Study of the plasma parameters in a high-current pulsed magnetron sputtering system

Results are presented from experimental studies of the current-voltage characteristics and spatial and temporal parameters of the plasma in a high-current pulsed magnetron sputtering system with a 10-cm-diameter plane disk cathode. It is shown that the plasma density in such a system is three orders of magnitude higher than that in conventional dc magnetron discharges and reaches 10¹³ cm⁻³ at a distance of 250 mm from the cathode at a peak discharge current of 500 A. The plasma propagates from the cathode region at a velocity of 1 cm/μs in the axial direction and 0.25 cm/μs in the radial direction. Optical emission spectroscopy shows that the degree of plasma ionization increases severalfold with increasing discharge current, mainly at the expense of the sputtered material.     

Immobilization of glucose oxidase on thin-film gold electrodes produced by magnetron sputtering and their application in an electrochemical biosensor

An electrochemical biosensor is described consisting of a thin-layer gold film electrode prepared by cathodic sputtering using a poly(vinyl chloride) sheet as substrate, with voltammetric behaviour comparable to that of conventional polycrystalline gold electrodes, coated with the hydrolysed copolymer hydroxyethyl methacrylate-co-methyl methacrylate onto which glucose oxidase was immobilized. The mechanical properties of the plastic foil substrate permit easy construction of circular-shaped electrodes which were employed as working electrodes for batch injection analysis. The electrochemical biosensor fabrication is inexpensive and can be used as disposable enzyme sensor for the detection of hydrogen peroxide. The biosensor was tested for the determination of glucose in serum and a good correlation was obtained with the measurement using the electrochemical and the spectrophotometric methods.     

Accumulation of products of ferroelectric target sputtering in the plasma of an RF glow discharge

The zone of macroparticle accumulation was revealed based on laser radiation scattering at copper vapor in the plasma of an RF glow discharge upon sputtering of multicomponent nanosize ferroelectric films. This zone was detected at the interface between the negative glow and the dark cathode space of the glow discharge. It is supposed that these particles are charged products of ferroelectric target sputtering.     

Investigation of the helicon discharge plasma parameters in a hybrid RF plasma system

Results of an experimental study of the helicon discharge plasma parameters in a prototype of a hybrid RF plasma system equipped with a solenoidal antenna are described. It is shown that an increase in the external magnetic field leads to the formation of a plasma column and a shift of the maximum ion current along the discharge axis toward the bottom flange of the system. The shape of the plasma column can be controlled via varying the configuration of the magnetic field.     

Optical studies of plasma inhomogeneities in a high-current pulsed magnetron discharge

Results are presented for experimental studies of the plasma glow in a high-current pulsed magnetron discharge by using a high-speed optical frame camera. It is found that the discharge plasma is inhomogeneous in the azimuthal direction. The plasma bunches rotate with a linear velocity of ∼1 cm/μs in the direction of electron Hall drift, and their number is proportional to the discharge current. Plasma inhomogeneities in the form of plasma jets propagate in the form of plasma jets from the cathode region toward the anode. It is shown analytically that the formation of inhomogeneities is caused by the necessity to transfer high-density electron current across the magnetic field.     

Meiotic chromosomes in an infertile male with an unbalanced Y/13 translocation

Summary An infertile male carrying an unbalanced Y/13 translocation is described. Meiotic studies suggest that the unbalanced translocation does not affect meiotic division.     

Characteristics of the magnetron discharge plasma at large distances from the cathode

The parameters of the magnetron plasma at distances several times larger than the cathode diameter were measured. The plasma temperature and density measured by the probe technique were found to be 1.4 eV and 6 × 10¹⁰ cm⁻³, respectively. The dependences of the plasma density and temperature on the argon flow rate in the course of TiAlN coating deposition were determined. Before deposition of the coating, the substrate was cleaned by ion sputtering at substrate bias voltages higher than 200 V.     

