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1.
Pheromones are innate products, exploited for direct population manipulation in the trapping of precise pest density. They demonstrate feasibility and efficiency against Helicopverpa armigera at five different cotton-based agro-ecological sites: river site, vegetable farm, orchard, clean cultivation site, and forest. The data were recorded at 15–20 day intervals from 8 September 2004 to 1 December 2006. The Number of moths caught during the study period demonstrated that the vegetable farm showed the maximum population of moths per trap (0.54), followed by the orchard (0.29), forest (0.19), river (0.15), and clean cultivation site (0.12). During 2004, rainfall (in the orchard and clean cultivation site) and relative humidity (in the clean cultivation site) showed positive and significant correlation with the number of moths caught. During 2005, temperatures played a significant and positive role in population fluctuation of the pest almost at all the selected sites; the minimum temperature was recorded at the vegetable farm and the average temperature was found at the forest site. Rainfall also showed a significant and positive correlation with trapped moths in the vegetable farm, orchard, and clean cultivation site. During 2006, relative humidity showed a significant and negative correlation with the trapped population at all the sites except the river site. Rainfall showed a negative and significant correlation with the pest at the forest site. Relative humidity was the most important aspect at 8.93% causing variation in the trapped population followed by maximum temperature (5.7%), rainfall (1.63%), average temperature (0.74%), and minimum temperature (0.56%). 相似文献
2.
本从气候因子、天敌、耕作栽培措施、诱集技术、生物制剂和转Bt基因棉等几个方面对安徽棉铃虫种群的生态控制技术研究进行了总结,表明各种生物控制措施对控制棉铃虫的种群是有效的;种群系统分析将是研究棉铃虫生态控制研究的主要方法,建立科学合理的生态控制系统是棉铃虫控制研究的重点。 相似文献
3.
Jian Huang 《Ecology and evolution》2021,11(24):18357
The effects of climate change on pest phenology and population size are highly variable. Understanding the impacts of localized climate change on pest distribution and phenology is helpful for improving integrated pest management strategies. Here, the population dynamics of cotton bollworms (Helicoverpa armigera) from Maigaiti County, south Xinjiang, and Shawan County, north Xinjiang, China, were analyzed using a 29‐year dataset at lower latitudes and a 23‐year dataset at higher latitudes to determine the effects of climate change on the population dynamics of H. armigera. The results showed that all generations of H. armigera at both sites showed increasing trends in population size with climate warming. Abrupt changes in phenology and population number occurred after abrupt temperature changes. Climate change had a greater effect on the phenology of H. armigera at higher latitudes than at lower latitudes and led to a greater increase in population size at lower latitudes than at higher latitudes; the temperature increase at higher latitudes will cause a greater increase in the adult moth population size in the future compared to that at lower latitudes; and abrupt changes in the phenology, temperature increase, and population size at lower latitudes occurred earlier than those at higher latitudes. Thus, it is necessary to develop sustainable management strategies for Helicoverpa armigera at an early stage. 相似文献
4.
Helicoverpa armigera (Hübner) is one of the most prominent polyphagous species of the Heliothinae pest complex that inflicts severe damage to a wide range of crops in India. Knowledge regarding the population structure of the pest species, whether morphological or genetic, is considered as an essential tool in making effective management decisions. Thus here, we performed the phenotypic characterization of H. armigera populations collected from varied geographic locations across India. Studied populations differed significantly for several external morphometric traits studied at larval, pupal and adult stages. Significant differences were also observed with respect to the intensity of black pigmentation on larval body as well as adult eye and forewing colour patterns. Besides external phenotypic traits, the length of genital organs like aedeagus and valva in males, and bursa copulatrix and bursa seminalis in females also differed significantly amongst populations. The dendrogram based on selected traits showed clear cut differentiation of studied populations into two major groups, one including all the South Zone populations and the other having populations from North and Central Zones. Differences based on phenotyping in the present study indicate the possibility of the existence of different subspecies within the Indian populations of H. armigera. 相似文献
5.
Hanumanth G. Goudru Sathish Kumar Senigala K. Jayalakshmi Chandish R. Ballal Hari C. Sharma Kuruba Sreeramulu 《Entomological Research》2013,43(1):55-62
Phenoloxidases are oxidative enzymes, which play an important role in both cell mediated and humoral immunity. Purification and biochemical characterization of prophenoloxidase from cotton bollworm, Helicoverpa armigera (Hübner) were carried out to study its biochemical properties. Prophenoloxidase consists of a single polypeptide chain with a relative molecular weight of 85 kDa as determined by SDS–PAGE, MALDI–TOF MS and LC–ESI MS. After the final step, the enzyme showed 71.7 fold of purification with a recovery of 49.2%. Purified prophenoloxidase showed high specific activity and homology with phenoloxidase subunit‐1 of Bombyx mori and the conserved regions of copper binding (B) site of phenoloxidase. Purified prophenoloxidase has pH optima of 6.8 and has high catalytic efficiency towards the dopamine as a substrate in comparison to catechol and L‐Dopa. The PO activity was strongly inhibited by phenylthiourea, thiourea, dithiothreitol and kojic acid. 相似文献
6.
