Physiological responses of the adult male collared peccary, Tayassu tajacu (Tayassuidae), to severe dietary restriction

Metabolic and hormonal responses of eight adult male collared peccaries (Tayassu tajacu) to an ad libitum diet intake, or 25% of an ad libitum intake, were examined. Blood samples for hematological, serum-biochemical and hormonal profiles were collected at three week intervals during the nine week experiment starting 4 August 1983. Males fed on the restricted diet lost an average of 26% of their body weight during the trial, compared to a slight weight gain for those fed ad libitum. Characteristics of the red and white blood cell populations were not influenced by diet intake, with the exception of mean corpuscular volume, which was consistently lower amongst males fed on the restricted diet. Restricted food intake resulted in significantly elevated serum values for urea nitrogen, urea nitrogen:creatinine, urea index, alpha globulin:beta globulin, gamma globulin:albumin, nonesterified fatty acids, alkaline phosphatase and lactate dehydrogenase isozymes (LD1 and LD2). Restricted food intake resulted in significantly lowered serum values for total alpha globulin, alpha-1 globulin, total beta globulin, beta-1 globulin, beta-2 globulin, glucose, triglycerides, calcium, magnesium, sodium, chloride, copper and triiodothyronine. Serum levels of creatinine, total protein, albumin, alpha-2 globulin, uric acid, total bilirubin, cholesterol, aspartate aminotransferase, alanine aminotransferase, gamma glutamyltransferase, lactate dehydrogenase, phosphorus, calcium:phosphorus, potassium, iron, zinc and thyroxine were unaffected by diet intake level. Semen evaluation indicated spermatogenesis was not affected by dietary restriction despite reductions in scrotal circumference and ejaculate gel volume. Serum testosterone levels were significantly lower among males fed on the restricted diet after nine weeks. These data suggest male libido might be depressed during poor range conditions, while maintenance of spermatogenesis might permit them to take immediate advantage of improved range conditions. Blood analysis of metabolic and hormonal function can provide useful information for predicting the adult male's nutritional and reproductive condition.     

血液灌流联合血液透析治疗尿毒症的临床价值分析

目的:探讨血液灌流联合血液透析治疗尿毒症的临床价值。方法:选取我院2014年5月~2016年4月接收血液透析的尿毒症患者90例,全部患者均采用血液透析进行维持性治疗,将其分为对照组(45例)与治疗组(45例)两组。对照组进行单纯的血液透析,而治疗组则在血液透析的基础上联合血液灌流进行治疗。观察和比较两组患者治疗前后血清肌酐(Cr)、β2微球蛋白(β2-MG)、血液尿素氮(BUN)、甲状旁腺激素(PTH)、球蛋白、白蛋白、血常规、电解质的变化情况以及治疗后临床症状的改善情况。结果:治疗前,两组患者的血清Cr、β2-MG、BUN、PTH水平比较差异无统计学意义(P0.05);治疗后,治疗组患者的血清β2-MG、PTH水平较治疗前明显下降(P0.05),对照组患者各项指标无明显变化(P0.05)。两组患者治疗前后的球蛋白、白蛋白、血常规、电解质均无明显变化。治疗后,治疗组的临床不良反应改善率明显高于对照组(P0.05)。结论:与单纯的血液透析相比,血液灌注联合血液透析能够更有效清除尿毒症患者体内Cr、β2-MG、BUN、PTH的蓄积,改善不良反应,且不会引起球蛋白、白蛋白、血常规、电解质的变化而破坏内平衡。     

Relationship between seasonal energy economy and thyroid activity in farm-raised raccoon dogs

1. The levels of thyroid hormones (T3, T4), total lipids and urea in blood serum of adult farmed raccoon dogs (Nyctereutes procyonoides Gray 1834) were monitored year-round, and compared with seasonal changes in body weight and feed consumption during intense, maintenance and restricted fasting feeding. 2. Thyroid hormone levels tended to be low during winter whereas during the rest of the year no marked seasonal differences were observed. That thyroid hormone levels were influenced by altering feed intake suggests the hypothesis that the winter hypothyroidism observed was a result of a decreased level of voluntary feed intake. 3. The colder the temperature was during winter, the less the animals consumed the feed supplied. 4. Neither season nor feed intake level affected serum levels of total lipids or urea. 5. Marked seasonal changes in body weight of the animals were found. From maximum values occurring in early winter their body weight generally dropped about 30% reaching the minimum values in mid-summer. This marked seasonal change was mainly the result of changes in subcutaneous fat reserves.     

