黑盾鞭蝎血细胞分类研究

应用显微镜与相差显微镜相结合技术,对柄腹亚钢Ｃａｕｌｏｇａｓｔｒａ、有鞭目Ｕｒｏｐｙｇｉ、鞭蝎料Ｔｈｅｌｙｐｈｏｎｉｄａｅ的黑盾鞭蝎Ｔｙｐｏｐｅｌｔｉｓ ｎｉｇｅｒ血细胞进行分类研究,并与盲蛛（广腰亚纲、盲蛛目、长奇盲蛛科）比较,２种实验动物均具有原血细胞、浆细胞、颗粒细胞、类绛色细胞４种相同类型血细胞,但也存在不同类型囊细胞。     

宁夏六盘伪黑盲蛛记述(盲蛛目,开气门亚目,硬体盲蛛科)(英文)

记述了分布于中国宁夏回族自治区的伪黑盲蛛属Pseudomelanopa 1新种:六盘伪黑盲蛛P.liupan sp.nov.,此种与本属中的唯一种类台湾伪黑盲蛛P.taiwana相比较,雄蛛的阳茎和雌蛛的纳精囊明显不同;新种步足上的刺明显小于台湾伪黑盲蛛P.taiwana;新种雌蛛眼丘光滑,而台湾伪黑盲蛛P.taiwana眼丘上具1对小钝突;新种雄蛛触肢膝节和胫节光滑,仅在其膝节远端具1尖刺,而台湾伪黑盲蛛P.taiwana雄蛛触肢膝节和胫节背面具小齿.新种模式标本保存于河北大学博物馆.     

中国园蛛2雄蛛（蜘蛛目：园蛛科）

首次记述２种中国园蛛－类高居金蛛ＡｒｇｉｏｐｅａｅｈｅｒｏｉｄｅｓＹｉｎｅｔ．ａｌ．１９８９和双隆园蛛ＡｒａｎｅｕｓｐｒｏｍｉｎｅｎｓＹｉｎｅｔａｌ．１９８９的雄蛛。     

大腹园蛛血细胞相差显微镜的初步研究

本文应用相差显微镜技术研究了大腹园蛛(Araneus ventricous)的血细胞。结果发现大腹园蛛血淋巴中存在五种类型的血细胞:原始血细胞、浆细胞、顆粒细胞、类绛色细胞和脂血胞。每种类型的血细胞的发生与大腹园蛛的生长发育密切相关。首次发现类绛色细胞具有脱核现象。     

中国绿蟹蛛属一新种(蜘蛛目:蟹蛛科)

本文记述采自浙江宁波天童森林公司的蟹蛛科绿蟹蛛属一新种,钳绿蟹蛛Ｏｘｙｔａｔｅ ｆｏｒｃｉｐａｔｕｓ ｓｐ．ｎｏｖ。     

我国类石蛛科蜘蛛两新种：蜘蛛目：类石蛛科

本文记述产于云南省、湖南省和福建省类石蛛两新种：黑色垣蛛，新种Ａｒｉａｄｎａ ｐｅｌｉｏｓ ｓｐ．ｎｏｖ．；敏捷垣蛛，新种Ａｒｉａｄｎａ ｅｌａｐｈｒｏｓ ｓｐ．ｎｏｖ. 。     

湖南省5种皿蛛的记述（蜘蛛目：皿蛛科）

记述采自我国湖南省南岳、道县和双桥的皿蛛科皿蛛亚科蜘蛛５种,其中有４个新种：南岳斑皿蛛ｌｅｐｔｈｙｐｈａｎｔｅｓ ｎａｎｙｒｅｎｓｉｓ、衡山斑皿蛛Ｌｅｐｔｈｙｐｈａｎｔｅｓ ｈｅｎｈｓｈａｎｅｎｓｉｓ、双刺长指蛛Ｋａｅｓｔｎｅｒｉａ ｂｉｃｕｌｔｒａｔａ和双板指蛛Ｂａｔｈｙｐｈａｎｔｅｓ ｄｉｐｅｔａｌｕｓ,１个既有种：天目中指蛛Ｃｅｎｔｒｏｍｅｒｕｓ ｔｉａｎｍｕｓｈａｎｕｓ Ｃｈｅｎ ｅｔ Ｓ     

