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Min Jee Kim Jong Seok Kim Jeong Sun Park Deuk‐Soo Choi Jinyoung Park Iksoo Kim 《Entomological Research》2015,45(6):354-359
The spotted‐wing drosophila (SWD), Drosophila suzukii (Diptera: Drosophilidae), originally distributed across a few Asian countries including South Korea, has invaded North America and Europe but is absent from Australia. In order to export the South Korean grape cultivar Campbell Early to Australia, its potential to serve as an oviposition and development medium for SWD must first be determined. In this study, we determined the oviposition and development potential of SWD on Campbell Early, after elucidating the SWD life cycle and establishing an artificial diet‐based mass‐culturing system. An investigation of the life cycle under five temperature regimes (16, 19, 22, 25 and 28°C) showed that the durations of the egg, larval and adult stages were shortened when temperature was increased from 16, 19, 22, 25 and 28°C, but pupal duration was shortest at 25°C and extended again at 28°C. A test of oviposition and development potential of SWD on Campbell Early grape clusters showed oviposition of 30.8 ± 6.8 eggs per cluster of injured grapes and 157.7 ± 16.2 eggs on a culture dish of artificial diet. However, in a similar experiment using uninjured grape clusters, only a single egg was deposited on the grape skin, which soon dried. In light of these results, newly harvested grapes left at vineyards during daily harvests are unlikely to serve as an oviposition and development medium for SWD, as long as the grapes remain uninjured. 相似文献
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Julia G. A. Vieira Alexandra P. Krüger Tiago Scheuneumann Amanda M. Garcez Maira C. Morais Flávio R. M. Garcia Dori E. Nava Daniel Bernardi 《Journal of Applied Entomology》2020,144(10):857-865
The aim of this study was to evaluate the effect of temperature (10, 15, 20, 25, 30 and 35°C), on the development time and life-time fecundity of Trichopria anastrephae Lima, 1940 (Hymenoptera: Diapriidae) parasitizing Drosophila suzukii (Matsumura, 1931) (Diptera: Drosophilidae). D. suzukii pupae that were up to 24-hr old were submitted to T. anastrephae parasitism for 24 hr. They were placed in plastic containers (50 ml) (ten pupae per container) in climatic chambers at temperatures of 10, 15, 20, 25, 30 and 35°C ± 1°C with a relative humidity of 70% ± 10% and a 12 hr photoperiod. For the adult phase, T. anastrephae couples that were up to 24 hr old were each placed in plastic cages (300 ml) and kept at the same temperatures cited above until their deaths. The higher numbers of parasitism and offspring production were obtained at temperatures between 15 and 25ºC. At the temperatures of 10 and 35ºC, there was no emergence of individuals. The lower thermal threshold (Tt) for the egg to adult period was ≈ 14.6°C for males and females with thermal constants (K) of 384.61 and 432.90, respectively. In terms of the fertility life table, T. anastrephae at 20 and 25°C presented shorter generation time (T) and higher net reproductive rates (Ro) in relation to other temperatures. The data show the ability of T. anastrephae to adapt to different thermal conditions, which is important for biological control programmes of D. suzukii. 相似文献
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室内实验云南德膏对樱桃果蝇Drosophila suzukii(Matsumura)成虫寿命的影响。结果显示,在取食含云南德膏(0·3%～5·0%)饲料后,樱桃果蝇成虫平均寿命显著延长,其中雌虫平均寿命延长率在98·61%～128·41%之间,雄虫在77·80%～114·14%之间;与对照相比,雌成虫半数存活天数延长率在104·17%～120·83%之间,雄虫延长率在95·65%～130·43%之间;雌虫平均最长存活天数延长率在90·10%～124·22%之间,雄虫在70·78%～114·14%之间。取食云南德膏纯品后,樱桃果蝇雌虫平均寿命比对照延长93·58%,雄虫延长84·50%;雌虫半数存活天数比对照延长112·50%,雄虫半数存活天数比对照延长108·70%左右。结果表明取食云南德膏可显著延长樱桃果蝇寿命。 相似文献
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Sergio Pérez-Guerrero;Laura Avivar-Lozano;José María Molina-Rodríguez; 《Journal of Applied Entomology》2024,148(5):493-501
