A review of the applications of solid state physics concepts to biological systems

The evidence for solid state physical processes in diverse biological systems is reviewed. Semiconduction of electrons across the enzyme particles as the rate-limiting process in cytochrome oxidase is evidenced by the peculiar kinetic patterns of this enzyme and by microwave Hall effect measurements. PN junction conduction of electrons is suggested by kinetics of photobiological free radicals in eye and photosynthesis. Superconduction at physiological temperatures may be involved in growth and nerve. Phonons and polarons seem likely to be involved in mitochondrial phosphorylation. Piezoelectricity and pyroelectricity may be involved in growth and nerve. Infrared electromagnetic waves may transmit energy in lipid bilayers of nerve and mitochondria. Complexed sodium and potassium ions in structured cell water may be analogous to valence band electrons in a semiconductor, and the free cations may be considered analogous to conduction band electrons. Ionic processes in cell water therefore resemble electronic conduction processes in solid semiconductors, which leads to kinetic predictions in agreement with experiment. The future of solid state biology depends on the development of new experimental methods able to measure solid state physical properties in biological materials which are non-crystalline, impure, particulate, and wet.     

Influence of the Hall effect on the plasma dynamics in an MTF/MAGO chamber

The role of the Hall effect in experiments on the magnetic implosion of a D-T plasma in a cylindrical MTF/MAGO chamber fed from a helical explosive magnetic generator is investigated. The plasma dynamics is simulated numerically by a 2D code developed for solving the set of MHD equations with account of the Hall effect. In simulations, the generator, the break switch, and other units were replaced with LR circuits. It is shown that taking into account the Hall effect provides better agreement between numerical simulations and experimental data.     

High efficiency Hall effect micro-biosensor platform for detection of magnetically labeled biomolecules

Detection of magnetically labeled biomolecules using micro-Hall biosensors is a promising method for monitoring biomolecular recognition processes. The measurement efficiency of standard systems is limited by the time taken for magnetic beads to reach the sensing area of the Hall devices. Here, micro-current lines were integrated with Hall effect structures to manipulate the position of magnetic beads via field gradients generated by localized currents flowing in the current lines. Beads were accumulated onto the sensor surface within seconds of passing currents through the current lines. Real-time detection of magnetic beads using current lines integrated with Hall biosensors was achieved. These results are promising in establishing Hall biosensor platforms as efficient and inexpensive means of monitoring biomolecular reactions for medical applications.     

Microbial Degradation of Forensic Samples of Biological Origin: Potential Threat to Human DNA Typing

Forensic biology is a sub-discipline of biological science with an amalgam of other branches of science used in the criminal justice system. Any nucleated cell/tissue harbouring DNA, either live or dead, can be used as forensic exhibits, a source of investigation through DNA typing. These biological materials of human origin are rich source of proteins, carbohydrates, lipids, trace elements as well as water and, thus, provide a virtuous milieu for the growth of microbes. The obstinate microbial growth augments the degradation process and is amplified with the passage of time and improper storage of the biological materials. Degradation of these biological materials carriages a huge challenge in the downstream processes of forensic DNA typing technique, such as short tandem repeats (STR) DNA typing. Microbial degradation yields improper or no PCR amplification, heterozygous peak imbalance, DNA contamination from non-human sources, degradation of DNA by microbial by-products, etc. Consequently, the most precise STR DNA typing technique is nullified and definite opinion can be hardly given with degraded forensic exhibits. Thus, suitable precautionary measures should be taken for proper storage and processing of the biological exhibits to minimize their decaying process by micro-organisms.     

Twisted fibrous arrangements in biological materials and cholesteric mesophases

A comparison is made between certain fibrous and regularly twisted biological materials and certain ordered liquids commonly called 'esteric liquid crystals' Three examples of twisted arrangements (Arthropod cuticle, Ascidian tunica, Dinoflagellate chromosomes) and typical textures of cholesteric mesophases are studied for their optical properties and their defects. These materials are strongly different. Very long polymer chains occur in the organic matrix of skeletal tissues or in chromosomes. On the contrary, in a cholesteric liquid crystal, the molecules are free to move one along the other. However the geometry of such systems is similar. The objections to the twisted model are reviewed and evidence is presented to support a generalized twisted model. A list of the known biological cholesteric analogues is given.     

Microwave Hall mobility measurements on rat liver mitochondria and spinach chloroplasts

The effect of known respiratory inhibitors on the charge carrier hall mobility of rat liver mitochondria has been investigated using a microwave technique. Potassium cyanide and rotenone were found to reduce the Hall mobility, but no effect was observed for antimycin-A. The marked effect of potassium cyanide and the low mobility value obtained for the lipid extract of the mitochondria, suggest that electronic conduction through the electron transport chain is being observed. Volume-corrected values of between 50 and 80 cm²/V sec are found for the electron Hall mobility.Measurements on spinach chloroplasts give P-type Hall mobility values of 0·5 and 0·8 cm²/V sec.     

