共查询到20条相似文献,搜索用时 31 毫秒
1.
J. Triepel A. Weindl I. Kiemle J. Mader H. P. Volz M. Reinecke Prof. W. G. Forssmann 《Cell and tissue research》1985,241(1):31-41
Summary The distribution of substance P-immunoreactivity (SP-IR) in the brainstem and spinal cord of normal and colchicine-pretreated cats was analysed using the peroxidase-antiperoxidase (PAP) technique. Numerous SP-IR fibers are present in the nucleus solitarius, nucleus dorsalis nervi vagi and nucleus spinalis nervi trigemini, various parts of the formatio reticularis, substantia grisea centralis mesencephali, locus coeruleus and nucleus parabrachialis. SP-IR perikarya occur in the substantiae gelatinosa and intermedia of the spinal cord, the nucleus spinalis nervi trigemini-pars caudalis, the nucleus dorsalis nervi vagi, and the nucleus solitarius, as well as in the adjacent formatio reticularis and the medullary nuclei of the raphe. In addition, SP-IR cell bodies are located in the nuclei raphe magnus and incertus, ventral and dorsal to the nucleus tegmentalis dorsalis (Gudden), nucleus raphe dorsalis, substantia grisea centralis mensencephali, locus coeruleus, nucleus parabrachialis and colliculus superior.The results indicate that SP-IR neurons may be involved in the regulation of cardiovascular functions both at the central and peripheral level. A peripheral afferent portion seems to terminate in the nucleus solitarius and an efferent part is postulated to originate from the nucleus dorsalis nervi vagi and from the area of the nuclei retroambiguus, ambiguus and retrofacialis. 相似文献
2.
Triepel J. Mader J. Weindl A. Heinrich D. Forssmann W. G. Metz J. 《Histochemistry and cell biology》1984,81(6):509-516
Summary The occurrence and distribution of neurotensin-immunoreactive (NT-IR) perikarya was studied in the central nervous system of the guinea pig using a newly raised antibody (KN 1). Numerous NT-IR perikarya were found in the nuclei amygdaloidei, nuclei septi interventriculare, hypothalamus, nucleus parafascicularis thalami, substantia grisea centralis mesencephali, ventral medulla oblongata, nucleus solitarius and spinal cord. The distribution of NT-IR perikarya was similar to that previously described in the rat and monkey. In the gyrus cinguli, hippocampus and nucleus olfactorius, though, no NT-IR neurons were detected in this investigation. Additional immunoreactive perikarya, however, were observed in areas of the ventral medulla oblongata, namely in the nucleus paragigantocellularis, nucleus retrofacialis and nucleus raphe obscurus.The relevance of the NT-IR perikarya within the ventral medulla oblongata is discussed with respect to other neuropeptides, which are found in this area, and to cardiovascular regulation.Abbreviations abl
nucleus amygdaloideus basalis lateralis
- abm
nucleus amygdaloideus basalis medialis
- acc
nucleus amygdaloideus centralis
- aco
nucleus amygdaloideus corticalis
- ahp
area posterior hypothalami
- ala
nucleus amygdaloideus lateralis anterior
- alp
nucleus amygdaloideus lateralis posterior
- ame
nucleus amygdaloideus medialis
- atv
area tegmentalis ventralis
- bst
nucleus proprius striae terminalis
- CA
commissura anterior
- CC
corpus callosum
- cgld
corpus geniculatum laterale dorsale
- cglv
corpus geniculatum laterale ventrale
- cgm
corpus geniculatum mediale
- CHO
chiasma opticum
- CI
capsula interna
- co
nucleus commissuralis
- cod
nucleus cochlearis dorsalis
- cp
nucleus caudatus/Putamen
- cs
colliculus superior
- cu
nucleus cuneatus
- dmh
nucleus dorsomedialis hypothalami
- DP
decussatio pyramidum
- em
eminentia mediana
- ent
cortex entorhinalis