Spotted-wing drosophila (SWD), Drosophila suzukii (Matsumura, 1931) (Diptera: Drosophilidae) cause yield losses and important economic impacts in fruits with thin epicarp. In the present study, the combination of powdered sulphur and predators/parasitoids for the control of SWD was analysed. First, the interaction of powered sulphur with Anthocoris nemoralis (Hemiptera: Anthocoridae) on infested strawberry fruits and Trichropia drosophilae (Hymenoptera: Diapriidae) on SWD pupae was evaluated. Second, exposure of predators/parasitoids to strawberry leaves with powdered sulphur was also assessed. Additionally, sublethal effects of powdered sulphur on T. drosophilae parasitism were examined. The results show powdered sulphur did not interfere with the activity of A. nemoralis and caused a significant reduction in fruit infestation. In contrast, sulphur affected the activity of T. drosophilae, reducing parasitism when the two agents were combined. Direct exposure to powdered sulphur did not cause significant mortality of A. nemoralis and T. drosophilae. Finally, previous exposure to sulphur did not affect T. drosophilae in terms of parasitism rates and sex ratio. Based on the results obtained herein, sulphur and T. drosophilae impacts on different stages of SWD when they are applied separately. However, the effects of sulphur on T. drosophilae should be taken into account in combination control strategies of SWD. 相似文献
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Tina Dancau Tanya L. M. Stemberger Peggy Clarke David R. Gillespie 《Biocontrol Science and Technology》2017,27(1):3-16
Drosophila suzukii and Drosophila melanogaster coexist with different but overlapping resource use in the field. When forced to completely or partially share resources in the laboratory, D. melanogaster outcompetes D. suzukii. Adult D. suzukii and D. melanogaster females were allowed to compete for access to a common oviposition resource in pairwise and population scale experiments. We tracked the offspring emergence to explore the factors across life stages that might affect the success of D. suzukii in the laboratory compared to a closed field simulation cage experiment with a generalist Drosophila parasitoid, Pachycrepoideous vindemmiae. When in competition as adults, D. melanogaster produced more offspring that survived to pupation than D. suzukii. In addition, D. melanogaster produced more offspring when in competition with a conspecific than when in competition with D. suzukii. Competitor identity did not affect the number of D. melanogaster offspring in pairwise and cage experiments. However, in the presence of D. melanogaster, the number of D. suzukii offspring in both pairwise and cage experiments was dramatically reduced than in cages without this competitor. In the presence of both D. melanogaster and P. vindemmiae, there were marginally more D. suzukii than when only D. melanogaster was present. These results suggest that competition was an important factor limiting D. suzukii numbers. Limiting D. suzukii numbers through interspecies competition may eventually be an exploitable method of biocontrol in the field. 相似文献
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Daniele C.H. Schlesener Jutiane Wollmann Alexandra P. Krüger Liliane N. Martins Cristiano M. Teixeira Daniel Bernardi Flavio R.M. Garcia 《Entomologia Experimentalis et Applicata》2020,168(11):817-826
Temperature is a determining factor for the development and establishment potential of insect pests. The present study describes the impact of temperature (13, 18, 23, 25, 28, 30, and 33 °C) on the life cycle parameters and phenotypic plasticity of South American populations of Drosophila suzukii (Matsumura) (Diptera: Drosophilidae) in the laboratory. Secondary objectives were to determine the lower thermal threshold and thermal constant to estimate the number of annual generations of the insect in small-fruit-producing regions in Brazil. The highest egg-to-adult survival was recorded at 23 and 25 °C. At 30 and 33 °C, no emergence of D. suzukii was observed. The egg-to-adult development time was shortest at 25 and 28 °C (ca. 10 days). The net reproductive rate (R0), and the intrinsic rate of population increase (rm) were highest at 23 and 25 °C. In contrast, temperatures of 13 and 28 °C generated largest and smallest body sizes, respectively, and caused reductions of 99 and 93% in R0. The estimated lower thermal threshold was 7.8 °C for egg-to-adult survival. The estimated thermal constant was 185.8 degree days, and the estimated annual number of generations of D. suzukii ranged from 17.1 in cold regions to 27.2 in warm regions. The results of the present study are important for understanding D. suzukii occurrence in the field, contributing to more informed and precise pest management. 相似文献
