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1.
Dongyeop Kim Irnayuli R. Sitepu Yasuyuki Hashidoko 《Applied and environmental microbiology》2013,79(16):4845-4852
Burkholderia unamae CK43B, a member of the Betaproteobacteria that was isolated from the rhizosphere of a Shorea balangeran sapling in a tropical peat swamp forest, produces neither indole nor extracellular polymeric substances associated with biofilm formation. When cultured in a modified Winogradsky''s medium supplemented with up to 1.7 mM indole, B. unamae CK43B maintains its planktonic state by cell swelling and effectively degrades exogenous indole. However, in medium supplemented with 1.7 mM exogenous indole and 1.0 mM gallic acid, B. unamae CK43B produced extracellular polymeric substances and formed a biofilm. The concentration indicated above of gallic acid alone had no effect on either the growth or the differentiation of B. unamae CK43B cells above a certain concentration threshold, whereas it inhibited indole degradation by B. unamae CK43B to 3-hydroxyindoxyl. In addition, coculture of B. unamae CK43B with indole-producing Escherichia coli in nutrient-rich Luria-Bertani medium supplemented with 1.0 mM gallic acid led to the formation of mixed cell aggregates. The viability and active growth of B. unamae CK43B cells in a coculture system with Escherichia coli were evidenced by fluorescence in situ hybridization. Our data thus suggest that indole facilitates intergenus communication between indole-producing gammaproteobacteria and some indole-degrading bacteria, particularly in gallic acid-rich environments. 相似文献
2.
During the transition from exponential to stationary phase E. coli produces a substantial quantity of the small, aromatic signalling molecule indole. In LB medium the supernatant indole concentration reaches a maximum of 0.5–1 mM. At this concentration indole has been implicated in many processes inducing acid resistance and the modulation of virulence. It has recently been shown that cell-associated indole transiently reaches a very high concentration (approx. 60 mM) during stationary phase entry, presumably because indole is being produced more rapidly than it can leave the cell. It is proposed that this indole pulse inhibits growth and cell division, causing the culture to enter stationary phase before nutrients are completely exhausted, with benefits for survival in long-term stationary phase. This study asks how E. coli cells rapidly upregulate indole production during stationary phase entry and why the indole pulse has a duration of only 10–15 min. We find that at the start of the pulse tryptophanase synthesis is triggered by glucose depletion and that this is correlates with the up-regulation of indole synthesis. The magnitude and duration of the resulting indole pulse are dependent upon the availability of exogenous tryptophan. Indole production stops when all the available tryptophan is depleted and the cell-associated indole equilibrates with the supernatant. 相似文献
3.
《Enzyme and microbial technology》1987,9(8):466-469
By modification of a standard Murashige and Skoog plant tissue culture maintenance medium, a system has been developed for Catharanthus roseus cell suspension cultures in which both growth and indole alkaloid accumulation can occur in a single-stage culture of 14–21 days. Precise optimization of the medium depends upon the cell line under investigation, but the inclusion of lactose as the carbohydrate source, and NAA and kinetin as growth regulators, will generally increase yields of the indole alkaloid catharanthine. Treatment of cells growing in this optimized medium with agents that stimulate the accumulation of secondary metabolites both increases the yield of catharanthine and reduces the time required for production. We believe that this process could be useful for the commercial production of plant secondary metabolites. 相似文献
4.
Plant Growth Substances Produced by Azospirillum brasilense and Their Effect on the Growth of Pearl Millet (Pennisetum americanum L.) 总被引:20,自引:12,他引:8
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Azospirillum brasilense, a nitrogen-fixing bacterium found in the rhizosphere of various grass species, was investigated to establish the effect on plant growth of growth substances produced by the bacteria. Thin-layer chromatography, high-pressure liquid chromatography, and bioassay were used to separate and identify plant growth substances produced by the bacteria in liquid culture. Indole acetic acid and indole lactic acid were produced by A. brasilense from tryptophan. Indole acetic acid production increased with increasing tryptophan concentration from 1 to 100 μg/ml. Indole acetic acid concentration also increased with the age of the culture until bacteria reached the stationary phase. Shaking favored the production of indole acetic acid, especially in a medium containing nitrogen. A small but biologically significant amount of gibberellin was detected in the culture medium. Also at least three cytokinin-like substances, equivalent to about 0.001 μg of kinetin per ml, were present. The morphology of pearl millet roots changed when plants in solution culture were inoculated. The number of lateral roots was increased, and all lateral roots were densely covered with root hairs. Experiments with pure plant hormones showed that gibberellin causes increased production of lateral roots. Cytokinin stimulated root hair formation, but reduced lateral root production and elongation of the main root. Combinations of indole acetic acid, gibberellin, and kinetin produced changes in root morphology of pearl millet similar to those produced by inoculation with A. brasilense. 相似文献
5.
