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OsASR5 enhances drought tolerance through a stomatal closure pathway associated with ABA and H2O2 signalling in rice
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Jinjie Li Yang Li Zhigang Yin Jihong Jiang Minghui Zhang Xiao Guo Zhujia Ye Yan Zhao Haiyan Xiong Zhanying Zhang Yujie Shao Conghui Jiang Hongliang Zhang Gynheung An Nam‐Chon Paek Jauhar Ali Zichao Li 《Plant biotechnology journal》2017,15(2):183-196
Drought is one of the major abiotic stresses that directly implicate plant growth and crop productivity. Although many genes in response to drought stress have been identified, genetic improvement to drought resistance especially in food crops is showing relatively slow progress worldwide. Here, we reported the isolation of abscisic acid, stress and ripening (ASR) genes from upland rice variety, IRAT109 (Oryza sativa L. ssp. japonica), and demonstrated that overexpression of OsASR5 enhanced osmotic tolerance in Escherichia coli and drought tolerance in Arabidopsis and rice by regulating leaf water status under drought stress conditions. Moreover, overexpression of OsASR5 in rice increased endogenous ABA level and showed hypersensitive to exogenous ABA treatment at both germination and postgermination stages. The production of H2O2, a second messenger for the induction of stomatal closure in response to ABA, was activated in overexpression plants under drought stress conditions, consequently, increased stomatal closure and decreased stomatal conductance. In contrast, the loss‐of‐function mutant, osasr5, showed sensitivity to drought stress with lower relative water content under drought stress conditions. Further studies demonstrated that OsASR5 functioned as chaperone‐like protein and interacted with stress‐related HSP40 and 2OG‐Fe (II) oxygenase domain containing proteins in yeast and plants. Taken together, we suggest that OsASR5 plays multiple roles in response to drought stress by regulating ABA biosynthesis, promoting stomatal closure, as well as acting as chaperone‐like protein that possibly prevents drought stress‐related proteins from inactivation. 相似文献
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Marcin Pieczynski Waldemar Marczewski Jacek Hennig Jakub Dolata Dawid Bielewicz Paulina Piontek Anna Wyrzykowska Dominika Krusiewicz Danuta Strzelczyk‐Zyta Dorota Konopka‐Postupolska Magdalena Krzeslowska Artur Jarmolowski Zofia Szweykowska‐Kulinska 《Plant biotechnology journal》2013,11(4):459-469
Developing new strategies for crop plants to respond to drought is crucial for their innovative breeding. The down‐regulation of nuclear cap‐binding proteins in Arabidopsis renders plants drought tolerant. The CBP80 gene in the potato cultivar Desiree was silenced using artificial microRNAs. Transgenic plants displayed a higher tolerance to drought, ABA‐hypersensitive stomatal closing, an increase in leaf stomata and trichome density, and compact cuticle structures with a lower number of microchannels. These findings were correlated with a higher tolerance to water stress. The level of miR159 was decreased, and the levels of its target mRNAs MYB33 and MYB101 increased in the transgenic plants subjected to drought. Similar trends were observed in an Arabidopsis cbp80 mutant. The evolutionary conservation of CBP80, a gene that plays a role in the response to drought, suggests that it is a candidate for genetic manipulations that aim to obtain improved water‐deficit tolerance of crop plants. 相似文献
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David Posé Itziar Castanedo Omar Borsani Benjamín Nieto Abel Rosado Ludivine Taconnat Albert Ferrer Liam Dolan Victoriano Valpuesta Miguel A. Botella 《The Plant journal : for cell and molecular biology》2009,59(1):63-76
Squalene epoxidase enzymes catalyse the conversion of squalene into 2,3‐oxidosqualene, the precursor of cyclic triterpenoids. Here we report that the Arabidopsis drought hypersensitive/squalene epoxidase 1‐5 (dry2/sqe1‐5) mutant, identified by its extreme hypersensitivity to drought stress, has altered stomatal responses and root defects because of a point mutation in the SQUALENE EPOXIDASE 1 (SQE1) gene. GC‐MS analysis indicated that the dry2/sqe1‐5 mutant has altered sterol composition in roots but wild‐type sterol composition in shoots, indicating an essential role for SQE1 in root sterol biosynthesis. Importantly, the stomatal and root defects of the dry2/sqe1‐5 mutant are associated with altered production of reactive oxygen species. As RHD2 NADPH oxidase is de‐localized in dry2/sqe1‐5 root hairs, we propose that sterols play an essential role in the localization of NADPH oxidases required for regulation of reactive oxygen species, stomatal responses and drought tolerance. 相似文献
