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1.
The excitability of firing motoneurones activated by voluntary contraction of the flexor carpi ulnaris or tibialis anterior was tested by single excitatory Ia afferent volleys. In order to estimate the stimulation effects, the peri-stimulus time histograms of single motoneurones were plotted, and the firing indices were calculated. It was shown that the firing-frequency effect was absent within the range of 4-14 imp/s during testing by low-intensity excitatory volley. At higher intensity of afferent volley, the excitability increased at a low firing rate. It is suggested that the characteristics of the interspike-interval excitability trajectories underlie these relations. These findings made it possible to explicate some conflicting literature data which were usually reported without taking the afferent volley intensity effect into account. The mechanisms controlling the firing motoneurone excitability and possible trajectories of interspike-interval membrane potential in human motoneurones are discussed. 相似文献
2.
The activity of single motoneurones of m. flexor carpi ulnaris (FCU) was investigated by recording their motor unit (MU) action potentials during weak and moderate voluntary muscle contractions. The MU firing rate range was 4.5-15 imp/s. The excitability of motoneurones was tested with a number of single stimuli eliciting a monosynaptic H-reflex of low amplitude. Two different indices were defined which relate to motoneuronal excitability: the response index--the ratio of the number of responses of a motoneurone to the total number of stimuli, and the response time--the time after the last background MU discharge at which motoneurone is ready to respond to the excitatory volley. Both the response index and the response time were determined for single motoneurones at different levels of background activity. In the lower range of firing rates, the response index for all motoneurones decreased when increasing the firing rate, but it remained constant in the higher rate range. This kind of response seems to be a typical motoneuronal response to the stimulation with single stimuli. The data on the response time were used to study the excitability of the same single motoneurones to computer simulated repetitive stimulation (stimulation rate 40-100 imp/s). In this case, the excitability of each motoneurone was determined as an increment of its firing rate in response to the stimulation. For the lower firing rate range, the excitability for all motoneurones also decreased when the firing rates increased whereas a variety of slopes was obtained in the higher rate range.(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
3.
P B Matthews 《Journal of Physiology》1999,93(1-2):135-145
This paper reviews two new facets of the behaviour of human motoneurones; these were demonstrated by modelling combined with analysis of long periods of low-frequency tonic motor unit firing (sub-primary range). 1) A novel transformation of the interval histogram has shown that the effective part of the membrane's post-spike voltage trajectory is a segment of an exponential (rather than linear), with most spikes being triggered by synaptic noise before the mean potential reaches threshold. The curvature of the motoneurone's trajectory affects virtually all measures of its behaviour and response to stimulation. The 'trajectory' is measured from threshold, and so includes any changes in threshold during the interspike interval. 2) A novel rhythmic stimulus (amplitude-modulated pulsed vibration) has been used to show that the motoneurone produces appreciable phase-advance during sinusoidal excitation. At low frequencies, the advance increases with rising stimulus frequency but then, slightly below the motoneurones mean firing rate, it suddenly becomes smaller. The gain has a maximum for stimuli at the mean firing rate (the 'carrier'). Such behaviour is functionally important since it affects the motoneurone's response to any rhythmic input, whether generated peripherally by the receptors (as in tremor) or by the CNS (as with cortical oscillations). Low mean firing rates favour tremor, since the high gain and reduced phase advance at the 'carrier' reduce the stability of the stretch reflex. 相似文献
4.
Early inactivation of the slow potassium conductance system (GK), responsible for the spike afterhyperpolarization (AHP) in spinal alpha motoneurones, has been introduced in a motoneurone model whose G
K kinetics give rise to an exponentially decaying AHP conductance. After this modification, the model displays a plateau shaped time-course of the AHP conductance and a faster shortening of the first interval during repetitive firing induced by current steps of increasing intensities. Both features increase the resemblance between the model and the motoneurone behaviour. Comparison with real motoneurones also suggests that G
K inactivation may be more developed in slow than in fast motoneurones. 相似文献
5.