James K. Porter Charles W. Bacon Horace G. Cutler Richard F. Arrendale Joe D. Robbins 《Phytochemistry》1985,24(7):1429-1431
Balansia epichloë, a systemic plant pathogen isolated from Sporobolus poiretii, was shown to produce the plant growth regulators 3-indole acetic acid, 3-indole ethanol, 3-indole acetamide and methyl-3-indole carboxylate when grown on a medium containing tryptophan. When grown on a tryptophan deficient medium 3-substituted indole derivatives were not detected. However, extracts of the medium in lower doses increased and in higher doses inhibited the growth of wheat coleoptiles. 相似文献
6.
《Plant science》1986,47(1):71-76
Evidence is presented that the β-carboline alkaloids norharman and harman are artifacts formed from L-tryptophan. Transfer of Cinchona ledgeriana suspension cultured cells to Zenk's alkaloid production medium (ZAP medium) resulted in cell death. No quinoline or terpenoid indole alkaloids were formed. However, the L-tryptophan present in the production medium was transformed to the β-carboline alkaloids, harman and norharman. It was demonstrated that norharman was also formed in ZAP medium without cells and in ZAP medium containing frost-killed cell material. 相似文献
7.
Axillary shoot proliferation was obtained using explants of Eucalyptus grandis L. juvenile and mature stages on a defined medium. Murashige and Skoog medium (MS) supplemented with benzyladenine (BA), naphthalene acetic acid (NAA) and additional thiamine. Excised shoots were induced to root on a sequence of three media: (1) White's medium containing indoleacetic acid (IAA), NAA and indole butyric acid; (IBA), (2) half-strength MS medium with charcoal and (3) half-strength MS liquid medium. The two types of explants differed in rooting response, with juvenile-derived shoots giving 60% rooting and adult-derived ones only 35%. Thus, the factors limiting cloning of selected trees in vitro are determined to be those controlling rooting of shoots in E. grandis. 相似文献
8.
Balansia epichloë (Weese) and B. henningsiana (Moell.) were isolated from grasses in toxic fescue pastures. B. epichloë, cultured in a synthetic medium, was toxic to chicken embryos. Thin-layer chromatography and ultraviolet absorption data indicated that in submerged culture the fungus produced compounds with the indole or ergoline nucleus. 相似文献
9.
A Two-Dimensional Vibrating Probe Study of Currents around Lateral Roots of Raphanus sativus Developing in Culture 总被引:3,自引:1,他引:2
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A computer-assisted, two-dimensional vibrating probe was used to study the ionic currents around developing lateral roots of Raphanus sativus in vitro. This system allowed us to superimpose current vectors on the video image of the roots. In a young lateral root, current entered the cap, meristematic, and elongation zones and exited the primary root surface close to the base of the lateral root. As the lateral root grew, current began to exit from its basal (cell maturation zone) end. The densities of currents entering the apical portion of the faster-growing lateral roots in a medium lacking indole 3-acetic acid were about twice as large as those entering the apical region of the slower-growing lateral roots in indole 3-acetic acid-supplemented medium. 相似文献
10.
Callus tissues were derived from cotyledon segments of Cucumis melo and Cucumis sativus. Four combinations of growth factors, i.e., naphthalene acetic acid (NAA) plus coconut water (CW); NAA plus kinetin; NAA plus 6-benzylaminopurine (BAP) and indole 3-butyric acid plus BAP, were incorporated in the medium for callus initiation as well as for growth of excised callus in culture for six passages. The proportion of total saturated to unsaturated acids and the ratio of linoleic to linolenic acid was influenced by the change in the type of auxin and cytokinin in the combinations used. A many fold increase of myristic acid was recorded for the indole 3-butyric acid plus BAP combination. 相似文献
11.
