三叶斑潜蝇发生特点研究

2007—2009年在浙江杭州对三叶斑潜蝇的发生情况进行了调查。结果表明,在杭州地区范围内该虫的分布已很广,与其他斑潜蝇混合发生。春夏季以美洲斑潜蝇为害为主,6月是为害盛期。秋冬季为多种斑潜蝇混合为害,9月是混发盛期,期间以三叶斑潜蝇为害占多数。目前查到有13种寄主作物受三叶斑潜蝇为害。     

南美斑潜蝇对不同黄瓜品种的寄主选择性

采用自由选择法测定了南美斑潜蝇Liriomyza huidobrensis对黄瓜不同品种的寄主选择性。结果表明,南美斑潜蝇成虫产卵和取食选择性最强的黄瓜品种为冬棚王,其对冬棚王的选择性与对光和地黄瓜、新四号黄瓜、津优2号、双杂一号、津杂2号、冬雪王、早春王、神农春四号、津春4号等品种的选择性存在显著差异,而与山东密刺、津优1号、春四号黄瓜、津杂4号、鲁优二号、津优4号、唐山秋瓜、神农春五号、津绿4号等品种差异不显著。测定结果表明,黄瓜不同品种间的叶毛数、可溶性蛋白质及可溶性糖含量存在显著差异。相关分析表明,南美斑潜蝇对不同黄瓜品种的寄主选择性与其叶毛数存在显著的负相关,相关系数r=-0.475 5(P=0.039 5<0.05),而与叶片的可溶性蛋白质及可溶性糖含量关系不显著。     

寄主挥发物、叶色和表皮毛在美洲斑潜蝇寄主选择中的作用

在室内条件下 ,初步研究了寄主挥发物、叶色和表皮毛在美洲斑潜蝇寄主选择中的作用。在嗅觉仪试验中 ,寄主叶片挥发物对美洲斑潜蝇雌成虫没有明显的引诱作用 ;在叶色反应试验中 ,美洲斑潜蝇雌成虫在叶子圆片上停留的时间明显大于在滤纸圆片上停留的时间 (p<0 .0 1) ,其在有叶片区域分布的数量明显多于空白对照 (p <0 .0 1) ;在表皮毛试验中 ,美洲斑潜蝇在无毛叶片上的产卵量明显大于在有毛叶片上的产卵量 (p <0 .0 1)。上述结果表明 ,在对寄主的定向和定位过程中 ,美洲斑潜蝇的视觉起着重要的作用 ,而嗅觉不起作用 ;叶片表皮毛有抑制产卵的作用     

江苏泰兴市首次发现三叶草斑潜蝇疫情

2008年7月初泰兴市植物检疫站在泰兴市泰兴镇蔬菜基地发现并采集带有斑潜蝇潜道的药芹叶片,通过对其进行形态特征和分子生物学鉴定,确认为江苏地区首次发现检疫性害虫三叶草斑潜蝇Liriomyza trifolii(Burgess)。通过黄板诱集成虫和田间寄主作物调查,对泰兴市2008-2009年发生特征及年度间、寄主间为害情况差异进行分析,并针对今后加强三叶草斑潜蝇监测与防治工作中存在的问题提出设想与建议。     

三叶草斑潜蝇和美洲斑潜蝇的分子鉴定

以三叶草斑潜蝇Liriomyzatrifolii(Burgess)与美洲斑潜蝇LiriomyzasativaeBlanchand为材料,通过特异引物18SP1和5.8SP2,分别扩增出它们的rDNA非编码区ITS1的片段,根据序列分析和比较结果,重新设计出2条新的特异性引物Fly3和Flyus,并筛选到2个特异性内切酶,通过PCR和PCR产物的酶切分析,即可将这2种近缘昆虫区分开来。     

南美斑潜蝇寄主选择性与植物营养物质及叶绿素含量的关系

采用非自由选择法,在室内研究了南美斑潜蝇寄主选择性及其与植物叶片营养物质和叶绿素含量的关系.结果表明,南美斑潜蝇成虫对不同供试植物的选择性存在显著差异,选择性最强的为菜豆、茼蒿和西葫芦,最差的为番茄和甘蓝.相关分析表明,南美斑潜蝇成虫寄主选择性与叶片可溶性糖含量存在显著的正相关关系(R＝0.5014,P=0.0174,F＝6.717),而与叶片可溶性蛋白和叶绿素含量相关关系不显著.     