- epi
epiphysis
- FLM
fasciculus longitudinalis medialis
- fm
nucleus paraventricularis hypothalami pars filiformis
- FX
fornix
- gd
gyrus dentatus
- gp
globus pallidus
- gr
nucleus gracilis
- hl
nucleus habenulae lateralis
- hm
nucleus habenulae medialis
- hpe
hippocampus
- ift
nucleus infratrigeminalis
- io
oliva inferior
- ip
nucleus interpeduncularis
- LM
lemniscus medialis
- MT
tractus mamillo-thalamicus
- na
nucleus arcuatus
- nls
nucleus lateralis septi
- nms
nucleus medialis septi
- npca
nucleus proprius commissurae anterioris
- ns
nucleus solitarius
- n III
nucleus nervi oculomotorii
- nt V
nucleus tractus spinalis nervi trigemini
- ntm
nucleus mesencephalicus nervi trigemini
- osc
organum subcommissurale
- P
tractus cortico-spinalis
- PC
pedunculus cerebri
- PCI
pedunculus cerebellaris inferior
- pir
cortex piriformis
- pol
area praeoptica lateralis
- pom
area praeoptica medialis
- prt
area praetectalis
- pt
nucleus parataenialis
- pvh
nucleus paraventricularis hypothalami
- pvt
nucleus paraventricularis thalami
- r
nucleus ruber
- re
nucleus reuniens
- rgi
nucleus reticularis gigantocellularis
- rl
nucleus reticularis lateralis
- rm
nucleus raphe magnus
- ro
nucleus raphe obscurus
- rp
nucleus raphe pallidus
- rpc
nucleus reticularis parvocellularis
- rpgc
nucleus reticularis paragigantocellularis
- sch
nucleus suprachiasmaticus
- SM
stria medullaris thalami
- snc
substantia nigra compacta
- snl
substantia nigra lateralis
- snr
substantia nigra reticularis
- ST
stria terminalis
- tad
nucleus anterior dorsalis thalami
- tam
nucleus anterior medialis thalami
- tav
nucleus anterior ventralis thalami
- tbl
nucleus tuberolateralis
- tc
nucleus centralis thalami
- tl
nucleus lateralis thalami
- tmd
nucleus medialis dorsalis thalami
- TO
tractus opticus
- TOL
tractus olfactorium lateralis
- tpo
nucleus posterior thalami
- tr
nucleus reticularis thalami
- trs
nucleus triangularis septi
- TS
tractus solitarius
- TS V
tractus spinalis nervi trigemini
- tvl
nucleus ventrolateralis thalami
- vmh
nucleus ventromedialis hypothalami
- vh
ventral horn, Columna anterior
- zi
zona incerta
Supported by the Deutsche Forschungsgesellschaft (DFG) SFB 90, Carvas 相似文献
3.
Jochen Triepel Prof. Dr. med. Jürgen Metz Duncan Munroe Stephanie London Stephen Sweriduk Wolf Georg Forssmann 《Cell and tissue research》1987,249(1):145-150
Summary The distribution of vasoactive intestinal polypeptide-immunoreactive (VIP-IR) neurons in the lower medulla oblongata and the spinal cord has been analyzed in guinea pigs. This study includes results obtained by colchicine treatment and transection experiments. In the spinal cord, numerous VIP-IR varicosities were observed in the substantia gelatinosa of the columna dorsalis; some were also found in the substantia intermedia and the columna anterior. The spinal VIP-IR nerve fibers were mainly of intraspinal origin and oriented segmentally. VIP-IR nuclei in the spinal cord extended dorsally into corresponding regions of the caudal medulla oblongata, namely from the substantia intermedia medialis and lateralis into the vagus-solitarius complex and from the nucleus spinalis lateralis into the area of the nucleus reticularis lateralis. Additional VIP-IR perikarya were observed in the pars caudalis of the nucleus spinalis nervi trigemini. The VIP-IR nuclei within the caudal medulla oblongata probably form a continuous system with those localized within the spinal cord. They may be involved functionally in the modulation of cardiovascular and respiratory regulation in the guinea pig.Supported by the DFG, Carvas SFB 90 相似文献
4.