Abdominal motoneurones of the locust Locusta migratoria were investigated in immature, mature and allatectomised females to compare their response characteristics during reproductive development. These motoneurones were chosen because they control muscles which are involved in extreme lengthening during egg-laying behaviour. The study focused on changes in motoneurone firing activity and its possible regulation by juvenile hormone. In isolated nerve-muscle preparations, increased resting motor activity was found in mature (>14 days) but not in immature females (<5 days). Removing the corpora allata, the gland producing juvenile hormone in insects, prevented increased motor activity. Stimulus evoked activation of the motor system led to a characteristic burst of action potentials which lasted for a few seconds. The time-course and amount of activation changed significantly during reproductive development. Mature females displayed longer lasting and higher activity than immature or allatectomised females, but only those segments involved in egg-laying were found to express the altered firing properties. Single cell analysis of motoneurone dendritic morphology or membrane properties revealed no evidence that could be causative for the activity changes seen during reproductive development. The results suggest that altered motoneurone activity serves to adapt females to the neuromuscular requirements of egg-laying behaviour. 相似文献
6.
A model is presented in which the potassium conductance (G
K
) changes responsible for the afterhyperpolarization (AHP) in motoneurones are postulated to follow a kinetics coherent with Hodgkin-Huxley membrane model. Such G
K
kinetics, which operates as a bistable system switched from one state to the other by the action potential, can be expressed by simple analytical expressions if the spike is approximated to a rectangular pulse. Accumulation of G
K
by repetitive activation of the model accurately describes the different features of AHP summation in motoneurones; moreover, this accumulation process enables a model for repetitive firing of motoneurones to display secondary range firing at steady state. 相似文献
7.
F Baldissera 《Archives italiennes de biologie》1984,122(1):43-58
Like receptors, neurones of the mammalian CNS react to both the static and the dynamic features of the input signals. The quantitative aspect of the current-to-frequency transduction during input transients were analyzed in spinal motoneurones and in corticospinal neurones by the technique of injecting intracellularly either ramp of sine-wave currents. It was found that, in both types of neurones, the instantaneous firing frequency is linearly correlated, within definite ranges, both to the intensity and to the velocity of change of the input currents. By recording the isometric mechanogram of the muscle units innervated by the impaled motoneurones, it was disclosed that the motoneurones discharges produced by the ramp currents develop tension changes, whose average velocity is proportional to the ramp slope. For both types of neurones, results are consistent with the hypothesis that the major determinants of the double sensitivity, to the intensity and to the velocity, are the kinetics of the potassium conductance system responsible for the spike afterhyperpolarization. 相似文献
8.
Spinal motoneurones receive thousands of presynaptic excitatory and inhibitory synaptic contacts distributed throughout their dendritic trees. Despite this extensive convergence, there have been very few studies of how synaptic inputs interact in mammalian motoneurones when they are activated concurrently. In the experiments reported here, we measured the effective synaptic currents and the changes in firing rate evoked in cat spinal motoneurones by concurrent repetitive activation of two separate sets of presynaptic neurons. We compared these effects to those predicted by a linear sum of the effects produced by activating each set of presynaptic neurons separately. We generally found that when two inputs were activated concurrently, both the effective synaptic currents and the synaptically-evoked changes in firing rate they produced in motoneurones were generally linear, or slightly less than the linear sum of the effects produced by activating each input alone. The results suggest that the spatial distribution synaptic terminals on the dendritic trees of motoneurones may help isolate synapses from one another, minimizing non-linear interactions. 相似文献
9.
Charles Kaars 《Journal of insect physiology》1979,25(3):209-218
Activity patterns of motoneurones which innervate spiracular muscles in two blaberid cockroaches, Blaberus discoidalis and Gromphadorhina portentosa, have been monitored during two homologous behaviour patterns: respiratory and non-respiratory tracheal ventilation. Based upon the activity of spiracular motoneurones during these two activities, the abdominal spiracles have been divided into three functional groups: vestigial, respiratory and non-respiratory. In Blaberus discoidalis spiracle 3 is vestigial, spiracles 6, 7, 8 and 10 are respiratory, and spiracles 4, 5 and 9 are non-respiratory. In Gromphadorhina portentosa spiracles 3 and 10 are vestigial, spiracle 4 is non-respiratory and spiracles 5–9 are respiratory.Respiratory spiracles in both species are characterized by activity patterns of their motoneurones during respiratory tracheal ventilation: low frequency firing at irregular intervals during the respiratory pause and a higher frequency burst synchronous with the expiratory abdominal compression. Non-respiratory spiracles are characterized by complete inactivity of their opener motoneurones during respiratory tracheal ventilation. These motoneurones are activated by mechanical stimulation in both species, which simultaneously suppresses activity in respiratory opener motoneurones. In Blaberus discoidalis, there are no differences between activity patterns of respiratory and non-respiratory closer motoneurones. In Gromphadorhina portentosa, not only do respiratory and non-respiratory closer motoneurones have different activity patterns, but the activity pattern of respiratory closer motoneurones is different during respiratory and non-respiratory tracheal ventilation. The functional implications of these several spiracular motoneurone activity patterns are discussed. 相似文献
10.
