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1.
Phytohormonal regulation of S-adenosylmethionine synthetase and S-adenosylmethionine levels in dwarf pea epicotyls. 总被引:1,自引:0,他引:1
A significant stimulation (2- to 2.5-fold) of AdoMet synthetase was witnessed in glibberellicd acid (GA3, 1 microM)-treated epicotyls of the dwarf pea (Pisum sativum). This was accompanied by a 2.4-fold increase in the endogenous pool of S-adenosylmethionine. Both abscisic acid (10 microM) and cycloheximide (20 micrograms/ml) inhibited the GA3-mediated enhancement of AdoMet synthetase activity. Three isozymes of AdoMet synthetase were detected in GA3-treated epicotyls, whereas a single activity peak was observed in controls. Thus, GA3 seems to control the induction of two new isozymes of AdoMet synthetase in the dwarf pea. By contrast, the tall pea exhibited three isozymes of AdoMet synthetase even in the absence of GA3 treatment. High concentration of L-methionine (2 mM) mimicked the GA3-elicited induction of two new isozymes of AdoMet synthetase in dwarf pea epicotyls. 相似文献
2.
Yoshikawa H Ichiki Y Sakakibara KD Tamura H Suiko M 《Bioscience, biotechnology, and biochemistry》2002,66(4):840-846
Lunularic acid (LA) inhibited not only the germination and the growth of cress and lettuce at 1 mM but also the gibberellic acid (GA3)-induced alpha-amylase induction in embryoless barley seeds at 120 microM, which was recognized as a specific activity of abscisic acid (ABA). Moreover LA and ABA equally inhibited the growth of Lunularia cruciata A18 strain callus at 40 and 120 microM. A computational analysis revealed that the stable conformers of LA could be superimposed on the stable ABA conformers. In addition, the antibody raised against the conjugate of C1-ABA-bovine serum albumin (ABA-BSA) reacted with LA-horse-radish peroxidase (LA-HRP) conjugate as well as ABA-HRP conjugate, apparently. These results can explain why LA has ABA-like activity in higher plants. Moreover the results suggest that LA and ABA bind to the same receptor in higher plants. 相似文献
3.
Julie A. T. Buchheim Len F. Burkhart Martin M. Meyer Jr. 《Plant Cell, Tissue and Organ Culture》1994,36(1):35-43
Epicotyl dormancy was broken in cultured peony (Paeonia lactiflora Pall.) embryos after topical application of agarose gels containing gibberellic acid, with optimum growth at 1.5 mM gibberellic acid. Addition of 100 M abscisic acid to the medium resulted in complete inhibition of gibberellic acid-stimulated promotion of dormant epicotyls. Epicotyl dormancy was also broken in embryos by culture on media containing 1 or 10 M benzylaminopurine. A highly significant increase in leaf number occurred when embryos were both cultured on medium containing benzylaminopurine and treated topically with gibberellic acid. Anatomical and morphological studies indicated that the increase in shoot growth was due to the development and growth of 1) buds formed at the cotyledonary node, 2) axillary buds, and 3) adventitious meristems originating from subepidermal parenchymatous tissue.Abbreviations ABA
abscisic acid
- BA
N6-benzylaminopurine
- DMSO
dimethyl sulfoxide
- GA3
gibberellic acid
- LS
Linsmaier and Skoog 相似文献
4.
