恙螨研究ⅩⅤⅢ. 鸡新勋恙螨Neosch?ngastia gallinarum Hatori, 1920若虫的畸形和变异形态(真螨目:恙螨科)

目前由于恙螨生活史的复杂性,若虫和成虫营自生生活而不易采得,幼虫则营寄生生活能在宿主体外检获,因此到目前止还是以幼虫的鉴别特征作为恙螨的分类依据,这在整个动物的分类学中也属罕见的。 研究恙螨的形态及分类学者如 Richard,1950;Sasa and Jameson,1954; Vercam-     

高湖纤恙螨生活史的研究

恙螨生活史的研究,对于进一步了解恙虫病立克次体在恙螨整个生活周期中的传播方式、研究恙虫病的感染过程和流行规律具有重要意义。本文比较详细地描述在我国浙江发现的高湖纤恙螨(Lepto-trombidium(Leptotrombidium)gaohuensis Wei et al.,1957)生活史及习性。 高湖纤恙螨的生活史分为七期:卵、次卵、幼虫、若蛹、若虫、成蛹及成虫,分述于下: 一、卵及次卵 初生卵为淡黄色,球形,大小为112.5×112.5毫微米,卵壳光裸。在28℃、相对湿度80％的条件下饲养,3—4天卵壳开始膨大,表面由光裸变为粗糙,5—8天延卵壳中轴分为两半球,一     

地里恙螨生活史和交配研究

本文报导在实验室中用集体饲养和单个饲养法所观察的地里恙螨第四代至第五代的生活史。对于卵、次卵、幼虫、若蛹、若虫和成蛹等各期的发育过程与发育时间作了记述;观察了雌雄成虫间接交配的过程, 记录了雄螨产置精胞和受精雌螨产生卵的数量、雌雄螨的不同寿命、以及摄食蚤卵的数量。 文内讨论了各发育期的淘汰率及其因素、卵与次卵、若蛹、成蛹等静止阶段的发育场所及其出现角突和足芽的规律性。 本文还分析了文献上关于恙螨卵壳破裂方式的描述有错误的可能性。 文中附有地里恙螨卵、次卵和卵壳的详图, 并拟就一个较完整的地里恙螨生活史的图解。     

条纹纤恙螨若虫和成虫的形态

条纹纤恙螨Leptotrombidium(Leptotrombidium) striatum 由 Nadchatram 等(1964)首先在东南亚发现。通过调查证实,条纹纤恙螨在我国上海、江苏及安徽数量甚多,而且发现它的季节高峰与已确认的恙虫病媒介小盾纤恙螨 L.(L.) scutellaris(Nagago,1921)相似。为了探索条纹纤恙螨的生物学特性以及与疾病的关系,作者首先对其生活史进行了研究,并首发现了它的若虫和成虫,随即对其形态进行了观察。本文就条纹纤恙螨的若虫和成虫的形态描述如下。     

实验室饲养恙螨的饲料供应

恙螨生活史的观察和传病机制的研究,有赖于实验室内饲养,因此饲养方法非常重要。 实验室饲养恙螨的装置、饲养条件和饲料供应等方面已有很多研究报导,我们参照了文献介绍的方法,作了某些改进,特别是在饲料供应方面。近年来我们     

五种恙螨精胞(真螨目:恙螨科)(恙螨研究 ⅩⅨ)

一、绪 言 与氏阿康恙螨Acomatacarus yosanoi Fukuzumi et Obata,1953的精胞发现及其间接交配试验的结果,促使我们进一步考虑这一现象的普遍性问题。 1957年春季中国人民解放军军事医学科学院获得了大批恙螨幼虫,并在实验室中饲养到达成虫,为了帮助我们了解这一生活史特性,故在同年5月和8月之间把饲养所得的成虫供给我们观察了一个时期,这些成虫是地里恙螨Trombicula deliensis Walch,1923,     

恙螨研究XX.鸡新勋恙螨稚虫和成虫形态的描述

1.本文对鸡新勋恙螨稚虫的形态作了详细的观察,并重新予以补充描述。 2.发现了鸡新勋恙螨的成虫,并作了形态的描述。鸡新勋恙螨的成虫是前人未曾记载过的。 3.新勋恙螨属内成虫的记述以本文为首次。     