飞行过程中棉铃虫对温度的主动选择 总被引:1,自引:0,他引:1
为了探讨温度对迁飞性昆虫空中群体聚集成层等行为的作用机制,在室内利用自行设计的连续温度梯度发生装置对棉铃虫Helicoverpa armigera在飞行过程中的温度选择行为进行了研究.结果表明:在存在明显温差的连续温度梯度中,所有棉铃虫飞行个体均对温度具有显著的选择行为.棉铃虫试虫群体表现出对空间最优飞行温度共同的主动选择,选择的温度范围是20~22℃之间;在这一温度范围内棉铃虫的振翅频率最高,持续振翅时间最长.对不同温度梯度条件下各温度区间内试虫的飞行时间进行定量比较发现,在16~22℃温度梯度场中的棉铃虫群体对最适温度的选择比在19~30℃的温度梯度场中的群体更显著,表明在温度较低的迁飞季节中温度对迁飞棉铃虫空中虫群聚集成层的影响要比在高温季节更明显.持续飞行时间对棉铃虫振翅频率的影响明显,表明昆虫迁飞高度与昆虫自身能量的消耗存在联系. 相似文献
7.
不同龄期棉铃虫用氰戊菊酯汰选对其抗性发展的影响 总被引:2,自引:0,他引:2
利用中抗(15.06倍)品系,室内用氰戊菊酯对棉铃虫Helicoverpa armigera初孵至4龄幼虫分别进行连续汰选,选育9代后,以3龄幼虫汰选的抗性发展最快(31.5倍), 其次是4龄、2龄幼虫汰选的品系(分别增加25.2倍和14.5倍),用初孵幼虫汰选的抗性发展最慢(10.2倍)。抗性现实遗传力的测定表明,3龄幼虫汰选的抗性现实遗传力(0.4419)显著大于初孵幼虫的(0.2346)。代谢酶抑制剂的增效实验发现,磷酸三苯酯(TPP)对各品系棉铃虫均无明显增效作用。而增效醚(PBO)对高龄幼虫汰选的品系的增效作用比低龄幼虫汰选的品系增效作用强。测定初孵和3龄幼虫汰选品系试虫的击倒抗性发现,初孵幼虫汰选品系的抗性增加倍数(10.2)与击倒抗性增加的倍数(10.5)相似,而3龄幼虫汰选的抗性增加倍数(31.5)显著高于击倒抗性增加的倍数(19.9)。认为初孵幼虫期多功能氧化酶(MFO)表达不完全,用药主要是筛选击倒抗性,而高龄幼虫期用药则会同时筛选击倒抗性和MFO参与的代谢抗性。因而初孵幼虫期用药抗性发展缓慢。生产上不仅可以提高药剂的防效,同时可以延缓抗性的发展。 相似文献
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9.
A study was carried out at the University of Arid Agriculture, Rawalpindi, Pakistan to integrate different control measures against Helicoverpa armigera (Hübner) on chickpea, to minimize the use of hazardous insecticides and develop an eco-friendly management program. Cultural (weeding), mechanical (hand picking), biological (release of Trichogramma chilonis ), microbial (spraying of Bacillus thruingiensis ) and chemical (Steward) control practices were applied three times at 10 day intervals individually and also in various combinations. The application of Steward proved most effective when applied alone, with 0.41 larvae/plant, pod infestation of 9.31% and the highest grain yield (1203.66 g/plot); however, the integration of weeding, hand picking and Steward proved to be the most effective in reducing the larval population (0.12 larvae/plant) with minimum pod infestation (5.45%) on variety CM-2000, which resulted in the maximum grain yield (1260.33 g/plot). The cost–benefit ratio in the treatment where Steward was applied alone was 1:2.20, and it was 1:3.53 where weeding and hand picking practices were integrated. 相似文献
10.