Reversal of induced dormancy in lettuce by ethylene, kinetin, and gibberellic Acid 总被引:4,自引:2,他引:2
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The germination of lettuce seeds (Lactuca sativa L., cv. Premier Great Lakes) was significantly inhibited by high temperature (32 C), 0.1 mM abscisic acid or 0.4 M mannitol. Ethylene (16 μl/1 of air) partially reversed the dormancy induced by all three inhibitors but only in the presence of 1 mM gibberellic acid (GA) or light. Neither ethylene plus GA nor ethylene plus light were able to promote germination when thermal inhibition was imposed at 36 C. Addition of 0.01 mM kinetin to the ethylene plus GA or light reversed thermodormancy at 36 C. The dormancy imposed by abscisic acid was also reversed by kinetin. Kinetin was unable to reverse the osmotic dormancy imposed by mannitol. The reversal of osmotic dormancy by ethylene or ethylene plus GA was actually inhibited by kinetin but only in the light. Kinetin apparently stimulates cotyledonary growth in the presence of light, and this growth may compete for certain metabolites critical to radicle growth and subsequent germination. Kinetin and ethylene, as demonstrated primarily in the thermodormancy at 36 C and in osmotic dormancy, appear to regulate a common event(s) leading to germination but through mechanisms unique to each respective growth regulator. The regulation of germination by ethylene is absolutely dependent upon an interaction with GA and/or light. 相似文献
5.
Somatic embryogenesis was induced in embryogenic calli of Spinacia oleracea L., on a Murashige and Skoog (1962) medium, containing
4.6 μM kinetin as the sole growth regulator. Abscisic acid, gibberellic acid, and indole-3-acetic acid were supplemented to
kinetin-containing medium and their effects on the initiation of somatic embryos was studied. Abscisic acid at a particular
concentration (4 μM) dramatically increased the number of embryos per g fresh weight of callus, while both gibberellic acid
and indole-3-acetic acid suppressed the embryo initiation. It is suggested that the promoting effect of abscisic acid on the
embryo initiation may be explained as a stress response of the tissue. The relative number of globular embryos vs. the embryos
in heart/torpedo and cotyledonary stages was increased at 4 μM abscisic acid and at all gibberellic acid concentrations (0.3–10
μM). In contrast, the ratio of globular to polar embryos was lower than in controls at 1 μM abscisic acid and indole-3-acetic
acid (1 and 10 μM). The effects of growth regulators on the ratio of globular to polar embryos indicate that they interfere
with the normal distribution of cell division and cell expansion during early embryogenesis.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
6.
Halloin JM 《Plant physiology》1976,57(3):454-455
Germination of cottonseed (Gossypium hirsutum L.) was inhibited by abscisic acid. Inhibition was greater when seeds were soaked in abscisic acid for 5 hours and dried prior to germination than when abscisic acid was applied in the germination medium. (2-Chloroethyl)phosphonic acid, gibberellic acid, and kinetin partially overcame the inhibitory action of abscisic acid. Combinations of (2-chloroethyl)phosphonic acid with gibberellic acid or kinetin were more effective than the individual substances. Germination also was partially restored by removal of seed coats. Fusicoccin completely restored germination of abscisic acidtreated seeds. 相似文献
7.
Effect of phytohormones on fiber initiation of cotton ovule 总被引:1,自引:0,他引:1
Haiping Zhang Mingyan Shao Zhijun Qiao Shuna Yuan Xuede Wang Shuijin Hua 《Acta Physiologiae Plantarum》2009,31(5):979-986
In order to study the effect of phytohormones on cotton fiber initiation, contents of four endogenous phytohormones and activities
of four related enzymes in ovules (in vivo) of a fuzzless–lintless mutant (fl) and its wild-type (FL) line were measured from
4 days before anthesis (day −4) to 4 days after anthesis (day 4). The results showed that contents of indole-3-acetic acid,
gibberellic acid (GA), and zeatin riboside in fl ovules were lower than those in FL ovules. Therefore, indole-3-acetic acid,
GA, and zeatin riboside were thought to be the promoters of fiber initiation. Although abscisic acid (ABA) content in fl ovule
was slightly higher than that in FL ovule on day 0, which might imply that ABA inhibited fiber initiation. Fiber initiation
could also be influenced by enzyme through regulating synthesis and degradation of related phytohormones since fl ovules were
significantly higher in activities of indole-3-acetic acid oxidase, cytokinin oxidase and peroxidase, but lower in activity
of tryptophan synthetase than those in FL ovules. To test the above hypothesis, exogenous plant growth regulators were also
applied for the culture of ovules from fl and FL in vitro. When no regulators were added, no fiber was induced on fl ovule,
but a few fibers were induced in FL ovule. Higher total fiber units (TFU) were observed when indole-3-acetic acid and gibberellic
acid (GA3) were applied either separately or in combination to media. TFU did not increased with zeatin riboside alone, but the highest
TFU was achieved when zeatin riboside was applied together with indole-3 acetic acid and GA3, which implied that fiber initiation could be promoted by them as additive. 相似文献
8.
