arabidopsis.
Received: 26 April 1997 / Accepted: 16 July 1997 相似文献
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Carbohydrate metabolism in a heat-girdled maize source leaf 总被引:6,自引:0,他引:6
E. Jeannette A. Reyss N. Grégory P. Gantet† and J.-L. Prioul 《Plant, cell & environment》2000,23(1):61-69