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Ibrahim Abdelhafiz;Stefan Gerth;Joelle Claussen;Mareike Weule;Eva Hufnagel;Andreas Vilcinskas;Kwang-Zin Lee; 《Advanced Biosystems》2024,8(7):2400100
Drosophila suzukii (D. suzukii), commonly known as the spotted wing drosophila, is a highly invasive crop pest that is difficult to control using chemical insecticides. To address the urgent need for alternative and more sustainable control strategies, the sterile insect technique (SIT) is improved, which involves the release of sterilized male insects to mate with fertile conspecifics, thereby reducing the size of the pest population in the subsequent generation. The three critical aspects that influence the success of SIT programs in D. suzukii are addressed. First, an accurate and nondestructive method is established to determine the sex of individual insects based on the differential weight of male and female pupae. Second, conditions for X-ray sterilization are systematically tested and an optimal dose (90 kV/40 Gy) is identified that ensures the efficient production of sterile D. suzukii for release. Finally, the inherent thermosensitivity of D. suzukii males is exploited to develop a temperature-based sterilization technique, offering an alternative or additional SIT method for this pest. These advances will contribute to the development of a comprehensive and effective strategy for the management of D. suzukii populations, reducing their impact on agriculture and helping to safeguard crop yields. 相似文献
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Sylvia M Durkin Mahul Chakraborty Antoine Abrieux Kyle M Lewald Alice Gadau Nicolas Svetec Junhui Peng Miriam Kopyto Christopher B Langer Joanna C Chiu J J Emerson Li Zhao 《Molecular biology and evolution》2021,38(6):2532
Studying how novel phenotypes originate and evolve is fundamental to the field of evolutionary biology as it allows us to understand how organismal diversity is generated and maintained. However, determining the basis of novel phenotypes is challenging as it involves orchestrated changes at multiple biological levels. Here, we aim to overcome this challenge by using a comparative species framework combining behavioral, gene expression, and genomic analyses to understand the evolutionary novel egg-laying substrate-choice behavior of the invasive pest species Drosophila suzukii. First, we used egg-laying behavioral assays to understand the evolution of ripe fruit oviposition preference in D. suzukii compared with closely related species D. subpulchrella and D. biarmipes as well as D. melanogaster. We show that D. subpulchrella and D. biarmipes lay eggs on both ripe and rotten fruits, suggesting that the transition to ripe fruit preference was gradual. Second, using two-choice oviposition assays, we studied how D. suzukii, D. subpulchrella, D. biarmipes, and D. melanogaster differentially process key sensory cues distinguishing ripe from rotten fruit during egg-laying. We found that D. suzukii’s preference for ripe fruit is in part mediated through a species-specific preference for stiff substrates. Last, we sequenced and annotated a high-quality genome for D. subpulchrella. Using comparative genomic approaches, we identified candidate genes involved in D. suzukii’s ability to seek out and target ripe fruits. Our results provide detail to the stepwise evolution of pest activity in D. suzukii, indicating important cues used by this species when finding a host, and the molecular mechanisms potentially underlying their adaptation to a new ecological niche. 相似文献