Vatanparast J Janahmadi M Asgari AR 《Comparative biochemistry and physiology. Toxicology & pharmacology : CBP》2006,143(4):464-472
Toxicity of paraoxon has been attributed to inhibition of cholinesterase, but little is known about its direct action on ionic channels. The effects of paraoxon (0.3 microM-0.6 microM) were studied on the firing behaviour of snail neurones. Paraoxon significantly increased the frequency of spontaneously generated action potentials, shortened the afterhyperpolarization (AHP) and decreased the precision of firing. Short periods of high frequency-evoked trains of action potentials led to an accumulation in the depth and duration of post-train AHPs that was evidenced as an increase in time to resumption of autonomous activity. The delay time in autonomous activity initiation was linearly related to the frequency of spikes in the preceding train and the slope of the curve significantly decreased by paraoxon. The paraoxon induced hyperexcitability and its depressant effect on the AHP and the post-train AHP were not blocked by atropine and hexamethonium. Calcium spikes were elicited in a Na+ free Ringer containing voltage dependent potassium channel blockers. Paraoxon significantly decreased the duration of calcium spikes and following AHP and increased the frequency of spikes. These findings suggest that a reduction in calcium influx during action potential may decrease the activation of calcium dependent potassium channels that participate in AHP generation and act as a mechanism of paraoxon induced hyperexcitability. 相似文献
11.
Motoneurones located in lumbar segments (L4-L5) of the rat spinal cord were activated antidromically by stimulation of the sciatic nerve. Records following a single pulse and double stimuli were collected from fast motoneurones and in each case voltage and time parameters of the action potential and afterhyperpolarization (AHP) were compared to respective values calculated for a simulated doublet - obtained by mathematical summation of two individual single potentials. The most prominent differences between experimentally recorded and simulated potentials concerned spike duration and the AHP half-decay times, with significantly lower values obtained for the simulated doublets. Tendencies for a higher AHP amplitude, shorter AHP peak time and higher AHP area were observed in mathematically modeled doublets, though no statistical differences were present in comparison to experimental data. Results indicated a non-linear summation of the AHP after double stimuli running in a short interval. These observations suggest that alterations in the generation of the AHP, mainly based on the sustained activity of K(Na+) channels, influence time-intervals in motoneuronal discharge pattern and this way force development of individual motor units during muscle activity. 相似文献
12.
Gain modulation is a key feature of neural information processing, but underlying mechanisms remain unclear. In single neurons, gain can be measured as the slope of the current-frequency (input-output) relationship over any given range of inputs. While much work has focused on the control of basal firing rates and spike rate adaptation, gain control has been relatively unstudied. Of the limited studies on gain control, some have examined the roles of synaptic noise and passive somatic currents, but the roles of voltage-gated channels present ubiquitously in neurons have been less explored. Here, we systematically examined the relationship between gain and voltage-gated ion channels in a conductance-based, tonically-active, model neuron. Changes in expression (conductance density) of voltage-gated channels increased (Ca2+ channel), reduced (K+ channels), or produced little effect (h-type channel) on gain. We found that the gain-controlling ability of channels increased exponentially with the steepness of their activation within the dynamic voltage window (voltage range associated with firing). For depolarization-activated channels, this produced a greater channel current per action potential at higher firing rates. This allowed these channels to modulate gain by contributing to firing preferentially at states of higher excitation. A finer analysis of the current-voltage relationship during tonic firing identified narrow voltage windows at which the gain-modulating channels exerted their effects. As a proof of concept, we show that h-type channels can be tuned to modulate gain by changing the steepness of their activation within the dynamic voltage window. These results show how the impact of an ion channel on gain can be predicted from the relationship between channel kinetics and the membrane potential during firing. This is potentially relevant to understanding input-output scaling in a wide class of neurons found throughout the brain and other nervous systems. 相似文献
13.