肾综合征出血热病毒在恙螨体内增殖的动态观察

取饲养的小盾纤恙螨幼虫和若虫．每20d为一批制成无菌滤液接种Vero-E6细胞测定HFRSV滴度,动态观察HFRSV在螨体内的增殖情况,并用PCR技术检测恙螨幼虫和若虫体内HFRSV－RNA。结果如下：小盾纤恙螨幼虫期除60d一批外,其余各批均在不同时间内测出HFRSV滴度,3批若虫中亦有2批测出HFRSV滴度并检测到HFRSV－RNA。结果为小盾纤恙螨作为HFRS的传播媒介提供了进一步的证据。     

恙螨酯酶同工酶的比较研究

本实验采用聚丙烯酰胺凝胶垂直板型电泳法分析了五种恙螨成虫及微红纤恙螨各虫期酯酶同工酶的变化.在酶谱上,五种恙螨成虫均有区别,酶带数和酶活性强弱均有不同,其差异的大小符合它们现有的种属关系的远近,即与它们所处的分类地位相符.因此在以幼虫为主的恙螨分类中,成虫的同工酶分析可起到辅助和补充的作用.在微红纤恙螨各虫期的酶谱中亦存在差异,酶带数的趋势是从卵至成虫由少渐多.此结果表明酯酶同工酶与个体发育过程中的体内变化有关,可成为指示恙螨个体发育过程中的生化指标之一.     

恙螨子代幼虫群体形态在分类上的应用

采自湖南吉首和福建永定的两群形态相似的纤恙螨在实验室中饲养。将它们1雌1雄进行配对。当幼虫孵出后测量并比较其体各部的测量数,同时将其成虫与地里纤恙螨成虫酯酶同工酶酶谱进行比较。结果表明此两群形态相似的纤恙螨系英帕纤恙螨(Leptotrombidium imphalum)的不同地理亚种,它们形态与福建东部地区恙虫病媒介微红纤恙螨十分相似。恙螨子代幼虫群体形态的测量数系恙螨分类中的主要参数。     

Crystal structure, detonation performance, and thermal stability of a new polynitro cage compound: 2, 4, 6, 8, 10, 12, 13, 14, 15-nonanitro-2, 4, 6, 8, 10, 12, 13, 14, 15-nonaazaheptacyclo [5.5.1.13, 11. 15, 9] pentadecane

A new polynitro cage compound 2, 4, 6, 8, 10, 12, 13, 14, 15-nonanitro-2, 4, 6, 8, 10, 12, 13, 14, 15-nonaazaheptcyclo [5.5.1.1(3,11).1(5,9)] pentadecane (NNNAHP) was designed in the present work. Its molecular structure was optimized at the B3LYP/6-31 G(d,p) level of density functional theory (DFT) and crystal structure was predicted using the Compass and Dreiding force fields and refined by DFT GGA-RPBE method. The obtained crystal structure of NNNAHP belongs to the P-1 space group and the lattice parameters are a = 9.99 ?, b = 10.78 ?, c = 9.99 ?, α = 90.01°, β = 120.01°, γ = 90.00°, and Z = 2, respectively. Based on the optimized crystal structure, the band gap, density of state, thermodynamic properties, infrared spectrum, strain energy, detonation characteristics, and thermal stability were predicted. Calculation results show that NNNAHP has detonation properties close to those of CL-20 and is a high energy density compound with moderate stability.     

Distribution of N,P, K,Ca, Mg,S, Zn,Fe, Mn and Cu in groundnut

Summary Concentration of N, P, K, Ca, Mg and S in summer groundnut crop was higher than in kharif while Zn, Fe, Mn and Cu contents were higher in summer crop. Kernel's N, P and Zn; Leaflet's Ca and Mn; Stem's K and Fe; Root's S and Cu and Petiole's Mg contents were highest. Shell's N, P, K, Mg, S, Zn and Cu; Kernel's Ca, Fe and Mn contents were the least. N, P, K, S, Zn and Cu concentrations decreased linearly as the crop grew. Ca, Mg, Fe and Mn concentrations did not display any distinct pattern. Ca concentration was positively correlated with pod yield in both the seasons.     

