共查询到20条相似文献，搜索用时 0 毫秒
1.
2.
3.
4.
Ryoichi Araki Akiko Hasumi Osamu Ishizaki Nishizawa Katsunori Sasaki Ayuko Kuwahara Yuji Sawada Yasushi Totoki Atsushi Toyoda Yoshiyuki Sakaki Yimeng Li Kazuki Saito Toshiya Ogawa Masami Yokota Hirai 《Plant biotechnology journal》2013,11(8):1017-1027
Plants belonging to the Brassicaceae family exhibit species‐specific profiles of glucosinolates (GSLs), a class of defence compounds against pathogens and insects. GSLs also exhibit various human health–promoting properties. Among them, glucoraphanin (aliphatic 4‐methylsulphinylbutyl GSL) has attracted the most attention because it hydrolyses to form a potent anticancer compound. Increased interest in developing commercial varieties of Brassicaceae crops with desirable GSL profiles has led to attempts to identify genes that are potentially valuable for controlling GSL biosynthesis. However, little attention has been focused on genes of kale (Brassica oleracea var. acephala). In this study, we established full‐length kale cDNA libraries containing 59 904 clones, which were used to generate an expressed sequence tag (EST) data set with 119 204 entries. The EST data set clarified genes related to the GSL biosynthesis pathway in kale. We specifically focused on BoMYB29, a homolog of Arabidopsis MYB29/PMG2/HAG3, not only to characterize its function but also to demonstrate its usability as a biological resource. BoMYB29 overexpression in wild‐type Arabidopsis enhanced the expression of aliphatic GSL biosynthetic genes and the accumulation of aliphatic GSLs. When expressed in the myb28myb29 mutant, which exhibited no detectable aliphatic GSLs, BoMYB29 restored the expression of biosynthetic genes and aliphatic GSL accumulation. Interestingly, the ratio of methylsulphinyl GSL content, including glucoraphanin, to that of methylthio GSLs was greatly increased, indicating the suitability of BoMYB29 as a regulator for increasing methylsulphinyl GSL content. Our results indicate that these biological resources can facilitate further identification of genes useful for modifications of GSL profiles and accumulation in kale. 相似文献
5.
6.
7.
8.
Chinmayee Panda Xin Li Amanda Wager Han‐Yi Chen Xu Li 《The Plant journal : for cell and molecular biology》2020,102(6):1281-1293
9.
10.
11.
12.
13.
14.
15.
16.
Erwan Denis Nadia Kbiri Viviane Mary Gaëlle Claisse Natalia Conde e Silva Martin Kreis Yves Deveaux 《The Plant journal : for cell and molecular biology》2017,90(3):560-572
Procambial and cambial stem cells provide the initial cells that allow the formation of vascular tissues. WOX4 and WOX14 have been shown to act redundantly to promote procambial cell proliferation and differentiation. Gibberellins (GAs), which have an important role in wood formation, also stimulate cambial cell division. Here we show that the loss of WOX14 function phenocopies some traits of GA‐deficient mutants that can be complemented by exogenous GA application, whereas WOX14 overexpression stimulates the expression of GA3ox anabolism genes and represses GA2ox catabolism genes, promoting the accumulation of bioactive GA. More importantly, our data clearly indicate that WOX14 but not WOX4 promotes vascular cell differentiation and lignification in inflorescence stems of Arabidopsis. 相似文献
17.
Sofía Racca Elina Welchen Diana E. Gras Danuše Tarkowská Veronika Turečková Veronica G. Maurino Daniel H. Gonzalez 《The Plant journal : for cell and molecular biology》2018,94(1):105-121
We studied the effect of reducing the levels of the mitochondrial electron carrier cytochrome c (CYTc) in Arabidopsis thaliana. Plants with CYTc deficiency have delayed growth and development, and reach flowering several days later than the wild‐type but with the same number of leaves. CYTc‐deficient plants accumulate starch and glucose during the day, and contain lower levels of active gibberellins (GA) and higher levels of DELLA proteins, involved in GA signaling. GA treatment abolishes the developmental delay and reduces glucose accumulation in CYTc‐deficient plants, which also show a lower raise in ATP levels in response to glucose. Treatment of wild‐type plants with inhibitors of mitochondrial energy production limits plant growth and increases the levels of DELLA proteins, thus mimicking the effects of CYTc deficiency. In addition, an increase in the amount of CYTc decreases DELLA protein levels and expedites growth, and this depends on active GA synthesis. We conclude that CYTc levels impinge on the activity of the GA pathway, most likely through changes in mitochondrial energy production. In this way, hormone‐dependent growth would be coupled to the activity of components of the mitochondrial respiratory chain. 相似文献
18.
19.
Nayelli Marsch‐Martínez Pieter B. F. Ouwerkerk Jeanneth Pablo‐Villa Paulina Lozano‐Sotomayor Raffaella Greco Patricia Ballester Vicente Balanzá Suzanne J. H. Kuijt Annemarie H. Meijer Andy Pereira Cristina Ferrándiz Stefan de Folter 《The Plant journal : for cell and molecular biology》2014,80(1):69-81
20.