B Gustafsson 《Archives italiennes de biologie》1984,122(1):17-30
In mammalian central neurones, the soma-dendritic spike is generally followed by afterpotentials, a brief depolarizing potential (delayed depolarization) and a more longlasting afterhyperpolarization (AHP). These afterpotentials, and in particular the AHP, have long been considered as important factors in the control of excitation-to-frequency transduction. Analysis of the afterpotential properties has been performed on various types of central neurones; spinal alpha-motoneurones, dorsal spinocerebellar tract cells, rubrospinal neurones and hippocampal CA1 pyramidal cells. These investigations have shown the afterpotentials to differ considerably in their characteristics among these types of neurones. Studies of the firing behaviour of the neurones have also shown great variations in their firing properties, the observed differences being well in accord with those expected on the basis of their different afterpotential characteristics. The results suggest that the afterpotentials play a major role in the control of excitation-to-frequency transduction in several types of central neurones. 相似文献
14.
The development of a 'two-site' immunoradiometric assay for human prolactin (hPrl) is described. The assay is based on the addition of radio-iodinated sheep anti-hPrl immunoglobulin G (IgG) and rabbit anti-hPrl serum to standards and unknowns followed by 3 h incubation. The use of solid phase reagents was avoided in order to minimize non-specific effects and the time required for reactants to reach equilibrium. Instead, the separation of hPrl-bound and free labelled antibody is achieved by the addition of sheep anti-(rabbit IgG) serum which precipitates bound labelled antibody by complex formation with rabbit anti-hPrl antibodies which are also hPrl-bound. Varying the order of addition of specific antibodies had a pronounced effect on the 'operating range' and sensitivity of resultant assays. This was attributed to competition between labelled and unlabelled antibodies for binding sites on the hPrl molecule. The immunoradiometric assay employing 'simultaneous addition' of specific antibodies was compared to a 'simultaneous addition' hPrl radioimmunoassay developed using the same sheep antiserum as that used to prepare the radioiodinated sheep anti-hPrl IgG. This immunoradiometric assay is characterized by rapid equilibration of reactants, a wide 'operating range' (the precision of dose estimates was less than 10% over the range 8-10000 mU/l), and high sensitivity (2.6 mU/l, 13 pg). In contrast, the hPrl radioimmunoassay required an incubation of 18 h, demonstrated a much reduced 'operating range' (the precision of dose estimates was less than 10% only over the range 25-1500 mU/l) and reduced sensitivity (9.8 mU/l, 49 pg). 相似文献
15.
Friedemann Awiszus 《Biological cybernetics》1994,70(6):579-583
Afferent activity in a receptor afferent fiber with several encoding sites is generally believed to represent the activity of the fastest pacemaker that resets all more slowly encoding sites. Alternatively, some impulse mixing as well as some nonlinear summation of receptor current to a single encoder have been considered. In this article the repetitive firing activity of a Hodgkin-Huxley axon consisting of two branches that join into a single stem axon was investigated. The model axon was stimulated by constant-current injection into either the right or the left or both branches. It was found that the model axon generated an (infinite) train of action potentials if the input current was large enough. The discharge frequency found was constant, and on combined stimulation of both branches with different current, the site of impulse initiation was always in the branch receiving the higher input current, excluding a simple impulse mixing. On the other hand, the combined stimulation of both branches evoked repetitive firing with a higher frequency than expected by the pacemaker-resetting hypothesis. Moreover, a stimulus that is subthreshold for repetitive firing if injected into one branch yields repetitive firing when it is injected into both branches, a behavior inconsistent with impulse mixing and pacemaker resetting. On the other hand, current injection into one branch allowed repetitive activity only within a rather limited range of firing frequencies. Using distributed current injection into both branches, however, allowed many more different firing frequencies. Such behavior is inconsistent with both pacemaker resetting and (nonlinear) input current summation. Consequently, the repetitive firing behavior of a branched Hodgkin-Huxley axon with multiple encoding sites appears to be more complex than postulated in the simple hypotheses. 相似文献
16.
The present study was aimed at determining if inputs from the locus coeruleus (LC) have any effect on repetitive firing of ventral horn motoneurons in cats. In hindlimb flexor and extensor motoneurons stimulated intrasomatically with current below the threshold for repetitive discharges, added LC-evoked excitatory post-synaptic potentials (EPSPs) were consistently found to produce repetitive firing, suggesting a lowering in the repetitive firing threshold attributable to excitatory LC inputs. With those extensor motoneurons showing episodic, self-sustained firing, LC-EPSPs introduced during the quiescent period were capable of starting a continuous discharge with rhythmic frequencies higher than the spontaneous activity. In some of these cells, intracellularly applied hyperpolarizing current was able to stop the spontaneous discharges. Subsequently, LC stimuli were found to reinitiate repetitive discharges, thus counteracting the ongoing suppression of the motoneurons. Quantitative analysis of the single-spike afterhyperpolarization (AHP) indicated a consistent reduction in its amplitude and time course (duration, time-to-peak, half-decay time) for flexor and extensor motoneurons in response to LC conditioning stimuli. Present results suggest an excitatory LC action on the repetitive discharges of cat motoneurons accompanied by a concurrent decrease in the amplitude and time course of the single-spike AHPs. 相似文献
17.