The objective of this research was to determine if gibberellic acid (GA) and/or abscisic acid (ABA) are necessary for graviresponsiveness by primary roots of Zea mays. To accomplish this objective we measured the growth and graviresponsiveness of primary roots of seedlings in which the synthesis of ABA and GA was inhibited collectively and individually by genetic and chemical means. Roots of seedlings treated with Fluridone (an inhibitor of ABA biosynthesis) and Ancymidol (an inhibitor of GA biosynthesis) were characterized by slower growth rates but not significantly different gravicultures as compared to untreated controls. Gravicurvatures of primary roots of d-5 mutants (having undetectable levels of GA) and vp-9 mutants (having undectable levels of ABA) were not significantly different from those of wild-type seedlings. Roots of seedlings in which the biosynthesis of ABA and GA was collectively inhibited were characterized by gravicurvatures not significantly different for those of controls. These results (1) indicate that drastic reductions in the amount of ABA and GA in Z. mays seedlings do not significantly alter root graviresponsiveness, (2) suggest that neither ABA nor GA is necessary for root gravicurvature, and (3) indicate that root gravicurvature is not necessarily proportional to root elongation. 相似文献
9.
Ladyzhenskaya EP 《Membrane & cell biology》2000,13(5):617-624
Effects of phytohormones gibberellic acid (GA) and abscisic acid (ABA) on the ATP-dependent transmembrane transport of protons were studied in plasma membrane vesicles (PMVs) from non-dormant potato tubers. The uptake of H+ into PMVs was assessed by the fluorescence quenching of acridine orange (AO) after the addition of ATP to the incubation medium. Addition of ATP to the incubation medium led to the instantaneous rise of the AO fluorescence intensity followed by its decrease. The fluorescence quenching was not observed in the presence of either protonophore CCCP or inhibitors of the membrane-bound H+-ATPase. It is concluded that the ATP-induced quenching of the AO fluorescence resulted from the accumulation of protons in PMVs due to the function of the plasma membrane-bound H+-ATPase. Depending on their concentrations, GA and ABA either inhibited or stimulated the ATP-driven H+ translocation across the vesicle membrane. The growth-stimulating hormone GA at concentrations of 10(-9)-10(-5) M increased the initial rate of the fluorescence quenching, whereas 10(-4) M GA slightly inhibited the H+ translocation. The growth inhibitor ABA at a concentration of 10(-9) M slightly increased the rate of the proton accumulation in PMVs; at higher concentrations (10(-8)-10(-4) M), ABA inhibited the H+ translocation. Acetic acid, which has pK similar to pK of GA and ABA, did not influence the ATP-dependent H+ accumulation in PMVs, suggesting the hormone-specific action of GA and ABA on the H+-ATPase activity. In the presence of DCC, which completely inhibited the accumulation of H+, GA and ABA did not affect the passive proton efflux from PMVs. It is proposed that the mechanisms of the regulatory effects of phytohormones may involve modification of H+-ATPase activity leading to changes in the electrochemical gradient of H+ across the plasma membrane. 相似文献
10.
