Food plants of the Luo of Siaya district,Kenya

Plants used for food by the Luo-speaking people of Siaya District, Kenya, were surveyed as part of a comprehensive ethnobotanical study. Fifty-two crops were observed under cultivation in the district; 69 species are gathered from the wild. Wild fruits, underground portions, leaves, and fungal fruiting bodies are probably important in Siaya as dietary supplements. These non-cultivated resources, particularly important in the driest areas of the district, warrant evaluation for their role as a buffer against periods of food shortage.     

Patriliny Among the Gusii and the Luo of Kenya

The Nilotic Luo of Kenya, in contrast to the Nuer, experienced considerable land scarcity. They responded with increasing hostility as much to their affines as to the colonial power which had enclosed land. Exclusive local patrilineages emerged, expressing themselves in collective feuding, litigation, and ritual toward immediate ancestors. A similar process seems to have occurred among the sociologically similar neighboring Bantu-speaking Gusii .     

Herbal remedies improve the strength of repairing ligament in a rat model.

Herbal remedies have been reported to be effective in controlling inflammation for acute soft tissue injuries. There exist, however, no reports of their effects on collagen production and remodeling; thus mechanical strength studies of the tissues have not been reported. This study tested the effects of a herbal remedy on the strength of healing medial collateral ligaments (MCL) in rats. Sixteen rats receiving surgical transection to their right MCLs and eight receiving sham operation were tested. Eight of the MCL-injured animals were treated with an adhesive herbal plaster application to their right knees, while the other eight in the MCL injured group and the sham group were treated with plain adhesive plaster to their right knees. The MCLs were harvested and tested at either 3 or 6 weeks post-operation. The ultimate tensile strength (UTS) and stiffness normalized to the uninjured side of each animal of the herb and sham groups were significantly larger than those of the control at both 3 and 6 weeks (p = 0.001). No significant difference was found in stiffness between the herb and sham groups (p > 0.05). We concluded that the herbal remedy improves the UTS and stiffness of repairing MCLs at 3 and 6 weeks after injury.     

Participatory investigations of bovine trypanosomiasis in Tana River District, Kenya

Participatory research on bovine trypanosomiasis was conducted with Orma pastoralists in Tana River District, Kenya. The use of participatory methods to understand local perceptions of disease signs, disease causes, disease incidence by cattle age group, seasonal patterns of disease and preferences for indigenous and modern control methods are described. Results indicated that local characterization of diseases called gandi and buku by Orma pastoralists was similar to modern veterinary knowledge on chronic trypanosomiasis and haemorrhagic trypanosomiasis (due to Trypanosoma vivax), respectively. The mean incidence of gandi varied from 10.2% in calves to 28.6% in adult cattle. The mean incidence of buku varied from 3.1% in calves to 9.6% in adults. Pearson correlation coefficients for disease incidence by age group were 0.498 (P < 0.01) and 0.396 (P < 0.05) for gandi and buku, respectively. Informants observed cases of trypanosomiasis in 24.1% of cattle (all age groups); these cases accounted for 41.8% of all sick cattle during the preceding 12-month period. Eight indigenous and three modern trypanosomiasis control methods were identified. Results indicated that an integrated approach to trypanosomiasis control based on private, individual action was well established in the assessment area. When presented with four different trypanosomiasis control methods, community representatives selected 'better use of trypanocides' as the most preferred intervention and 'community-based tsetse control' as the least preferred intervention. This finding prompted researchers to modify the original project activities. Constraints facing the sustainability of community-based tsetse control are discussed.     

Effects of drought on child health in Marsabit District,Northern Kenya

This study uses five years of panel data (2009–2013) for Northern Kenya’s Marsabit district to analyze the levels and extent of malnutrition among children aged five and under in that area. We measure drought based on the standardized normalized difference vegetation index (NDVI) and assess its effect on child health using mid-upper arm circumference (MUAC). The results show that approximately 20 percent of the children in the study area are malnourished and a one standard deviation increase in NDVI z-score decreases the probability of child malnourishment by 12–16 percent. These findings suggest that remote sensing data can be usefully applied to develop and evaluate new interventions to reduce drought effects on child malnutrition, including better coping strategies and improved targeting of food aid.     