中国蟹蛛科（蜘蛛目）新种记述

记述我国蟹蛛科花蟹蛛属６新种和蟹蛛属２新种,共计８新种,金林花蟹蛛Ｘｙｓｔｉｃｕｓｊｉｎｌｉｎ,玛纳斯花蟹蛛Ｘ．ｍａｎａｓ,林芝花蟹蛛Ｘ．ｎｙｉｎｇｃｈｉｅｎｓｉｓ,拟斑花蟹蛛Ｘ．ｐａｒａｐｕｎｃｔａｔｕｓ,似旋花蟹蛛Ｘ．ｔｏｒｓｉｖｏｉｄｅｓ,吴氏花蟹蛛Ｘ．ｗｕａｅ,广西蟹蛛Ｔｈｏｍｉｓｕｓｇｕａｎｇｘｉｃｕｓ和胡氏蟹蛛Ｔ．ｈｕｉ。     

中国平腹蛛科两新属（蛛形纲：蜘蛛目）

本文记述了平腹蛛科两个新属及隶属于其中的３新种：异狂蛛属,新属Ａｌｌｏｚｅｌｏｔｅｓｇｅｎ．ｎｏｖ．,庐山异狂蛛,新种Ａ．ｌｕｓｈａｎｓｐ．ｎｏｖ．,滇池异狂蛛,新种Ａ．ＤＩＡＮＣＨＩＳＯ．ＮＯＶ．,     

横纹金蛛生物学特性的观察

横纹金蛛生物学特性的观察王昌贵,于红国,王翠珍（山东省日照市林业局日照２７６８００）关键词横纹金蛛,幼蛛,成蛛ＯＢＳＥＲＶＡＴＩＯＮＳＯＮＴＨＥＢＩＯＬＯＧＹＣＨＡＲＡＣＴＥＲＩＳＴＩＣＳＯＦＡｒｇｉｏｐｅｂｒｕｅｎｎｉｃｈｉ￥ＷａｎｇＣｈａｎｇｇｕ...     

激光共聚焦显微镜与光学显微镜之比较

激光扫描共聚焦显微镜在活细胞的动态检测、光学切片和三维结构重建等方面较光学显微镜有质的飞跃。本文对激光扫描共聚焦显微镜和光学显微镜进行了比较和讨论,并简单介绍多光子激光扫描显微镜。     

Functional comparative histology. 5. Communication: History of histology

The present author gives a survey on the development of microscopy by centuries: 16th Invention of the simple microscope--at the end of the century the compound microscope was invented. 17th HOOKE's microscope was the leading of this century--binocular microscope--introduction of screw fine adjustment. 18th The first microscope in America came to Yale in 1734. CHARLES SPENCER started to manufacture microscopes at the continent in 1847. 19th Establishment of precondition for electron microscopy. ZEISS introduced apochromatic optics. The Royal Microscopical Society was established in 1858. The American Microscopical Society in 1878, The New York Microscopical Society in 1877. 20th 1906 invention of the ultraviolet microscope, 1911 of the fluorescence microscope, 1931 of the electron microscope, the first American in 1938, 1932 the centrifuge microscope, 1932 the phase contrast microscope. The first American phase contrast microscope in 1944, 1942 phosphorescence microscope, in the 1950th the interference and x-ray microscopes.     

Imaging the internal and external pore structure of membranes in fluid: TappingMode scanning ion conductance microscopy.

We have constructed a combined TappingMode atomic force microscope and scanning ion conductance microscope. The design is based on a bent glass pipette that acts as both the force sensor and conductance probe. Measuring the pipette deflection allows more stable feedback than possible with previous versions of the scanning ion conductance microscope. Using this microscope, we have imaged synthetic membranes in both contact and tapping modes under fluid. Although contact mode operation is possible, we found that our microscope provided higher contrast and less apparent sample damage in the topographic and ionic conductance images in the tapping mode.     