Pseudomonas sp. strain ST-200 grew on indole as a sole carbon source. The minimal inhibitory concentration of indole was 0.3 mg/ml for
ST-200. However, ST-200 grew in a persolvent fermentation system containing a large amount of indole (a medium containing
20% by vol. diphenylmethane and 4 mg/ml indole), because most of the indole was partitioned in the organic solvent layer.
When the organism was grown in the medium containing indole at 1 mg/ml in the presence of diphenylmethane, more than 98% of
the indole was consumed after 48 h. Isatic acid (0.4 mg/ml) and isatin (0.03 mg/ml) were produced as the metabolites in the
aqueous medium layer.
Received: September 12, 1996 / Accepted: January 2, 1997 相似文献
12.
Benny Daniel Shaji John E. V. Soniya G. M. Nair 《Journal of plant biochemistry and biotechnology.》1999,8(2):105-107
In vitro clonal multiplication of a valuable medicinal species, Naregamia alata is reported. In shoot tip cultures, maximum multiplication (9 fold/28 days) occurred on MS medium containing 3.1 μM 6-benzylaminopurlne and 8.6 μM gibberellic acid. For the rooting of the shoots, 9.8 μM indole 3-butyric acid was the best. After acclimatization, 90% of the micropropagated plantlets were successfully established in the field. 相似文献
13.
Anja Kastell Monika Schreiner Dietrich Knorr Christian Ulrichs Inga Mewis 《Plant Cell, Tissue and Organ Culture》2018,132(3):561-572
Glucosinolates (GS) are secondary plant metabolites comprising different subgroups with opposing effects on human health. Hairy root cultures (HRC) are potent biotechnological tools allowing the biosynthesis of special substances under defined conditions. HRC of Eruca sativa, a brassicaceaous plant, were used to test different strategies to enhance GS levels and to alter the profile. Additional sulphur supply in the nutrient medium increased especially aliphatic GS by 2.7-fold, but also enhanced indole GS by 1.8-fold. Ethephon as well as jasmonic acid as chemical elicitors enhanced only indole GS levels, whereby especially 4-methoxyindol-3-ylmethyl or 1-methoxyindol-3-ylmethyl GS accumulated. Jasmonic acid was used in combination with pulsed electric field treatment as physical elicitor. Already within 24 h, GS levels doubled in treated HRC compared to the control. For estimation of production potency, the GS levels of HRC were compared to contents of aerial and root parts of E. sativa sprouts. HRC showed a distinct GS profile compared to the parent plant with a higher content of indole GS when compared to sprout roots, but overall lower total GS levels. Furthermore, HRC released GS into the culture medium, which could be enhanced by jasmonic acid and pulsed electric field treatment. This could comprise an efficient strategy for a continuous GS production and mining without solvent extraction. 相似文献
14.
Thomas R. Manney 《Journal of bacteriology》1970,102(2):483-488
Indole-accumulating and indole-utilizing tryptophan synthetase mutants of Saccharomyces cerevisiae were found to form complementing diploids with unusual growth properties. These strains grew at nearly the wild-type exponential rate, but only after an abnormally prolonged lag period of a duration that depended on the initial cell density. The exaggerated lag was reduced by adding low concentrations of indole in the medium. The growth properties were interpreted to be a consequence of the inability of these strains to synthesize tryptophan by the normal mechanism. 相似文献
15.
Extracts from the leaves of 60 plants from 33 families were screened for their ability to metabolize indole. Of these plants, only 11 species were found to cause the rapid disappearance of indole. There is no correlation between the family and indole disappearance. While eight species can degrade indole anaerobically, Tecoma, Mussaenda and Duranta species require oxygen. 相似文献
16.
17.