Functional tissue engineering of tendon: Establishing biological success criteria for improving tendon repair

Improving tendon repair using Functional Tissue Engineering (FTE) principles has been the focus of our laboratory over the last decade. Although our primary goals were initially focused only on mechanical outcomes, we are now carefully assessing the biological properties of our tissue-engineered tendon repairs so as to link biological influences with mechanics. However, given the complexities of tendon development and healing, it remains challenging to determine which aspects of tendon biology are the most important to focus on in the context of tissue engineering. To address this problem, we have formalized a strategy to identify, prioritize, and evaluate potential biological success criteria for tendon repair. We have defined numerous biological properties of normal tendon relative to cellular phenotype, extracellular matrix and tissue ultra-structure that we would like to reproduce in our tissue-engineered repairs and prioritized these biological criteria by examining their relative importance during both normal development and natural tendon healing. Here, we propose three specific biological criteria which we believe are essential for normal tendon function: (1) scleraxis-expressing cells; (2) well-organized and axially-aligned collagen fibrils having bimodal diameter distribution; and (3) a specialized tendon-to-bone insertion site. Moving forward, these biological success criteria will be used in conjunction with our already established mechanical success criteria to evaluate the effectiveness of our tissue-engineered tendon repairs.     

Food of three upland game birds in Selengei Area, Kajiado District, Kenya

Foods taken by yellow-necked spurfowl, crested francolin and helmeted guinea-fowl were investigated over a 22-month period. Bulblets of sedge (Cyperus sp.) were the major plant item in the diet of all three species. Other foods were seeds and fruits of forbs, florets and fruits of grasses, leaves of forbs and grasses and insects. Insect use increased following the rains, as did the use of florets of the grasses. Except for Cyperus sp. there was little overlap of food items taken by the crested francolin and the guinea-fowl, but both were in competition with the yellow-necked spurfowl for some species of plants. This was probably due to size of food items, that is, there was a direct relationship between size of bird and size of foods taken. Forag ing habits also influence food intake.     

Herbal remedies traditionally used against malaria in Ghana: bioassay-guided fractionation of Microglossa pyrifolia (Asteraceae)

Different extracts from 11 West African plants traditionally used against malaria in Ghana were tested against both the chloroquine-sensitive strain PoW and the chloroquine-resistant clone Dd2 of Plasmodium falciparum. Due to the promising in vitro activity of the lipophilic extract [IC50: 10.5 microg/ml (PoW); 13.1 microg/ml (Dd2)], Microglossa pyrifolia (Lam.) Kuntze (Asteraceae) was chosen for further phytochemical investigation. From active fractions 13 compounds were isolated; their structures were established on the basis of spectroscopic methods. 1-Acetyl-6E-geranylgeraniol-19-oic acid and sinapyl diangelate represent new natural compounds. The two diterpenes E-phytol [IC50: 8.5 microM (PoW); 11.5 microM (Dd2)], and 6E-geranylgeraniol-19-oic acid [IC50: 12.9 microM (PoW); 15.6 microM (Dd2)] proved to be the most active constituents in our test system.     

Determinants of teenage pregnancies: The case of Busia District in Kenya

Sub-Saharan Africa has one of the highest levels of teenage pregnancies in the world. In spite of that, there is paucity of empirical research on causes of teenage pregnancies in African countries. This paper investigates the determinants of teenage pregnancies based on a case study of Busia District in Kenya. The data are from a household survey conducted in 1998/1999. Empirical results indicate that girls' education level has significant influence on the probability of teenage birth, with non-schooling adolescents and those with primary school level education being more vulnerable. Among the variables used as proxies for access to sex education, availability of church forums that educate adolescents about sex and family life issues reduce probability of teenage pregnancy. Age is positively related to teenage pregnancies, with older adolescents being more predisposed to pregnancies. Though use of contraceptives is found to have a positive effect, only a small proportion of adolescents were using modern contraceptives and, supply side factors such as quality and availability were not accounted for. Other key factors as outlined by the adolescents themselves include peer pressure and social environment-related factors like inappropriate forms of recreation, which act as rendezvous for pre-marital sex, as well as lack of parental guidance and counselling. Overall, lack of access to education opportunities, sex education and information regarding contraceptives, as well the widespread poverty predispose girls to teenage pregnancies. The problem of teenage pregnancies should be viewed within the broader socio-economic and socio-cultural environment in which the adolescents operate. For instance, lack of parental guidance on issues of sexuality and sex education was reinforced by cultural taboos that inhibit such discussions. Adolescents should be equipped with the relevant knowledge to enable them make informed choices regarding sexual relationships. This should be complemented with broader programmes aimed at promoting girl education and poverty alleviation.     