Ten-Year-Replicated Circadian Profiles for 36 Physiological, Serological and Urinary Variables in Healthy Men

At 3-hr intervals over a 24-hr span, 36 systemic, serologic and urinary variables were examined in 7 men in their mid 20's in the Spring of 1969, and again in the same 7 men in the Spring of 1979 under a similar chronobiologic protocol, using the same chemical and numerical analytical procedures. The variables examined for rhythms by cosinor were: vital signs--blood pressure (systolic, diastolic, pulse pressure and mean arterial pressure), heart rate, intraocular pressure (left and right), oral temperature; serum components--albumin, albumin/globulin ratio, total bilirubin, calcium, carbon dioxide, chlorides, bilirubin, cholesterol, globulin, glucose, potassium, sodium, sodium/potassium ratio, transaminase, triglycerides, total protein, urea nitrogen; and urine components--calcium, calcium/magnesium ratio, creatinine, magnesium, pH, potassium, sodium, sodium/potassium ratio, urea clearance, urea nitrogen, volume and zinc. Although all subjects appeared clinically healthy in 1969 and in 1979, certain inter-study differences were observed in a number of rhythm parameters of different variables. Statistically significant increases in mesor for the group as a whole were observed for serum Ca, cholesterol, Cl, CO2, K, Na, and while statistically significant mesor decreases for a group as a whole were noted in serum glucose and transaminase. Statistically significant increases in amplitude for the group as a whole were observed in serum chloride and urinary Na/K ratio, while statistically significant decreases were observed in amplitude for blood pressure, heart rate, serum albumin, A/G ratio, globulin, glucose, protein, sodium and transaminase.(ABSTRACT TRUNCATED AT 250 WORDS)     

Circannual Variations in Baseline Blood Values of Dogs

Forty-two hematological and biochemical variables routinely measured in dogs as part of a preoperative protocol have been analyzed for circannual changes by analysis of variance (ANOVA) and single cosinor procedures. Data were available from up to 489 adult mongrel dogs of both sexes studied on weekdays over a 5-year span (January 5, 1987 to December 18, 1991). Dogs were housed in individual cages at 24 ± 1 °C with dog chow and tap water available ad libitum and lights on between 06:00 and 18:00 h. A single blood sample/dog was collected by jugular venipuncture between 08:00 and 09:00 h and sent to a commercial laboratory for hematological and biochemical determinations. Data were assigned to date and time of sampling and analyzed for the effect of time of year by ANOVA (across 12 months and 4 seasons), and by the least-squares fit of a precise 1-year cosine. ANOVA and single cosinor described a significant circannual time effect and rhythm for the following: total leukocytes, lymphocytes, hemoglobin, mean corpuscular hemoglobin (MCH), MCH concentration, red cell distribution width, mean platelet volume, creatinine, blood urea nitrogen (BUN), BUN/crea-tinine ratio, amylase, glucose, chloride, uric acid, direct bilirubin, total protein, albumin, globulin, albumin/globulin ratio, lactate dehydrogenase, alkaline phos-phatase, γ-glutamyltransferase, alanine aminotransferase (ALT), and aspartate aminotransferase (AST)/ALT ratio. A significant effect of season by ANOVA only was found for: Ca, Na, phosphorus, total bilirubin, hematocrit, mean corpuscular volume, and neutrophils. No significant time effect could be found at p n%ge 0.05 by either statistical method for: K, Mg, Fe, cholesterol, triglycerides, ASP, red blood cells, monocytes, eosinophils, basophils, or platelets. Acrophases occurred for the most part in either the winter or summer.     

