共查询到20条相似文献,搜索用时 31 毫秒
1.
D H Schlesinger H D Niall D Wilson 《Biochemical and biophysical research communications》1974,61(1):282-289
The amino acid sequence of UDP-galactose 4-epimerase has been determined through the amino-terminal 28-amino acid residues using an automated protein sequenator. Alignment of UDP-galactose operon messenger RNA and the amino acid sequence of epimerase demonstrates that the first 26 bases in the mRNA are transcribed but do not take part in translation of epimerase. 相似文献
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C W Adams R P Lawther G W Hatfield 《Biochemical and biophysical research communications》1979,89(2):650-658
Transaminase B of K12 was purified to apparent homogeneity as measured by SDS acrylamide gel electrophoresis, immunoelectrophoresis, and amino terminal sequence analysis. The valine- and isoleucine-α-ketoglutarate dependent transaminase activities of pure enzyme as well as crude extracts were characterized by immunologic and kinetic methods. The data disprove the existence of a separate valine-α-ketoglutarate transaminase within the operon. 相似文献
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The amino acid double labeling technique was used to identify and localize membrane-bound lactose operon proteins in . Both the “M” protein, thought to be the gene product, and a polypeptide of MW ~15,000 appeared in the membrane following operon induction. The amounts of these two proteins were approximately equal.The inner and outer membrane layers of the cell envelope were separated by sucrose density gradient centrifugation or by selective solubilization of inner membranes with the detergent Sarkosyl. When gentle lysis conditions were employed to prepare membrane vesicles, both induced proteins fractionated with the inner membrane. However, the “M” protein was more easily randomized in the envelope structure by sonication than the 15,000 dalton component or an inner membrane marker enzyme. 相似文献
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M Tanaka M Haniu K T Yasunobu 《Biochemical and biophysical research communications》1977,76(4):1014-1019
The amino acid sequence of the 11.6 K dalton heme subunit of bovine heart cytochrome oxidase has been completed and is presented here. The sequence investigation has established the positions in the protein of all the possible heme ligands, namely cysteine, methionine, histidine and lysine residues. However, the isolation conditions may have caused the heme to migrate from its original site or the heme is caged by peptides as pointed out in Reference 6. The sequence of the heme subunit and the β-chain of hemoglobin shows homology. It is possible that these two proteins have arisen from a common ancestor in the distant past. 相似文献
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Amino terminal amino acid sequence of the major polypeptide subunit of Torpedo californica acetylcholine receptor 总被引:2,自引:0,他引:2
M W Hunkapiller C D Strader L Hood M A Raftery 《Biochemical and biophysical research communications》1979,91(1):164-169
The amino terminal sequence of the 40,000 dalton polypeptide subunit of acetylcholine receptor has been determined for twenty-five cycles using automatic microsequencing procedures. The results demonstrate a unique polypeptide sequence for this receptor subunit and quantitation of amino acid recoveries shows that no significant amounts of polypeptides with blocked amino terminii are present. 相似文献
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G Fauque M Bruschi J Le Gall 《Biochemical and biophysical research communications》1979,86(4):1020-1029
A new c-type cytochrome containing a single heme group, cytochrome c553(550) has been purified from (Norway strain) and some of its properties have been investigated. It has an isoelectric point of 6.6 and a higher redox potential than cytochrome c3 isolated from the same bacteria. Its molecular weight was estimated to be 9,200 by gel filtration. The main absorption peaks are at 553, 522.5 and 417 nm in the reduced form and at 690, 529, 411, 357 and 280 nm in the oxidized form. The asymmetric α band of the reduced state is similar to the one reported for socalled “split α” cytochromes c. The cytochrome contains 86 amino acid residues with 5 methionine, two cysteine and two histidine residues. The N terminal sequence of Norway cytochrome c553(550) presents no evident homology with that of Hildenborough cytochrome c553. 相似文献
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Bianca M. Conti-Tronconi Michael W. Hunkapiller Jon M. Lindstrom Michael A. Raftery 《Biochemical and biophysical research communications》1982,106(2):312-318
The amino terminal amino acid sequence of the 41,000 dalton subunit of acetylcholine receptor has been determined for 35 cycles by automated sequencing procedures. Comparison of the unique polypeptide sequence obtained for this molecule with that of the major subunit of acetylcholine receptor reveals extensive primary structural homology between the two proteins. 相似文献
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F. Guerlesquin G. Bovier-Lapierre M. Bruschi 《Biochemical and biophysical research communications》1982,105(2):530-538
An acidic cytochrome c (Pi = 4.8) has been purified from Norway. Its molecular weight was estimated to be 26,000 but a monomeric form of 13,500 molecular weight has been obtained. The comparison of its amino acid composition and N terminal sequence has characterized this cytochrome as a new cytochrome, different from cytochrome c3 (Mr 13,000) and cytochrome c553(550) studied in the same organism. Its optical spectrum was similar to cytochrome c3 (Mr 13,000) accordingly it has 4 haems per subunit. The absence of absorption at 695 nm indicates that two histidine residues are implicated as fifth and sixth ligand for haem iron. This new cytochrome is homologous to the cytochrome C3 (Mr 26,000) previously described for and . 相似文献
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Nucleotide sequence of the genes coding for alpha, beta and gamma subunits of the proton-translocating ATPase of Escherichia coli 总被引:12,自引:0,他引:12
H Kanazawa T Kayano K Mabuchi M Futai 《Biochemical and biophysical research communications》1981,103(2):604-612
