共查询到20条相似文献,搜索用时 31 毫秒
1.
2.
Gimona M 《Nature reviews. Molecular cell biology》2006,7(1):68-73
The correspondence between biology and linguistics at the level of sequence and lexical inventories, and of structure and syntax, has fuelled attempts to describe genome structure by the rules of formal linguistics. But how can we define protein linguistic rules? And how could compositional semantics improve our understanding of protein organization and functional plasticity? 相似文献
3.
Yaniv Lustig Chaim Wachtel Mark Safro Li Liu Shulamit Michaeli 《Nucleic acids research》2010,38(1):e5
In this study we describe a novel method to investigate the RNA–RNA interactions between a small RNA and its target that we termed ‘RNA walk’. The method is based on UV-induced AMT cross-linking in vivo followed by affinity selection of the hybrid molecules and mapping the intermolecular adducts by RT–PCR or real-time PCR. Domains carrying the cross-linked adducts fail to efficiently amplify by PCR compared with non-cross-linked domains. This method was calibrated and used to study the interaction between a special tRNA-like molecule (sRNA-85) that is part of the trypanosome signal recognition particle (SRP) complex and the ribosome. Four contact sites between sRNA-85 and rRNA were identified by ‘RNA walk’ and were further fine-mapped by primer extension. Two of the contact sites are expected; one contact site mimics the interaction of the mammalian Alu domain of SRP with the ribosome and the other contact sites include a canonical tRNA interaction. The two other cross-linked sites could not be predicted. We propose that ‘RNA walk, is a generic method to map target RNA small RNAs interactions in vivo. 相似文献
4.
Although artificial RNA motifs that can functionally replace the GNRA/receptor interaction, a class of RNA–RNA interacting motifs, were isolated from RNA libraries and used to generate designer RNA structures, receptors for non-GNRA tetraloops have not been found in nature or selected from RNA libraries. In this study, we report successful isolation of a receptor motif interacting with GAAC, a non-GNRA tetraloop, from randomized sequences embedded in a catalytic RNA. Biochemical characterization of the GAAC/receptor interacting motif within three structural contexts showed its binding affinity, selectivity and structural autonomy. The motif has binding affinity comparable with that of a GNRA/receptor, selectivity orthogonal to GNRA/receptors and structural autonomy even in a large RNA context. These features would be advantageous for usage of the motif as a building block for designer RNAs. The isolated motif can also be used as a query sequence to search for unidentified naturally occurring GANC receptor motifs. 相似文献
5.
6.
Burr D 《Current biology : CB》1999,9(3):R90-R92
It has commonly been assumed that the many separate areas of the visual system perform modular analyses, each restricted to a single attribute of the image. A recent paper advocates a radically different approach, where all areas in the hierarchy analyse all attributes of the image to extract perceptually relevant decisions. 相似文献
7.
8.
9.
Xiaomeng Zhang Deng Wu Liqun Chen Xiang Li Jinxurong Yang Dandan Fan Tingting Dong Mingyue Liu Puwen Tan Jintian Xu Ying Yi Yuting Wang Hua Zou Yongfei Hu Kaili Fan Juanjuan Kang Yan Huang Zhengqiang Miao Miaoman Bi Nana Jin Kongning Li Xia Li Jianzhen Xu Dong Wang 《RNA (New York, N.Y.)》2014,20(7):989-993
10.
Food purchases: Impacts from the consumers’ point of view investigated with a modular LCA 总被引:1,自引:0,他引:1
Niels Jungbluth Olaf Tietje Roland W. Scholz 《The International Journal of Life Cycle Assessment》2000,5(3):134-142
The goal of this research work was to assist consumers in considering environmental aspects of food consumption. A simplified,
modular LCA approach has been used to evaluate the impacts from the consumers’ point of view. Comparative LCA’s have been
calculated for five single aspects of decisions: type of agricultural practice, origin, packaging material, type of preservation,
and consumption. The inventory for one module includes the environmental impacts related to one particular product characteristic.