实验藏酋猴日粮蛋白质营养水平研究

目的本试验旨在研究不同蛋白水平对实验藏酋猴生长性能和血液生化指标的影响并确定幼年实验藏酋猴的蛋白营养需要量。方法选用15只幼年实验藏酋猴随机分为5个处理组,每个处理3个重复,分别饲喂蛋白水平为11．1％、16．1％、20．5％、24．5％和29．8％的日粮,试验期90d。结果随着日粮中蛋白水平的提高,实验藏酋猴的增重、血清白蛋白、球蛋白、天门冬氨酸氨基转移酶、丙氨酸氨基转移酶和尿素氮含量显著增加（P〈0．05）,而血清总蛋白、甘油三酯、γ-谷氨酰胺转移酶和碱性磷酸酶无显著差异。应用折线法确定幼年实验藏酋猴的蛋白营养需要量为23．69％。结论日粮蛋白水平对实验藏酋猴生长性能有显著影响,从生物学和经济学角度考虑,初步认为在本试验条件下,未成年实验藏酋猴日粮蛋白水平以23．69％最合适。     

Circannual Variations in Baseline Blood Values of Dogs

Forty-two hematological and biochemical variables routinely measured in dogs as part of a preoperative protocol have been analyzed for circannual changes by analysis of variance (ANOVA) and single cosinor procedures. Data were available from up to 489 adult mongrel dogs of both sexes studied on weekdays over a 5-year span (January 5, 1987 to December 18, 1991). Dogs were housed in individual cages at 24 ± 1 °C with dog chow and tap water available ad libitum and lights on between 06:00 and 18:00 h. A single blood sample/dog was collected by jugular venipuncture between 08:00 and 09:00 h and sent to a commercial laboratory for hematological and biochemical determinations. Data were assigned to date and time of sampling and analyzed for the effect of time of year by ANOVA (across 12 months and 4 seasons), and by the least-squares fit of a precise 1-year cosine. ANOVA and single cosinor described a significant circannual time effect and rhythm for the following: total leukocytes, lymphocytes, hemoglobin, mean corpuscular hemoglobin (MCH), MCH concentration, red cell distribution width, mean platelet volume, creatinine, blood urea nitrogen (BUN), BUN/crea-tinine ratio, amylase, glucose, chloride, uric acid, direct bilirubin, total protein, albumin, globulin, albumin/globulin ratio, lactate dehydrogenase, alkaline phos-phatase, γ-glutamyltransferase, alanine aminotransferase (ALT), and aspartate aminotransferase (AST)/ALT ratio. A significant effect of season by ANOVA only was found for: Ca, Na, phosphorus, total bilirubin, hematocrit, mean corpuscular volume, and neutrophils. No significant time effect could be found at p n%ge 0.05 by either statistical method for: K, Mg, Fe, cholesterol, triglycerides, ASP, red blood cells, monocytes, eosinophils, basophils, or platelets. Acrophases occurred for the most part in either the winter or summer.     

Seasonal variations in physiological indices of adult female white-tailed deer in Texas

Seasonal variations in blood chemistry, urine chemistry, fat reserves, and crude protein levels of rumen contents were determined for free-ranging adult female white-tailed deer (Odocoileus virginianus Zimmermann) in central Texas. Seasonal variations (P less than 0.05) existed for serum total protein, albumin, globulin, albumin/globulin ratios, blood urea nitrogen (BUN), cholesterol, alkaline phosphatase, creatinine, phosphorus, and sodium; and urinary urea/creatinine (U/C) ratios, rumen crude protein, the kidney fat index (KFI), femur marrow fat (FMF), and dressed weights. Variations in BUN, urinary U/C ratios, dressed weights, KFI, and FMF were attributed partially to the nutritional demands of late gestation and lactation.     