Somatic embryogenesis was induced in hypocotyls of Digitalis obscura using indoleacetic acid or 2,4-dichlorophenoxyacetic acid with different culture and subculture conditions. Indoleacetic acid-induced embryogenic cultures were used to investigate the effects of amino acids, polyamines and growth regulators on embryo differentiation and maturation. Supplementation of the media with amino acids, polyamines or abscisic acid did not influence or had an adverse effect on embryogenic response. Gibberellic acid at 1.4 M in either culture (30 days) or subculture medium was effective in promoting both differentiation and normal embryo development. The efficiency of somatic embryogenesis was greatly enhanced when isolated indoleacetic acid-induced proembryogenic masses were subcultured in liquid medium with reduced auxin content.Abbreviations ABA
abscisic acid
- 2,4-D
2,4-dichlorophenoxyacetic acid
- GA
gibberellic acid
- IAA
indoleacetic acid
- Ptr
putrescine
- Spd
spermidine
- Spn
spermine 相似文献
11.
The role of gibberellic acid (GA) in differentiation and secondary cell-wall deposition of fibre cells of spring wheat (Triticum aestivum) culms was studied using applications of GA and chlormequat (a GA biosynthesis inhibitor). In certain genotypes, higher GA levels may increase the number of cortical fibre cell files by changing cell fate from parenchyma to fibre, and induce thicker secondary cell-walls. 相似文献
12.
Abscisic acid in the thermoinhibition of lettuce seed germination and enhancement of its catabolism by gibberellin 总被引:11,自引:0,他引:11
Gonai T Kawahara S Tougou M Satoh S Hashiba T Hirai N Kawaide H Kamiya Y Yoshioka T 《Journal of experimental botany》2004,55(394):111-118
Germination of lettuce (Lactuca sativa L. cv. 'Grand Rapids') seeds was inhibited at high temperatures (thermoinhibition). Thermoinhibition at 28 degrees C was prevented by the application of fluridone, an inhibitor of abscisic acid (ABA) biosynthesis. At 33 degrees C, the sensitivity of the seeds to ABA increased, and fluridone on its own was no longer effective. However, a combined application of fluridone and gibberellic acid (GA3) was able to restore the germination. Exogenous GA3 lowered endogenous ABA content in the seeds, enhancing catabolism of ABA and export of the catabolites from the intact seeds. The fluridone application also decreased the ABA content. Consequently, the combined application of fluridone and GA3 decreased the ABA content to a sufficiently low level to allow germination at 33 degrees C. There was no significant temperature-dependent change in endogenous GA1 contents. It is concluded that ABA is an important factor in the regulation of thermoinhibition of lettuce seed germination, and that GA affects the temperature responsiveness of the seeds through ABA metabolism. 相似文献
13.
Summary The activities of phenylalanine ammonia lyase and caffeate-0-methyl transferase in bean callus tissue, during differentiation, were induced in a coordinated manner over a period of 21 days. Neither enzyme activity could be induced to increase when differentiation was repressed by the addition of abscisic acid to the induction medium. The formation of soluble phenols was also increased during the induction of differentiation but it was also increased by the transfer to induction medium of an old callus which could no longer differentiate. PAL activity was more directly correlated with xylogenesis and nodule induction than with the formation of soluble phenols. Peroxidase activity in the callus tissue was always high but the level decreased during the induction of differentiation. A new isoenzyme of peroxidase was induced during callus formation and this was probably a response to the presence of auxins in the medium used to grow the callus. 相似文献
14.
Salinity is one of the abiotic stresses that limits the
growth and productivity of many crops. A possible survival strategy
for plant under saline conditions is to use compounds that could
minimize the harmful effects of salt stress on the plant development.