Antisera to N-acetylserotonin (NAS) were raised in rabbits by coupling NAS to bovine serum albumin (BSA) through a p-carboxybenzyl (PCB) bridge at the indole N. The specificity and applicability of these antisera in immunohistochemistry is reported. The anti-NAS antiserum and a fluorescein-labeled immunoglobulin were employed to investigate the topographic distribution of immunoreactive NAS (INAS) in the hindbrain (mesencephalon, cerebellum, pons, and medulla oblongata). Positive identification of INAS was confirmed in the granular layer of the cerebellum, the tractus spinalis nervi trigemini and the reticular formation. INAS was also identified in Purkinje cells, cerebellar nuclei, nucleus principalis nervi trigemini, nucleus tractus mesencephali, cochlear and vestibular nuclei, the locus coeruleus, and other brain stem regions. The pattern of INAS distribution is independent of serotonin (5-HT) and norepinephrine (NE), although certain loci could contain both INAS and serotonin or INAS and norepinephrine. 相似文献
5.
The distribution of binding site for [125I]-eel-calcitonin (ECT) to rat central nervous system, studied by an autoradiographic technique, showed concentrations of binding in the diencephalon, the brain stem and the spinal cord. Large accumulations of grains were seen in the hypothalamus, the amygdala, in the fasciculus medialis prosencephali, in the fasciculus longitudinalis medialis, in the ventrolateral part of the periventricular gray matter, in the lemniscus medialis and in the raphe nuclei. The density of grains in the reticular formation and in the nucleus tractus spinalis nervi trigemini was more moderate. In the spinal cord, grains were scattered throughout the dorsal horns. Binding of the ligand was displaced equally by cold ECT and by salmon CT(sCT), indicating that both peptides bind to the same receptors. Human CT was much weaker than sCT in displacing [125I]-ECT binding. The administration of ECT into the brain ventricles of rats dose-dependently induced a significant and long-lasting enhancement of hot-plate latencies comparable with that obtained with sCT. The antinociceptive activity induced by ECT is compatible with the topographical distribution of binding sites for the peptide and is a further indication that fish CTs are active in the mammalian brain. 相似文献
6.
Toshiya Matsushima Masahiko Satou Kazuo Ueda 《Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology》1989,166(1):7-22
1. To elucidate the neural mechanisms that mediate visual responses of optic tectum (OT) to medullary and spinal motor systems, we analyzed medullary reticular neurons in paralyzed Japanese toads (Bufo japonicus). We examined their responses to electrical stimulation of OT, and stained some neurons intracellularly. Responses to stimulation of the glossopharyngeal nerve (IX) were also analyzed. 2. Extracellular single unit recording revealed excitatory responses of medullary neurons to OT and IX stimulation. Among 92 units encountered, 79 responded to OT stimuli, 10 to IX stimuli, and 3 to both. Some units responded to successive stimuli of short intervals with relatively stable lags. 3. Intracellular recording and staining experiments revealed morphologies of reticular neurons that received excitatory inputs from OT. Thirteen units were identified after complete reconstruction of somata and dendrites. Neurons in the nucleus reticularis medius received excitatory inputs from bilateral OT. They had wide dendrites in ventral, ventrolateral and lateral funiculi, and single axons descending in the ipsilateral ventral funiculus as far caudally as the cervical spinal cord. Some collaterals of these axons projected directly to the hypoglossal and spinal motor nuclei. Some neurons in other medullary nuclei (nuc. reticularis superior, pretrigeminal nucleus, nuc. reticularis inferior, and nuc. tractus spinalis nervi trigemini) also responded to the OT stimulation. 4. Activities in bilateral OT converge onto medullary reticular neurons, which may directly control medullary and spinal motor systems. 相似文献
7.