笼养绿尾虹雉血液生理生化指标分析

为了解人工饲养绿尾虹雉(Lophophorus lhuysii)血液生理生化指标及特点,以四川宝兴蜂桶寨国家级自然保护区笼养的9只成年非繁殖期绿尾虹雉为研究对象,测定了34项血液生理生化指标,按性别分组统计进行差异显著性检验。结果表明,绿尾虹雉血液生理指标不同性别间无显著差异(P0.05)。血液生化指标中的血液总蛋白雄性显著低于雌性(P0.05),血清尿素雄性显著高于雌性(P0.05),但血液中的其他有机物(白蛋白、球蛋白、肌酐、葡萄糖、尿素、酶、总胆固醇、甘油三酯等)及无机离子(钾、钠、镁、氯、钙、磷、铁)不同性别之间无显著差异(P0.05)。说明本实验条件下,笼养绿尾虹雉非繁殖期血液生理指标与性别没有相关性,血清总蛋白、血清尿素含量可能与性别有关。     

Hematology and serum chemistry of cottontail rabbits of southern Illinois.

In 1983 and 1984 blood was collected from 79 cottontail rabbits (Sylvilagus floridanus) confined to an outdoor enclosure in southern Illinois to establish reference values for hematology and serum chemistry. Packed cell volume, sodium, potassium, chloride, glucose, calcium, carbon dioxide, blood urea nitrogen, creatinine, uric acid, cholesterol, albumin, bilirubin, alkaline phosphatase, aspartate transaminase, alanine aminotransaminase, total protein, albumin/globulin ratio, and osmolality were measured. Sex and age (adult versus juvenile) of rabbit as well as season (June to September versus October to May) and method of capture (trap versus shot) variously affected most hematology and serum chemistry variables.     

Effects of living at two ambient temperatures on 24-h blood pressure and neuroendocrine function among obese and non-obese humans: a pilot study

The effects of environmental temperature on blood pressure and hormones in obese subjects in Japan were compared in two seasons: summer vs winter. Five obese (BMI, 32?±?5 kg/m²) and five non-obese (BMI, 23 ±3 kg/m²) men participated in this experiment at latitude 35°10′ N and longitude 136°57.9′ E. The average environmental temperature was 29?±?1 °C in summer and 3?±?1 °C in winter. Blood samples were analyzed for leptin, ghrelin, catecholamines, thyroid stimulating hormone (TSH), free thyroxine (fT₄), free triiodothyronine (fT₃), total cholesterol, triglycerides, insulin and glucose. Blood pressure was measured over the course of 24 h in summer and winter. A Japanese version of the Profile of Mood States (POMS) questionnaire was also administered each season. Systolic and diastolic blood pressures in obese men were significantly higher in winter (lower environmental temperatures) than in summer (higher environmental temperatures). Noradrenaline and dopamine concentrations were also significantly higher at lower environmental temperatures in obese subjects, but ghrelin, TSH, fT₃, fT₄, insulin and glucose were not significantly different in summer and winter between obese and non-obese subjects. Leptin, total cholesterol and triglyceride concentrations were significantly higher in winter in obese than non-obese men. Results from the POMS questionnaire showed a significant rise in Confusion at lower environmental temperatures (winter) in obese subjects. In this pilot study, increased blood pressure may have been due to increased secretion of noradrenaline in obese men in winter, and the results suggest that blood pressure control in obese men is particularly important in winter.     