Investigation of lightning ignition characteristics based on an impulse current generator

Lightning strike is an important ignition source of forest fires. Artificial lightning discharge is a method for studying lightning fires. However, there is not enough data on the ignition of combustible materials caused by artificial lightning discharge. Previous studies on lightning ignition have focused on the heating and ignition effects of long continuing current (LCC), but the function of the impulse current that occurs before the LCC has not been taken into account. In this paper, an impulse current generator of 8/20 μs was used to simulate the ignition effect of impulse current on conifer needle beds. Different current waveforms have different ignition characteristics. We compared five kinds of conifer needle beds. The average of the current needed to ignite the needle bed of Larix gmelinii (Ruprecht) Kuzeneva was the smallest, and the average of the breakdown voltage was the smallest for the needle bed of Pinus massoniana Lamb. The total energy input to the conifer needle beds was fitted as a multiple log‐linear regression model. The heating energy proportion value varies with different bulk densities, current amplitudes, and moisture contents. Based on this data, the heating energy of the impulse current transferred to the needles can be predicted. This information in conjunction with previous research on LCC was used to derive a lightning ignition prediction model of the full waveform for conifer needle beds.     

Automated diagnostics of a magnetron discharge plasma based on atomic molecular emission spectra

A software-hardware complex intended for investigating spatial distributions of the plasma spectral emissivity is described. It allows us to record and identify the lines and systems of molecular bands in an automatic mode and to perform computer processing of spectra. Molecular bands of deuterium for different electronic-vibrational-rotational transitions are identified. The excitation temperatures of atomic levels, translational, rotational and vibrational temperatures are estimated for a discharge in a planar magnetron.     

Investigation of ion acceleration in an expanding laser plasma by using a hybrid Boltzmann-Vlasov-Poisson model

The acceleration of ions of different species from a plasma slab under the action of a charge-separation electric field driven by hot and cold electrons is studied by using a hybrid Boltzmann-Vlasov-Poisson model. The obtained spatial and energy distributions of light and heavy ions in different charge states demonstrate that the model can be efficiently used to study the ion composition in a multispecies expanding laser plasma. The regular features of the acceleration of ions of different species are investigated. The formation of compression and rarefaction waves in the halo of light ion impurity, as well as their effect on the energy spectrum of the accelerated ions, is analyzed. An approach is proposed that makes it possible to describe the production of fast ions by laser pulses of a given shape. It is shown that the energy of fast ions can be increased markedly by appropriately shaping the pulse. The effect of heating of the bulk of the cold target electrons on the ion acceleration is discussed.     

Electrical performance of low cost cathodes prepared by plasma sputtering deposition in microbial fuel cells

Microbial fuel cells (MFCs) could potentially be utilized for a variety of applications in the future from biosensors to wastewater treatment. However, the amount of costly platinum (Pt) used as a catalyst should be minimized via innovative deposition methods such as sputtering. In addition, alternative and low-cost catalysts, such as cobalt (Co), should be sought. In this study, ultra low Pt or Co cathodes (0.1 mg cm(-2)) were manufactured by plasma sputtering deposition and scanning electron micrographs revealed nano-clusters of metal catalyst in a porous structure favorable to the three-phase heterogeneous catalytic reaction. When operated in single-chamber air-cathode MFCs, sputtered-Co cathodes generated on average the same power as sputtered-Pt cathodes (0.27 mW cell(-1)) and only 27% less than conventional Pt-ink cathodes with a catalyst load 5 times higher (0.5 mg cm(-2)). Finally, microscopy and molecular analyses showed evidence of biocatalysis activity on metal-free cathodes.     

Operational characteristics of an antibody-immobilized QCM system detecting Salmonella spp

A quartz crystal microbalance (QCM) system detecting Salmonella spp. was developed by an anti-Salmonella antibody immobilization onto one gold surface of a piezoelectric quartz crystal surface with sulfosuccinimidyl 6-[3-(2-pyridyldithio)propionamido]hexanoate (sulfo-LC-SPDP) thiolation. The optimum temperature and pH for the antibody-immobilized sensor were 35 degrees C and 7.2, respectively. The frequency shifts obtained were correlated with the Salmonella concentrations in the range 3.2 x 10(6)-4.8 x 10(8) CFU per ml. The system was quite specific to Salmonella spp. and applicable for repetitive use after a regeneration step employing 1.2 M NaOH. A model sample measurement was done for a market milk spiked with Salmonella typhimurium.     