Somatostatin-immunoreactive fiber projections into the brain stem and the spinal cord of the rat 总被引:2,自引:0,他引:2
Dr. Brigitte Krisch 《Cell and tissue research》1981,217(3):531-552
Summary By use of the PAP-immunohistochemical staining technique with serial sections, somatostatin-immunoreactive fiber projections into the brain stem and the spinal cord are described. These projections originate in the periventricular somatostatin-immunoreactive perikarya of the hypothalamus and form three main pathways: (1) along the stria medullaris thalami and the fasciculus retroflexus into the interpeduncular nucleus; (2) along the medial forebrain bundle into the mammillary body; and (3) via the periventricular gray and the bundle of Schütz into the midbrain tegmentum. Densely arranged immunoreactive fibers and/or basket-like fiber terminals are observed within the following afferent systems: somatic afferent systems (nucleus spinalis nervi trigemini, substantia gelatinosa dorsalis of the entire spinal cord), and visceral afferent systems (nucleus solitarius, regio intermediolateralis and substantia gelatinosa of the sacral spinal cord). These projections form terminals around the perikarya of the second afferent neuron. Perikarya of the third afferent neuron are influenced by somatostatin-immunoreactive projections into the auditory system (nucleus dorsalis lemnisci lateralis, nucleus corporis trapezoidei). Furthermore, a somatostatin-immunoreactive fiber projection is found in the ventral part of the medial accessory olivary nucleus, in nuclei of the limbic system (nucleus habenularis medialis, nuclei supramamillaris and mamillaris lateralis) and in the formatio reticularis (nucleus Darkschewitsch, nuclei tegmenti lateralis and centralis, nucleus parabrachialis lateralis, as well as individual perikarya of the reticular formation). Targets of these projections are interneurons within interlocking neuronal chains.Supported by the Deutsche Forschungsgemeinschaft (Grant Nr. Kr 569/3) and Stiftung Volkswagenwerk 相似文献
8.
Summary The afferent pathways to the nucleus basalis prosencephali of the pigeon were studied by use of the horseradish peroxidase (HRP) technique. It was confirmed that this nucleus receives a direct pathway from the nucleus sensorius principalis nervi trigemini and that, as in the starling, it receives a direct input from the nucleus lemnisci lateralis, pars ventralis, an auditory relay. Totally novel is the finding that the nucleus basalis prosencephali is the target of a direct pathway originating in the medullary nucleus vestibularis superior. All three pathways bypass the thalamus. From within the telencephalon the nucleus basalis prosencephali also receives fibres from the tuberculum olfactorium and the peri-ectostriatal belt, suggestive of olfactory and visual input. Marked cell bodies were also found in the neostriatum frontolaterale. It is assumed that these arose from HRP uptake by axons of the tractus fronto-archistriatalis that course through the nucleus basalis prosencephali to the anterodorsal archistriatum. Marked fibres and bouton-like formations were observed in the latter structure. The afferents to the nucleus basalis prosencephali are discussed in conjunction with the probable role of the nucleus as a sensorimotor coordinator of the pecking/feeding behaviour of the pigeon. 相似文献
9.
Summary Immunoreactive neurons were mapped in the central nervous system of colchicine-treated and untreated guinea pigs with the use of two antisera to the molluscan neuropeptide FMRFamide 1. These antisera were especially selected for their incapability to react with peptides of the pancreatic polypeptide family. Only one group of perikarya was stained by both antisera; this group was mainly located in the nucleus dorsomedialis hypothalami and extended to the nucleus paraventricularis and nucleus periventricularis hypothalami. The perikarya were found to project fibers to all regions of the hypothalamus, to the septum, nucleus proprius striae terminalis, nucleus paraventricularis thalami, nucleus centralis thalami, nucleus reuniens, medial, central and basal amygdala, area praetectalis, area tegmentalis ventralis of Tsai, substantia grisea centralis mesencephali, formatio reticularis mesencephali, nucleus centralis superior, locus coeruleus, nuclei parabrachiales, nucleus raphe magnus, A 5-region, vagus-solitarius complex, ventral medulla, nucleus spinalis nervi trigemini, and substantia gelatinosa of the spinal cord. In many brain regions FMRFamide-immunoreactive processes were found in close contact with blood vessels.Abbreviations of Amino Acids
D
aspartic acid
-
F
phenylalanine
-
G
glycine
-
H
histidine
-
L
leucine
-
M
methionine
-
P
proline
-
R
arginine
-
V
valine
-
W
tryptophan
-
Y
tyrosine 相似文献
10.