Convolvulaceous species have been reported to contain several bioactive principles thought to be toxic to livestock including the calystegines, swainsonine, ergot alkaloids, and indole diterpene alkaloids. Swainsonine, ergot alkaloids, and indole diterpene alkaloids are produced by seed transmitted fungal symbionts associated with their respective plant host, while the calystegines are produced by the plant. To date, Ipomoea asarifolia and Ipomoea muelleri represent the only Ipomoea species and members of the Convolvulaceae known to contain indole diterpene alkaloids, however several other Convolvulaceous species are reported to contain ergot alkaloids. To further explore the biodiversity of species that may contain indole diterpenes, we analyzed several Convolvulaceous species (n = 30) for indole diterpene alkaloids, representing four genera, Argyreia, Ipomoea, Stictocardia, and Turbina, that had been previously reported to contain ergot alkaloids. These species were also verified to contain ergot alkaloids and subsequently analyzed for swainsonine. Ergot alkaloids were detected in 18 species representing all four genera screened, indole diterpenes were detected in two Argyreia species and eight Ipomoea species of the 18 that contained ergot alkaloids, and swainsonine was detected in two Ipomoea species. The data suggest a strong association exists between the relationship of the Periglandula species associated with each host and the occurrence of the ergot alkaloids and/or the indole diterpenes reported here. Likewise there appears to be an association between the occurrence of the respective bioactive principle and the genetic relatedness of the respective host plant species. 相似文献
18.
A protocol has been established for rapid, high frequency plant regeneration from protoplasts of the wild tomato species Lycopersicon chilense Dun. Cell suspension cultures were obtained from calli initiated from seedling stem explants. Protoplasts were isolated from cell suspensions by an overnight one-step enzyme digestion, purified by washing in salts solution and cultured in liquid medium. Dilution of liquid medium every 3 days, with medium containing low levels of growth regulators and sucrose, was critical for sustained colony formation. Up to 70% of protoplast-derived calli regenerated shoots when cultured on agar-solidified medium with Murashige & Skoog (1962) salts and vitamins, 2.0 mg l-1 zeatin and 0.1 mg l-1 indole acetic acid for 21 days, followed by transfer to the same medium lacking indole acetic acid.Abbreviations BAP
6-benzylaminopurine
- IAA
indole acetic acid
- IBA
indole butyric acid
- MES-2
(N-morpholino)-ethane sulfonic acid
- NAA
-naphthaleneacetic acid
- 2,4-d
2,4-dichlorophenoxyacetic acid 相似文献
19.
Rubrivivax benzoatilyticus JA2 produces indole derivatives (indoles) from aniline, anthranilate or l-tryptophan. Glucose repressed indole production in R. benzoatilyticus JA2, while malate had no effect. Growth of R. benzoatilyticus JA2 on glucose resulted in decrease in culture pH (6.4) compared with malate (8.4). Growth of R. benzoatilyticus JA2 on sugar carbon sources decreased culture pH (6.4–6.6) and indole production. Further, culture pH of 6.4 repressed the indole production, and pH 8.4 promoted the production irrespective of carbon sources used for growth. Moreover, correlation between indole production and culture pH was observed, where acidic pH inhibited indole production, while alkaline pH promoted the production, suggesting the role of pH in indole production. Tryptophan-catabolizing enzyme activities are significantly high in malate-grown cultures (pH 8.4) compared with that of the glucose (pH 6.4)-grown cultures and corroborated well with indole production, indicating their role in indole production. These results confirm that indole production in R. benzoatilyticus JA2 is pH dependent rather than carbon catabolite repression. 相似文献
20.
Bianca Ortiz da Silva Ana Claudia F. Amaral José Luiz P. Ferreira Laura Jane Moreira Santiago Ricardo P. Louro 《In vitro cellular & developmental biology. Plant》2013,49(3):366-372
Croton floribundus Spreng., a native plant from South America, was utilized for in vitro micropropagation and phytochemical analyses. The effects of the addition of naphthaleneacetic acid and indole butyric acid, on the production of shoots and leaves, as well as volatile constituent production, were determined. The combination of naphthaleneacetic acid and indole butyric acid at a ratio of 1:1 led to the production of the maximum number of leaves and longest shoots after a 60-d subculture period. Analyses of leaf dichloromethane extracts using gas chromatography–mass spectrometry showed that monoterpenes and sesquiterpenes were the main chemical classes present in both in vivo and in vitro conditions. Use of these plant growth regulators in the medium-induced quantitative changes in the major monoterpenes (neral, geranial, limonene, and carvone). In vitro leaf extracts produced compounds such as carvone as well as a large amount of trans-β-farnesene, with the highest production of carvone (16.8%) being produced on medium supplemented with 1.0 mg?L?1 naphthaleneacetic acid. These results suggested the occurrence of biotransformation reactions of limonene and farnesyl cations in culture. 相似文献