共查询到20条相似文献,搜索用时 31 毫秒
1.
2.
Delivering tissue regeneration. 总被引:1,自引:0,他引:1
W M Saltzman 《Nature biotechnology》1999,17(6):534-535
3.
4.
5.
6.
7.
8.
9.
C. William Castor M. Charlene Bignall Paul A. Hossler Dedra J. Roberts 《In vitro cellular & developmental biology. Plant》1981,17(9):777-785
Summary The quantitative radiochemical methodology described in this report allows a major increase in information generation, increased
experimental flexibility, improved statistical control, and increased diversity of information per culture. Other advantages
relate to economies of technical time, supplies, cells, and test materials per individual culture.
Microcultures of human synovial cells incorporate [14C]glucosamine into hyaluronic acid that accumulated primarily in the media and to a lesser extent in the cell mass. CTAP-I
(from lymphoid cells), CTAP-III (from human platelets), PGE2, dibutyryl cAMP, and poly(I)·poly(C) markedly stimulated hyaluronate synthesis, whereas cortisol, cycloheximide, and tunicamycin
inhibited stimulated synthesis. Time studies with cycloheximide indicated that translation, essential for the activation of
synovial cells, was completed by 17 h postexposure to CTAP-I. Tunicamycin also seemed to inhibit CTAP-I induced activation
primarily by interpering with translation; however, tunicamycin also caused modest post-translational inhibition of hyaluronate
synthesis in activated adult human synovial cells.
Study supported by U.S. Public Health Service Grant AM-10728, the Michigan Chapter of the Arthritis Foundation, and Michigan
Memorial-Phoenix Project Grant No. 517. 相似文献
10.
Martinod E Zakine G Fornes P Zegdi R d'Audiffret A Aupecle B Goussef N Azorin J Chachques JC Fabiani JN Carpentier A 《Comptes rendus de l'Académie des sciences. Série III, Sciences de la vie》2000,323(5):455-460
Tracheal reconstruction after extensive resection remains an unsolved surgical problem. Numerous attempts have been made using tracheal grafts or prosthetic conduits with disappointing results. In this study, we propose a new alternative using an aortic autograft as tracheal substitute. In a first series of experiments, a half circumference of two rings was replaced with an autologous carotid artery patch. In a second series, a complete segment of trachea was replaced with an autologous aortic graft supported by an endoluminal tracheal stent. No dehiscence or stenosis was observed. Microscopic examinations at 3 and 6 months showed the replacement of the aortic tissue by tracheal tissue comprising neoformation of cartilage and mucociliary or non-keratinizing metaplastic polystratified squamous epithelium. Although these results need to be confirmed by a larger series of experiments, they showed that a vascular tissue placed in a different environment with a different function can be submitted to a metaplastic transformation which tends to restore a normal structure adapted to its new function. These remarkable findings offer new perspectives in tracheal reconstruction in human. 相似文献
11.
Summary The progress in the development of the technologies of plant tissue and cell culture over the past four decades has been remarkable.
This article covers my personal reflections on the various topics and is based on my involvement in the field during that
period. There are three fundamental technologies which constitute most of what is referred to as plant in vitro technologies or tissue culture. The origin and some of the key persons involved in the development of each of these procedures
will be discussed. The technology that is most common is growing plant tissue on gel-solidified nutrient media. That technology
is being used in the most vital procedures, namely the regeneration of plants from cultured cells. The culture of plant cells
in liquid suspension was developed very shortly after that, and has become a very effective technology for plant regeneration
by somatic embryogenesis. The method of meristem culture arose out of a need for developing plants that were virus-free. In
many species the technique is now being used to produce virus-free crop plants. Another important technology is the culture
of anthers and microspores for producing haploid and homozygous plants. Included with plant tissue culture is the development
of the plant protoplast and cell fusion technologies for the production of new plant hybrids. The final aspect of the development
concerns the integration of tissue culture with molecular genetics, which has developed into the rapidly expanding field of
biotechnology. 相似文献
12.
13.
No significant differences could be found in the microbial quality of bruised and unbruised tissue provided that the two types of tissue were treated identically. This suggests that there is no good reason for the condemnation of bruised tissue, which could well be used in manufactured products. 相似文献
14.
15.
Endocrinology of adipose tissue. 总被引:7,自引:0,他引:7
16.
17.
18.
19.
No significant differences could be found in the microbial quality of bruised and unbruised tissue provided that the two types of tissue were treated identically. This suggests that there is no good reason for the condemnation of bruised tissue, which could well be used in manufactured products. 相似文献
20.
B Jones 《BMJ (Clinical research ed.)》1984,288(6414):345-346