Ulrich Schall Juan D. Delius 《Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology》1986,159(1):33-41
Summary Evoked potentials were recorded from the nucleus basalis prosencephali (Bas) of the pigeon through chronically implanted electrodes. The auditory sensitivity of the Bas was assessed by the amplitude of the potentials. Audiograms thus obtained were comparable to those similarly measured from stations of the orthodox auditory pathway and resembled those obtained by others with behavioural techniques from the same species. The sensitivity to vibration applied to the beak was also measured. The vibrogram revealed two separate optima, one located in the lower frequency and another in the higher frequency region. These were shown to be due to trigeminal mechanoreceptive sensitivity and to bone/cochlea mediated sound sensitivity, respectively. Evoked potentials of the Bas in response to vestibular stimulation are described for the first time. The possibility that they were artefacts was excluded with several control procedures. These findings confirm recent anatomical evidence of a direct pathway from the vestibular nucleus to the nucleus basalis prosencephali. All afferents to the Bas are discussed in conjunction with the probable function of the nucleus as a sensorimotor coordinator of the pigeon's pecking/feeding behaviour.Abbreviations
A
archistriatum
-
aL
area L of the medial neostriatum caudale
-
Bas
nucleus basalis prosencephali
-
Cb
cerebellum
-
FA
tractus fronto-archistriatalis
-
HA
hyperstriatum accessorium
-
Hp
hippocampus
-
HRP
horseradish peroxidase
-
HV
hyperstriatum ventrale
-
LLv
nucleus lemnisci lateralis, pars ventralis
-
LPO
lobus parolfactorius
-
MV
nucleus motorius nervi trigemini
-
MLd
nucleus mesencephalicus lateralis, pars dorsalis
-
nVI
nucleus nervi facialis
-
nVIII
nervus vestibulocochlearis
-
N
neostriatum
-
NFL
neostriatum frontolaterale
-
OM
tractus occipitomesencephalicus
-
Ov
nucleus ovoidalis
-
PrV
nucleus sensorius principalis nervi trigemini
-
QF
tractus quintofrontalis
-
Rpv
nucleus reticularis parvocellularis, pars lateralis
-
TrO
tractus opticus
-
VS
nucleus vestibularis superior 相似文献
11.
Immunohistochemistry and radioimmunoassay (RIA) revealed that corticotropin releasing factor (CRF)-like immunoreactivity was found to be colocalized with substance P (SP)-, somatostatin (SST)- and leu-enkephalin (LENK)-like immunoreactivity in the dorsal root- and trigeminal ganglia, the dorsal horn of the spinal cord (laminae I and II), the substantia gelatinosa, and at the lateral border of the spinal nucleus and in the tractus spinalis of the trigeminal nerve. These peptides were also located in fast blue labeled cells of the trigeminal ganglion following injection of the dye into the spinal trigeminal area. This indicates that there are possible sensory projections of these peptides into the spinal trigeminal area. Capsaicin treatment of neonatal rats resulted in a marked decrease in the density of CRF-, SP-, VIP- and CCK-containing neurons in the above mentioned hindbrain areas, whereas SST- and LENK-immunoreactivity were not changed. RIA revealed that, compared to controls, CRF, SP and VIP concentrations in these areas were decreased in rats pretreated with capsaicin, while SST levels were increased; CCK and LENK levels were unchanged. It is concluded that the primary afferent neurons of the nucleus and tractus spinalis of the trigeminal nerve are richly endowed with a number of peptides some of which are sensitive to capsaicin action. The close anatomical proximity of these peptide containing neurons suggests the possibility of a coexistance of one or more of these substances. 相似文献
12.
By means of retrograde axonal transport of fluorescent tracers, connections between brainstem respiratory related regions and the spinal cord has been studied in the cat. Neurons at the pneumotaxic center project bilaterally (90% ipsi-, 10% contra-) to cervical and lumbar spinal cord and ipsilaterally to thoracic levels. The ventrolateral nucleus of the tractus solitarius project mainly contralaterally (85%) to cervical levels and only contralaterally to thoracic levels; no efferent projections were found to lumbar levels. The ventral respiratory group showed a great number of neurons projecting to the spinal cord especially from the nucleus retroambiguus. Both nuclei, ambiguus and retroambiguus, project mainly contralaterally (70%) to the spinal cord. The B?tzinger complex showed rather scarce bilateral projections to cervical and only ipsilateral projections to lower cervical, thoracic and lumber levels. 相似文献
13.