The objective of the presented study was to investigate the effect
of exogenous ascorbic acid (ASA) with or without gibberellic acid
(GA3) on key growth and biochemical parameters in two petunia
cultivars ‘Prism Rose’ and ‘Prism White’ under saline (150 mM
NaCl) and non-saline in vitro condition. Nodal cutting with an
axillary buds were used as explants. Application of 1 mM ascorbic
acid with or without 0.05 mM gibberellic acid into the MS medium
stimulated the length of shoots and the number of new shoots of
‘Prism Rose’; whereas, it decreased the root length and the number
of roots of both ‘Prism Rose’ and ‘Prism White’ under non-saline
condition. The addition of ascorbic acid with or without gibberellic
acid into the MS medium under saline condition, increased the
length of plants and the number of new shoots, but did not affect
their root number and length. NaCl treatments increased the
proline content and lipid peroxidation which was indicated by the
accumulation of malondialdehyde (MDA). The study revealed
a correlation between chlorophylls a and b content and the leaf
pigmentation intensity – parameter a*. Addition of 1 mM ascorbic
acid with 0.05 mM gibberellic acid into the MS medium plays
a protective role in salinity tolerance by improving the shoot
growth and the development as well as increasing the activities
of the antioxidant enzymes and other antioxidant substances. 相似文献
15.
W E Donaldson 《Chemico-biological interactions》1975,11(5):343-350
(1) Subcutaneous or intra-abdominal injections of 8 mg of HgCl2/100 g body weight markedly depressed hepatic fatty acid synthetase activity of chicks at 1 h post-injection. The depression occurred despite the fact that the chicks continued to eat up until the time they were killed. Under these same conditions, the hepatic activity of acetyl-CoA carboxylase (EC 6.4.1.2) was not affected by HgCl2, while the activity of the mitochondrial system of fatty acid elongation was stimulated. (2) When 2-mercaptoethanol was included in the incubation medium for a highly purified preparation of fatty acid synthetase, 500 muM HgCl2 was required to show definite inhibition of the enzyme. When 2-mercaptoethanol was omitted, 50 muM HgCl2 was inhibitory and 100 muM HgCl2 abolished enzyme activity. (3) 2 mM dithiothreitol completely protected the purified fatty acid synthetase preparation from inhibition by 100 muM HgCl2. When dithiothreitol was added after the addition of enzyme to the mercury-containing medium, protection of the enzyme was not complete. (4) Dialysis of cytosol fractions from chicks injected with HgCl2 against 500 vol. of 0.2 M potassium phosphate buffer (pH 7.0) containing 1 mM EDTA and 10 mM dithiothreitol for 4 h at 4 degrees stimulated the fatty acid synthetase activity of the fractions. Dialysis of cytosol fractions from noninjected chicks under the same conditions was without effect on fatty acid synthetase activity. (5) These data support the hypothesis that the inhibitory effect of HgCl2 administered in vivo on hepatic fatty acid synthetase activity in chicks is mediated through the interaction of mercury with the sulfhydryl groups of the enzyme. 相似文献
16.
Rémy Bitoun Philippe Rousselin Michel Caboche 《Molecular & general genetics : MGG》1990,220(2):234-239
Summary Mutant lines of Nicotiana plumbaginifolia resistant to the synthetic auxins 1-naphthaleneacetic acid (NAA) and indole-3-butyric acid (IBA) were isolated as germinating seedlings on selective medium. In each case, resistance was conferred by a single recessive nuclear mutation at one of 3 loci designated iba1, iba2 and iba3. Labelling studies with 14C NAA suggest that resistance was not due to changes in the uptake or metabolism of NAA. Plants homozygous for the iba1 mutation exhibit a syndrome of atypical germination and growth suggestive of a defect in the biosynthesis, metabolism or localization of abscisic acid. Wild-type seeds treated with gibberellin exhibit the same syndrome, including resistance to NAA and IBA. On the basis of these observations, we propose that auxin toxicity in seeds may be mediated by a block in gibberellin biosynthesis.Abbreviations ABA
abscisic acid
- GA
gibberellic acid
- IBA
indole-3-butyric acid
- NAA
1-naphthaleneacetic acid
- p-cell
protoplast-derived cell
- 2,4-D
2,4-dichlorophenoxyacetic acid 相似文献
17.