Nagalakshmi VK Applebaum SW Azrielli A Rafaeli A 《Archives of insect biochemistry and physiology》2007,64(3):142-155
Insect males produce accessory gland (MAG) factors that are transferred in the seminal fluid to females during copulation, and elicit changes in the mated female's behavior and physiology. Our previous studies showed that the injection of synthetic Drosophila melanogaster sex-peptide (DrmSP) into virgin females of the moth Helicoverpa armigera causes a significant inhibition of pheromone production. In this and other moth species, pheromone production, correlated with female receptivity, is under neuroendocrine control due to the circadian release of the neuropeptide PBAN. In this study, we show that PBAN, present in the hemolymph during the scotophase in females, is drastically reduced after mating. We also identify 4 DrmSP-like HPLC peaks (Peaks A, S1, S2, and B) in MAGs, with increasing levels of DrmSP immunoreactivity during the scotophase, when compared to their levels observed during the photophase. In H. armigera MAGs, a significant reduction in the pheromonostatic peak (Peak B) was already evident after 15 min of copulation, and depletion of an additional peak (Peak S2) was evident after complete mating. Peak A is also detected in female brains, increasing significantly 1 h after mating, at which time inhibition of pheromone biosynthesis also occurs. However, changes corresponding to the other MAG peaks were not detected in mated female tissues. 相似文献
11.
两种性信息素诱捕器对棉铃虫雄蛾的诱捕效果比较 总被引:5,自引:0,他引:5
1995年在山东和安徽棉区4个地点比较研究了性信息素笼罩诱捕器和水盆诱捕器对棉铃虫雄蛾的诱捕效果。试验结果表明,4地点笼罩诱蛾量分别是水盆诱蛾量的2.8、3.5、2.5和2.0倍,平均2.7倍。两种诱捕器诱蛾量倍数的变异系数分别为22.9%、31.5%、50.7%和51.2%。可见笼罩诱捕器比水盆诱捕器对棉铃虫雄虫具有更高的诱捕效率。考虑到笼罩诱捕器高效、稳定及简便,建议在我国尽快用其取代水盆进行棉铃虫成虫的标准化监测。 相似文献
12.
Abstract: In Helicoverpa armigera , withdrawal of selection pressure resulted in a two- to fourfold increase in susceptibility to synthetic pyrethroids and continuous selection enhanced the resistance level by four- to fivefold to the respective pyrethroids at the end of the 14th generation. Populations selected for resistance to one pyrethroid showed positive cross-resistance to all other pyrethroids, but no cross-resistance to endosulfan and thiodicarb. There was a significant increase in mixed-function oxidase activity with advancing generation suggesting its possible role in the positive cross-resistance among the pyrethroids. The induction of carboxyl esterases in pyrethroid-selected populations may have resulted in the activation of indoxacarb, thereby accounting for the observed negative cross-resistance. 相似文献
13.
The virulence of Helicoverpa armigera nucleopolyhedrovirus (HearNPV) to South African laboratory and field populations of Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) was evaluated. After determining the volume imbibed by each population, bioassays were performed using the droplet feeding method and gradient‐purified HearNPV occlusion bodies. Evaluation of dose‐ and time‐mortality data showed that the median lethal doses (LD50s) did not differ significantly between the populations but that the median lethal time (LT50) of the laboratory population was significantly shorter than the LT50s of the field populations. The study shows the importance of considering both the dose‐ and time‐mortality responses when comparing the response variability of populations to a nucleopolyhedrovirus infection. 相似文献
14.
光学活性拟除虫菊酯对棉铃虫神经细胞钠通道电流的影响 总被引:5,自引:2,他引:3
用全细胞膜片钳技术对比分析了alpha体氯氰菊酯与theta体氯氰菊酯对棉铃虫Helicoverpa armigera幼虫离体培养中枢神经细胞Na+通道门控过程的影响。结果表明,alpha体氯氰菊酯作用后,神经细胞Na+通道电流(INa)先增大,同时通道的激活电压向负电位方向移动约10 mV,提示alpha体氯氰菊酯使通道激活电位降低,通道更容易被激活。药剂作用约10 min后,INa又迅速降低,表明alpha体氯氰菊酯对开放状态的Na+通道有抑制作用。另外,alpha体氯氰菊酯使INa到达峰值的时间缩短,但对失活时间无明显影响。Theta体氯氰菊酯也使INa激活电位左移,幅值降低,但降低速率较慢。总的结果表明alpha体氯氰菊酯与theta体氯氰菊酯对棉铃虫中枢神经细胞处于关闭和开放状态的钠通道均有作用,且alpha体氯氰菊酯对钠通道电流的抑制作用强于theta体氯氰菊酯。 相似文献
15.
利用放射化学的方法分别检测了棉铃虫Helicoverpa armigera、粘虫Mythimna separata幼虫和成虫肠中咽侧体静止激素(allatostatin, AS)样的活性物质。发现在棉铃虫、粘虫幼虫和成虫肠中均存在的AS样活性物质,可以在体外抑制咽侧体(corpora allata, CA)的保幼激素(juvenile hormone, JH)的生物合成。生物测定的结果表明,粘虫幼虫肠中AS样活性物质的含量较棉铃虫的高;粘虫1个幼虫肠当量对CA的JH合成的抑制率达43%,而棉铃虫幼虫肠只有26%。无论是棉铃虫还是粘虫,雌成虫中肠对CA的抑制比雄成虫中肠的高,后肠对CA的JH合成的抑制明显的低于中肠对CA的抑制。中肠对CA的JH合成的抑制是可回复的。中肠粗提物经蛋白酶水解后对CA合成JH的抑制率降低,表明肠中AS样的活性物质是肽或蛋白质。 相似文献
16.