Seasonal and circadian fluctuations of the biochemical indices of the blood in mice

Seasonal (in January, April, July, October) changes of aspartate aminotransferase (AST), alanine aminotransferase (ALT), protein, bilirubin, glucose, cholesterol, creatinine, blood urea nitrogen, Cl-, K+, Na+ content were studied in the blood plasma of mice at different time of day (6 p. m., midnight, 6 a. m., midday). The analysis of the average daily indices has shown that the most expressed variations were the following: AST (spring maximum is 3.7 times higher than autumn minimum), ALT (winter maximum is 2.9 times higher than autumn minimum), creatinine (summer maximum is 2.5 times higher than winter minimum), blood urea nitrogen (summer maximum is 2.5 times higher than autumn minimum). Bilirubin and protein content in spring is insignificant, but it is significantly higher than in other seasons. Cholesterol content is lower in winter. No differences in glucose, Cl-, K+, Na+ content in different months have been revealed. The largest circadian synchronization was observed in winter in AST, glucose, cholesterol, protein, Cl-, K+, Na+ (the level observed at 6 p. m. and at midday is higher than that observed at midnight and 6 a. m.) and in autumn in AST, ALT, glucose, cholesterol, blood urea nitrogen, with the circadian curves inverse as compared to the winter period. In spring practically no circadian synchronization was observed.     

赤腹松鼠血清肝肾功能指标的测定

以捕自四川荥经县的161只成体赤腹松鼠(Callosciurus erythraeus,雄鼠76只,雌鼠85只)为研究对象,从2015年2月至12月分四个季节测定了雌、雄鼠血清中反映肝肾功能的14项指标,并分析了妊娠对各项指标的影响。结果显示:(1)肝肾功能指标均无显著的性别差异。(2)白蛋白、胆碱酯酶和肌酐在春季有升高的趋势,但无明显的季节差异。(3)总蛋白、球蛋白及尿酸含量春季低于其他季节,总胆红素、直接胆红素、间接胆红素和尿素氮含量春季高于其他季节,前白蛋白、丙氨酸氨基转移酶、天冬氨酸氨基转移酶和γ-谷氨酰转移酶春季高于其他季节,夏季显著下降,冬季最低。(4)妊娠鼠血清胆碱酯酶含量高于未妊娠鼠,尿素氮、肌酐和尿酸含量低于未妊娠鼠。结果表明,雌、雄赤腹松鼠肝肾功能指标无性别差异且季节变化趋势相同,大多数在春季变化显著且妊娠影响较小,这可能与雌、雄的繁殖状态及在多变性环境条件下的生理适应能力有关。     

Effect of Mineral Fortification on Plasma Biochemical Profile in Rats

This study aimed at investigating the changes in biochemical profile of male rats following 8 weeks administration of different concentration of elemental iron, sodium iron ethylenediaminetetraacetate (NaFeEDTA), zinc sulfate (ZnSO₄), and zinc oxide (ZnO) in whole wheat flour. Eight groups comprising five rats each were fed fortified whole wheat flour in the form of baked pallets, while one group served as control. Concentration of total cholesterol, high density lipoprotein-cholesterol (HDL-C), low density lipoprotein-cholesterol (LDL-C), triglycerides, total proteins, albumin, globulin, plasma glucose, and blood urea nitrogen were assayed. Supplementing mineral-fortified diet to male rats did not indicate any significant (p ≤ 0.05) effect on total cholesterol concentration. Diets containing NaFeEDTA alone increased HDL-C and decreased LDL-C; however, the differences remained non significant. Likewise, plasma triglycerides content of male rats remained unchanged on feeding fortified diets. Diets containing iron as NaFeEDTA and elemental iron exerted little effect on total protein concentration in the plasma of rats. Plasma glucose and blood urea nitrogen levels did not exhibit any significant change as a result of ingesting mineral supplemented diets. The study concludes that the forms of fortificants and the fortification levels used in the current study are undamaging for lipid profile, renal function, and glucose levels in rats, suggesting that these may be safely used in wheat flour to combat iron and zinc deficiency in vulnerable groups.     