Global characteristics of an ATON stationary plasma thruster operating with krypton and xenon

The global characteristics of an ATON stationary plasma thruster operating on xenon and krypton are investigated. It is shown that, with krypton, the thrust at the same mass flow rate of the working gas is greater and the efficiency is somewhat lower than those with xenon. An efficiency of ～60% was achieved with krypton for the specific impulse attaining 3000 s. The jet divergence is ～±22° for krypton and ～±11° for xenon.     

MHD stability of a collisionless anisotropic plasma in a system with an internal conductor

A study is made of the MHD stability of a collisionless anisotropic-pressure plasma in a nonparaxial magnetic configuration with an internal conductor in cylindrical geometry. A stability criterion for flutelike modes is obtained, and the families of marginally stable profiles of the longitudinal and transverse plasma pressures are calculated by using the Chew-Goldberger-Low anisotropic MHD equations. Possible marginally stable plasma states are considered with allowance for the expected turbulent relaxation and self-organization processes, on the one hand, and isotropization processes, on the other. A stability criterion for Alfvén modes is also derived in the Chew-Goldberger-Low model.     

Seasonal differences in ram seminal plasma revealed by partition in an aqueous two-phase system

Seminal plasma plays an important role in maturation of spermatozoa through hormonal, enzymatic and surface-modifying events. We have previously shown that adsorption of seminal plasma proteins (SPPs) to the sperm cell surface partially restores the functional characteristics of damaged spermatozoa, reproducing those of live cells. In the present report, we investigate the hypothesis that seasonal differences in seminal plasma could affect its ability to recover membrane integrity of cold-shocked sperm. The effect of seminal plasma proteins, obtained in breeding (bsSPPs) and non-breeding (nbsSPPs) season, on cold-shocked ram spermatozoa previously freed from seminal plasma, was analysed by centrifugal counter-current distribution (CCCD) in an aqueous two-phase system as well as membrane integrity determination by fluorescence markers. Cold-shock treatment greatly lowered cell viability in both breeding and non-breeding season spermatozoa. The cold-shocked sperm viability obtained was approximately 20%. The loss of heterogeneity and the decrease in viability revealed by CCCD analysis was reversed by the addition of increasing amounts of bsSPP, which induced restoration of the surface characteristics of viable-like spermatozoa, as well as an increase in the number of recovered viable sperm. However, this restoring effect was much lower when nbsSPPs were added, even in a sixfold higher concentration than used with bsSPPs. Incubation of cold-shocked cells with both kinds of proteins performed in both seasonal periods, showed that the recovering effect was related to the season when the plasma sample was obtained rather than to the semen season. The addition of bsSPPs to cold-shocked sperm accounted for a nearly 50% reversion for both studied breeding seasons. However, the reversion percentages obtained with nbsSPPs were significantly lower (P<0.05) than those found with bsSPPs in both studied seasonal periods. This different reversion capacity of bsSPPs and nbsSPPs was related to a different protein composition, as revealed by comparative sodium dodecyl sulfate–polyacrylamide gel electrophoresis analysis. The bands of 20, 21, 24, 36 and 67 kDa of the bsSP sample profile decreased in winter–spring SP, and were even less intensely stained in summer SP. Densitometric analysis of the stained gel patterns allows automatic comparison among the separated bands, and revealed an important decrease in the content of several bands. The 21.5 kDa band showed the highest decrease, lowering to 14% in June–August plasma with respect to the value obtained in September–December plasma.     