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HrpN, a protein produced by the plant pathogenic bacterium Erwinia amylovora, has been shown to stimulate plant growth and resistance to pathogens and insects. Here we report that HrpN activates abscisic acid (ABA) signalling to induce drought tolerance (DT) in Arabidopsis thaliana L. plants grown with water stress. Spraying wild-type plants with HrpN-promoted stomatal closure decreased leaf transpiration rate, increased moisture and proline levels in leaves, and alleviated extents of damage to cell membranes and plant drought symptoms caused by water deficiency. In plants treated with HrpN, ABA levels increased; expression of several ABA-signalling regulatory genes and the important effector gene rd29B was induced or enhanced. Induced expression of rd29B, promotion of stomatal closure, and reduction in drought severity were observed in the abi1-1 mutant, which has a defect in the phosphatase ABI1, after HrpN was applied. In contrast, HrpN failed to induce these responses in the abi2-1 mutant, which is impaired in the phosphatase ABI2. Inhibiting wild-type plants to synthesize ABA eliminated the role of HrpN in promoting stomatal closure and reducing drought severity. Moreover, resistance to Pseudomonas syringae developed in abi2-1 as in wild-type plants following treatment with HrpN. Thus, an ABI2-dependent ABA signalling pathway is responsible for the induction of DT but does not affect pathogen defence under the circumstances of this study.Hong-Ping Dong and Haiqin Yu contributed equally to this study and are regarded as joint first authors. 相似文献
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Histone acetyltransferase GCN5 is essential for heat stress‐responsive gene activation and thermotolerance in Arabidopsis
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Zhaorong Hu Na Song Mei Zheng Xinye Liu Zhenshan Liu Jiewen Xing Junhua Ma Weiwei Guo Yingyin Yao Huiru Peng Mingming Xin Dao‐Xiu Zhou Zhongfu Ni Qixin Sun 《The Plant journal : for cell and molecular biology》2015,84(6):1178-1191
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Marek Marzec Agata Daszkowska-Golec Anna Collin Michael Melzer Kai Eggert Iwona Szarejko 《Plant, cell & environment》2020,43(9):2239-2253
Strigolactones (SLs) are a group of plant hormones involved in many aspects of plant development and stress adaptation. Here, we investigated the drought response of a barley (Hordeum vulgare L.) mutant carrying a missense mutation in the gene encoding the SL-specific receptor HvD14. Our results clearly showed that hvd14.d mutant is hyper-sensitive to drought stress. This was illustrated by a lower leaf relative water content (RWC), impaired photosynthesis, disorganization of chloroplast structure, altered stomatal density and slower closure of stomata in response to drought in the mutant compared to the wild type parent cultivar Sebastian. Although the content of abscisic acid (ABA) and its derivatives remained unchanged in the mutant, significant differences in expression of genes related to ABA biosynthesis were observed. Moreover, hvd14.d was insensitive to ABA during seed germination. Analysis of Arabidopsis thaliana mutant atd14-1 also demonstrated that mutation in the SL receptor resulted in increased sensitivity to drought. Our results indicate that the drought-sensitive phenotype of barley SL mutant might be caused by a disturbed ABA metabolism and/or signalling pathways. These results together uncovered a link between SL signalling and ABA-dependent drought stress response in barley. 相似文献
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Phytochrome B control of total leaf area and stomatal density affects drought tolerance in rice 总被引:3,自引:0,他引:3
We report that phytochrome B (phyB) mutants exhibit improved drought tolerance compared to wild type (WT) rice (Oryza sativa L. cv. Nipponbare). To understand the underlying mechanism by which phyB regulates drought tolerance, we analyzed root growth
and water loss from the leaves of phyB mutants. The root system showed no significant difference between the phyB mutants and WT, suggesting that improved drought tolerance has little relation to root growth. However, phyB mutants exhibited reduced total leaf area per plant, which was probably due to a reduction in the total number of cells per
leaf caused by enhanced expression of Orysa;KRP1 and Orysa;KRP4 (encoding inhibitors of cyclin-dependent kinase complex activity) in the phyB mutants. In addition, the developed leaves of phyB mutants displayed larger epidermal cells than WT leaves, resulting in reduced stomatal density. phyB deficiency promoted
the expression of both putative ERECTA family genes and EXPANSIN family genes involved in cell expansion in leaves, thus causing greater epidermal cell expansion in the phyB mutants. Reduced stomatal density resulted in reduced transpiration per unit leaf area in the phyB mutants. Considering all these findings, we propose that phyB deficiency causes both reduced total leaf area and reduced
transpiration per unit leaf area, which explains the reduced water loss and improved drought tolerance of phyB mutants. 相似文献
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Characterization of an activation‐tagged mutant uncovers a role of GLABRA2 in anthocyanin biosynthesis in Arabidopsis
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Xiaoyu Wang Xianling Wang Qingnan Hu Xuemei Dai Hainan Tian Kaijie Zheng Xiaoping Wang Tonglin Mao Jin‐Gui Chen Shucai Wang 《The Plant journal : for cell and molecular biology》2015,83(2):300-311
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