The prevalence and risk factors for pneumococcal colonization of the nasopharynx among children in Kilifi District, Kenya

Background

Pneumococcal conjugate vaccines (PCV) reduce nasopharyngeal carriage of vaccine-serotype pneumococci but increase in the carriage of non-vaccine serotypes. We studied the epidemiology of carriage among children 3–59 months old before vaccine introduction in Kilifi, Kenya.

Methods

In a rolling cross-sectional study from October 2006 to December 2008 we approached 3570 healthy children selected at random from the population register of the Kilifi Health and Demographic Surveillance System and 134 HIV-infected children registered at a specialist clinic. A single nasopharyngeal swab was transported in STGG and cultured on gentamicin blood agar. A single colony of pneumococcus was serotyped by Quellung reaction.

Results

Families of 2840 children in the population-based sample and 99 in the HIV-infected sample consented to participate; carriage prevalence was 65.8% (95% CI, 64.0–67.5%) and 76% (95% CI, 66–84%) in the two samples, respectively. Carriage prevalence declined progressively with age from 79% at 6–11 months to 51% at 54–59 months (p<0.0005). Carriage was positively associated with coryza (Odds ratio 2.63, 95%CI 2.12–3.25) and cough (1.55, 95%CI 1.26–1.91) and negatively associated with recent antibiotic use (0.53 95%CI 0.34–0.81). 53 different serotypes were identified and 42% of isolates were of serotypes contained in the 10-valent PCV. Common serotypes declined in prevalence with age while less common serotypes did not.

Conclusion

Carriage prevalence in children was high, serotypes were diverse, and the majority of strains were of serotypes not represented in the 10-valent PCV. Vaccine introduction in Kenya will provide a natural test of virulence for the many circulating non-vaccine serotypes.     

Assessing extinction risk in the absence of species-level data: quantitative criteria for terrestrial ecosystems

The conservation of individual plant and animal species has been advanced greatly by the World Conservation Union’s (IUCN) development of objective, repeatable, and transparent criteria for assessing extinction risk, which explicitly separate the process of risk assessment from priority-setting. Here we present an analogous procedure for assessing the extinction risk of terrestrial ecosystems, which may complement traditional species-specific risk assessments, or may provide an alternative when only landscape-level data are available. We developed four quantitative risk criteria, derived primarily from remotely sensed spatial data, information on one of which must be available to permit classification. Using a naming system analogous to the present IUCN species-specific system, our four criteria were: (A) reduction of land cover and continuing threat, (B) rapid rate of land cover change, (C) increased fragmentation, and (D) highly restricted geographical distribution. We applied these criteria to five ecosystems covering a range of spatial and temporal scales, regions of the world, and ecosystem types, and found that Indonesian Borneo’s lowland tropical forests and the Brazilian Atlantic rainforest were Critically Endangered, while South Africa’s grasslands and Brazil’s Mato Grosso were Vulnerable. Furthermore, at a finer grain of analysis, one region of Venezuela’s coastal dry forests (Margarita Island) qualified as Vulnerable, while another (the Guasare River watershed) was Critically Endangered. In northern Venezuela, deciduous forests were classified as Endangered, semi-deciduous forests Vulnerable, and evergreen forests of Least Concern. We conclude that adoption of such a standardized system will facilitate globally comparable, repeatable geographic analyses that clearly separate risk assessment (a fundamentally scientific process), from the definition of conservation priorities, which should take into account additional factors, such as ecological distinctiveness, costs, logistics, likelihood of success, and societal preferences. Jon Paul Rodríguez and Jennifer K. Balch are contributed equally to this work     

A new genus of large primate from the Late Oligocene of Lothidok, Turkana District, Kenya

The species Proconsul (Xenopithecus) hamiltoni was based on two weathered molars in a maxillary fragment from the Erageleit beds, Lothidok, Turkana District, Kenya. New fossils from Lothidok show a unique suite of features indicating generic distinction of this taxon from other known large early catarrhines. A new generic diagnosis is given. The new genus, although still poorly known, may be the first Oligocene hominoid from East Africa. It retains many primitive features, but the few derived ones hint that it may be related to the Afropithecus group.     