A nucleotide sequence of 2328 base pairs comprising a portion of the gene cluster for the proton-translocating ATPase of was determined. The sequence covers most of the gene for α subunit, the entire gene for γ subunit and the amino terminal portion of the gene for β subunit, along with the flanking regions of these genes. The amino acid sequences of these subunits deduced from the DNA sequences indicate that the α and γ subunits have 513 and 287 amino acid residues, respectively. A possible secondary structure for each subunit was estimated from the inferred primary structure. The intercistronic regions between the genes for α and γ and between γ and β are 49 and 26 base pairs, respectively. The significance of codon usage in these genes is discussed in correlation with their expression. 相似文献
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DNA replication in a polymerase I deficient mutant and the identification of DNA polymerases II and 3 in Bacillus subtilis 总被引:6,自引:0,他引:6
A T Ganesan C O Yehle C C Yu 《Biochemical and biophysical research communications》1973,50(1):155-163
The partial amino acid sequences at the amino terminal of prothrombin and the intermediates of activation have been determined. These data indicate that the products of the first step of activation, whether derived from the action of factor Xa or thrombin, are identical. The data also show that the activation of prothrombin proceeds by the sequential cleavage of the amino terminal region of prothrombin and the intermediates, and confirm the mechanism of prothrombin activation as: NH2-Prothrombin-COOH NH2-Intermediate 3 + Intermediate 1-COOH; NH2-Intermediate 1-COOH NH2-Intermediate 4 + Intermediate 2-COOH; NH2-Intermediate 2-COOH NH2-A chain α-thrombin -S-S-B chain α-thrombin-COOH.Previous reports from this laboratory have demonstrated that the activation of prothrombin proceeds through several single-chain intermediates prior to the appearance of thrombin activity. (1) Subsequent studies have sequence of the prothrombin molecule can be deduced from the sequences of its activation intermediates and we are continuing our studies toward this goal. 相似文献
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H. Engelberg-Kulka L. Dekel M. Israeli-Reches 《Biochemical and biophysical research communications》1981,98(4):1008-1015
The regulation of the synthesis of operon enzymes was studied in streptomycin-resistant mutants temperature-sensitive for UGA suppression by normal tRNATrp. Our mutants carry a allele that when transferred to a different genetic background causes repression of trp operon enzyme synthesis at both low (35°C) and high (42°C) temperatures; however, in our mutants with an excess of tryptophan and at increased temperatures enzyme synthesis is derepressed. Based on our results and the sequence data of the R gene [Singleton et al. (1980) Nucleic Acids Res., 8, 1551–1560], we offer a model for the involvement of the limited misreading of UGA codons by normal charged tRNATrp in the autogenous regulation of the R gene expression. The UGA readthrough process may be a regulatory amplifier of the effect of tryptophan starvation. 相似文献
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Stimulation by interferon of induction of differentiation of human promyelocytic leukemia cells 总被引:3,自引:0,他引:3
F1-ATPase was isolated from yeast . The constituent subunits 1 and 2 were purified by gel permeation chromatography, and their amino acid compositions determined. Both subunits have a similar composition except for cystine, methionine, leucine, histidine, and tryptophan. When F1 is treated for three hours with 5′-p-[3H]fluorosulfonylbenzoyl adenosine in dimethylsulfoxide, 90% of the activity is lost. Disc gel electrophoresis of the modified complex showed that over 90% of the label was associated with subunit 2. A labelled peptide from a digest of subunit 2 was isolated and sequenced. It had the following amino acid sequence: His-Try1-Asp-Val-Ala-Ser-Lys-Val-Gln-Glu, whereby Tyr1 is the modified amino acid residue. This sequence shows homology to other sequences obtained from maize, beef heart, and F1-ATPases. 相似文献
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J Morland N E Huseby M Sjoblom J H Stromme 《Biochemical and biophysical research communications》1977,77(3):1060-1066
Chicken liver microsomes contain an albumin having an isoelectric point approximately 0.2 pH unit in excess of that of chicken serum albumin. Although the serum protein is also present in microsomes, only the basic albumin there becomes labelled and undergoes turnover . Sequence analysis of the purified basic microsomal albumin indicates that the first twelve residues are: . The data suggest that the octapeptide (underlined) is attached to the amino terminus of chicken serum albumin (the last four residues). The amino terminal sequence of the serum albumin precursor in chicken liver is thus markedly different from that of the rat and bovine proalbumins. 相似文献
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E L Kline V Bankaitis C S Brown D Montefiori 《Biochemical and biophysical research communications》1979,87(2):566-574
The ability of imidazole acetic acid (IA) to substitute for cAMP was demonstrated by use of a series of strains carrying a lesion in the structural gene. The substitution of IA for cAMP was specific for the L-arabinose operon in that this compound was ineffective in substituting for cAMP in the lactose or maltose catabolic systems. The cAMP receptor protein (CRP) and the gene product were necessary for the IA mediated induction of the L-arabinose system. 相似文献
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Amino and carboxy terminal sequences of the DNA-binding protein HU from a cyanobacterium have been determined. The partial amino acid sequence of the cyanobacterial protein is compared to that of the corresponding protein from . A high degree of similarity in primary structure is detected. The results are interpreted in terms of the large evolutionary distance between and cyanobacteria to suggest that the protein HU is, like eukaryotic histones, highly conserved in primary structure. 相似文献
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Proline inhibition of pyrroline-5-carboxylate reductase: differences in enzymes obtained from animal and tissue culture sources 总被引:3,自引:0,他引:3
D Valle S J Downing J M Phang 《Biochemical and biophysical research communications》1973,54(4):1418-1424
The complete amino acid sequence for the 148 amino acid flavodoxin from is presented. This is the first flavoenzyme for which both the complete amino acid sequence and a 2.5 Å resolution x-ray diffraction structure are now known. The position of some important residues in the binding of FMN are given. The D. vulgaris sequence is compared with other published flavodoxin sequences. 相似文献