Serum and urine biochemical indicators of nutritional status in adult female collared peccaries,Tayassu tajacu (tayassuidae)

• 1.1. Physiological responses of 13 adult female collared peccaries (Tayassu tajacu) to high quality and low quality diets, fed for 15 weeks, were examined. The low quality diet simulated energy and protein intake of peccaries during poor range conditions resulting from drought. Blood samples were collected after 10 and 15 weeks of dietary treatment; urine samples were collected after 15 weeks of treatment.
• 2.2. Females receiving the low quality diet for 15 weeks lost 27.4% of their original body weight, compared to no weight change among high quality-fed females.
• 3.3. Red blood cell counts, hematocrits, and hemoglobin concentrations were significantly greater among females fed a high quality diet compared to those receiving a low quality diet. High quality-fed females also had a higher mean corpuscular hemoglobin concentration. Plasma fibrinogen concentration was nearly twice as great among females receiving the low quality diet compared to the high quality group.
• 4.4. Consumption of the low quality diet resulted in significantly elevated serum levels of nonesterified fatty acids, alkaline phosphatase, phosphorus, alpha-2 globulin and alpha globulin: beta globulin ratio.
• 5.5. Consumption of the low quality diet resulted in significantly lowered serum levels of urea nitrogen, calcium, zinc, calcium: phosphorus, urea index, beta-1 flobulin, beta globulin: albumin ratio, thyroxine and triiodothyronine.
• 6.6. Serum levels ofcreatinine, total bilirubin, glucose, cholesterol, gamma glutamyltransferase, aspartate aminotransferase, alanine aminotransferase, lactate dehydrogenase, potassium, copper, magnesium, sodium chloride, total protein and gamma globulin were unaffected by diet quality.
• 7.7. Urine chemistry results suggested pH, osmolarity, albumin, creatinine phosphokinase, calcium and phosphorus concentrations might be useful indices for assessing nutritional status in female peccaries.

     

老年原发性高血压患者血压晨峰与早期肾损害的临床观察

目的:探讨老年原发性高血压患者血压晨峰与早期肾损害的关系。方法:选择我院收治的94例老年原发性高血压患者进行24小时动态血压监测,并根据监测结果,将患者分为晨峰组62例和非晨峰组32例,检测和比较两组的血肌酐和尿素氮、12小时尿微量蛋白、尿β2微球蛋白、空腹血糖、血脂等指标。结果:老年高血压患者晨峰组12小时尿微量蛋白、尿β2微球蛋白均显著高于非晨峰组(P0.05);晨峰血压与12小时尿微量白蛋白(r=0.374)、尿β2微球蛋白(r=0.456)呈显著正相关。结论:老年原发性高血压患者血压晨峰与早期肾损害有关,治疗高血压的同时重视控制晨峰血压有重要意义。     

Effects of anabolic steroids on acute phase responses in intra-abdominal sepsis

The acute phase response is an important adaptive response to sepsis and injury. As anabolic steroids increase protein synthesis we postulated that these agents might also increase hepatic acute phase protein synthesis. Male Wistar rats were pretreated with testosterone or danazol for 48 h prior to caecal ligation and puncture (CLP). Thirty-six h following surgery the animals were killed and blood taken for full blood count, total protein, albumin, alpha, beta and gamma globulin fractions on serum electrophoresis, complement C(3) and transferrin levels. Danazol increased the alpha1, alpha2 and beta1 globulin serum protein fractions in comparison with no surgery and CLP alone groups. These results indicate that danazol increases plasma acute phase proteins, as measured by electrophoresis, in this model of intra-abdominal sepsis.     

扎龙自然保护区雌性丹顶鹤血液生化指标

2004年11月至2005年9月,采用自动生化分析仪对扎龙自然保护区10只雌性成体丹顶鹤在越冬期、繁殖期和迁徙期的20项血液生化指标（总蛋白、白蛋白、球蛋白、直接胆红素、间接胆红素、总胆红素、血糖、尿素氮、肌酐、总胆固醇、甘油三酯、谷草转氨酶、谷丙转氨酶、碱性磷酸酶、肌酸肌酶、羟丁酸脱氢酶、乳酸脱氢酶、钙离子、无机磷和镁离子）进行了分析.结果表明：除总蛋白、球蛋白和血糖外,研究区雌性丹顶鹤血液中的其他指标均在不同时期之间表现出显著（P<0.05）或极显著差异（P<0.01).说明总蛋白、球蛋白和血糖可作为雌性丹顶鹤血液生化指标的可靠参考值,其他17项血液生化指标的年变化动态体现出雌性丹顶鹤在不同生理时期的生理特征和生态适应性.如果以这17项血液生化指标作为雌性丹顶鹤血液生化指标的参考值,就必须考虑不同生理时期对其含量的影响.     