六种植物叶片中葫芦素B对美洲斑潜蝇寄主选择性的影响

在网室条件下 ,测定了美洲斑潜蝇对豇豆、苦瓜、黄瓜、白瓜、节瓜和丝瓜的寄主选择性。结果表明 ,美洲斑潜蝇在 6种不同植物叶片上的幼虫数、虫道数和为害程度具有明显的差异。从幼虫数和为害程度综合分析 ,美洲斑潜蝇在豇豆和丝瓜上的幼虫数、为害程度最高 ,分别是 0 .183头 / cm2 ,13.4 7%和 0 .0 99头 / cm2 ,2 3.4 2 % ,表明该虫对豇豆和丝瓜的选择性最强。在苦瓜上没有美洲斑潜蝇的幼虫寄生及取食虫道。该虫对其它 3种植物的选择性依次为节瓜、白瓜和黄瓜。在此基础上 ,进一步研究了不同植物叶片甲醇提取物中葫芦素 B的含量与美洲斑潜蝇寄主选择性的关系。经高效液相色谱 (HPL C)测定 ,苦瓜中的葫芦素 B含量最高 (2 3.8μg/ ml) ,其次是黄瓜 (9.6 μg/ ml)、白瓜 (4 .3μg/ ml)、豇豆 (2 .7μg/ ml)和节瓜 (2 .5 μg/ m l) ,丝瓜中含量最少 ,仅为1.3μg/ ml。分析结果证实不同植物叶中的葫芦素 B含量与其幼虫数呈负相关 (r1 =- 0 .82 0 6 ,r0 .0 5=0 .811) ;将不同植物叶中的葫芦素 B含量和美洲斑潜蝇的为害程度分别转换成对数后进行相关性分析 ,二者之间的相关性呈极显著的负相关(r2 =- 0 .980 4 ,r0 .0 1 =0 .917)。说明随着寄主植物叶片中葫芦素 B含量的增加 ,美洲斑潜蝇的幼虫数及为害程度明显     

非嗜食寄主气味蛹期处理对不同寄主来源美洲斑潜蝇雌成虫嗅觉定向的影响

测定用菜豆和丝瓜饲养的美洲斑潜蝇Liriomyza sativae Blanchard蛹暴露于用正己烷稀释的番茄或南瓜叶片挥发性抽提物(TLE或PLE)72h后,羽化雌成虫在Y形嗅觉仪中对TLE或PLE的定向率、平均反应时间以及对正己烷的定向率和平均反应时间,比较幼虫寄主经历和寄主气味对美洲斑潜蝇成虫嗅觉记忆的影响。结果表明,蛹期使用非嗜食寄主植物气味处理,能增加美洲斑潜蝇羽化雌成虫对非嗜食寄主气味的定向;幼虫期的寄主经历对处理后的成虫嗅觉定向也产生影响,饲喂丝瓜的美洲斑潜蝇蛹期处理后,羽化雌成虫显著偏向选择处理气味而非正己烷,饲喂菜豆的美洲斑潜蝇对南瓜的气味也表现出同样的特点,但对番茄的气味则相反,即使处理后对番茄气味的选择比率增加,仍然偏向选择正己烷。     

南美斑潜蝇在不同寄主作物叶片上化蛹比例的初步调查

南美斑潜蝇Ｌｉｒｉｏｍｙｚａｈｕｉｄｏｂｒｅｎｓｉｓ(Ｂｌａｎ ｃｈａｒｄ)是近年传入甘肃省武威地区的危险性害虫。由于耐低温能力比美洲班潜蝇强 ,因此发生程度远较后者重 ,仅靠药剂防治幼虫和成虫很难迅速将其控制 ,于是人们提出同时进行土壤处理杀蛹的防治技术。为此 ,     

氮、磷、钾对美洲斑潜蝇寄主选择性的影响

研究了不同浓度的氮、磷、钾营养液处理豇豆叶对美洲斑潜蝇Liriomyza sativae寄主选择性的影响,并探讨了其可能的机理和应用。     

三叶草斑潜蝇的寄生蜂及其应用

本文就三叶草斑潜蝇Liriomyza trifolii(Burgess)的寄生蜂种类、生物学特性、寄主选择性和农药对寄生蜂的影响及寄生蜂在生物防治中的应用等方面的研究进展进行了综述。     

Cucurbitane Triterpenoid Oviposition Deterrent from Momordica charantia to the Leafminer,Liriomyza trifolii

Females of Liriomyza trifolii, a polyphagous leafminer, are often deterred from ovipositing on Momordica charantia leaves. The methanol extract of M. charantia leaves showed strong oviposition deterrent activity against L. trifolii females on the host plant leaf when it was dipped in the methanol extract at a concentration of 1 g of fresh leaf equivalent/ml. Bioguided fractionation of the methanol extract of the leaves of M. charantia led to the isolation of a new cucurbitane triterpenoid, 7,23-dihydroxy-3-O-malonylcucurbita-5,24-dien-19-al, along with another known compound, momordicine I. Both 7,23-dihydroxy-3-O-malonylcucurbita-5,24-dien-19-al and momordicine I respectively had significant ovipositing deterrent effect towards the adult females of L. trifolii on host plant leaves treated at concentrations of 3.25 and 33.60 μg/cm².     