Standard reference materials and data quality assurance in the biomedical analysis of trace elements

Accurate and precise analytical data of the concentrations of bioanalytes in bioclinical studies are of fundamental importance. Quality assurance procedures should always be performed to check the overall analytical work. This can be conveniently performed if appropriate standard reference materials with known concentrations of the analyte object of study are available. This paper underlines the key points related to the production and use of biological standard materials for trace element analysis. In particular, the present situation in the field of trace element determination in human biological fluids and the related problems are illustrated. The considerations given in this work may contribute to the preparation of the new biomarker standard materials.     

Biomolecular computing: Is it ready to take off?

Biomolecular computing is an emerging field at the interface of computer science, biological science and engineering. It uses DNA and other biological materials as the building blocks for construction of living computational machines to solve difficult combinatorial problems. In this article, notable advances in the biomolecular computing are reviewed and challenges associated with this multidisciplinary research are addressed. Finally, several perspectives are given based on the review of biomolecular computing.     

Recovery of volatile fatty acids from biological materials for direct analysis by gas chromatography

A simple and efficient methodology for preparing aqueous extracts of volatile fatty acids from biological materials, for direct analysis by gas chromatography is described. Peak areas and responses relative to n-butyric acid were used to calculate concentrations of the individual acids. An example is given for analysis of the volatile fatty acids found in the blood of the lugworm Aerinocola marina.     

Modeling of the tearing instability in unreduced two-fluid magnetohydrodynamics

The problem of the tearing instability is solved numerically in cylindrical geometry by using the unreduced two-fluid MHD model. It is shown that the duration of the nonlinear stage of the tearing instability in a hot plasma is rather sensitive to such factors as the initial radial density and temperature profiles, the initial ion-to-electron pressure ratio, and the longitudinal thermal conductivity. Depending on these factors, the two-fluid effects (primarily, the Hall effect) can either greatly hasten the magnetic reconnection process (in comparison to that in the one-fluid MHD model) or greatly slow it. An illustrative explanation of the results obtained is given.     

Study of the near-electrode processes in quasi-steady plasma accelerators with impenetrable electrodes

Near-electrode processes in a coaxial plasma accelerator with equipotential impenetrable electrodes are simulated using a two-dimensional (generally, time-dependent) two-fluid MHD model with allowance for the Hall effect and the plasma conductivity tensor. The simulations confirm the theoretically predicted mechanism of the so-called “crisis of current” caused by the Hall effect. The simulation results are compared with available experimental data. The influence of both the method of plasma supply to the channel and an additional longitudinal magnetic field on the development of near-electrode instabilities preceding the crisis of current is studied.     

Some factors affecting the future composition of UK compound animal feeds

Changes in the livestock compound-feed industry are outlined. It is noted that the change to least-cost formulation of diets manufactured to a strict nutritional specification has increased the economic and biological efficiency with which different types of feed have been utilised by livestock. Nevertheless, the feeding of livestock continues to depend on large quantities of raw materials which are potentially food for humans.A number of reasons are given why crop by-products and other waste materials are not fully utilised by the compound trade, although considerable efforts have been made by the industry to find economic and acceptable alternative raw materials for compound diets. In the case of BOCM Silcock, once a raw material has been successfully evaluated at the Unilever Research Laboratories at Colworth House, extensive development work is conducted at two development farms. Two different procedures are described and illustrated: (i) inclusion of the raw material in question at varying levels in a standard diet to assess effect on performance, and (ii) palatability test work, whereby a bland control-diet is partially replaced by the raw material under test, at levels appropriate to the likely commercial use of the test ingredient.However, the extent to which any waste material is eventually utilised will largely depend on its economic value, relative to the costs of competing raw materials. It appears unlikely that there will be any major availability of new cheap alternatives to cereals in the short term.     

A look at the possible mechanism and potential of magneto therapy.

A testable theoretical model for the mechanism of magneto-therapy is presented. The theory delineated is the equation mc2 = Bvl coulomb which sets in dual resonance gravitational and electromagnetic potentials. This proposed unification of Einstein's gravity and Maxwell's electromagnetism is designated Jacobson's resonance and is a general expression of Zeeman and cyclotron resonance. The application of this theory involves the utilization of exogenously sourced very weak magnetic fields on the order of magnitude 10(-8) gauss to reorient the atomic crystal lattice structures of genomic magnetic domains. Examples of genomic magnetic domains are homeoboxes and oncogenes and associated structures like peptide hormone trophic factors. Various phenomena are also analyzed in terms of how they may relate to biological systems such as solitons, phonons, cyclotron resonance, the piezoelectric effect, the fractional quantum Hall effect, string theory, and biologically closed electric circuits. The potential of magneto-therapy in the treatment of various genomic and associated disorders is explored. The ultimate question "Can an oncogene be electromagnetically induced into becoming a structurally homologous normal gene?" is posed.     

Low gravity on earth by magnetic levitation of biological material

The use of a magnetic field gradient levitation apparatus as a tool for investigating gravisensing mechanisms in biological systems and as a low gravity simulator for biological systems is described. The basic principles are described. Differences between its application to pure materials and the heterogeneous materials of biological materials are emphasized.     