Thiamine monophosphatase: a genuine marker for transganglionic regulation of primary sensory neurons 总被引:3,自引:0,他引:3
E Knyihár-Csillik A Bezzegh S B?ti B Csillik 《The journal of histochemistry and cytochemistry》1986,34(3):363-371
Thiamine monophosphatase (TMPase) has been selectively localized in small dorsal root ganglion cells and in their central and peripheral terminals. Light microscopic localization of TMPase, and its alterations due to transganglionic effects, are identical with those of fluoride-resistant acid phosphatase (FRAP), but are not contaminated by the ubiquitous lysosomal reaction inevitable in trivial acid phosphatase-stained sections. TMPase is inhibited by 0.1 mM NaF, which is slightly less than the concentration needed to inhibit FRAP (0.2-0.4 mM). It is assumed that TMPase and FRAP are identical enzymes. In the perikaryon of small dorsal root ganglion cells, TMPase is located in the cisterns of the endoplasmic reticulum and in the Golgi apparatus. The central terminals of these cells are scalloped (sinusoid) axon terminals, surrounded by membrane-bound TMPase activity. Central terminals outline substantia gelatinosa Rolandi throughout the spinal cord, as well as the analogous nucleus spinalis trigemini in the medulla. TMPase-active central terminals outline "faisceau de la corne postérieure" in the sacral cord, as well as Lissauer's tract in the thoracic, upper lumbar, and sacral segments, and the paratrigeminal nucleus and the terminal (sensory) nucleus of the ala cinerea in the brainstem. Peripheral terminals displaying TMPase activity are fine nerve plexuses of C fibers. The TMPase activity of the central terminals disappears after dorsal rhizotomy in the course of Wallerian degeneration, and is depleted in the course of transganglionic degenerative atrophy (after transection of the related peripheral sensory nerve). TMPase is an outstanding genuine marker for the study of transganglionic regulation in Muridae. 相似文献
14.
实验家兔31只,在手术麻醉恢复后分别有对照地观察电流,谷氨酸钠,利多卡因作用于三叉神经起始核、终止核和脊束核的不同平面时对呼吸的影响。因兔的三叉神经脊束核呈纵向长条形,故对脊束核分别选其头端(A,2.3mm)中点(AP,0mm)和尾端(P,1.9mm)三个点刺激和观察。引导并记录膈神经放电及其积分曲线,呼出气CO_2分压曲线和血压曲线。用RM-6000型多道生理记录仪记录。 结果表明,电刺激和微量注射谷氨酸钠于脊束核和终止核,有明显加强呼吸的作用,但以同样的刺激作用于起始核,呼吸增强作用相对要小得多。微量注射利多卡因于起始核和终止核使呼吸减弱,但注射于脊束核,使呼吸加强,其原因有待进一步研究。本工作的结论是对三叉神经脊束核和终止核的刺激可以引起明显的呼吸改变。 相似文献
15.
《Neurochemistry international》1988,12(3):315-322
Noradrenaline and noradrenaline turnover were determined in regions enriched in catecholamine nuclei of the brainstem (A1, A2, C1, C2 and C3), in the locus coeruleus (A6) and in the nucleus tractus spinalis n. trigemini (Sp5C) of Wistar Kyoto and spontaneously hypertensive rats at four ages from 6 to 40 weeks. Adrenaline levels were also determined but were only consistently detected in the C1 and C2 regions. There was an age-related decline in noradrenaline concentrations in both strains of rats in all brainstem catecholamine nuclei however noradrenaline turnover decreased only in the A2 and C3 regions and this may contribute to the progressive rise in blood pressure with age in spontaneously hypertensive and Wistar Kyoto rats. Adrenaline levels did not alter with age providing evidence of a functional disassociation between adrenaline and noradrenaline neuronal systems. There were several strain-related differences in noradrenaline and adrenaline concentrations in the regions studied, however noradrenaline turnover was reduced only in the A2 and C2 regions (nucleus tractus solitarius) of spontaneously hypertensive rats which is consistent with the sympathoinhibitory role of this nucleus in central blood pressure regulation. 相似文献
16.
17.