The modular LCA allows one to investigate the trade-offs among different decision parameters. It could be shown that most
of the decision parameters might have an influence on the overall impact of a vegetable product. Greenhouse production and
vegetables transported by air cause the highest surplus environmental impact. For meat products, the agricultural production
determines the overall environmental impact. The total impact for vegetable or meat purchases may vary by a factor of eight
or two-and-a-half. Different suggestions for consumers have been ranked according to the variation of average impacts, due
to a marginal change of behaviour. Avoiding air-transported food products leads to the highest decrease of environmental impacts. 相似文献
11.
Hen1 is an RNA ribose 2′-O-methyltransferase that modifies the 3′ terminal nucleoside of eukaryal small regulatory RNAs. Here, we report that Hen1 homologs are present in bacterial proteomes from eight different phyla. Bacterial Hen1 is encoded by the proximal ORF of a two-gene operon that also encodes polynucleotide kinase-phosphatase (Pnkp), an RNA repair enzyme. Purified recombinant Clostridium thermocellum Hen1 is a homodimer of a 465-amino acid polypeptide. CthHen1 catalyzes methyl transfer from AdoMet to the 3′ terminal nucleoside of an RNA oligonucleotide, but is unreactive with a synonymous DNA oligonucleotide or an RNA with a single 3′-terminal deoxyribose sugar. CthHen1 is optimally active at alkaline pH and dependent on manganese. Activity is inhibited by AdoHcy and abolished by mutations D291A and D316A in the putative AdoMet-binding pocket. The C-terminal fragment, Hen1-(259–465), comprises an autonomous monomeric methyltransferase domain. 相似文献
12.
The primary structure of linkers in a new class of modular α-amylases constitutes a paradigm of the structural basis that allows a polypeptide to remain nonfolded, extended and pliable. Unfolding is mediated through a depletion of hydrophobic residues and an enrichment of hydrophilic residues, amongst which Ser and Thr are over-represented. An extended and flexible conformation is promoted by the sequential arrangement of Pro and Gly, which are the most abundant residues in these linkers. This is complemented by charge repulsion, charge clustering and disulfide-bridged loops. Molecular dynamics simulations suggest the existence of conformational transitions resulting from a transient and localized hydrophobic collapse, arising from the peculiar composition of the linkers. Accordingly, these linkers should not be regarded as fully disordered, but rather as possessing various discrete structural patterns allowing them to fulfill their biological function as a free energy reservoir for concerted motions between structured domains. 相似文献
13.
14.
Genetic recombination is a basic cellular process required for altering genome structure. The RecA protein of Escherichia coli has a central role in homologous recombination, and a eukaryotic protein with similar properties has been discovered in the yeast Saccharomyces cerevisiae. Unexpectedly, this RecA-like protein has additional biochemical activities, and its function may not be restricted to recombination. 相似文献
15.
The crystal structure determination of antibiotic binding sites on the 30S ribosomal subunit and the increasing demand for
developing RNA-based drugs has prompted us to study the direct binding of spectinomycin, vancomycin and bleomycin with yeast
total RNA using Fourier transform infrared (FTIR) spectroscopy. We report that the OH of spectinomycin and the peptide group
of vancomycin can bind to the bases of RNA, which might depend on Mg2+ concentration. Bleomycin on the other hand does not show such a drastic effect on yeast total RNA. This study might help in
developing innovative strategies utilizing RNA molecules to perform a variety of essential biological functions. 相似文献
16.
A cellulose-binding protein of 105 kDa (CBP105) from Cellulomonas flavigena was purified and its gene was cloned. CBP105 is a processive endoglucanase with maximum activity on carboxymethyl cellulose
(CMC) at pH 7.5 and 60°C. Limited proteolysis suggested that CBP105 is composed of one catalytic domain (CD) and two carbohydrate-binding
modules (CBM). The nucleotide sequence of the cbp105 gene (AY729806) indicates that CBP105 is a modular enzyme with a family 9 glycoside hydrolase CD linked to a family 3 CBM,
two fibronectin III-like domains and a family 2 CBM. This structural organization may be responsible for CBP105 processive
CMC degradation. 相似文献
17.
18.
Pillai RS 《RNA (New York, N.Y.)》2005,11(12):1753-1761
19.
20.
RNA interference: a promising approach to antiviral therapy? 总被引:9,自引:0,他引:9