Glycerol metabolism in the hibernating black bear

Summary The contribution of glycerol to protein, carbohydrate, and lipid metabolism was studied in black bears. Special attention was directed at the role of glycerol in preventing uremia. In summer and winter, U-¹⁴C glycerol, as well asl(U¹⁴C)-alanine andd(U-¹⁴C)-glucose were injected intravenously; timed sampling of venous blood and expiratory gases was made.In winter, during hibernation,¹⁴C-label from glycerol was found in alanine and other free amino acids and plasma proteins, pyruvate, lactate, glucose and lipid esters. After 48 h, most of the¹⁴C-label in plasma was found in proteins. However, during the four days of study, no label was found in serum urea. Similar results occurred in summer except, in marked contrast to winter,¹⁴C-labeled urea was continually detected in blood. Expired¹⁴C-CO₂ was considerably lower in winter than summer and winter respiratory quotient was 0.69.In both summer and winter,¹⁴C-labeled alanine also entered plasma proteins and generated pyruvate, lactate and glucose. Once again the marked difference between active and dormant phases was demonstrated: There was no labeled plasma urea in winter while it was continually detected in summer. ¹⁴C-labeled glucose experiments revealed very slow carbohydrate metabolism in winter.These findings suggest that in winter, glycerol helps prevent uremia by serving as a carbon source for amino acid formation. The nitrogen involved in these reactions is thus diverted from urea synthesis into protein synthetic pathways. Further, glycerol also appears to serve as an active substrate for gluconeogenesis and lipogenesis in hibernation.     

基于高通量测序的缢蛏及其养殖池塘菌群结构的季节变化

为探究缢蛏养殖过程中养殖环境和缢蛏体内的细菌群落结构季节变化特征及其与环境因子的相关性,采用高通量测序技术对缢蛏养殖池塘的水体、沉积物及缢蛏内脏团细菌菌群进行了研究.结果表明: 水体中冬季的细菌菌群结构与其他3季差异显著,沉积物和缢蛏内脏团4季的细菌菌群结构均无显著差异.水体各季节细菌群落的Shannon多样性指数无明显差异,沉积物及缢蛏内脏团细菌群落Shannon多样性指数夏季最低,冬季最高.门水平上,蓝细菌门、变形菌门和拟杆菌门为水体的优势菌群;变形菌门、绿弯菌门和拟杆菌门为沉积物的优势菌群;柔膜菌门和变形菌门为缢蛏内脏团的优势菌群.属水平上,水体冬季的优势菌属为NS3a_marine_group,而其余3季为聚球藻属;沉积物优势菌属为厌氧绳菌科下的一类菌属norank_f_Anaerolineacea和硝化螺旋菌属;内脏团优势菌属为支原体属和弓形菌属.环境因子与菌群的相关性分析表明: 水体的优势菌属聚球藻属与水温、化学需氧量(COD)、PO₄^--P、NH₄⁺-N、pH和透明度呈正相关;沉积物的优势菌属厌氧绳菌科菌属norank_f_Anaerolineacea与COD、水温、总磷呈正相关;缢蛏内脏团的优势菌属支原体属与水温、pH、NH₄⁺-N、PO₄^--P和透明度呈正相关.这表明不同季节的缢蛏养殖池塘及缢蛏体内细菌群落结构及多样性差异较大,水体细菌菌群受养殖环境的影响明显,尤其是水温及氮磷含量.