18.
Robert L. Jarret Paul M. Hasegawa Ray A. Bressan 《In vitro cellular & developmental biology. Plant》1981,17(9):825-830
Summary The formation of adventitious shoots from potato tuber discs explanted onto a modified Murashige and Skoog (MS) medium containingN
6-benzylaminopurine (BAP) (3.0 mg/l), and α-naphthaleneacetic, acid (NAA) (0.01 mg/l), was affected by gibberellic acid (GA).
The presence of GA in the explant medium was required for shoot formation and 3×10−10
M GA appeared optimum. However, microscopic examination of the tissue protuberances on the surface of the tuber discs from
which shoots arose revealed that GA inhibited the formation of shoot meristems. Tuber discs cultured for 6 wk on MS medium
containing BAP and NAA without GA did not initiate adventitious shoots that could be determined visually, but microscopic
examination of the tissue protuberances revealed the presence of numerous shoot meristems. Subsequent transfer of these tuber
discs to medium with GA but without BAP or NAA resulted in the formation of shoots from 100% of the recultrued dises. Thus
it appears that although GA inhibits shoot meristem initiation from potato tuber discs, it is required for shoot development
once meristems are initiated.
This is Journal Paper 8297 of the Purdue University Agricultural Experiment Station. The research was supported by Purdue
University Agricultural Experiment Station Program Improvement Funds. Potato tubers were supplied by Wm. Gehring Farms, Inc.,
Rensselaer, Indiana. 相似文献
19.
The mechanism of gall induction by insects has remained elusive. Previous studies have met with limited success in attempting to induce galls by injection or application of chemical compounds. To determine whether an exogenous source of phytohormones plays a role in gall induction, we injected cytokinin (CK), auxin (IAA), gibberellic acid (GA), and abscisic acid (ABA) in various concentrations, ratios, and combinations into leaf petioles of Capsicum annuum L. cv. California Wonder (Solanaceae). We found that CK + IAA injections lead to gall-like growth in C. annuum. GA enhanced and ABA inhibited gall growth except in the presence of GA. Isopentenyl adenine (IP) was the most effective type of CK at inducing growth. Our work is consistent with the hypothesis that exogenous CK + IAA produced and supplied by insects leads to gall induction. We hypothesize that insects have obtained the capability to induce galls via acquisition of the biosynthetic pathways to produce IAA and trans-zeatin family CKs through microbial symbiosis or lateral gene transfer. 相似文献
20.
利用高效液相色谱法分离和测定棉花组织培养过程中4种内源激素 总被引:8,自引:0,他引:8
目的:优化利用高效液相色谱法测定棉花组织培养过程中吲哚-3-乙酸(IAA)、玉米素(ZT)、赤霉素(GA3)和脱落酸(ABA)等4种植物内源激素的条件,了解棉花胚性愈伤组织发生过程中4种内源激素及激素含量比例的规律性变化,以及添加不同外源激素对内源激素和愈伤组织的影响,为棉花组织培养由经验型变为理论型提供基础。方法:采用高效液相色谱法。结果:棉花胚性愈伤组织发生过程中4种内源激素及激素含量比例呈规律性变化:ZT、ABA、ABA/GA3、ABA/IAA呈现先上升后下降的趋势,GA3呈现先上升后下降再上升的趋势,ZT/IAA呈现明显的上升-下降-上升-下降的趋势;不同激素组合诱导下愈伤组织中内源激素含量及愈伤组织状态也有较大差别。结论:研究结果对指导组织培养过程中激素的调整和配比、制定适宜的培养计划有一定的指导意义;4种不同的内源激素都是愈伤组织生长的重要因子,各自适宜的浓度和恰当的比例调节着外植体的脱分化和再分化,各种激素协同作用促进细胞的生长和分化。 相似文献