Ozone (O(3)), a major component of air pollution, has considerable impact on public health. Besides the well-described respiratory tract inflammation and dysfunctions, there is accumulating evidence indicating that O(3) exposure affects brain functions. However, the mechanisms through which O(3) exerts toxic effects on the brain remain poorly understood. This work aimed at precisely characterizing CNS neuronal activation after O(3) inhalation using Fos staining in adult rat. We showed that, together with lung inflammation, O(3) exposure caused a sustained time- and dose-dependent neuronal activation in the dorsolateral regions of the nucleus tractus solitarius overlapping terminal fields of lung afferents running in vagus nerves. Furthermore, we highlighted neuronal activation in interconnected central structures such as the caudal ventrolateral medulla, the parabrachial nucleus, the central nucleus of the amygdala, the bed nucleus of the stria terminalis and the paraventricular hypothalamic nucleus. In contrast, we did not detect any neuronal activation in the thoracic spinal cord where lung afferents running in spinal nerves terminate. Overall, our results demonstrate that O(3) challenge evokes a lung inflammation that induces the activation of nucleus tractus solitarius neurons through the vagus nerves and promotes neuronal activation in stress-responsive regions of the CNS. 相似文献
18.
Hua F Harrison T Qin C Reifsteck A Ricketts B Carnel C Williams CA 《American journal of physiology. Heart and circulatory physiology》2004,287(6):H2728-H2738
The purpose of this study was to identify central neuronal sites activated by stimulation of cardiac ischemia-sensitive afferent neurons and determine whether electrical stimulation of left vagal afferent fibers modified the pattern of neuronal activation. Fos-like immunoreactivity (Fos-LI) was used as an index of neuronal activation in selected levels of cervical and thoracic spinal cord and brain stem. Adult Sprague-Dawley rats were anesthetized with urethane and underwent intrapericardial infusion of an "inflammatory exudate solution" (IES) containing algogenic substances that are released during ischemia (10 mM adenosine, bradykinin, prostaglandin E2, and 5-hydroxytryptamine) or occlusion of the left anterior descending coronary artery (CoAO) to activate cardiac ischemia-sensitive (nociceptive) afferent fibers. IES and CoAO increased Fos-LI above resting levels in dorsal horns in laminae I-V at C2 and T4 and in the caudal nucleus tractus solitarius. Dorsal rhizotomy virtually eliminated Fos-LI in the spinal cord as well as the brain stem. Neuromodulation of the ischemic signal by electrical stimulation of the central end of the left thoracic vagus excited neurons at the cervical and brain stem level but inhibited neurons at the thoracic spinal cord during IES or CoAO. These results suggest that stimulation of the left thoracic vagus excites descending inhibitory pathways. Inhibition at the thoracic spinal level that suppresses the ischemic (nociceptive) input signal may occur by a short-loop descending pathway via signals from cervical propriospinal circuits and/or a longer-loop descending pathway via signals from the nucleus tractus solitarius. 相似文献
19.
There have been few studies of the central projection of the sensory fibres of the recurrent laryngeal nerve into the medulla oblongata. In the present study terminal degeneration was observed in both the nucleus of the tractus solitarius and the nucleus of the spinal tract of the trigeminal nerve after transection of the nerve and after injection of a lectin, Ricinus communis agglutinin (RCA). Degeneration was observed to be more extensive after RCA injection than after nerve transection. In both instances, however, degeneration was observed bilaterally. 相似文献
20.
R Bortolami A Veggetti E Callegari M L Lucchi G Palmieri 《Archives italiennes de biologie》1977,115(4):355-385
The present research shows that sensory ganglion cells are located within the oculomotor nerve of monkeys and man. Furthermore, afferent fibers have been found in the IIIrd nerve of all the animals examined (lamb, pig, cat, dog and monkey). These fibers have their perikarya prevalently in the semilunar ganglion. Their pathway could be studied after section of either the trigeminal ophthalmic branch or of the intracranial portion of the IIIrd nerve. Following these operations, degenerating fibers were found entering the brain stem through the oculomotor nerve. In the brain stem, they were traced through the pons and the medulla and were seen to end in the spinal cord, within the subnucleus gelatinosus of the nucleus caudalis trigemini. Their degenerating endings found in the neuropil of the SG Rolandi, represented peripheral axonal endings of the glomeruli, rather than central axonal endings, as was the case after trigeminal rhizotomy. On the basis of these different degenerating patterns, the conclusion can be reached that the perikarya of the afferent fibers located in the semilunar ganglion represent, in reality, a ganglion of the IIIrd nerve. 相似文献