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Yue Chen Huan Wang Taibai Xu Peng Zhou Junsong Pan Weiwei She Weiwei Zhang 《Phyton》2023,92(4):1059-1070
Fruit spine is an important quality trait of cucumber. To better understand the molecular basis of cucumber spine development and function, RNA-Seq was performed to identify differentially expressed genes (DEGs) in fruit spines of different development stages, namely, 8 days before anthesis (SpBA8), anthesis (SpA) and 8 days after anthesis (SpAA8). Stage-wise comparisons obtained 2,259 (SpBA8 vs. SpA), 4,551 (SpA vs. SpAA8), and 5,290 (SpBA8 vs. SpAA8) DEGs. All the DEGs were classified into eight expression clusters by trend analysis. Among these DEGs, in addition to the Mict, Tril, CsTTG1, CsMYB6, NS, and Tu genes that have been reported to regulate fruit spine formation, we found that the CsHDG11, CsSCL8, CsSPL8, CsZFP6 and CsZFP8 may also be involved in spine development in cucumber. Our study provides a theoretical basis for further research on molecular mechanisms of spine development in cucumber. 相似文献
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S. Martos D. Spanò N. Agustí C. Poschenrieder F. Pintus L. Moles R. Medda 《The Annals of applied biology》2017,171(2):252-263
Drosophila suzukii attacks on developing soft fruits have recently caused important economic losses in Europe. This study explores the effectiveness of a new control strategy against this insect pest that is based on a plant chitinase extracted from the latex of the Mediterranean spurge, Euphorbia characias. The ability of the purified Euphorbia latex chitinase (ELC) to degrade the chitin exoskeleton of D. suzukii was assessed using confocal laser scanning microscopy. ELC treatment caused reduced larval growth, higher mortality and notable degradation of external insect structures. Therefore, the chitinase may induce a double effect on the D. suzukii larvae, a direct injury on the larval bodies and an action as antifeedant. The effects of the ELC treatment were also tested on leaves of the insect's host plants, Fragaria × ananassa and Rubus idaeus, using physiological parameters (chlorophyll concentration, chlorophyll fluorescence, leaf gas exchange and water potential) and defence gene expression (FaPGIP, FaChi2_1 and FaChi2_2) as stress indicators. ELC at concentrations effective against D. suzukii did not damage the host plants. Only plant defence gene expression was somewhat enhanced during the early hours after ELC application. In conclusion, ELC, a natural product, proved to be an effective tool for use in the development of an environmentally friendly integrated management strategy against D. suzukii, a pest whose control by conventional chemical insecticides is problematic. 相似文献
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制备果蝇心脏标记基因Hand抗体对研究果蝇心脏发育具有重要意义。从果蝇体内提取出总RNA,反转录得到果蝇的cDNA,将其作为模板PCR得到Hand基因部分片段,将片段连接到pET-28a上,构建重组质粒pET一28a—Hand,将重组质粒转化rosetta受体菌,IPTG诱导表达,表达产物经镍柱纯化,SDS—PAGE电泳分析,结果表明Hand基因在大肠杆菌中成功表达,表达的Hand融合蛋白分子量大约为24kD,经镍柱纯化后获得了高纯度可溶性的Hand蛋白。 相似文献
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K. S. Lim K. T. Lee J. E. Park W. H. Chung G. W. Jang B. H. Choi K. C. Hong T. H. Kim 《Animal genetics》2017,48(2):166-174
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【背景】作为发酵工业中一类重要的生产菌株,丝状真菌目的产物的形成与菌体形态有着紧密的联系。华根霉(Rhizopus chinensis)CCTCC M201021是从我国传统酿造浓香型白酒大曲中筛选到的一株丝状真菌,其生成的包括脂肪酶在内的酶蛋白具有较高的工业应用价值。【目的】华根霉(Rhizopus chinensis)CCTCC M201021在脂肪酶液态发酵中形成两种不同形态菌体,发酵表现差异明显。本研究考察华根霉不同菌体形态及其细胞代谢在转录组水平的内在差异。【方法】基于RNA-Seq高通量转录组测序,分别对液态培养获得的不同形态华根霉菌体高表达和显著差异表达的转录基因进行功能分析。【结果】两种形态菌体转录组存在明显的差异。利用RPKM值对表达量最高的前20个基因进行分析,聚集态菌体高表达基因主要为不同类别的核糖体蛋白,而分散态菌体中与细胞形态相关的几丁质酶及与信号传导相关的基因也是高表达基因。在两种形态菌体显著差异表达的20个基因中,除了涉及代谢的基因有明显不同外,分散态菌体中也有一些涉及\"细胞过程与信号\"的基因上调表达显著。两种形态菌体中独有表达基因总体表达量均较低,但聚集态菌体独有表达基因在基因种类和表达量上都要明显高于分散态菌体。同时,转录分析表明,华根霉脂肪酶在聚集菌体中较高的生产水平与脂肪酶基因的高水平转录有关。【结论】菌体形态的差异显著影响了华根霉的转录组,不仅不同形态菌体高表达基因和显著差异表达基因有明显不同,而且功能相同的蛋白在不同菌体形态下也多是由不同基因表达,它们可能承担着不同的作用。总体而言,华根霉聚集态菌体中存在更为复杂的生理过程,而分散菌体中受到信号的传导和调控似乎更多。菌体形态的改变可能是细胞分化的结果,伴随着菌体对细胞微环境改变的一种响应。研究结果为深入了解丝状真菌形态分化的内在机制及其影响提供了一些线索。 相似文献
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