利用放射化学的方法分别检测了棉铃虫Helicoverpaarmigera、粘虫Mythimnaseparata幼虫和成虫肠中咽侧体静止激素 (allatostatin ,AS)样的活性物质。发现在棉铃虫、粘虫幼虫和成虫肠中均存在的AS样活性物质 ,可以在体外抑制咽侧体 (corporaallata,CA)的保幼激素 ( juvenilehormone,JH)的生物合成。生物测定的结果表明 ,粘虫幼虫肠中AS样活性物质的含量较棉铃虫的高 ;粘虫 1个幼虫肠当量对CA的JH合成的抑制率达 4 3% ,而棉铃虫幼虫肠只有 2 6%。无论是棉铃虫还是粘虫 ,雌成虫中肠对CA的抑制比雄成虫中肠的高 ,后肠对CA的JH合成的抑制明显的低于中肠对CA的抑制。中肠对CA的JH合成的抑制是可回复的。中肠粗提物经蛋白酶水解后对CA合成JH的抑制率降低 ,表明肠中AS样的活性物质是肽或蛋白质 相似文献
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18.
2005年在北疆棉区棉铃虫Helicoverpa armigera2代成虫期田间试验表明,水盆诱捕器每个每晚平均诱捕头数是饮水瓶的6.3倍(瓶竖放)和18.4倍(瓶斜放),差异均达显著水准(P=0.05)。比较单个诱捕器1晚最大诱蛾数,水盆是饮水瓶的8.0倍(瓶竖放)和24.0倍(瓶斜放),差异均达显著水准(P=0.05)。比较逐日平均诱蛾数,水盆是饮水瓶的9.44倍,差异达极显著水准(P=0.01)。综合考虑经济成本,在此条件下用饮水瓶制作棉铃虫诱捕器是不可取的。 相似文献
19.
棉铃虫卵在不同生育期玉米上的分布 总被引:2,自引:0,他引:2
1998年在江苏丰县系统调查了棉铃虫卵在不同生育期玉米上的垂直分布和水平分布。表明棉铃虫在抽雄扬花期以前的玉米上产卵 ,2代期间叶片正面占总卵量的 93 5% ,茎杆上占 6 5% ,3代期间叶片正面占 89 5% ,茎杆上占 1 0 5% ;抽雄扬花期主要产在雄穗和新鲜的雌蕊花丝上 ,分别占 59 1 %和2 6 9% ,叶片正面和茎杆上分别只占 1 1 1 %和 2 9%。抽雄扬花后有 97 5%的卵产在雌蕊花丝上 ,叶片和茎杆上为 0 5%和 2 0 %。第 3代棉铃虫卵期 ,抽雄扬花期的玉米对卵的分流作用约为抽雄扬花前玉米的 3倍 ,抽雄扬花后的玉米对卵的分流作用最小。 相似文献
20.
Effect of jasmonic acid and salicylic acid induced resistance in groundnut on Helicoverpa armigera 总被引:1,自引:0,他引:1
Induced resistance in plants affects insect growth and development as a result of the up‐regulation of defence‐related secondary metabolites or enzyme‐binding proteins. In the present study, the effects of jasmonic acid (JA) and salicylic acid (SA) induced resistance in groundnut on Helicoverpa armigera (Hübner) are examined. Larval survival, larval weights and the activities of digestive enzymes (total serine protease and trypsin) and of detoxifying enzymes [glutathione S‐transferase (GST) and esterase (EST)] are studied in insects fed on four groundnut genotypes with moderate levels of resistance to H. armigera (ICGV 86699, ICGV 86031, ICG 2271 and ICG 1697) and a susceptible genotype (JL 24). The plants are pre‐ and/or simultaneously treated with JA and SA, and then infested with H. armigera, which are allowed to feed for 6 days. Significantly lower serine protease and trypsin activities are observed in H. armigera fed on plants treated with JA. Greater GST activity is recorded in insects fed on JA and SA treated plants, whereas EST activity is low in H. armigera larvae fed on plants treated with JA and SA. Serine proteases, trypsin and GST activities and larval weights (r = 0.74–0.95) and larval survival (r = 0.77–0.93) are positively correlated, whereas EST activity and larval weight (r = ?0.55) and larval survival (r = ?0.65) are negatively correlated. The results suggest that midgut digestive and detoxifying enzymes can be used as indicators of the adverse effects of constitutive and/or induced resistance in crop plants on the insect pests and the role of JA and SA in insect pest management. 相似文献