Challenges of conservation of dryland shallow waters,Ewaso Narok swamp,Laikipia District,Kenya

Ewaso Narok Swamp, formed along the Eng'are Narok river, is located in the semi-arid part of the Laikipia District, Kenya. The area, of bushy grassland, is characterised by low rainfall (less than 750 mm annually) and episodic rivers. Before the 1970s, the dominant land use was large scale ranching and nomadic pastoralism. Since 1970, this has slowly been transformed into high density small-scale farming. There has been a strong trend towards settlement along riverine and wetland areas due to their suitability for farming and easy availability of water for cultivation. Ewaso Narok swamp has a rich species diversity of over 170 bird species, resident and migrant, over 100 plant species and it also provides an important dryland refuge for both domestic and wild animals. The swamp also provides socio-economic products such as plant matter for building. The result of its land use transformation has been ecosystem alteration, habitat modification and destruction both for wetland and rangeland species. This change has also been accompanied by escalating human–wildlife conflict. However, although this process is self-destructive, the lack of economic returns from wildlife to some extent justify the land use transformation since the communities settling here have to satisfy the basic requirements of food and shelter. This poses the challenge of the developing appropriate ways to conserve the dryland wetlands whilst attaining maximum returns for the local community.     

Herbal remedies for the management of seed-borne fungal pathogens by an edible plant Decalepis hamiltonii (Wight & Arn)

Abstract

Antifungal activity-guided assay of solvent extracts of Decalepis hamiltonii (Wight & Arn) (Asclepiadaceae) against important phytopathogenic fungi, known to cause diseases in sorghum, maize and paddy proved to be highly significant. Among the five solvent extracts tested, Petroleum ether extract showed highly significant antifungal activity. Phytochemical analysis revealed that the antifungal active principle is a phenolic compound. TLC separation of the phenolic fraction using chloroform as an eluting solvent revealed the presence of seven bands but the antifungal activity was observed only in band five with R_f value 0.77. The antifungal active compound is identified as 2-hydroxy-4-methoxybenzaldehyde based on Nuclear Magnetic Resonance (NMR) and mass spectral analysis. The Minimal inhibitory concentration (MIC) varied between 200 μg ml^?1 and 700 μg ml^?1 depending on the fungal species. Seed treatment of the active principle significantly increased seed germination and seed vigour with a corresponding decrease in seed mycoflora. The antifungal active compound was effective against all the 24 fungal species tested suggesting broad-spectrum antifungal activity. Comparative evaluation of the active principle with the synthetic fungicides revealed that the antifungal activity of the active principle obtained from the plant is better than that of synthetic fungicide. This plant being an edible one can be exploited in the management of seed-borne pathogenic fungi and the prevention of biodeterioration of grains and mycotoxin elaboration during storage.     

Establishing a procedure for dimethyl sulfoxide removal from cardiovascular allografts: a quantitative study

The success of allotransplants is critically dependent on both tissue viability and efficient removal of potentially toxic cryopreservants. In this study we analysed the dimethyl sulphoxide (Me2SO) content of cardiovascular tissue samples stored in tissue banks and optimized a washing protocol to be used before surgical implant. We compared the Me2SO content of heart valves, arteries and veins and quantitatively determined by HPLC the washing kinetics of each group of tissue samples under strictly controlled conditions using an industrial washing medium (BASE). Our results showed that heart valves and arteries have significantly slower Me2SO release kinetics than veins. Approximately 20 % of the initial content of cryopreservant could still be detected in the valves and arterial tissue after 15 min of continuous washing. Conversely, veins were almost completely cleared of the cryoprotectant under the same conditions. We propose a washing protocol consisting of two sequential washing with BASE for a total of 25 min for valves and arteries and 15 min for veins. In our hands, this protocol reliably ensures the removal of more than 95 % of the initial Me2SO content.     

Creodonts and carnivores from the Middle Miocene Muruyur Formation at Kipsaraman and Cheparawa,Baringo District,Kenya

The Middle Miocene Muruyur Formation (ca 14.5 Ma), Tugen Hills, Kenya, has yielded a huge creodont and a variety of carnivores ranging in size from mongoose-sized viverrids and herpestids to lion-sized amphicyonids. The fauna partly fills what used to be a major gap in our knowledge of Neogene African carnivores, spanning the period between the better known Early Miocene assemblages of western Kenya and eastern Uganda, and the Late Miocene and Plio–Pleistocene faunas of East Africa. Present in the deposits are Megistotherium, two species of Hecubides, one species of Agnotherium, Herpestes, Vishnuictis, and one or two undetermined felids.