一种目视激光显微镜

本文介绍一种目视激光显微镜。该装置采用白炽灯和激光做光源。通过调压器衬底亮度可以调整到零。由于激光的高亮度和强相干性,与普通显微镜相比,该显微镜具有景深长,分辨率高,层次丰富的特点。使用该显微镜时能实现镜象的假色彩编码,且镜象具有立体感。文中报导了该显微镜的原理和使用效果。     

Improvement of the morphometry method and its use in electron microscopic studies

The paper describes morphometric device for electron microscope. Using this gauge one can get morphometric data from the screen of the electron microscope. Perspectives of morphometric analysis of ultra-structures in the electron microscope are discussed.     

Atomic force microscopy in biology: technology and techniques

In this review, I describe the biological applications of the atomic force microscope (AFM). The historical background and the development of the microscope are described. The AFM can operate in many different modes relevant to biological systems including topography, chemical analysis, and forces relevant at the biological length scale (single cell to DNA dimensions and pico to nano Newton forces). A limited number of examples from the literature are described to illustrate some of the many capabilities of this microscope. The aim is to give an introduction of the technique to the inexperienced in this rapidly growing field.     

Atomic force microscopy in biology: technology and techniques.

In this review, I describe the biological applications of the atomic force microscope (AFM). The historical background and the development of the microscope are described. The AFM can operate in many different modes relevant to biological systems including topography, chemical analysis, and forces relevant at the biological length scale (single cell to DNA dimensions and pico to nano Newton forces). A limited number of examples from the literature are described to illustrate some of the many capabilities of this microscope. The aim is to give an introduction of the technique to the inexperienced in this rapidly growing field.     

Atomic force microscopy in biology: technology and techniques

In this review, I describe the biological applications of the atomic force microscope (AFM). The historical background and the development of the microscope are described. The AFM can operate in many different modes relevant to biological systems including topography, chemical analysis, and forces relevant at the biological length scale (single cell to DNA dimensions and pico to nano Newton forces). A limited number of examples from the literature are described to illustrate some of the many capabilities of this microscope. The aim is to give an introduction of the technique to the inexperienced in this rapidly growing field.     

Frequency division multiplexed multichannel high-speed fluorescence confocal microscope

In this article, we report a new type of fluorescence confocal microscope: frequency division multiplexed multichannel fluorescence confocal microscope, in which we encode the spatial location information into the frequency domain. In this microscope, the exciting laser beam is first split into multiple beams and each beam is modulated at a different frequency. These multiple beams are focused at different locations of the target to form multiple focal points, which further generate multiple fluorescent emission spots. The fluorescent emissions from different focal points are also modulated at different frequencies, because the exciting beams are modulated at different frequencies (or difference carrier frequency). Then, all the fluorescent emissions (modulated at different frequencies) are collected together and detected by a highly sensitive, large-dynamic-range photomultiplier tube. By demodulating the detected signal (i.e., via the Fourier transform), we can distinguish the fluorescent light emitted from the different locations by the corresponding carrier frequencies. The major advantage of this unique fluorescence confocal microscope is that it not only has a high sensitivity because of the use of photomultiplier tube but also can get multiple-point data simultaneously, which is crucial to study the dynamic behavior of many biological process. As an initial step, to verify the feasibility of the proposed multichannel confocal microscope, we have developed a two-channel confocal fluorescence microscope and applied it to study the dynamic behavior of the changes of the calcium ion concentration during the single cardiac myocyte contraction. Our preliminary experimental results demonstrated that we could indeed realize multichannel confocal fluorescence microscopy by utilizing the frequency division multiplexed microscope, which could become an effective tool to study the dynamic behavior of many biological processes.     

Thirty years of the electron microscope investigation in zoology and parasitology in the Zoological Institute of the Russian Academy of Sciences

The history of the electron microscope investigations in zoology and parasitology in the Zoological Institute of the Russian Academy of Sciences and progress in scanning and transmission electron microscope investigations in this field of biology to the moment are briefly accounted.