Calibration of a Hall effect displacement measurement system for complex motion analysis using a neural network

Biomechanics studies often require the analysis of position and orientation. Although a variety of transducer and camera systems can be utilized, a common inexpensive alternative is the Hall effect sensor. Hall effect sensors have been used extensively for one-dimensional position analysis but their non-linear behavior and cross-talk effects make them difficult to calibrate for effective and accurate two- and three-dimensional position and orientation analysis. The aim of this study was to develop and calibrate a displacement measurement system for a hydraulic-actuation joystick used for repetitive motion analysis of heavy equipment operators. The system utilizes an array of four Hall effect sensors that are all active during any joystick movement. This built-in redundancy allows the calibration to utilize fully connected feed forward neural networks in conjunction with a Microscribe 3D digitizer. A fully connected feed forward neural network with one hidden layer containing five neurons was developed. Results indicate that the ability of the neural network to accurately predict the x, y and z coordinates of the joystick handle was good with r(2) values of 0.98 and higher. The calibration technique was found to be equally as accurate when used on data collected 5 days after the initial calibration, indicating the system is robust and stable enough to not require calibration every time the joystick is used. This calibration system allowed an infinite number of joystick orientations and positions to be found within the range of joystick motion.     

High-frequency extensions of magnetorotational instability in astrophysical plasmas

High-frequency extensions of magnetorotational instability driven by the Velikhov effect beyond the standard magnetohydrodynamic (MHD) regime are studied. The existence of the well-known Hall regime and a new electron inertia regime is demonstrated. The electron inertia regime is realized for a lesser plasma magnetization of rotating plasma than that in the Hall regime. It includes the subregime of nonmagnetized electrons. It is shown that, in contrast to the standard MHD regime and the Hall regime, magnetorotational instability in this subregime can be driven only at positive values of dlnΩ/dlnr, where Ω is the plasma rotation frequency and r is the radial coordinate. The permittivity of rotating plasma beyond the standard MHD regime, including both the Hall regime and the electron inertia regime, is calculated. Published in Russian in Fizika Plazmy, 2008, Vol. 34, No. 8, pp. 736–745.     

Reversal of the hall current and amplification of the magnetorotational instability in a weakly ionized plasma

A criterion for the development of a magnetorotational instability in a weakly ionized dusty plasma is considered. A dispersion relation for the wavenumber and the growth rate of an unstable perturbation is derived for an arbitrary angle between the wave vector and magnetic field. It is shown that the presence of dust grains can reverse the direction of the Hall current in the plasma and can shift the instability threshold to shorter wavelengths. Under certain conditions, Alfvén fluctuations of arbitrary scale can be unstable. The Hall current reversal is found to have a strong effect on the development of a magnetorotational instability when the Alfvén resonance frequency in a weakly ionized plasma is close to the rotation frequency of the accretion disk.     

Numerical technique of blood flow through catheterized arteries with overlapping stenosis

This study is concerned with the surgical technique for the injection of a catheter through arteries with overlapping stenosis in the presence of externally applied magnetic field and Hall currents influences. The nature of blood is analyzed mathematically by considering it as a micropolar fluid. The analysis is carried out for an artery with a mild stenosis. The governing equations with the corresponding boundary conditions solved numerically using Crank–Nicolson implicit finite difference scheme. The numerical technique give excellent agreement for axial velocity of the fluid, the circumferential microrotation, the wall shear stress distribution and the contour plots of stream lines. The obtained results show that the value of axial velocity is higher for a Newtonian fluid than that for a micropolar fluid model, the effect of suitable moving magnetic field (Hall currents influences) accelerates the speed of blood, the size of trapped bolus for the stream lines decrease if the spinning movement of the fluid molecules have considerable value regardless of small or large size of the fluid molecules and the flow of fluid is better with increasing the Hall current effect and the size of trapping bolus increase clearly by increasing the maximum height of stenosis where the fluid moves as a bulk.     

Biological activity of synthetic subunits of Streptococcus peptidoglycan. II. Relation of peptidoglycan subunits and analogues to fever effect and induction of tolerance

A series of synthetic subunits and analogues of streptococcal peptidoglycan was prepared and used in fever and tolerance experiments on rabbits. The lengthening of the chain of the peptide moiety of peptidoglycan did not result in pyrogenic activity, except for hexapeptide. Attachment of the muramyl residue rendered the peptides pyrogenic. The activity of such materials varied in degree and was rather in an indirect relation to peptide chain length. A change in the configuration of C4-OH or C3-OR in the muramyl residue resulted in a profound decrease in pyrogenicity. No inhibitory effect of N-acetylmuramyl-D-alanyl-D-isoglutamine on muramyldipeptide (MDP) pyrogenicity could be demonstrated. Repeated administration of MDP resulted in the induction of tolerance to the pyrogenicity of this substance in rabbits. These animals were not tolerant to the pyrogenicity of peptidoglycan. Nontolerance was also observed in reciprocal experiments with these materials as well as in trials with hexapeptide and peptidoglycan given in either order. The data are consistent with the assumption that peptidoglycan contains more than one biologically active subunit. There is a structure-to-function relationship. The knowledge of the biological effects of the synthetic analogues is essential for the prospect of their use under model or human conditions.