Molecular identification of Liriomyza trifolii (Burgess) (Dipt., Agromyzidae) based on real-time PCR

Abstract:  Rapidly identifying juvenile individuals of Liriomyza trifolii (Burgess) from Liriomyza sativae Blanchard is crucial in plant quarantine. We report a molecular method to identify L . trifolii based on real-time polymerase chain reaction (PCR). By comparing partial DNA sequences of mitochondrial COI genes of L . trifolii samples collected from Guangdong and Taiwan provinces in China, Japan, Philippine, Israel, Germany, the USA, Mexico and Honduras sequenced by authors, and those of related species recorded in GenBank, a L . trifolii -specific probe was developed. There was no difference in individuals of different stages tested by this probe. The total time for real-time PCR assay system was 2 h, and it would save 3–7 h compared with conventional PCR.     

Development of a combined cold storage and methyl bromide fumigation treatment to control the American serpentine leaf miner Liriomyza trifolii (Diptera:Agromyzidae) in imported chrysanthemum cuttings

Individual chrysanthemum cuttings and leaves infested with eggs and larvae of the alien leaf miner Liriomyza trifolii, and batches of pupae, were treated in the laboratory to find an effective quarantine treatment to control the pest in imported cuttings. The laboratory tests were based on a statutory quarantine treatment of 2 days' cold storage at 1–2°C followed by methyl bromide fumigation at 15°C with a concentration time product (CTP) of 54 g h/m³, which is used to control Spodoptera littoralis on imported chrysanthemum cuttings. Treatments of 2 days' storage at 1–2°C followed by methyl bromide fumigation at 15°C with a range of CTPs were used to obtain accurate dose-response lines and estimate the LD99 and LD99 9 for each stage of L. trifolii. The largest estimates of the LD99 for eggs, larvae and pupae up to 3 days old were 40·04, 34·49 and 42·64g h/m³ respectively. These results indicate that the Spodoptera treatment should give high levels of kill of most stages of L. trifolii. However, the LD99 values for pupae more than 3 days old were greater than those for eggs, larvae and young pupae. Therefore if this treatment were adopted as a quarantine measure against L. trifolii, good prophylactic treatments and rigorous pre-packing inspections in exporting countries would need to be maintained, to minimise any risk of importing pupae. It would also be necessary to ensure that the temperature of the imported cuttings was raised uniformly to 15°C after cold storage and prior to fumigation. The practicality of the technique as a commercial treatment will depend on whether it is possible to achieve this without causing unacceptable phytotoxicity.     

Host finding and discrimination in Diglyphus isaea,a parasitoid of the chrysanthemum leaf miner,Chromatomyia syngenesiae

Naive and experienced Diglyphus isaea were attracted by host plant odours of lettuce and chrysanthemum to search and probe on infested and uninfested leaves. A slight preference was shown for leaves infested with Chromatomyia syngenesiae. At close range, visual stimuli were unnecessary for oviposition and host‐feeding. Contact with uninfested lettuce and chrysanthemum elicited searching and probing behaviour whereas host frass did not. Host larval movement appeared to aid host detection at close range. The number of ovipositor probes increased with proximity to live hosts but not for stationary, dead hosts. Dead hosts were frequently walked over or missed when D. isaea passed within 0.5 cm of the stationary larvae. Dead hosts were also rejected for oviposition but not for host feeding. Both naive and experienced females discriminated between healthy hosts and those which had been attacked by conspecifics or encountered previously.     

Is host selection in leafminer adults influenced by pre‐imaginal or early adult experience?

Abstract:  Olfactometer and feeding/oviposition preference tests were carried out to study if host plant preference in two agromyzid leafminers, Liriomyza trifolii and Liriomyza huidobrensis , is influenced by pre-imaginal or imaginal experience, and at what level (host plant species, host plant variety or host age). It was found that the choice of host plant species (potato or tomato) and host plant variety had a pre-imaginal component, which could be reinforced (or modified) by an imaginal component. On the other hand, preference for potato leaves of different ages did not appear to be based on either pre-imaginal or imaginal factors, but resulted from differential performance and oviposition preference. The Hopkins' host selection principle appears to operate in these leafminers at the level of host species, and to some extent at the level of host variety, but not with respect to host age.     

济南地区美洲斑潜蝇发生规律与防治

本研究了美洲斑潜蝇的发生规律,表明在济南地区露地菜田不能越冬,蔬菜大栅成为翌年的主要虫源地。试验了药剂处理土壤、药剂喷雾和药剂熏蒸太栅的防治效果,并探讨了推延大棚内西葫穆裁期的控害作用。