M V Chistiakova P M Balaban 《Zhurnal vysshe? nervno? deiatelnosti imeni I P Pavlova》1988,38(6):1060-1067
Bath application of 10(-5) mol/l of serotonin (5-HT) elicited a 50% increase of summary EPSPs recorded in command neurones for avoidance behaviour. No significant changes of rest potential and input resistance were seen in these cells. 5-HT evoked an increase of spontaneous level of firing in motoneurones involved in the same reflex, as well as an increase in the number of spikes which paralleled increase of EPSPs to the same stimulus in command neurones. In sensory cells, presynaptic to the command neurones, application of 5-HT evoked a significant increase of excitability and of input resistance. Monosynaptic EPSPs recorded in the command neurones showed a 40% increase after serotonin application. It is concluded that the major locus of plastic changes evoked by 5-HT application in the neuronal chain underlying avoidance reflex is the synaptic contact between sensory and command neurones. 相似文献
18.
Afferent innervation patterns in the vestibular periphery are complex, and vestibular afferents show a large variation in their regularity of firing. Calyx fibers terminate on type I vestibular hair cells and have firing characteristics distinct from the bouton fibers that innervate type II hair cells. Whole-cell patch clamp was used to investigate ionic currents that could influence firing patterns in calyx terminals. Underlying K(Ca) conductances have been described in vestibular ganglion cells, but their presence in afferent terminals has not been investigated previously. Apamin, a selective blocker of SK-type calcium-activated K(+) channels, was tested on calyx afferent terminals isolated from gerbil semicircular canals during postnatal days 1-50. Lowering extracellular calcium or application of apamin (20-500?nM) reduced slowly activating outward currents in voltage clamp. Apamin also reduced the action potential afterhyperpolarization (AHP) in whole-cell current clamp, but only after the first two postnatal weeks. K(+) channel expression increased during the first postnatal month, and SK channels were found to contribute to the AHP, which may in turn influence discharge regularity in calyx vestibular afferents. 相似文献
19.
Palmer AR 《Current opinion in neurobiology》2004,14(4):457-460
Interaural time differences (ITDs) are the dominant cues for human localisation of low-frequency sounds. Although a mechanism for ITD processing proposed in 1948 seems applicable to birds, and is consistent with many aspects of the responses found in mammals, recent data suggest that key tenets of the model might need to be reconsidered. The model requires, at every frequency, a distribution of cells with firing rate peaks across all ITD values within the animal's physiological range. The ITD tuning relies on internal delays in the form of a neural delay line. The evidence for such a delay line structure in mammals is not as convincing as it is in birds and, in some small animals the full range of physiological ITDs are not fully represented by peak firing of neurones at every frequency channel. Alternative means of achieving internal delays such as inhibitory inputs or the delays associated with cochlear filtering are being considered. 相似文献
20.
We examined the effects of 50-Hz magnetic fields in the range of flux densities relevant to our current environmental exposures
on action potential (AP), after-hyperpolarization potential (AHP) and neuronal excitability in neurons of land snails, Helix aspersa. It was shown that when the neurons were exposed to magnetic field at the various flux densities, marked changes in neuronal
excitability, AP firing frequency and AHP amplitude were seen. These effects seemed to be related to the intensity, type (single
and continuous or repeated and cumulative) and length of exposure (18 or 20 min). The extremely low-frequency (ELF) magnetic
field exposures affect the excitability of F1 neuronal cells in a nonmonotonic manner, disrupting their normal characteristic
and synchronized firing patterns by interfering with the cell membrane electrophysiological properties. Our results could
explain one of the mechanisms and sites of action of ELF magnetic fields. A possible explanation of the inhibitory effects
of magnetic fields could be a decrease in Ca2+ influx through inhibition of voltage-gated Ca2+ channels. The detailed mechanism of effect, however, needs to be further studied under voltage-clamp conditions. 相似文献