Simultaneous measurement of the trace elements Al, As, B, Be, Cd, Co, Cu, Fe, Li, Mn, Mo, Ni, Rb, Se, Sr, and Zn in human serum and their reference ranges by ICP-MS

The goal of this article was to establish reference ranges of the concentration of trace elements in human serum and to compare these results with those reported by other authors. We describe the sample preparation and measurement conditions that allow the rapid, precise, and accurate determination of Al, As, B, Be, Cd, Co, Cu, Fe, Li, Mn, Mo, Ni, Rb, Se, Sr, and Zn in human serum samples (n=110) by inductively coupled plasma-mass spectrometry (ICP-MS). Accuracy and precision were determined by analyzing three reconstituted reference serum samples by comparison with other methods and by the standard addition procedure. The advantages of the ICP-MS method include short time of analysis of the elements mentioned, low detection limit, high precision, and high accuracy. Disadventages include a high risk of contamination due to the presence of some of the elements of interest in the environment, the relatively delicate sample handling, and the high cost of the equipment.     

Reference values for the concentrations of As,Cd, Co,Cr, Cu,Fe, I,Hg, Mn,Mo, Ni,Pb, Se,and Zn in selected human tissues and body fluids

This report attempts to formulate reference ranges of elemental concentrations for 15 trace elements in selected human tissues and body fluids. A set of samples consisting of whole blood, blood serum, urine, milk, liver, and hair were chosen and considered for 15 elements of biological significance: As, Cd, Co, Cr, Cu, F, Fe, I, Hg, Mn, Mo, Ni, Pb, Se, and Zn. The results represent wholly or partially data received from 40 countries of the global regions of Africa, Asia, Europe, North, South, and Central America, Australia, and New Zealand. This survey, even if qualitative, has been useful in demonstrating certain trends of trace-element scenarios around the world. It is of course recognized that both diet and environment exert a strong influence on the distribution pattern of several elements, such as As, Cd, Mn, Pb, Se, and Zn. A limited comparison of the available information on soil status of different countries reflected some interesting associations for elements, such as Mn and Zn. Importantly, this study revealed that only a few countries were in a position to identify a reasonable amount of data on samples requested for this project. Regretably, for a number of countries, any dependable data for even such essential elements as Cu, Fe, and Zn were not available. In view of the nutritional importance of many elements, the time is ripe for concerted efforts by intergovernmental agencies to initiate investigations or commission task forces/projects to generate reliable reference data for selected global regions, which sadly lack data of any kind at present.     

Relations of the Al,B, Ba,Br, Ca,Cl, Cu,Fe, K,Li, Mg,Mn, Na,P, S,Si, Sr,and Zn mass fractions to morphometric parameters in pediatric and nonhyperplastic young adult prostate glands

The variation with age of the 18 trace element mass fractions and some histological characteristics of intact prostate glands of 50 subjects aged 0–30 years was investigated by instrumental neutron activation analysis, inductively coupled plasma atomic emission spectrometry, and a quantitative morphometric analysis. Mean values ± standard error of the mean (M ± SΕΜ) for the mass fractions (in milligrams per kilogram wet tissue) of these trace elements in pre-puberty were: Al 28.5 ± 9.0, B 0.40 ± 0.11, Ba 1.48 ± 0.44, Br 10.5 ± 1.5, Ca 241 ± 30, Cl 3,203 ± 278, Cu 3.51 ± 0.89, Fe 33.7 ± 4.1, K 2,364 ± 145, Li 0.020 ± 0.004, Mg 153 ± 23, Mn 0.46 ± 0.06, Na 2,286 ± 130, P 1,391 ± 100, S 1,698 ± 132, Si 62 ± 11, Sr 0.38 ± 0.08, and Zn 27.6 ± 2.3. During puberty and postpuberty, when there is a significant increase in circulating androgens, the mean values were: Al 7.2 ± 1.4, B 0.21 ± 0.05, Ba 0.25 ± 0.06, Br 5.8 ± 1.0, Ca 433 ± 81, Cl 2,314 ± 201, Cu 1.77 ± 0.13, Fe 20.9 ± 1.6, K 2,585 ± 118, Li 0.0088 ± 0.0014, Mg 232 ± 27, Mn 0.34 ± 0.04, Na 1,875 ± 107, P 1,403 ± 98, S 1,673 ± 73, Si 22.2 ± 3.1, Sr 0.22 ± 0.03, and Zn 93.3 ± 8.9. Mean values (M ± SΕΜ) of percent volumes (%) of the stroma, epithelium and lumen in the prostate before puberty were 73.4 ± 2.6, 20.4 ± 1.7, and 4.45 ± 0.94, respectively, versus 46.5 ± 2.5, 38.5 ± 1.9, and 14.9 ± 1.2 during puberty and postpuberty. This work’s results confirm that the Zn mass fraction in prostate tissue is an androgen-dependent parameter. For the first time it has been demonstrated that the glandular lumen is a main pool of Ca, Mg, and Zn accumulation and that the stroma is a main pool of Al, B, Ba, Br, Cl, Cu, Fe, Mn, Na, and Si accumulation in the normal human prostate, for the age range 0–30 years. It was concluded that the Ca, Mg, and Zn binds tightly within the prostatic fluid, because the volume of glandular lumen reflects the volume of prostatic fluid.     