Investigation of behavior of an enzyme in a biphasic system: soybean lipoxygenase-1

Soybean lipoxygenase-1 (EC 1.13.11.12) reaction with linoleic acid as substrate was used to study the biocatalysis in a biphasic system when the reactants have surface-active properties. The poorly water-soluble substrate was initially dissolved in an apolar solvent (octane). The hydroperoxide produced was water soluble and remained in the aqueous phase (borate buffer). The bioreactor was a modified Lewis cell with a well-defined interfacial area between the two phases. Two phenomena were studied separately: the reactant transfer between the two phases and the biocatalyzed reaction in an aqueous medium. This allowed determination of the transfer and the reaction constants. Substrate transfer was found to be affected by the progress of the reaction, because linoleic acid and the hydroperoxy acid have an influence on the interfacial tension. Inactivation of the biocatalyst at the interface was observed in the bioreactor. These results indicate that it is impossible to analyze the system behavior with the method proposed in the literature, which is based on the sequential study of the substrate transfer to the aqueous phase and its biocatalysis by lipoxygenase. The interaction between transfer phenomena and reaction kinetics was studied in the biphasic system. The kinetics were different from those obtained in the aqueous medium. Catalysis and transfer influence each other reciprocally. In this compartmentalized system, cooperativity phenomena were obtained using a nonallosteric enzyme. The evolution of the system was modeled (Runge-Kutta algorithm). The curves obtained were very close to those determined experimentally.     

The development of the plasma membrane reticular system in the fat body of an insect

Several insect tissues have plasma membranes that are folded inwards to make a subsurface reticulum on faces that are exposed to hemolymph. The infolds have been called plasma membrane reticular systems (RSs) to distinguish them from the somewhat similar structures found in transporting epithelia. They are characterized by having negative charges on the plasma membranes of the entranceways and by the concentration of some hemolymph proteins in their lymph spaces. Their formation and loss in the fat body has been studied by scanning electron microscopy during the fifth stadium of Calpodes ethlius (Lepidoptera, Hesperiidae). Fat body cells begin the fifth stadium arranged in ribbons with the cells linked together by a fringe of processes. In the first stage many more processes form. These partially fuse together in the second stage, leaving a subsurface reticulum connected by narrow entrances to the lateral cell faces and the face below the basal lamina. Both the cell processes and the reticular systems that they enclose are usually axially orientated. The completed RS persists for the second half of the intermoult devoted to larval syntheses when the concentration of hemolymph proteins rises. After protein sequestration prior to pupation the RS is lost and the fat body returns to being a tissue of rounded cells linked by a few enmeshed processes.     

Investigation of the decolorization efficiency of two pin-to-plate corona discharge plasma system for industrial wastewater treatment

In this article, a dual pin-to-plate high-voltage corona discharge system is introduced to study experimentally the gap distance, the contact time, the effect of pin and plate materials, the thickness of ground plate and the conductivity on the amount of Acid Blue 25 dye color removal efficiency from polluted water. A study for the optimum air gap distance between dual pin and surface of Acid Blue 25 dye solution is carried out using 3D-EM simulator to find maximum electric field intensity at the tip of both pins. The outcomes display that the best gap for corona discharge is approximately 5 mm for 15-kV source. This separation is constant during the study of other factors. In addition, an investigation of the essential reactive species responsible for oxidation of the dye organic compounds (O₃ in air discharge, O₃ in water, and H₂O₂) during the experimental time is conducted. Three various materials such as: stainless steel, copper and aluminum are used for pins and plate. The maximum color removal efficiencies of Acid Blue 25 dyes are 99.03, 82.04, and 90.78% after treatment time 15 min for stainless steel, copper, and aluminum, respectively. Measurement results for the impact of thickness of an aluminum ground plate on color removal competence show color removal efficiencies of 86.3, 90.78, and 98.06% after treatment time 15 min for thicknesses of 2, 0.5, and 0.1 mm, respectively. The increasing of the solution conductivity leads to the reduction of decolorization efficiency. A kinetic model is used to define the performance of corona discharge system. The models of pseudo-zero-order, pseudo-first-order, and pseudo-second-order reaction kinetics are utilized to investigate the decolorization of Acid Blue 25 dye. The rate of degradation of Acid Blue 25 dye follows the pseudo-first-order kinetics in the dye concentration.