The Effect of Age and Gender on Al, B, Ba, Ca, Cu, Fe, K, Li, Mg, Mn, Na, P, S, Sr, V, and Zn Contents in Rib Bone of Healthy Humans

The effect of age and gender on major, minor, and trace element contents in the intact rib bone of 80 relatively healthy 15–55-year-old women and men was investigated. Contents or upper limit of contents of 16 chemical elements in the rib bone were determined by inductively coupled plasma atomic emission spectrometry (ICP-AES). Mean values (M?±?SΕΜ) for the mass fraction of Ba, Ca, Cu, Fe, K, Li, Mg, Na, P, S, Sr, and Zn (milligram per kilogram of dry bone) were as follows: 2.54?±?0.16, 171,400?±?4,050, 1.35?±?0.22, 140?±?11, 1,874?±?71, 0.049?±?0.011, 2,139?±?38, 5,378?±?88, 75,140?±?1,660, 1,881?±?51, 291?±?20, and 92.8?±?1.5, respectively. The upper limits of contents of Al, B, Mn, and V were <7.20, <0.65, <0.36, and <0.03, respectively. Statistically significant tendency for the Ca, Mg, and P content to decrease with age was found in the human rib bone, regardless of gender. The mass fraction of Fe in the male rib bone increases with age. It was shown that higher Ca, Mg, Na, P, and Sr mass fractions as well as lower Fe content were typical of female ribs as compared to those in male ribs.     

Potential Compounds for Oral Cancer Treatment: Resveratrol,Nimbolide, Lovastatin,Bortezomib, Vorinostat,Berberine, Pterostilbene,Deguelin, Andrographolide,and Colchicine

Oral cancer is one of the main causes of cancer-related deaths in South-Asian countries. There are very limited treatment options available for oral cancer. Research endeavors focused on discovery and development of novel therapies for oral cancer, is necessary to control the ever rising oral cancer related mortalities. We mined the large pool of compounds from the publicly available compound databases, to identify potential therapeutic compounds for oral cancer. Over 84 million compounds were screened for the possible anti-cancer activity by custom build SVM classifier. The molecular targets of the predicted anti-cancer compounds were mined from reliable sources like experimental bioassays studies associated with the compound, and from protein-compound interaction databases. Therapeutic compounds from DrugBank, and a list of natural anti-cancer compounds derived from literature mining of published studies, were used for building partial least squares regression model. The regression model thus built, was used for the estimation of oral cancer specific weights based on the molecular targets. These weights were used to compute scores for screening the predicted anti-cancer compounds for their potential to treat oral cancer. The list of potential compounds was annotated with corresponding physicochemical properties, cancer specific bioactivity evidences, and literature evidences. In all, 288 compounds with the potential to treat oral cancer were identified in the current study. The majority of the compounds in this list are natural products, which are well-tolerated and have minimal side-effects compared to the synthetic counterparts. Some of the potential therapeutic compounds identified in the current study are resveratrol, nimbolide, lovastatin, bortezomib, vorinostat, berberine, pterostilbene, deguelin, andrographolide, and colchicine.